plate reader
enspire multimode plate reader – a flexible and upgradeable multimode plate reader which includes the absorbance mode as standard. enspire alpha only plate reader - an affordable single mode reader for easy conversion from elisa to alphalisa ® and hts alphascreen ®
the envision 2105 xcite multimode plate reader – a single - detector system ideal for the most challenging research and assay development applications. the envision xcite multimode plate reader is upgradeable to the envision hts multimode plate reader .
a straight forward easy to use micro - plate reader serving us since for years .
thermo scientific™ remel elx800 calibration plate is designed for use with the elx800 microplate reader .
imark™ microplate absorbance reader the imark microplate absorbance reader offers fast reading speeds, extensive onboard software capabilities, a preprogrammed validation protocol (for the checkmark reader performance check plate) .
for use with cytation3 cell imaging multi - mode reader and synergy h1 hybrid microplate reader .
a straight forward easy to use micro - plate reader serving us since for years. read more
enspire plate reader is available outside the eu. please contact your local representative for further details .
astronaut kate rubins inserting a microplate into nanoracks plate reader - 2. image courtesy of nanoracks .
fluorescence plate reader > biophysical resource | w. m. keck foundation | yale school of medicine
the envision 2105 hts multimode plate reader - a super - fast, dual - detector reader designed specifically to support demanding high - throughput discovery applications .
checkmark™ reader performance verification kit the checkmark reader performance verification kit consists of an absorbance calibration plate and checkmark software for bio - rad absorbance microplate readers .
the envision multilabel plate reader - a super - fast, dual - detector reader designed specifically to support demanding high - throughput discovery applications. envision multilabel plate reader provides the highest speed, ultra high - thr... read more ...
upgradeable absorbance plate reader delivering sensitive results for elisas and low volume nucleic acid or protein quantification assays .
the aim of this experiment was to measure the amount of protein extracted using the bradford protein assay. the spectramax2 plate reader was used to detect the absorbance. this plate reader was... read review
tecan genios fl fluorescence microplate reader w warranty this is a good working tecan genios filter based fluorescence microplate reader. the genios fl reader offers fluorescence top meas ...
plate reader with dedicated optics designed to deliver exceptional results for glow, flash and dual - color luminescence applications .
molecular devices new spectratest fl1 fluorescence validation plate spectratest fl1 fluorescence validation plate a comprehensive tool for verifying ...
perkinelmer offers a variety of plate seals. topseal plate seals are applied to the top surface of the plate, and are generally used to prevent evaporation or contamination during assay incubation steps and / or plate reading measurements. note that topseal plate seal has spectral properties that may interfere with an assay measurement; you may need to remove the seal prior to reading your plate. backseal plate seals are applied to the bottom of the plate. backseal plate seals can be used to change a clear - bottom plate into a white - or black - bottom plate in order to reduce cross - talk during top - reading measurements .
backseal plate seal can be used to convert a clear - bottom plate into an opaque (solid - colored) plate for top reads. backseal plate seal is offered (catalog number 6005199 for white, catalog number 6005189 for black). the color of the backseal plate seal should match the color of the sides of the plate wells .
nasa image: iss048e044263 - the front panel of nanoracks plate reader - 2 after installation on the international space station .
tecan infinite f200 reader fluorescence reader this is a nice used good working tecan infinite f200 fluorescence filter based microplate reader. unit has been tested and it is in excellent ...
backseal plate seal can be used to convert a clear - bottom plate into an opaque (solid - colored) plate for top reads. backseal plate seal is offered (catalog number 6005199 for white, catalog number 6005189 for black). the color of the backseal plate seals should match the color of the sides of the plate well walls .
learn how molecular devices’ versamax microplate reader provides an affordable, tunable filter - based microplate reader for monochromator - based systems .
synergy & trade; ht multi - mode microplate reader warranty: 120 days model: siafr instrument / servicetype: plate reader for absorbance, fluorescence, luminescence instrument description ...
nanoracks plate reader - 1 was delivered to the international space station (iss) on htv3 and returned on spacex - 5. nanoracks plate reader - 2 was delivered via spacex - 9 and is currently available for use on the iss .
backseal plate seal can be used to convert a clear - bottom plate into an opaque (solid - colored) plate for top reads. backseal plate seal is offered in both white (catalog number 6005199 for white, catalog number 6005189 for black). the color of the backseal plate seal should match the color of the sides of the plate wells .
the enspire alpha plate reader provides proven alpha technology to your lab at an affordable price and from the alpha detection leader .
upgradeable luminescence plate reader with dedicated optics designed to deliver exceptional results for glow, flash and dual - color luminescence applications .
nasa image: iss034e005601 - the front panel of nanoracks plate reader - 1 prior to installation on the international space station .
the instrument is in pinn hall room 1074 and connected to the same computer used for the molecular devices fluorescent plate reader .
plate chameleon v microplate reader is an ideal tool for researchers who need a microplate reader to give them reliable, authenticated results when and where they need them. plate chameleon represents a new concept in multilabel detectio... read more ...
the fast, multichannel absorbance plate reader – with tunable wavelength and temperature control options – that can be tailor - made for your purposes .
nanoracks plate reader - 1 was previously on iss from september 2011 to january 2015; up on htv3 and returned on spx - 5 .
the advantage of using the fluorescent plate reader for fret measurements is that it easily and quickly delivers results and therefore is very useful for high troughput. furthermore, a plate reader is typically available in most standard biochemical laboratories and will therefore not require investment in new expensive hardware .
upgradeable absorbance plate reader delivering sensitive results for elisas and low volume nucleic acid or protein quantification assays. automated pathlength correction and low volume nanoquant plate deliver high precision results every day, independent from sample volumes .
envision® xcite multilabel plate reader is the single detection version of the envision reader designed for labs seeking ultimate sensitivity, the confidence of perkinelmer’s applications expertise and a broad portfolio of reagents with... read more ...
the ensight ™ multimode plate reader combines labeled & image - based cytometry technologies in a single benchtop platform. imaging & online data analysis is fast: a 384 - well plate can be imaged in less than 5 mins. the system’s modular design, combined with workflow - based kaleido ™ data acquisition & analysis software, gives a truly versatile plate reader that gets users productive quickly .
the most sensitive microplate reader for high - throughput screening read more ...
nanoracks plate reader is a modified spectramax® m5e multi - mode microplate reader. the spectramax® m5e multi - mode microplate reader is the standard for uv / visible multi - mode reader absorbance, providing ultrafast, full spectral range detection for 6 -, 12 -, 24 -, 48 -, 96 -, 384 - well microplates while utilizing the softmax pro 6 data acquisition and analysis software package. researchers can also use a cuvetteplate micro plate adapter for cuvette samples. nanoracks plate reader possesses five modes, uv - visible absorbance, fluorescence intensity, time - resolved fluorescence, and fluorescence polarization providing the benefit of multiple detection modes in one platform. the nanoracks plate reader has an added thermal regulation capability making long - term incubation of samples possible, such as measuring microbial growth or monitoring gene expression .
infinite® f50 innovatively designed, next generation, 8 - channel absorbance plate readers .
plate is washed three times with buffer (for example, 1x pbs) .
the two standard curves gave similar ec50 and hillslope values. the signal measured using a white plate was approximately 10 - fold greater than that from the black plate, and the s / b ratio was also better using a white plate .
spectramax m2 microplate reader notes: excellent physical and working condition. ready absorbance and fluorescence recent abs and fl calibration reports from a spectratest plate. i ...
the envision 2105 multimode plate reader provides exceptional speed, ultra - high throughput, and maximum sensitivity across all detection technologies. tried and tested, the envision microplate reader gives you robust performance and reliable data for high throughput screening, time after time .
there are now six application - tailored configurations for the acclaimed and proven infinite 200 pro plate reader, including new options such as automated pathlength correction. this allows you to choose the perfect reader for your specific needs and budget with a fast delivery time .
we list all our equipment in the parts category! abi perseptive biosystems, cytofluor multiwell plate reader 04741 discription of listing applied biosystems (abi) perseptive biosystems ...
we list all our equipment in the parts category! abi perseptive biosystems, cytofluor multiwell plate reader 03438 discription of listing applied biosystems (abi) perseptive biosystems ...
the infinite 200 pro is an easy - to - use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications .
dual - mode plate reader with filter - based optics for absorbance and fluorescence (top and bottom reading) applications. your cost - effective tool for highest demands in sensitivity .
the infinite 200 pro plate reader family is supplied with the easy - to - use i - control software interface, allowing you quickly define a workflow for each of your applications .
cytation™ 1 cell imaging multi - mode reader cytation™ 1 cell imaging multi - mode reader eliminates the complexities of multi - mode detection without compromising performance. it can be config ...
some plate readers handle label - free cell - based assays, such as perkinelmer’s enspire® multimode reader, which in addition to absorbance, luminescence, fluorescence and alphalisa also supports corning’s epic® technology. (in contrast, perkinelmer’s faster, top - of - the - line envision multilabel plate reader can read 12 modalities, though not epic, according to its documentation. )
you must remove topseal™ - a or any other plate seal prior to reading the assay .
perkinelmer’s portfolio of microplate readers are all equipped with multiple detection modes to meet the diverse assay requirements of today’s laboratories. no matter which one of our plate readers you choose, you can expect excellent performance and reliability. the variety of models and accessories, such as plate stackers, means there’s a perkinelmer plate reader to match both your throughput needs and your budget .
perkin elmer envision 2104 plate reader can be configured according to your needs. our systems are certified by our pe trained service engineer. units come with computer loaded with softwar ...
make the most of your high - quality data generated using the enspire multimode plate reader by harnessing the advanced data visualization and analysis capabilities of tibco spotfire ®. our spotfire ® analyst for multimode detection (mmd) package is tailored to the analysis of plate reader data and enables you to visualize, correlate, compare and analyze your data and share freely with your colleagues .
q. do you have any suggested plate coating protocols that i can use to bind my antibody / sample to the plate? a. high - bind plates can be coated using any standard plate - coating method. plates can be coated passively using the basic outline below :
dual - mode plate reader with monochromator - based optics for absorbance and sensitive fluorescence (top and bottom reading) applications. your adjustable tool, even for low concentration nucleic acid and protein quantification .
priced $ 10, 995. 00 biotek synergy 4 fluorescence absorbance microplate reader internal part number 13279 predecessor to: n / a condition: this biotek synergy 4 microplate reader is in good ...
eppendorf assay / reader microplates elevate the sensitivity of your assays with the eppendorf assay / reader plates to a higher level! the plates offer an excellent signal & ndash; to & nda ...
for convenience and optimal data quality, the nanoquant plate is the only low volume plate on the market that is 100% calibration free. this saves time and gives you consistent and reliable performance .
fully loaded multimode plate reader with monochromator - based optics, offering free wavelength choice and scanning capabilities. your ready - to - go workhorse for all standard absorbance -, fluorescence - and luminescence assays .
the biophysical resource at keck facility offers the fluorescence plate reader as an
open access
instrument that can be used by trained users. to schedule a training session please contact ewa folta - stogniew .
based on this customer experience and feedback, the infinite 200 pro microplate plate reader has been continuously engineered for excellence. this proven platform has now evolved into a budget - friendly reader family consisting of six application - focused configurations, delivering performance and value to every lab, every day, for every assay .
we have an excellent condition year 2009 tecan f200 infinite multi - mode 96 well plate reader available for sale. it & # 39; s been annually maintained and operates per factory oem specifica ...
webinar: synergy neo - a new hts multi - mode microplate reader designed for todays smarter ...
state - of - the - art eight - channel absorbance reader for accurate, reproducible and fast results .
the most versatile, high - performance monochromator - based multi - mode microplate reader read more ...
a streamlined workflow for cell counting and phenotypic characterization is critical to many experiments. our spectramax® i3x microplate reader with…
delfia assays require a plate reader that is capable of trf (time - resolved fluorescence) detection. this is a time - resolved fluorescence intensity assay. we recommend using a perkinelmer victor™ x or envision® multilabel plate reader (view our instrument product pages). if you are using a plate reader manufactured by another company, you can use our europium standard solution (catalog number b119 - 100) and perform a serial dilution in delfia enhancement solution to determine the sensitivity of your instrument. we also offer samarium standard solution (catalog number b115 - 100) and terbium standard solution (catalog number c558 - 100) if you are interested in multiplexing .
the well - to - well crosstalk measured in the various plate formats is shown in the table below .
mithras² lb 943 is a high - end microplate multimode reader based on monochromator technology with excellent performance. renowned for its sensitivity and robustness especially in luminescence and bret measurements the reader supports al... read more ...
clear bottom plates can be converted to functionally opaque plates by application of a backseal™ adhesive bottom seal. backseal plate seals are available in either white or black (catalog number 6005199 for white, catalog number 6005189 for black). the color of the backseal plate seal should match the color of the sides of the plate wells .
throughput, flexibility, and cost are the top three considerations when choosing a plate reader. in pharmaceutical research, for example, throughput might be the top criterion. regarding flexibility, a single - mode instrument, such as an absorbance reader for elisas, may fit the bill (some can capture a full uv - vis spectrum from 220 to 1000 nm), but a multimode reader that offers additional capabilities such as fluorescence may offset the additional cost outlay. integrated software, type of plates accepted, and built - ins like incubators and plate shakers are additional considerations .
incubation temperature - regulated heating plates above and below the entire plate movement area provide uniform incubation up to 45°c .
the infinite 200 pro is an easy - to - use multimode plate reader family that offers affordable high performance detection solutions empowered by monochromator - or filter based technologies. the six new tailored configurations provide excellence in elisa assays, nucleic acid quantifications, reporter assay technologies, and drug discovery assays including htrf® and fluorescence polarization. this fully upgradeable reader family is designed to simplify your target applications, making it easier than ever to choose the perfect reader for your needs and budget .
flash type luminescent assays require the use of reagent injectors to provide reagents that initiate the reaction while the plate is in the reader. use of these reagent delivery devices requires that the fluid path be maintained and free of contamination. reader models that do not have reagent injectors generally do not need very much maintenance beyond the guidelines outlined in the user’s manual .
this note describes the dual luciferase reporter assay performance using tecan’s infinite 200 pro multimode reader equipped with a two channel injector .
spectramax® absorbance microplate readers can run both cuvette - based and microplate reader applications on the same instrument. read one sample or up to 384 in a single plate read using any standard cuvette, or 96 - or 384 - well microplate .
this note describes the implementation of the infinite 200 pro multimode reader and the associated nanoquant plate for fluorescence - based dna quantification in small volume samples using pico green®, an ultra - sensitive fluorescent dye for the quantitation of double - stranded dna .
sometimes referred to as
solid phase assays
, coated - plate assays require the anchoring of one of the assay components (protein, antibody, sample, etc .) to the surface of the microplate. coated - plate assays use wash steps to separate bound (associating) and unbound (non - associating) reagents from the well of the plate .
as instrument requirements regularly change according to ongoing research needs, each of the six configurations can be upgraded to a full multimode monochromator - and filter - based reader. this ensures your infinite reader will deliver high quality results for every assay, every day .
fully loaded multimode plate reader with filter - based optics – offering highest sensitivity within the infinite 200 pro family, combined with the broadest range of read modes. your ideal system for low to medium throughput drug discovery, including htrf® and fluorescence polarization .
molecular devices spectramax m5 plate reader with pc and software includes 30 day money back guarantee! ! general specifications dimensions (in .) 8. 6 h x 22. 8 w x 15. 3 d dimensions (cm) 22 ...
it should be noted that the use of cells expressing sortilin and p75 ntr fused to cerulean and venus results in a pool of intracellular located proteins, which are most likely not partaking in the interaction with prongf. as the intracellular located proteins add to the fluorescent signal detected by the plate reader, the effect of prongf could therefore be expected to be underestimated. it is therefore important to test the plate reader result on a confocal microscope where the intracellular contribution to the fluorescent signal subsequently can be subtracted. however, by using cells stably co - transfected with sortilin - cerulean and p75 ntr - venus, the very same cell line can be used for several different fret - based techniques based on a plate reader, microscope, and flow cytometer .
don' t seal the plate with tape after the addition of the enhancement solution. the adhesive might quench the signal .
check that the wells are dry after washing. if not, invert the plate and tap it firmly against absorbent paper .
learn how the spectramax 190 microplate reader, a high - performance uv - vis spectrophotometer can be used for various life science applications .
bmg labtech’s nephelostar plus is truly in a class by itself. it employs a laser to detect forward scattering of light to insoluble particles in a sample. this process, laser based nephelometry, is not unique but incorporating this technology into a plate reader is !
our alpha and lance ® reagents are developed and optimized on the envision plate reader so you can be confident of accurate results. plus, we can cite over 15, 000 published papers that attest to its real - world performance across a broad range of applications .
the following plate readers can be configurated to perform luminescence measurements. choose between pherastar fsx - the high - throughput screening reader for fast read times, clariostar with lvf monochromators™ for highest flexibility and omega series - our upgradable microplate readers for luminescence or further dectection modes .
this technical note describes optimized test set - ups, instrument settings and the statistical equations that were used to determine the maximum instrument sensitivity of the infinite f200 pro filter - based microplate reader and the infinite m200 pro quad4 monochromators - based microplate reader for flash and glow luminescence .
learn more about the spectramax plus 384 microplate reader and how it can be used to test a breadth of applications with full spectral range .
listing #: tecaninfinitem1000platereader - 4443 product description: the tecan infinite m1000 pro multilabel microplate reader is versatile, modular, monochromato ...
listing #: moleculardevicesspectramaxm5 - 4812 product description: the molecular devices spectramax m5e multimode microplate reader offers you accuracy of a dedicat ...
the patented nanoquant plate was the first tool specifically developed for absorbance - based, small volume nucleic acid analyses in a multimode reader. it is useful for a broad range of applications, such as dna or rna quantification, nucleic acid purity assessment and labeling efficiency determination .
nanoracks plate reader is a laboratory instrument designed to detect biological, chemical or physical events of samples in microtiter plates. microplate readers are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations .
microplates for standard, in vitro fluorescent assays that do not require anchoring of cells or other reagents to the surface of the plate .
the following plate readers can be configured to perform fluorescence measurements. choose between pherastar fsx - the high - throughput screening reader for fast read times, clariostar with lvf monochromators™ for highest flexibility and omega series - our upgradable microplate readers for fluorescence or any number of dectection modes .
multimode microplate readers tend to be more expensive than single - mode readers—not surprising, given their more complex optics and detectors. biotek ’s simplest elisa reader, for instance, costs about $ 5, 000, says xavier amouretti, product marketing manager at biotek instruments, a company that sells a range of microplate reader systems; the company’s least - expensive multimode reader, the synergy™ ht, costs about $ 20, 000 .
the reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. the spectral resolution ensures accuracy of dna absorbance measurements .
discover how the spectramax® 340pc384 reader can be used in various absorbance - based applications by being able to accurately measure absorbance in the visible range .
synergy™ htx multi - mode microplate reader is a compact, affordable system. synergy™ htx multi - mode microplate reader is a compact, affordable system for 6 - to 384 - well microplates and take3 micro - volume plates, automating absorbance, f... read more ...
enspire ® multimode plate reader is designed to meet the demands of multi - user research environments, providing high performance technology and flexibility for a range of applications without compromising sensitivity. combine this with our expertise and portfolio of reagents, perkinelmer offers an unrivaled solution to your application needs .
bmg labtech launched the xenon flash lamp - enabled clariostar in mid - april. like the pherastar fs, clariostar is a multimode plate reader capable of performing absorbance, fluorescence intensity, fluorescence polarization, fret, time - resolved fluorescence, luminescence, bret and optionally alphascreen and alphalisa .
plate background luminescence: the white plates commonly used for luminescence experiments will often fluoresce. these plates absorb energy from the ambient light in the room and then emit the energy as light during the luminescence measurement. this phenomenon can be a problem if the plates have been exposed to bright light for a particularly long period of time. store unused plates in a dark environment and, when pipetting samples into the plate, do so in a reduced light in order to minimize plate autofluorescence. one can also “dark adapt” the plate by incubating it in the dark for approximately 10 minutes. the easiest way to accomplish this is to maintain it in the reader’s reading chamber prior to the initiation of reading .
in addition to the specific cell line being used in the assay, the assay protocol itself is important in deciding the type of plate to use. for example, assays using adherent cells may include culture medium changes or wash steps in the protocol. in such cases it may be advisable to use a coated plate for the assay in order to prevent the cells from becoming detached from the plate during the assay .
q. what kind of plate seal can i use for my plates? a. topseal - a adhesive plate seal (catalog number 6050185) can be used to prevent evaporation during incubation steps. the plastic of the topseal - a adhesive seal has some spectral properties that may interfere with assays performed at certain excitation or emission wavelengths. it may be necessary to remove the seal from the plate prior to reading .
the spectramax® l microplate reader measures flash and glow luminescence assays in both 96 - and 384 - well plates. it is upgradeable to include a barcode reader, programmable injectors, and dual - channel configurations for a 2 - fold increase for medium throughput. the high sensitivity, low - noise photomultiplier tubes (pmts), and variable - volume injector configuration make this reader ideal for fast kinetic assays requiring continuous monitoring during injection and measurement .
figure 3: top view of a 384 - well white proxiplate microplate (left) and view from underneath the same plate (right). the bottom of the wells is pushed towards the top surface of the plate to help increase signal while allowing use of low assay volumes .
biotek flx800 flx800tb fluorescence luminescence microplate reader unit powers on and works, used preiously at a local university. this item was removed fr ...
learn more about the spectramax l microplate reader and its sensitive, reliable optics and injectors, and how it can be utilized to improve your research .
bmg labtech’s pherastar fs multimode plate reader has “simultaneous dual - emission detection, ” or sde, capability. according to carl peters, an applications scientist at bmg labtech, that means the instrument can read two signals simultaneously, whereas the company’s newer, non - sde - enabled clariostar cannot .
still other instruments enable plate - based imaging assays. biotek’s cytation™3, for instance, is a multimode plate reader that also functions as a high - speed automated digital fluorescence microscope with brightfield capability. traditional microplate readers perform mostly biochemical assays—measuring how much light passes through or is emitted by a sample. that’s great for getting a sense of the population of cells or molecules in the well, but what about cell - to - cell variation ?
a trf filter fluorometer plate reader utilizes bandwidth filters in front of the fiber optic for excitation and in front of the detector for emission wavelength choice. the grating - type spectrofluorometers are generally less sensitive than filter fluorometers, and loss in sensitivity can cause issues in highly sensitive assays such as trf .
the bactiter - glo microbial cell viability assay was tested on the infinite f200 pro filter - based multimode reader, using the instrument’s highly sensitive luminescence module .
listing #: moleculardevicesspectramaxm2 - 4872 product description: the molecular devices spectramax m2 is a dual - monochromator, multi - detection microplate reader wi ...
perkinelmer offers custom microplate services, including bulk ordering, fast and flexible plate barcoding, biological plate coating (including poly - d - lysine, collagen, streptavidin coating, antibody coating, and other coatings on request), custom tissue culture - treatment, custom high protein binding treatment, custom sterilization of microplates, special packaging, and other microplate treatments. if you are interested in custom plate services, please contact our custom service team :
with the optional low volume nanoquant plate™ and cuvette port, this reader represents a perfect tool for dna / rna quantifications (a 260 / 280) and purity checks (a 260 / 230), allowing you to work with a variety of sample formats and volumes, from 2 µl to 1 ml .
the spectramax® abs microplate reader bridges the gap between the affordability of filter - based readers and the flexibility of monochromator - based systems. the spectramax® abs plus microplate…
the spectramax® abs microplate reader bridges the gap between the affordability of filter - based readers and the flexibility of monochromator - based systems. the spectramax® abs plus micro…
molecular devices gemini xs microplate spectrofluorometer this is a nice used molecular devices gemini xs microplate spectrofluorometer. unit is in overall excellent condition. reader power ...
luminescence detection can be performed on the pherastar ® fsx, clariostar ®, and the lumistar ®, fluostar ®, and polarstar ® omega plate readers. all of these luminescence plate readers are certified for dual - luciferase reporter assays (dlready ®) and utilize luminescence - optimized low - noise pmt (photomultiplier tube) detectors which provide a dynamic range of up to nine decades, depending on the reader. all readers can be equipped with high - precision reagent injectors .
whether or not it is necessary to use a sterile, tissue culture - treated plate depends on the length of time the cells are going to be in the assay plate. in some assays the cells are added to the microplate and the assay is completed within a few minutes to a few hours. in other cases cells are grown in plates at least overnight prior to performing the assay, or are treated with compounds for extended lengths of time. as a general recommendation, if the assay is going to be performed within a single working day a sterile, tissue culture - treated plate is not necessary. if the cells are going to be in the plate overnight or longer a sterile, tissue culture - treated plate should be used, and aseptic techniques should be followed .
flexible monochromator - based multi - mode microplate reader that can be turned into a high - performance hybrid system with the addition of a filter - based optical module .
bmg floustar galaxy fluorescence microplate reader 90 day warranty serial number: 403 - 0767 the galaxy model are fully automated microplate based readers which incorporates different mea ...
ultraviolet (uv) measurements in microplates became possible when molecular devices introduced the first uv - capable microplate reader. since then, microplate measurements of dna, rna, …
building on our family of benchtop multimode plate readers, the victor nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. the perfect microplate reader for everyday biochemical and cell - based assays, it measures at any wavelength, with top and bottom reading of all technologies. its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser environments. combine with our assays and reagents to get you started right away .
automation is a key feature in high - throughput screening. several bmg labtech microplate readers have been successfully integrated into various robotic systems. since all instruments have the same x - y dimensions and plate out / in position, once automation has been designed for one bmg labtech reader, the solution will work for all. all plate readers can be controlled through activex or dde interfaces. moreover, the ascii data output is the most versatile in the industry and works with most lims and automation platforms .
q. can i use a seal on my plates, or will that kill my cells? a. if cell viability is no longer an issue, topseal - a™ adhesive plate seal (catalog number 6050185) can be used to prevent evaporation during incubation steps. the plastic of the topseal - a adhesive seal has some spectral properties that may interfere with assays performed at certain excitation or emission wavelengths. it may be necessary to remove the seal from the plate prior to reading. if cell viability is an issue at the time a seal is needed, we recommend using sterile plate lids (if sterile practices need to be maintained) or breathable plate seal (if antibiotics / antifungals can be added to the culture media to prevent contamination). breathable plate seals are available from various suppliers, including nunc® and corning® .
plate is
blocked
overnight to cover the well surface area that remains (typically using bsa, sugars such as trehalose or casein, serum, etc. )
this note describes the use of the fluorescence microplate reader tecan infinite f200 pro or m200 pro, for detection of as lias 20 pg dsdna in a 200 μl assay volume .
this reader utilizes molecular devices' patented pathcheck® sensor to measure the depth (optical pathlength) of samples in a microplate. it also allows for the elimination of standard curves .
another consideration, says amouretti, is plate format. some instruments can handle only 96 - well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1, 536 - well plates. (that difference comes at a cost, amouretti explains, as higher plate densities require better optics and more sensitive detectors. )
get more from your microplate reader with magellan, the universal reader control and data analysis software from tecan. magellan supports the complete range of tecan microplate readers, enabling quick and easy measurement of samples for first - time and expert users alike. a versatile user interface and powerful data reduction capabilities enable the optimization of sophisticated experimental work, making routine tasks easy .
bmg labtech offers two forms of gas control on its microplate readers. the atmospheric control unit (acu) for the clariostar is a unique microprocessor - controlled unit that independently regulates both co 2 and o 2 inside the microplate reader. alternatively, the gas vent is a positive pressure gas purge through which the internal atmosphere of the microplate reader can be replaced .
q: can i stop at some point in my immunoassay? a: we strongly recommend not incubating enhancement solution overnight in the wells. delfia assays can be stopped after final washing (before addition of enhancement solution) and your plate can be stored for 1 day, 1 week, 1 year, 10 years, etc. when protected from dust by putting on a plate lid. if enhancement solution has already been added and the plate can' t be measured: 1) protect plate from dust (lid on but no tape when enhancement solution is in the wells); 2) allow solution to completely evaporate; 3) add enhancement solution when ready, to regain signal .
the raw fret signal obtained from the plate reader is contaminated by overlap of the donor emission spectrum with that of the acceptor (donor spectral bleed through (dsbt) ) and by direct excitation of the acceptor by the donor excitation light (acceptor spectral bleed trough (asbt) ). this contamination must be quantified and subtracted from the raw fret signal .
this technical note describes the successful implementation of the transcreener fluorescence intensity assay on tecan’s quad - 4 monochromator - based infinite m200 pro and tecan’s filter - based infinite f200 pro multimode reader .
the reader delivers high performance with round - bottom, flat - bottom or half - area well plates. dual wavelength readings report the actual absorbance at each wavelength making data analysis convenient .
users of molecular devices' spectramax® i3 multi - mode reader can even add an optional minimax™ imaging cytometer module, which enables such applications as cell counting, viability, and marker expression .
fluodia t70 is a filter - based high temperature fluorescence microplate reader. it is an excellent platereader for people who want to measure fluorescence intensity. as an outstanding fluorescence instrumentation company that has been in the market for over twenty years, pti has received frequent inquiries from our customers to develop a fluorescence microplate reader for them for over 15 years. at the beginning, we tried different approaches in order to provide our customers with a fluorescence microplate reader that would be an add - on unit to our existing systems and would surpass those microplate readers in the market in terms of its fluorescence measurement capability. after numerous attempts, pti finally came to the conclusion that it is technically impossible to develop a microplate reader as an add - on unit that can meet our company' s high standards for fluorescence measurements. so pti decided to join forces with otsuka electronics co. , ltd, a japanese scientific instrumentation company who had made numerous plate readers for various companies such as pharmacia on an oem basis. the combination of pti' s expertise in fluorescence measurements and otsuka' s expertise in microplate reader instrumentation brings to the market an outstanding new product: the fluodia t70 high temperature microplate reader under the trade name of fluodia t70 with both pti' s trademark and otsuka' s trademark (photal) .
eppendorf assay plates are optimized for measuring absorbance assays in the visible and uv range, fluorescence and chemiluminescence assays. solid plates are suitable for top - reading plate r ...
bmg fluostar galaxy multi - functional microplate reader condition: excellent price: $ 11, 999 warranty: 90 days the fluostar galaxy models are fully automated microplate based multi - detect ...
significantly improve the quality of your results with enhanced sensitivity in top reading fluorescence intensity mode. automatic z - focus adjustment determines the focal point for the best signal - to - blank ratio in each microplate well, regardless of the plate type, well shape or sample volume. this unique feature makes it easy to set up your reader for optimum performance with varying assay parameters .
in addition to standard absorbance measurements, the nanoquant plate is compatible with top reading fluorescence measurements (eg. for picogreen® or ribogreen® assays), improving your dna / rna detection limits .
verify the accuracy of your absorbance measurements with the spectratest® abs1 absorbance validation plate, using nist - traceable standards. testing can be done in your own lab, on your own schedule .
the spectramax® plus can read as low as 2μl with the μmax low volume plate. the spectramax® also uses patented pathcheck® pathlength measurement technology: the only temperature independent pathlength correction for microplates .
the optical design of the reader mimics a dual - beam spectrophotometer. each well has its own sample beam and reference detector. the eight - channel system delivers both precision and speed across the microplate .
this uv - visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96 - well, and 384 - well microplates, with the only temperature - independent method for pathlength correction .
decreasing the distance between cerulean and venus increases fret in the plate reader assay. a. schematic representation of used standard constructs. b. fret efficiencies obtained using hek293 cells transiently transfected with ctv, c5v, or co - transfected with plasmids encoding cerulean or venus (c + v) (n = 3). all data are presented as the mean ± sd. aa, amino acids .
this application note describes the detection of s. aureus growth using tecan’s infinite 200 pro microplate reader to perform 600 nm absorbance measurements. the influence of assay parameter and instrument settings on data quality is discussed .
held, p. (2003) peptide and amino acid quantification using uv fluorescence in synergy ht multi - mode microplate reader, biotek instruments application note, urltoken peptides - amino - acids - fluorescence. html
microplate readers for fluorescence, absorbance, luminescence, trf, htrf®, fluorescence polarization, and bret. two models available: tristar² lb 942 multidetection microplate reader with tristar²... read more ...
q. what kind of lids can i use for my plates? a. clear, non - sterile lids that fit our optiplate and proxiplate microplates can be ordered separately (catalog number 6005617 for 96 - well plates, catalog number 6007617 for 384 - well or 1536 - well plates). lids should be removed prior to reading the plate to avoid damage to the plate reader. lids for 96 - well plates have condensation rings that align with the underlying wells. these lids will leave a small space between the lid and the well, which can lead to evaporation over longer periods of time .
the nanoquant plate can simultaneously measure up to 16 samples, with sample volumes of as little as 2 µl. quartz micro - spots are shaped to function as individual lenses, guaranteeing the best possible light transmission through each sample. closing the plate lid effectively creates 16 quartz micro - cuvettes with a fixed path length of 0. 5 mm – perfect for 230, 260 and 280 nm measurements .
we understand your science and applications. combine many years’ experience developing and supporting multimode plate readers and reagents with a global network of application scientists and engineers, and you can be confident of minimal downtime .
fret analysis was performed using the pfret algorithm [ 19 ], which can be applied to regions of interest with sizes defined by the user, in principle down to the single - pixel level. it should be noted however, that the results acquired from the plate reader is calculated as an average over a population of cells within a well while the results presented from the microscopy measurements represents averages over 5x5 pixel .
the reader’s filter slides incorporate an id chip that allows filter definitions to be saved directly on the slides. this makes filter slide handling very convenient, as the system will automatically recognize the filters installed on each slide .
plate type: the type of plate used does matter. opaque white plates have been shown to provide the best performance for luminescence assays, as they maximize the signal. other plates can be used, such as white sided with clear bottom if necessary. solid black plates will also work, but the signal will be significantly reduced. quality plates should not allow any light leakage through the walls of the microplate .
q. what kind of lid can i use for my plates? a. most culturplate, tc - treated viewplate, and proxiplate plus tc plates are packaged with lids on the plates. sterile lids can also be ordered separately (catalog number 6005619 for 96 - well plates, catalog number 6007619 for 384 - well and 1536 - well plates). lids should be removed prior to reading the plate to avoid damaging the plate reader. lids for 96 - well plates have condensation rings that align with the underlying wells. these lids will leave a small space between the lid and the well. this is necessary so cells can ‘breathe’ when growing .
biotek offers two microplate reader series capable of measuring fluorescence: the synergy ht and the flx800. depending on the model and type the end user will be able to perform various luminescent reactions. for example, only those readers configured with injectors have the capability to perform flash luminescence assays, while virtually all the luminescent capable readers can perform glow assays. listed below are several different parameters, which may or may not apply to any one specific reader .
- venus. cells were seeded into 96 - wells plates two days prior to the experiment. medium was then carefully exchanged with pbs and prongf added directly to wells and incubated for 30 min. measurements were done at 20°c using a victor3, 1420 multilabel counter fluorescent plate reader from perkin elmer. again, low amounts of prongf i. e. 0. 05, 0. 5, 5 nm increased the interaction between sortilin and p75
a method is described for the routine determination of the rate of colorimetric enzyme reactions using a 96 - well microtiter plate reader commonly used in immunoassay. this approach is illustrated by monitoring esterase activity using three common products: release of thiol, release of ethanol, and release of p - nitrophenylate ion. examples include monitorings of the rate of hydrolysis of acetylthiocholine iodide by eel acetylcholinesterase and the rate of hydrolysis of malathion and nonconventional esters such as o - methyl, o - ethyl, and o - isobutyl carbonates of p - nitrophenol by commercial porcine liver carboxylesterase. data obtained from the plate reader were compared to those obtained, under similar conditions, in a conventional spectrophotometer. absorbance measurements made in both machines on the same solution, as well as absorbance changes measured over time, were similar. the use of the 96 - well plate format tremendously increased the number of enzyme assays carried out per person and the interface with a personal computer allowed rapid manipulation of the absorbance values to calculate the desired rate data. this approach should be generally applicable to many routine colorimetric assays in the research laboratory .
the optimal microplate for a cell - based assay will be dependent on both the specific cell line being used in the assay and the assay protocol itself. questions that need to be answered in choosing the plate include :
microplate readers present a dizzying array of variables – light sources, detectors, modalities, plate format and more. but when making a purchase, there are three basic considerations users should bear in mind, says amouretti .
the ez read led, explains product marketing manager tristana von will, “provides a really high - quality light source without ever having to replace it, and it also uses less energy, so it’s a much greener option, as well. ” (tecan ’s infinite® f50 absorbance reader also uses leds; according to michael fejtl, market manager for detection at tecan austria, “the led allows 10 - times longer maintenance - free lifetime of the reader. ” )
the nanoquant plate from tecan allows researchers to measure the concentration and purity of nucleic acids of up to 16 samples simultaneously. additionally, the efficiency of the nucleic acid labeling with dyes can be easily determined with this tool .
in this paper, we describe the use of a standard fluorescent plate reader in a fluorescent fret - based assay using live cells expressing the fusion proteins sortilin - cerulean and p75 ntr - venus. we show how this system can be used to evaluate the effect of prongf on the interaction between sortilin and p75 ntr. the fret based assay may provide a new tool for screening potential compounds inhibiting prongf - induced apoptotic cross - linking of sortilin and p75 ntr .
microplate readers designed for fluorescent and multi - detection measurements assist life science and drug discovery researchers who require repeated absorbance measurements of tagged molecules. considerations for readers are range of absorbance, sample holding capabilities, speed, and instrument size. sample detection requires appropriate filters; a microplate reader should include the lab’s most often used absorbance spectrum. if the desired absorbance spectrum is largely variable, as with fluorescence and luminescence spectrums, a flexible multi - mode instrument with several filters or change - out options may be appropriate. consider average sample run for choice of 96 - or 384 - well format. if microplate well size is interchangeable, consider investing in a microplate reader that can accommodate a multitude of plate types such as a 6 - to 384 - well format. instruments have become more compact to accommodate limited bench space without compromising reader intricacy or measurement speed. researchers can compare all of these specifications from our extensive collection of diverse microplate readers .
in conclusion, this paper describes the establishment of a fret - based fluorescent plate reader assay using live cells to monitor sortilin - p75 ntr cross - linking and the influence of prongf on this interaction. the experimental set - up may provide a useful tool for identifying compounds interfering with cross - linking of sortilin - p75 ntr. using this set - up, we have now initiated work aimed at identifying compounds inhibiting sortilin - p75 ntr cross - linking induced by prongf.
plate readers , also known as microplate readers or microplate photometers , are instruments which are used to detect biological , chemical or physical events of samples in microtiter plates . they are widely used in research , drug discovery , bioassay validation , quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations . sample reactions can be assayed in 6-1536 well format microtiter plates . the most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well ( 8 by 12 matrix ) with a typical reaction volume between 100 and 200 µl per well . higher density microplates ( 384 - or 1536-well microplates ) are typically used for screening applications , when throughput ( number of samples per day processed ) and assay cost per sample become critical parameters , with a typical assay volume between 5 and 50 µl per well . common detection modes for microplate assays are absorbance , fluorescence intensity , luminescence , time-resolved fluorescence , and fluorescence polarization . 
plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. they are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. sample reactions can be assayed in 6-1536 well format microtiter plates. the most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µl per well. higher density microplates (384 - or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µl per well. common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization.
plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. they are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations. sample reactions can be assayed in 6-1536 well format microtiter plates. the most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µl per well. higher density microplates (384 - or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with a typical assay volume between 5 and 50 µl per well. common detection modes for microplate assays are absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization.