blood car
at just 75 minutes, even a car on blood still runs out of gas .
a schoolteacher struggles to fuel his latest invention: a car that runs on blood .
in the future gas prices are $ 30 a gallon and one man invents a car that runs on human blood .
the aptly - named blood car is a near - future tale about peak oil and bloodthirsty vegans. gasoline is …
' blood car' is essentially a one - joke scenario that never quite adds up to the sum of its mangled body parts .
we measured the number of car - bcma + cells in the blood of all patients with a qpcr assay that was able to specifically detect car - bcma + pbmcs (figure 4a). although car - bcma + cells could be detected in the blood of all patients, the patients on the lower 2 dose levels had low levels of blood car - bcma + cells, and the patients with the most impressive antimyeloma responses, patients 8, 10, and 11, had the highest peak car - bcma + cell levels. by 3 months after infusion, blood car - bcma + cell levels had decreased to 0. 1% of pbmcs or less in all patients .
blood car may have been collecting prizes and earning praise for its supposedly wickedly twisted satire but it still seems trashy, b - movie junk .
the award winning debut feature from director alex orr, the pitch - black comedy blood car mixes surrealist humour, sharp social commentary, kinky sex and buckets of blood. starring anna chlumsky (best known for her charming starring role in my girl two decades ago), mike brune and katie rowlett, blood car is an independent horror - comedy gem that fully deserves to be rewarded with the cult status. blood car (cert. 18) is released by left films and will open at selected cinemas around the uk from 24th february 2012 .
blood car director: alex orr genre: dark comedy dvd street date: march 3, 2015 dvd srp: $ 19. 95 dvd upc: 738329162429
autologous peripheral blood mononuclear cells (pbmcs) were cultured with an anti - cd3 monoclonal antibody to induce t - cell proliferation. the cells were transduced with the γ - retroviral vector that encoded that car, and 9 days after the initiation of cultures, car - bcma t cells were infused. details of the car t - cell production are in supplemental methods, available on the blood web site .
in the not so distant future, gas prices have skyrocketed to a crushing 30 bones a gallon. the prices forces one good - natured vegan to get creative and find alternate means of fuel but instead of building a car that runs on veggies, he accidentally invents a car that runs on blood. now that' s the kind of gory thinking they should have used in the death race remake: more blood, less common sense. click through for the full blood car poster and trailer .
the effectiveness of anti - cd19 car t cells against b - cell malignancies encouraged us to develop a car t - cell therapy for multiple myeloma .
publisher' s note: there is an inside blood commentary on this article in this issue .
message body (your name) thought you would be interested in this article in blood journal .
vegan schoolteacher archie is trying to invent an alternative engine that runs on wheatgrass, but when he accidentally cut himself he discovers that all his engine needs to work is blood… human blood. with his blood - fuelled car, archie soon draws the attention of sex - crazed hottie denise, who will do anything for a ride. but government agents are spying on archie, as he is forced into ever more shocking and extreme behaviour to keep his blood car on the road, in this award - winning critically - acclaimed black comedy .
we had a great turn out for our second to last set of feature films for the festival blood car and disarm. both jack hammond of disarm and alex orr of blood car where on hand for the screening and answered audience questions. both movies were very appreciated by the audience and we hope to see both directors again in future festivals .
horizon movies is pleased to announce the dvd release of the cult festival hit blood car, a black comedy — directed by alex orr and starring mike brune, katie rowlett and anna chlumsky — set in a not - too - distant future in which the exorbitant cost of fuel drives one enterprising man to create a car engine that can run on human blood. combining elements of social satire and horror, sight and sound called blood car “a mordant, raunchy and at times gory trip into the dark heart of the american dream” .
see more matte vinyl car wrap silk brown 50ft x 5ft com ...
with the headline: film; the brothers coen bow in with'' blood simple'' .
the vast majority of cases of kidney failure are caused by complications of diabetes or high blood pressure .
in the near future, gas prices are at an astronomical high. one man is determined to find an alternate fuel source. that alternate fuel source turns out to be blood... human blood .
matte vinyl car wrap silk brown 50ft x 5ft comes with ...
veering between wicked social satire and outbursts of hardcore gore, blood car lives up to its reputation as an “audacious, utterly bonkers and downright sick claret - drenched black comedy” (time out london) .
26. 2 marathon runner vinyl decal sticker car window wall bumper running man 13. 1
in the very near future, no - one can afford to drive any more. fuel prices are at an astronomical high and car parks have become graveyards for abandoned cars. vegan school teacher archie is trying to invent an alternative engine that runs on wheatgrass, but when he accidentally cuts himself he discovers that all his engine needs to work is blood... human blood. with his blood - fuelled car, archie soon draws the attention of sex - crazed hottie denise, who will do anything for a ride. but the authorities are already following archie, as he is forced into ever more shocking and extreme behaviour to keep his blood car on the road, in this award - winning critically - acclaimed black comedy .
novartis: car - t results in one month, or no charge. why one month ?
car - bcma t cells became more differentiated after infusion. for a - l, cells from patient 10 are shown. all plots are gated on live lymphocytes, and the numbers on the plots are the percentages of cells in the indicated regions of the plots. staining for car - bcma was performed with pe - bcma - fc. all postinfusion samples were pbmcs that were collected 16 days after car - bcma t - cell infusion. (a) cells from the time of infusion (infusion cells) were stained for cd3 and car - bcma. (b) this plot is gated on the cd3 + car + infusion cells. (c) postinfusion cells were stained for cd3 and car - bcma. (d) this plot is gated on postinfusion cd3 + car + cells. car t cells after infusion were predominantly cd8 +. (e) ccr7 and cd45ra expression of cd3 + cd4 + car + infusion cells is shown. (f) ccr7 and cd45ra expression of cd3 + cd8 + car + infusion cells is shown. (g) ccr7 and cd45ra expression of postinfusion blood cd3 + cd4 + car + t cells. (h) ccr7 and cd45ra expression of postinfusion blood cd3 + cd8 + car + t cells. (i) cd3 + cd8 + car + infusion cell pd - 1 and cd28 expression are shown. (j) pd - 1 and cd28 expression is shown on postinfusion blood cd3 + cd8 + car + t cells. (k) cd57 and cd28 expression on cd3 + cd8 + car + infusion cells is shown. (l) cd57 and cd28 expression of postinfusion blood cd3 + cd8 + car + t cells is shown. for m - p, results are shown for all 4 patients with sufficient blood car - bcma + t cells from 7 to 16 days after infusion for analysis. gating and analysis were performed as described above in a - l. the results are all for live cd3 + car + cells. the symbols shown in m correspond to the same patients for m - p. (m) the cd8 + to cd4 + ratio of car - bcma t cells increased for all 4 assessable patients between infusion and the time the postinfusion samples were collected. the percentage of cd8 + car - bcma t cells with (n) em or emra phenotypes, (o) pd - 1 expression, and (p) cd57 expression increased in all 4 assessable patients between infusion and the time a postinfusion sample was collected .
car - bcma t cells can eliminate mm cells even when high concentrations of soluble bcma are present in the blood of patients (figure 4d). in addition to being an important finding for car - bcma t - cell therapy, this finding is important because many other promising car target antigens are also present in soluble form in blood and at tumor sites. 45, 46 serum bcma served as a tumor marker because substantial decreases in serum bcma occurred in the 3 patients with the most impressive antimyeloma responses (figure 4d) .
the city alleged contributory negligence based largely on the result on a standard test for blood alcohol. using a blood sample drawn from krall less than an hour after the collision, the test revealed a blood alcohol level of 0. 159. the illinois legal limit is. 10, said defense counsel gregory e. rogus of chicago' s segal mccambridge singer & mahoney .
when archie and denise are in the car in the beginning of the movie, the car runs out of gas, from a few seconds earlier until that moment you can clearly see outside there is woods, but in the next shot denise get out of the car right in front of a house .
efficacy and toxicity management of 19 - 28z car t cell therapy in b cell acute lymphoblastic leukemia .
the year is the future. you have a car and run people over but it' s ok because they' re like zombies or something. evil zombies. evil zombies created in a government - run conspiracy. and only one person can stop them. that person is you... and your car. in the end there' s a plot twist like where you were the zombie all along and stuff but you don' t know that yet. oh yeah your car is called the blood car! 2000 !
these results demonstrate for the first time that car t - cells targeting an antigen other than cd19 can induce complete remissions of a hematologic malignancy. importantly, we have shown that car - bcma t cells have powerful activity against mm that was resistant to standard therapies. these results should encourage further efforts to enhance anti - bcma car t cell therapies. the striking activity of anti - bcma car t cells against mm indicates that car t cells targeting bcma have great potential to be an effective new treatment of mm. further development of anti - bcma car t - cell therapies is a very promising area of research .
blood car by alex orr could be termed the anti - grindhouse. it hit all its notes perfectly, nailing and sending up the exploitation genre, with a dose of environmental topicality. in the film' s wildly inventive plot, an everyman who is tired of rising gas prices and the obscene profits of the oil conglomerates, decides to take matters into his own hands... . by building a blood car, a car that runs on blood (clearly more available than oil). in this campy instant cult film, orr mixes buckets - of - blood horror with social commentary and even a little sex for good measure. when our hero falls for the girl of his dreams, he must make a critical decision about how much bloodletting he will do in order to keep his automobile in good running order .
patients with the most impressive antimyeloma responses had the highest levels of blood car - bcma + cells. (a) car - bcma + pbmcs were quantified in the blood of patients by qpcr. patients treated on the 2 lower dose levels had low levels of car - bcma + cells in their blood (left). patients treated on the higher 2 dose levels had higher levels of car - bcma + cells in their blood (right). patients 8, 10, and 11 had the best antimyeloma responses and the highest levels of blood car - bcma + cells. the results are presented as the absolute number of car + pbmc / μl of blood. pre stands for pretreatment. (b) il - 6 was measured in the serum of all patients. patients 10 and 11 (right) had the most severe cytokine - release syndrome, and these patients also had the highest serum il - 6 levels. serum il - 6 levels of the other patients are shown on the left. note the different y - axis scales on the left and right graphs. (c) five days after car - bcma t cell infusion, serum levels of 15 cytokines were measured in the blood of patient 10 and patient 11. results are expressed as fold increase over pretreatment levels. (d) serum soluble bcma of all patients was measured by elisa pretreatment and posttreatment. the posttreatment serum samples were obtained 22 to 29 days after car t - cell infusion for all patients except patient 12. the posttreatment sample of patient 12 was obtained 13 days after car t - cell infusion. all patients had detectable serum bcma. in the patients obtaining the most impressive antimyeloma responses, patients 8, 10, and 11, serum bcma decreased posttreatment (right); in contrast, serum bcma did not decrease substantially posttreatment in the other patients (left) .
steven grillo and friends at the
harry potter and the half - blood prince
movie premiere on long island, new york .
and now we have conducted the first - in - humans clinical trial of anti–bcma - car t cells .
lineage switch as a relapse mechanism of pre - b acute lymphoblastic leukemia following cd19 car [ abstract ] .
krall v. city of des plaines - left - turning driver involved in crash with des plaines patrol car .
“it’s the future – like, two weeks from now, ” deadpans a suited government agent direct to camera in blood car ’s opening scene, eating crisps like a cinemagoer as he introduces “a story about a man… about his invention… about society… about you. ”
in a dystopian alternate 1999, a former cop is forced to take part in a death race in which cars run on human blood as fuel .
paige mcatee at a grocery store at midnight to get
harry potter and the half - blood prince
book on long island, new york .
this rumor claims that a university student in india died from too much co2 in his blood after drinking eight bottles of coca - cola too quickly .
i could probably sum up my entire review of this game by simply referring to it as
like the classic kill car, but actually fun to play .
you basically drive around and run people down, but this game loses the crappy rpg elements of kill car in exchange for blood particle physics and blood decals on the car that make it look like you' ve just strapped nails to your tires and driven through a playground full of ketchup - filled balloons. you would think that' s all this game would need, but i wouldn' t be wording it this way if the rest of the review was all sunshine and butt kissing .
car - expressing t cells targeting the b - cell antigen cd19 can induce lasting complete remissions of b - cell malignancies .
alex orr' s dark comedy blood car stars mike brune as archie andrews, a mild - mannered elementary teacher attempting to beat the high cost of gasoline (forty bucks a gallon) by creating a car that runs on something other than traditional petrol. he makes large purchases of wheatgrass from a young store clerk (anna chlumsky) who has a crush on the teacher. however, when he discovers that his new invention runs best on human blood, archie suddenly becomes involved with a woman who does not share his or the store - clerks environmental beliefs. ~ perry seibert, rovi
you might think strange title? put it in the dvd player, whowwww your in for a treat. very funny titles ie watch this - - - t more of this s - - t, so you know your in for a good time. we have watch it 3 times friends have done the same. the film is about petrol been about $ 15 a gallon, so if you got a car you are quids in with any hot chicks out there. nobody has cars due to this reason. so in comes along a teacher who by mistake invents a fuel to run on blood. so any thing with blood in goes in the boot gets liquidises up and runs the car for a short time. he has a fixation with a girl who loves his car but not him. great one liners, lots blood. serious cult flim. watch twin town aswell .
a naked, blood - soaked man throws a corpse into a dimly lit basement. the cadaver lands against another body. (source: imdb) read more »
seattle children’s is now aiming for car - t therapy not for the most desperate cases, but to treat, even cure, kids earlier in the course of the disease. the decision has also left novartis, which is charging $ 475, 000 per treatment, with no competition for the time being. cripe, chief of the blood cancer and bone marrow transplant division of nationwide children’s hospital in columbus, oh, sees other programs eventually giving doctors more options, especially more cost - effective “off the shelf” car - t therapies that use donor blood cells instead of a patient’s own cells .
toxicities that are mainly caused by cytokines (cytokine - release syndrome [ crs ]) also occur after car t - cell infusions .
we chose to focus on improving anti - bcma car t - cell therapies by simultaneously pursuing several different projects. we believe that optimizing car design is currently the most important way to improve car t - cell therapies; therefore, we are pursuing different projects aimed at finding the optimal design for anti - bcma cars. we are collaborating on a clinical trial of a 4 - 1bb–containing anti - bcma car. for the trial using the cd28 - containing car that is the subject of this report, we decided to administer different doses based on disease burden. because of the toxicities experienced by patients 10 and 11, we will only administer 9 × 10 6 car - bcma t cells / kg to patients with < 50% bone marrow plasma cells; patients with > 50% bone marrow plasma cells will receive 3 × 10 6 car - bcma t cell / kg. we are also undertaking a preclinical project that will examine the hinge and transmembrane regions of anti - bcma cars, and we are attempting to develop a fully human anti - bcma car .
although the response rates for anti - bcma car t cells against mm reported here are not as high as previous reports of anti - cd19 car t cells against b - cell malignancies, 16, 17, 19, 25 the number of patients treated with anti - bcma cars is quite small. compared with the doses of anti - cd19 car t cells generally required for efficacy and toxicity in our experience (1 - 2 × 10 6 / kg), 19 higher doses of anti - bcma car t cells (3 - 9 × 10 6 car + t cells / kg) were required to elicit substantial antimalignancy responses and toxicity. this difference could be due to many factors including weaker bcma expression on malignant cells in comparison with cd19, differences in car design between anti - cd19 cars used previously and the anti - bcma car used in this work, and the presence of soluble bcma in serum and at tumor sites .
the anti - bcma car used in this work (car - bcma) incorporated the 11d - 5 - 3 anti - bcma single - chain variable fragment (scfv), a cd28 costimulatory domain, and the cd3−ζ t - cell activation domain (figure 1a). 12 the car sequence was expressed by a γ - retroviral vector backbone used in previous work. 11, 14 car - bcma was consistently expressed on the surface of transduced cd4 and cd8 t cells, and the transduced t cells proliferated extensively in culture (figure 1b - c; supplemental tables 1 and 2). a median of 67. 0% (range, 45. 3 - 90. 8 %) of infusion t cells expressed car - bcma. car - bcma t cells specifically recognized bcma in vitro (figure 1d) .
early - phase clinical trials have shown high rates of complete remission (cr) after cd19 car - t - cell therapy, even in patients who have failed multiple chemotherapy regimens and / or allogeneic hematopoietic stem cell transplantation (hct). durable remissions can be achieved when functional car - t cells persist sufficiently long to eradicate all malignant cells in the recipient; however, leukemic relapse may occur with premature loss of cd19 car - t cells or because of the emergence of leukemia that has lost cd19 expression despite persistence of functional cd19 car - t cells .
cd19 - targeted car - t - cell therapy of patients with mll - rearranged b - all effectively induced marrow remission of b - all .
upon its completion, blood car was given a quick and limited dvd release in the u. s. , even as it continued to win acclaim on the international festival circuit. largely overlooked in the states, it was embraced by uk critics as “a slacker take on roger corman movies like the little shop of horrors or death race 2000” (empire). now, seven years later, horizon movies and kino lorber re - introduce blood car to the american viewer in a special new edition, so that this loony yet scathing satire of a gas - guzzling nation on the verge of social collapse can finally gain the domestic notoriety it deserves .
anyone who is interested in detailing a car, cares about their car. who in their right mind would ever think of installing any material that is not built for automotive application! ?. there is a disconnect with info between what you can use and what you can' t .
two teddy - bears go hunting unicorns, their favourite prey. unicorns have tender flesh and delicious blueberry - flavoured blood which the bears need to stay cute - looking. read more »
we' ve shown you footage from blood car before and i' ve gotta say this looks like a great c - film. especially since it brings my girl' s anna chlumsky' s into horror. the flick has been popping up in film festivals across the us, but no wide release date has been announced yet .
both chemotherapy agents were administered daily on days −5, −4, and −3 before car - bcma t - cell infusion on day 0. a single dose of car - bcma t cells was administered to each patient. the dose escalation plan called for an initial dose of 0. 3 × 10
a schoolteacher struggles to fuel his latest invention: a car that runs on blood. after exorbitant gas prices force average americans to seek alternative fuel sources, archie overlooks his vegan beliefs and starts taking desperate measures to help power his new ride. will either of the two women in his life - butcher stand owner denise or veggie stand owner lorraine (anna chlumsky) - end up quenching the blood car' s thirst? alex orr' s clever horror comedy is part christine, part little shop of horrors. one thing' s certain: you' ll be glad you' re safe at home... contains strong language, sexual scenes, violence and gore .
loss of normal plasma cells after car - bcma t - cell infusions is an expected toxicity because normal plasma cells express bcma. 12 the low polyclonal immunoglobulin levels of patient 11 show that, at least in some cases, immunoglobulin replacement will be indicated in patients receiving anti - bcma car t cells .
a mosquito falls madly in love with a vampire. together they go looking for fresh blood in a baroque, bizarre and dangerous world... (source: lifff) read more »
appropriate target antigens for car t - cell therapies should be uniformly expressed on the malignancy to be treated and should not be expressed on normal essential cells .
we demonstrated that car - bcma t cells can eliminate large burdens of treatment - resistant mm. the prior treatment histories of patients 10 and 11 demonstrated the chemotherapy resistance of their mm. prior to enrollment on the car - bcma protocol, patient 10 only obtained a pr after 6 cycles of cybord therapy, and he had 90% bone marrow plasma cells 3 months after high - dose melphalan and asct. high - dose melphalan is a much more effective antimyeloma therapy than the low - dose cyclophosphamide administered immediately prior to car - bcma t - cell infusions. patient 11 had mm that was refractory to cybord therapy. fludarabine, which was administered prior to car - bcma t - cell infusions, has been shown to have essentially no activity against mm. 42 all in all, the antimyeloma responses after car - bcma infusion in patients 10 and 11 cannot be attributed to the chemotherapy administered prior to car - bcma t - cell infusions .
blood car is set to street on dvd march 3, with a srp of $ 19. 95. the disc comes packed with bonus features including two shorts from the fake wood wallpaper film collective, “the adventure” (2008, 21 min .) and “the $ 100 short short” (2006, 10 min .) as well as the theatrical trailer .
patients with mll - rearranged b - all who attain cr after cd19 car - t - cell therapy may be at risk for relapse with clonally related aml .
a bodybuilder, extraterrestrials, kidnapping, 40 gallons of blood—this is a beautiful, sometimes silly trip shot in spain in stunning black & white. (source: new orleans film society) read more »
for all 12 patients, the median ratio of cd8 + to cd4 + car + t cells in the infusion cells was 1. 1 (range, 0. 3 - 10. 9; supplemental table 4). sufficient car - bcma t cells were available for a phenotypic analysis of blood t cells from 4 patients after infusion (figure 5). among these 4 patients, the median ratio of cd8 + to cd4 + car + t cells in the infusion cells was 0. 9. in contrast, the persisting car + t cells in the blood of the same patients after infusion were mainly cd8 + t cells with a median cd8 + to cd4 + ratio of 28. 8 (range, 2. 5 - 60. 9; figure 5m). the expression of c - c chemokine receptor type 7 (ccr7) and cd45ra is used to divide t cells into 4 categories: naïve, central memory (cm), effector memory (em), and effector memory ra (emra). compared with naïve and cm t cells, em and emra t cells are more differentiated with less proliferative potential. 38 ccr7 and cd45ra expression data of the infusion car - bcma t cells of all patients are in supplemental tables 5 and 6. programmed cell death protein - 1 (pd - 1) is an inhibitory t - cell receptor important in t - cell exhaustion. 39 cd57 is a marker of highly differentiated t cells with reduced proliferative capacity. 40, 41 in the 4 patients with sufficient car + t cells for phenotypic analysis of blood t cells after infusion, car t cells acquired a more differentiated phenotype with an increase in em and emra t cells after infusion (figure 5n). increases in expression of pd - 1 and cd57 provided further evidence that car - bcma t cells acquired a highly differentiated phenotype after infusion (figure 5o - p) .
this is a decent comedy horror that has a unique premise, but could have been done better. the idea of the film is quite good, and the creative aspect is sure to delight horror fans looking for an amusing, mindless film to watch. however, i felt that blood car could have benefitted from a few rewrites as the concept is very good, but it does fall short of what the film could have been. the problem with the film is that it tries to rush things while trying to grab your attention. if this would have been a slightly longer feature, with a bit more depth to its sheer ridiculous plot, then blood car would really have been a great horror comedy. however it does fall short. i' m not saying it' s a bad film, but it' s a film that needed some improvements. despite its imperfections, this is somewhat enjoyable for what it tries to accomplish, and viewers looking for a fun, entertaining low budget b movie, will most likely enjoy this low budget affair. blood car could have been better, but for what it is, it' s a decent film worth seeing if you love ridiculous horror films. for the most part, blood car works, but there are better b movies out there. i really liked the concept; i simply wished that the filmmakers would have made the film a bit longer, in order for the story to have a nice flow. as it is, it' s a rushed film, one that doesn' t reach its full potential and it' s a shame because blood car could really have stood out. this is a decent film, but there is nothing remarkable about this b movie, but it' s worth checking out if you have nothing else better to watch .
a young girl wakes up in the trunk of a car. this is not how she imagined her birthday... (source: imdb) read more »
within 1 month you will receive a free car. a draw has just been concluded in hong kong last weekend (we contacted you via your email address) .
during the first week after car - bcma infusion, we noted a striking decrease in patient 10’s serum iga level (figure 2g). serum iga became undetectable and remained undetectable 14 weeks after car - bcma t - cell infusion. a dramatic eradication of mm from the bone marrow occurred. ihc for the plasma cell marker cd138 showed a complete absence of bone marrow plasma cells 4 and 8 weeks after car - bcma t - cell infusion (figure 2e - f). four weeks after car t - cell infusion, a population of t cells was detected in the bone marrow (figure 2h); 31% of bone marrow t cells expressed car - bcma by flow cytometry (figure 2i). the serum and urine immunofixation electrophoresis tests for monoclonal iga - κ became negative after treatment and remained negative 14 weeks after car - bcma t cell infusion. fourteen weeks after car - bcma t - cell infusion, multicolor flow cytometry did not detect mm cells in the bone marrow, which confirmed a stringent complete remission (cr). as expected, normal plasma cells were also absent from the bone marrow 14 weeks after car - bcma infusion. nineteen weeks after infusion, the serum immunofixation electrophoresis remained negative, but a monoclonal iga - κ protein was detected in the urine. this indicated a relapse of mm. subsequently, bcma + malignant plasma cells were detected in the bone marrow; car - bcma t cells were not detected in the bone marrow at the time of relapse .
after receiving cd19 - targeted car - t cell therapy, all patients with mll - b - all achieved cr in the marrow by flow cytometry; however, 2 of 7 patients relapsed, both with a myeloid phenotype leukemia approximately 1 month after car - t cell infusion. pathology findings are summarized in table 1 and figure 1 .
patient 10 experienced pancytopenia associated with a hypocellular bone marrow (figure 2e). he had an absolute neutrophil count (anc) of 370 immediately before the car - bcma t - cell infusion. the anc remained < 500 for 40 days after car - bcma t - cell infusion, and the patient had an episode of bacteremia associated with transient hypotension during this neutropenic period. the patient also experienced transfusion - dependent thrombocytopenia for 9 weeks after car - bcma infusion. fourteen weeks after car t - cell infusion, patient 10 had a normal anc, and he had not required a platelet transfusion in > 6 weeks, although his platelet count remained decreased at 43 000 / μl .
it' s inspiring to see films like this every once in a while - hyper low - budget triumphs of the indie spirit. occasionally hilarious and usually entertaining, except for a few kind of boring patches, blood car is an entertaining testament to the successes that independent film making can produce. it' s one of those movies that makes you want to go out and film your own .
car - bcma t cells specifically recognized bcma in vitro and exhibited antimyeloma activity in humans. (a) diagram of the msgv - 11d5 - 3 - cd828z γ - retroviral vector encoding the anti - bcma car (car - bcma) is shown. car - bcma contained the 11d5 - 3 anti - bcma single chain variable fragment (scfv), cd8α hinge and transmembrane regions, the cytoplasmic portion of the cd28 costimulatory moiety, and the cd3ζ t - cell activation domain. (b) car - bcma expression on the surface of the infusion t cells of patient 10 was detected by staining with a pe - bcma - fc protein reagent. the plot is gated on live cd3 + lymphocytes. (c) demonstrating specificity, the pe - bcma - fc reagent did not stain pbmcs that were not transduced with the car - bcma gene. (d) car - bcma t cells of patient 10 were cultured with target cells that either expressed bcma (bcma - k562 and rpmi8226) or did not express bcma (ngfr - k562 and ccrf - cem). the car - bcma t cells of patient 10 specifically released ifnγ in response to bcma - expressing target cells in vitro. (e) patient 8 who had mm that was progressing despite 8 prior lines of therapy obtained a very good partial remission (vgpr) after infusion of car - bcma t cells. positron emission tomography–computed tomography scans from before and after treatment show elimination of a large number of mm bone lesions. (f) patient 8’s free κ light chains level decreased after car t - cell infusion as the mm entered a vgpr and then increased with progression of the mm. the serum bcma protein level followed a pattern similar to that of the serum free κ light chains .
administration of lymphodepletion chemotherapy followed by cd19 - specific chimeric antigen receptor (car) –modified t cells is a remarkably effective approach to treating patients with relapsed and refractory cd19 + b - cell malignancies. we treated 7 patients with b - cell acute lymphoblastic leukemia (b - all) harboring rearrangement of the mixed lineage leukemia (mll) gene with cd19 car - t cells. all patients achieved complete remission (cr) in the bone marrow by flow cytometry after cd19 car - t - cell therapy; however, within 1 month of car - t - cell infusion, 2 of the patients developed acute myeloid leukemia (aml) that was clonally related to their b - all, a novel mechanism of cd19 - negative immune escape. these reports have implications for the management of patients with relapsed and refractory mll - b - all who receive cd19 car - t - cell therapy .
in a near future where gasoline costs more than $ 30 a gallon, schoolteacher archie andrews invents an engine that runs on human blood. needless to say, complications arise when his revolutionary creation requires fresh supplies of fuel .
patients received lymphodepletion chemotherapy, followed by infusion of autologous cd19 car - t cells, according to 2 clinical trial protocols (nct01865617, nct02028455). t cells were modified using a car construct incorporating a truncated human epidermal growth factor receptor as a marker of transgene expression, which enabled enumeration of car - t cells. 8 evaluation of leukemic blasts by flow cytometry, fluorescence in situ hybridization (fish), g - banded karyotyping, and chromosomal genomic array testing (cgat) was performed in clinical laboratory improvement amendments–certified laboratories, and deep sequencing of the igh gene was performed by adaptive biotechnologies (seattle, wa). quantitative polymerase chain reaction for vector sequence was used to enumerate integrated car transgene in car - t cells and myeloblasts. these studies were conducted with approval of the institutional review boards of fred hutchinson cancer research center and seattle children’s hospital and in accordance with the declaration of helsinki .
he sandal was reportedly found in 2012 at the site where konstantinos
dino
barkas was - operating a digger, while the car was - discovered last year at another spot .
so, with this flagrant breach of both chronological logic and the fourth wall, blood car immediately establishes its absurdist tone. alex orr’s feature debut was in fact completed in 2007, winning acclaim and awards at various festivals before going straight to dvd in the us – but in the half decade that it’s taken to get its theatrical release in the uk, the film has left its influence on quentin dupieux’s left - field ‘road movie’ rubber and dean francis’s more conventional highway horror road train (both 2010), the former similarly featuring a killer car part, the latter a carnivorous engine .
we detected soluble bcma in the serum of all 12 patients before treatment (figure 4d). serum bcma decreased after car - bcma t - cell infusion in the patients with the most impressive antimyeloma responses, patients 8, 10, and 11; in contrast, serum bcma did not decrease substantially after car - bcma t - cell infusion in the other patients .
six civilian witnesses testified that the overhead lights on officer neubauer' s car were not turned on, while three city police officers, including neubauer, testifies that the lights were operating .
all patients experienced cytopenias attributable to the conditioning chemotherapy that patients received on this protocol and the extensive prior therapy patients had received before protocol enrollment; patients 10 and 11 had cytopenias clearly more prolonged than expected from the chemotherapy that they received. toxicities are listed in table 2. toxicities attributable to car - bcma t cells were minimal in patients treated on the 0. 3 × 10 6 car + t cells / kg dose level. on the 1 × 10 6 car + t cells / kg dose level, patients 4 and 5 had signs and symptoms of crs including mild fever and tachycardia. patient 8 had significant fever, tachycardia, and hypotension among other toxicities, but the 3 other patients receiving 3 × 10 6 car + t cells / kg had mild toxicity. on the 9 × 10 6 car + t cells / kg dose level, patients 9 and 10 both had a variety of grade 3 and 4 toxicities .
when the transplant failed about three months later, “we thought it was a death sentence, ” says andy. but greta qualified for experimental car - t therapy, available only to patients with no other recourse. in august 2014 she received a dose of her own t cells, extracted from her blood, modified and expanded outside her body, then infused back into her bloodstream to zero in on the life - threatening cancer .
greta and charlotte were chattering in the back seat of the family car when i spoke to her parents recently. they were driving from their home in portland to a weekend vacation in canada .
to receive your free car all you need to do is send us your 1. full name 2. address / contact number 3. country of origin 4. occupation 5. email .
patient 11’s serum igg - λ m - protein started to decrease after car - bcma t - cell infusion and decreased to the point that it was not quantifiable at last follow - up 28 weeks after car t - cell infusion. the only evidence of mm at last follow - up was the serum igg - λ m - protein that was detectable only by immunofixation electrophoresis. the serum free λ light chains that were another marker of the patient’s mm became undetectable early after treatment (figure 3g). patient 11’s most recent formal myeloma response was vgpr. a bone marrow biopsy 4 weeks after the car t - cell infusion revealed a hypocellular bone marrow and complete elimination of bone marrow plasma cells (figure 3e). the patient was platelet transfusion dependent from day 2 until day 27 after car t - cell infusion. eight weeks after the car t - cell infusion, bone marrow cellularity was recovering, and plasma cells remained completely absent (figure 3f). absence of mm cells and normal plasma cells from the bone marrow was confirmed by multicolor flow cytometry 4, 8, and 25 weeks after car t - cell infusion. four weeks after car t - cell infusion, a large population of t cells was detected in the bone marrow (figure 3h); 19% of bone marrow t cells expressed car - bcma by flow cytometry (figure 3i). less than 8 weeks after the car t - cell infusion, all of patient 11’s toxicities except decreased lymphocyte count had improved to grade 1 or resolved, and he had returned to full - time employment. in accordance with continued absence of plasma cells in patient 11’s bone marrow, the serum immunoglobulin levels steadily decreased after car - bcma infusion. twenty - eight weeks after car t - cell infusion, the serum igg was 388 mg / dl (normal range, 700 - 1600 mg / dl), the serum iga was below detectable limits, and the serum igm was 15 mg / dl (normal range, 40 - 230 mg / dl). because of the low immunoglobulin levels, we initiated immunoglobulin replacement with intravenous igg .
'' blood simple'' contains much blood and gore, and the coens say they are surprised the critics haven' t taken them to task for the violence.'' but the violence is bound up in the story, rather than gratuitous,'' joel said.'' it' s very different from a horror movie where the characters are mechanically set up to be slaughtered. we have no slasher scenes. this is more of a story movie.''
making the most of his low budget and fully embracing a b - picture sensibility, orr loads his film with broad caricatures, low humour and hilariously surreal (and politically incorrect) digressions. but this is an exploitation movie about exploitation, and its disarming goofiness and transgressive vulgarity eventually give way to images of state execution and infanticide – all in the name of the american dream – that are shockingly serious, if still played for uncomfortable laughs. a speech of frankly insane justification delivered by another government agent (matt hutchinson) near the film’s end evokes dr. strangelove (1964) – and indeed blood car is similarly apocalyptic in its lampooning of post - millennial anxieties, with a gleefully cynical finale pointing to a dead - end future for all but a self - serving political elite. a couple more weeks down the road, blood car will be a cult classic .
this rumor has taken on many forms, claiming that coca - cola, due to its acidic nature, can be used to clean toilets and corrosion from car batteries, loosen a rusted bolt and remove rust spots from car bumpers, remove grease from clothing, clean road haze from windshields, clean highways after traffic accidents, cook a steak, dissolve teeth, and bake a moist ham .
but the novartis car - t wasn’t what cured greta. she was too young at the time to enter the experimental trial for that program. instead, her parents enrolled her in a study for a different car - t program, at seattle children’s hospital, which was accepting patients as young as a year old. greta (pictured above, with her sister charlotte, 6, behind her) first had to put on weight—lots of french fries and milkshakes, says her mother, maggie—then go through another round of chemo, but she managed to hang on for her car - t treatment .
progression of bcma - negative mm is a potential problem after car - bcma t - cell therapies. we assessed bone marrow mm cells for bcma expression after car - bcma t - cell infusion by ihc; the before and after treatment bcma expressions are summarized in supplemental table 4. a partial loss of bcma expression by malignant plasma cells was detected only in patient 7 (supplemental figure 1) .
the city of des plaines alleged that debra krall was under the influence when she made a left turn into the path of a police car driven by officer lori neubauer on sept. 23, 1995 .
patient 11 received 5 prior lines of therapy before enrolling on the car - bcma protocol. his most recent therapy prior to enrollment was cybord; during his only cycle of this treatment, patient 11’s mm progressed. before car - bcma infusion, 80% of the cells in the patient’s hypercellular bone marrow were bcma + plasma cells (figure 3a, b, d). four hours after infusion of 9 × 10 6 car - bcma t cells / kg, he developed a sustained fever that was associated with an elevated crp (figure 3c). worsening tachycardia, hypoxemia, and dyspnea developed over the next day, so 25 hours after the car t - cell infusion, tocilizumab was administered. this patient also had grade 3 delirium without other neurologic toxicities. four hours after the tocilizumab was administered, the dyspnea improved, and the temperature and heart rate decreased temporarily. a second dose of tocilizumab was administered 5 days after the car t - cell infusion. patient 11 had adrenal insufficiency at the time of protocol enrollment, and he received stress - dose intravenous hydrocortisone at doses varying from 25 to 75 mg every 8 to 12 hours between day 0 and day 13 after car t - cell infusion .
car - bcma t cells were detected by flow cytometry after staining with a phycoerythrin - labeled bcma constant - fragment reagent (pe - bcma - fc) (figure 1b - c). car - bcma t cells were also detected by performing quantitative pcr (qpcr). enzyme - linked immunosorbent assays (elisa) for interferon γ (ifnγ) were performed on supernatants from cultures of car - bcma t - cell samples plus target cells. interleukin - 6 (il - 6) elisas, soluble bcma elisas, and multicytokine assays were performed on patient serum. methods for all assays are in supplemental methods .
garry shandling died from a blood clot, tmz reported on tuesday after it obtained a copy of the comedian’s autopsy report. the outlet said that shandling also had xanax, hydrocodone and oxycodone in his system at the time, attributed to a recent dental surgery .
when i say
two of three bonus levels i saw ,
that' s because i couldn' t get very far in the game. i couldn' t find a certain npc on the second level which spans the entire city and i ultimately decided i wasn' t willing to put up with the hide - and - seek games, indestructible obstacles, and crappy car physics to find how many more inventive parallels the developers decided to draw between blood and feces .
immunotherapy with t cells that are genetically modified to express a cd19 - specific chimeric antigen receptor (car) represents a promising approach for patients with refractory b - cell acute lymphoblastic leukemia (b - all) .
we have previously shown that anti - cd19 car t cells administered without chemotherapy can cause cytopenias in patients with high disease - burden all. 23 of the patients receiving car - bcma t cells, patient 10 experienced prolonged pancytopenia and patient 11 experienced prolonged thrombocytopenia. we hypothesize that the cytopenias experienced by these 2 patients were a cytokine - mediated phenomenon caused by high levels of cytokines produced when car - bcma t cells contacted high burdens of bone marrow mm; however, bystander perforin / granzyme - mediated killing of hematopoietic cells could also be speculated. both of these patients were cytopenic prior to car - bcma t - cell infusion, which probably contributed to the severity of the cytopenias after car - bcma t - cell infusion (supplemental table 7). the chemotherapy that patients received as part of this protocol is expected to cause cytopenias, and the extensive therapy that the patients had received prior to protocol enrollment probably contributed to chronic bone marrow impairment in some patients. we never observed bcma expression on any bone marrow cells except plasma cells after performing ihc staining on bone marrows from 85 patients.
blood car is a 2007 black comedy film set in the not-too-distant future where gasoline prices have become exorbitant ( $ 30 + us/gallon ) . things take a turn for the worse when a kindergarten teacher ( mike brune ) invents a car that runs on a different fuel source : blood . 
blood car is a 2007 black comedy film set in the not-too-distant future where gasoline prices have become exorbitant ($ 30 + us/gallon). things take a turn for the worse when a kindergarten teacher (mike brune) invents a car that runs on a different fuel source: blood.
blood car is a 2007 black comedy film set in the not-too-distant future where gasoline prices have become exorbitant ($ 30 + us/gallon). things take a turn for the worse when a kindergarten teacher (mike brune) invents a car that runs on a different fuel source: blood.