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  <div style="text-align: center; color: #000000; font-weight: bold;">As filed with the Securities and Exchange Commission on April 1, 2025</div>
  <div style="text-align: right; color: #000000; font-weight: bold;">Registration No. 333-</div>
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    <div style="text-align: center; color: rgb(0, 0, 0); font-size: 14pt; font-weight: bold;">UNITED STATES</div>
    <div style="text-align: center; color: rgb(0, 0, 0); font-size: 14pt; font-weight: bold;">SECURITIES AND EXCHANGE COMMISSION</div>
    <div style="text-align: center; color: rgb(0, 0, 0); font-size: 12pt; font-weight: bold;">Washington, D.C. 20549</div>
    <div style="text-align: center; color: rgb(0, 0, 0); font-size: 18pt; font-weight: bold;">FORM S-8</div>
    <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">REGISTRATION STATEMENT</div>
    <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">UNDER</div>
    <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">THE SECURITIES ACT OF 1933</div>
    <div style="text-align: center; color: rgb(0, 0, 0); font-size: 24pt; font-weight: bold;">Bionano Genomics, Inc.</div>
  </div>
  <div style="text-align: center; color: #000000; font-weight: bold;">(Exact name of registrant as specified in its charter)</div>
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          <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">Delaware</div>
        </td>
        <td style="width: 2%; vertical-align: top;">&#160;</td>
        <td style="width: 49%; vertical-align: top;">
          <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">26-1756290</div>
        </td>
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        <td style="width: 49%; vertical-align: top;">
          <div style="text-align: center; color: #000000; font-weight: bold;">(State or other jurisdiction of</div>
          <div style="text-align: center; color: #000000; font-weight: bold;">incorporation or organization)</div>
        </td>
        <td style="width: 2%; vertical-align: top;">&#160;</td>
        <td style="width: 49%; vertical-align: top;">
          <div style="text-align: center; color: #000000; font-weight: bold;">(I.R.S. Employer</div>
          <div style="text-align: center; color: #000000; font-weight: bold;">Identification No.)</div>
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        <td style="width: 49%; vertical-align: middle;">&#160;</td>
        <td style="width: 2%; vertical-align: middle;">&#160;</td>
        <td style="width: 49%; vertical-align: middle;">&#160;</td>
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        <td style="width: 49%; vertical-align: top;">
          <div style="text-align: center; color: #000000; font-weight: bold;">9540 Towne Centre Drive, Suite 100</div>
          <div style="text-align: center; color: #000000; font-weight: bold;">San Diego, California</div>
        </td>
        <td style="width: 2%; vertical-align: top;">&#160;</td>
        <td style="width: 49%; vertical-align: bottom;">
          <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">92121</div>
        </td>
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        <td style="width: 49%; vertical-align: top;">
          <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">(Address of Principal Executive Offices)</div>
        </td>
        <td style="width: 2%; vertical-align: top;">&#160;</td>
        <td style="width: 49%; vertical-align: top;">
          <div style="text-align: center; color: #000000; font-weight: bold;">(Zip Code)</div>
        </td>
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  <div style="text-align: center; color: #000000; font-weight: bold;"> <br>
  </div>
  <div style="text-align: center; color: #000000; font-weight: bold;">Bionano Genomics, Inc. Amended and Restated 2018 Equity Incentive Plan, as amended</div>
  <div style="text-align: center; color: #000000; font-weight: bold;">Bionano Genomics, Inc. 2018 Employee Stock Purchase Plan</div>
  <div style="text-align: center; color: #000000; font-weight: bold;">Bionano Genomics, Inc. 2020 Inducement Plan, as amended</div>
  <div style="text-align: center; color: #000000; font-weight: bold;">(Full titles of the plans)</div>
  <div><br>
  </div>
  <div style="text-align: center; color: #000000; font-weight: bold;">R. Erik Holmlin, Ph.D.</div>
  <div style="text-align: center; color: #000000; font-weight: bold;">President and Chief Executive Officer</div>
  <div style="text-align: center; color: #000000; font-weight: bold;">Bionano Genomics, Inc.</div>
  <div style="text-align: center; color: #000000; font-weight: bold;">9540 Towne Centre Drive, Suite 100</div>
  <div style="text-align: center; color: #000000; font-weight: bold;">San Diego, California 92121</div>
  <div style="text-align: center; color: #000000; font-weight: bold;">(858) 888-7600</div>
  <div style="text-align: center; color: #000000; font-weight: bold;">(Name, address, including zip code, and telephone number, including area code, of agent for service)</div>
  <div><br>
  </div>
  <div style="text-align: center; color: #000000; font-style: italic; font-weight: bold;">Copies to:</div>
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          <div style="text-align: center; color: #000000; font-weight: bold;">Thomas A. Coll, Esq.</div>
          <div style="text-align: center; color: #000000; font-weight: bold;">Su Lian Lu, Esq.</div>
          <div style="text-align: center; color: #000000; font-weight: bold;">Cooley LLP</div>
          <div style="text-align: center; color: #000000; font-weight: bold;">10265 Science Center Drive</div>
          <div style="text-align: center; color: #000000; font-weight: bold;">San Diego, California 92121</div>
          <div style="text-align: center; color: #000000; font-weight: bold;">(858) 550-6000</div>
        </td>
        <td style="width: 2%; vertical-align: top;">&#160;</td>
        <td style="width: 49%; vertical-align: top;">
          <div style="text-align: center; color: #000000; font-weight: bold;">R. Erik Holmlin, Ph.D.</div>
          <div style="text-align: center; color: #000000; font-weight: bold;">President and Chief Executive Officer</div>
          <div style="text-align: center; color: #000000; font-weight: bold;">Bionano Genomics, Inc.</div>
          <div style="text-align: center; color: #000000; font-weight: bold;">9540 Towne Centre Drive, Suite 100</div>
          <div style="text-align: center; color: #000000; font-weight: bold;">San Diego, California 92121</div>
          <div style="text-align: center; color: #000000; font-weight: bold;">(858) 888-7600</div>
        </td>
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  <div style="text-align: justify; color: #000000;"> <br>
  </div>
  <div style="text-align: justify; color: #000000;">Indicate by check mark whether the Registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, a smaller reporting company or an emerging growth company. See the definitions of
    &#8220;large accelerated filer,&#8221; &#8220;accelerated filer,&#8221; &#8220;smaller reporting company,&#8221; and &#8220;emerging growth company&#8221; in Rule 12b-2 of the Exchange Act.</div>
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        <td style="width: 43.04%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">Large accelerated filer</div>
        </td>
        <td style="width: 6.95%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">&#9744;</div>
        </td>
        <td style="width: 43.04%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">Accelerated filer</div>
        </td>
        <td style="width: 6.95%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">&#9744;</div>
        </td>
      </tr>
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        <td style="width: 43.04%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">Non-accelerated filer</div>
        </td>
        <td style="width: 6.95%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">&#9746;</div>
        </td>
        <td style="width: 43.04%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">Smaller reporting company</div>
        </td>
        <td style="width: 6.95%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">&#9746;</div>
        </td>
      </tr>
      <tr>
        <td style="width: 43.04%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">Emerging growth company</div>
        </td>
        <td style="width: 6.95%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">&#9744;</div>
        </td>
        <td style="width: 43.04%; vertical-align: top;">&#160;</td>
        <td style="width: 6.95%; vertical-align: top;">&#160;</td>
      </tr>

  </table>
  <div style="text-align: justify; color: #000000;"> <br>
  </div>
  <div style="text-align: justify; color: #000000;">If an emerging growth company, indicate by check mark if the Registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards provided
    pursuant to Section 7(a)(2)(B) of the Securities Act. &#9744;</div>
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  <div style="text-align: center; color: #000000; font-weight: bold;">EXPLANATORY NOTE</div>
  <div><br>
  </div>
  <div style="text-align: justify;"><font style="color: #000000;">This Registration Statement on Form S-8 is being filed by Bionano Genomics, Inc. (the &#8220;</font><font style="font-weight: bold; font-style: italic; color: #000000;">Registrant</font><font style="color: #000000;">&#8221;) </font>with the Securities and Exchange Commission (the &#8220;<font style="font-weight: bold; font-style: italic;">SEC</font>&#8221;) <font style="color: rgb(0, 0, 0);">for the purpose of registering (i) an additional 93,270</font>&#160;<font style="color: rgb(0, 0, 0);">shares of Registrant&#8217;s Common Stock, par value $0.0001 per share (&#8220;</font><font style="font-weight: bold; font-style: italic; color: rgb(0, 0, 0);">Common Stock</font><font style="color: rgb(0, 0, 0);">&#8221;), issuable under
      the Registrant&#8217;s 2018 Equity Incentive Plan, as amended (the &#8220;</font><font style="font-weight: bold; font-style: italic; color: rgb(0, 0, 0);">2018 Plan</font><font style="color: rgb(0, 0, 0);">&#8221;), pursuant to the provisions of the 2018 Plan
      providing for an automatic increase on January 1 of each year in the number of shares of Common Stock reserved and available for issuance under the 2018 Plan, (ii) an additional 366</font> shares of Common Stock <font style="color: rgb(0, 0, 0);">issuable




    </font>under the Registrant&#8217;s 2018 Employee Stock Purchase Plan (the &#8220;<font style="font-weight: bold; font-style: italic;">2018 ESPP</font>&#8221;) pursuant to the provisions of the 2018 ESPP providing for an automatic increase <font style="color: rgb(0, 0, 0);">on January 1 of each year </font>in the number of shares of Common Stock reserved and available for issuance under the 2018 ESPP, and (iii) an additional 30,000 shares of Common Stock issuable under the Registrant&#8217;s 2020 Inducement Plan (the &#8220;<font style="font-weight: bold; font-style: italic;">Inducement Plan</font>&#8221;), for an aggregate of 36,833 shares of Common Stock issuable under the Inducement Plan, pursuant to an amendment to the Inducement Plan approved by the Board of Directors of the
    Registrant on March 7, 2025. In accordance with the instructional note to Part I of Form S-8 as promulgated by the SEC, the information specified by Part I of the Form S-8 has been omitted from this Registration Statement.</div>
  <div><br>
  </div>
  <div style="text-align: center; color: #000000; font-weight: bold;">PART II</div>
  <div><br>
  </div>
  <div style="text-align: center; margin-right: 6.7pt; margin-left: 4.8pt; font-weight: bold;">INFORMATION REQUIRED IN THE REGISTRATION STATEMENT</div>
  <div>&#160;</div>
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        <td style="width: 40pt; vertical-align: top; font-weight: bold;">Item 3.</td>
        <td style="width: auto; vertical-align: top;">
          <div style="font-weight: bold;">Incorporation of Documents by Reference.</div>
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  <div><br>
  </div>
  <div style="text-align: justify;"><font style="color: #000000;">These additional shares of Common Stock are securities of the same class as other securities for which a Registration Statement of the </font>Registrant on Form S-8 relating to the 2018
    Plan, the 2018 ESPP, and the Inducement Plan are effective. The Registrant previously registered shares of its Common Stock for issuance under the 2018 Plan, the 2018 ESPP, and the Inducement Plan under Registration Statements on Form S-8 as noted
    below.</div>
  <div><br>
  </div>
  <div style="text-align: justify;">The following documents filed by the Registrant with the SEC<font style="font-weight: bold; font-style: italic;">&#160;</font>are incorporated by reference into this Registration Statement:</div>
  <div><br>
  </div>
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        <td style="width: 18pt;">&#160;</td>
        <td style="width: 18pt; vertical-align: top;">(a)</td>
        <td style="width: auto; vertical-align: top; text-align: justify;">
          <div>The contents of the earlier registration statements on Form S-8 pertaining to the 2018 Plan and 2018 ESPP filed with the SEC on <a href="https://www.sec.gov/Archives/edgar/data/1411690/000119312518260602/d616702ds8.htm">August 28, 2018</a>
            (File No. 333-227073), <a href="https://www.sec.gov/Archives/edgar/data/1411690/000119312519091060/d626460ds8.htm">March 29, 2019</a> (File No. 333-230589), <a href="https://www.sec.gov/Archives/edgar/data/1411690/000114036120005371/nt10009730x2_s8.htm">March 11, 2020</a> (File No. 333-237069), <a href="https://www.sec.gov/Archives/edgar/data/1411690/000114036120018366/nt10014149x1_s8.htm">August
              14, 2020</a> (File No. 333-245764), <a href="https://www.sec.gov/Archives/edgar/data/1411690/000114036121009780/nt10022040x2_s8.htm">March 24, 2021</a> (File No. 333-254654), <a href="https://www.sec.gov/Archives/edgar/data/1411690/000114036122007373/brhc10034627_s8.htm">March 1, 2022</a> (File No. 333-263176), <a href="https://www.sec.gov/Archives/edgar/data/1411690/000114036123010941/brhc10049419_s8.htm">March 9,
              2023</a> (File No. 333-270430), and <a href="https://www.sec.gov/Archives/edgar/data/1411690/000114036124024994/ef20023291_s8.htm">May 8, 2024</a> (File No. 333-279229);</div>
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        <td style="width: 18pt; vertical-align: top; color: rgb(0, 0, 0);">(b)</td>
        <td style="width: auto; vertical-align: top; text-align: justify;">
          <div>The contents of the earlier registration statements on Form S-8 pertaining to the Inducement Plan filed with the SEC on <a href="https://www.sec.gov/Archives/edgar/data/1411690/000114036120019327/nt10014149x5_s8.htm">August 28, 2020</a>
            (File No. 333-248468), <a href="https://www.sec.gov/Archives/edgar/data/1411690/000114036121036566/brhc10030312_s8.htm">November 4, 2021</a> (File No. 333-260762), and <a href="https://www.sec.gov/Archives/edgar/data/1411690/000114036123010941/brhc10049419_s8.htm">March 9, 2023</a> (File No. 333-270430);</div>
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  <div>&#160;</div>
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        <td style="width: 18pt; vertical-align: top; color: rgb(0, 0, 0);">(c)</td>
        <td style="width: auto; vertical-align: top; text-align: justify;">
          <div style="color: rgb(0, 0, 0);">The Registrant&#8217;s Annual Report on Form 10-K for the year ended December 31, 2024, which included audited financial statements for the Registrant&#8217;s latest fiscal year, filed with the SEC on <a href="https://www.sec.gov/ix?doc=/Archives/edgar/data/1411690/000141169025000037/bngo-20241231.htm">March 31, 2025</a>;</div>
        </td>
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  <div><br>
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        <td style="width: 18pt; vertical-align: top; color: rgb(0, 0, 0);">(d)</td>
        <td style="width: auto; vertical-align: top; text-align: justify;">
          <div style="color: rgb(0, 0, 0);">The Registrant's Current Reports on Form 8-K filed with the SEC on <a href="https://www.sec.gov/ix?doc=/Archives/edgar/data/1411690/000114036125000296/ny20041129x2_8k.htm">January 6, 2025</a> (with respect to
            Items 1.01 and 8.01 only), <a href="https://www.sec.gov/ix?doc=/Archives/edgar/data/1411690/000141169025000003/bngo-20250113.htm">January 13, 2025</a>, <a href="https://www.sec.gov/ix?doc=/Archives/edgar/data/1411690/000141169025000005/bngo-20250115.htm">January 16, 2025</a>, <a href="https://www.sec.gov/ix?doc=/Archives/edgar/data/1411690/000141169025000008/bngo-20250124.htm">January 27, 2025</a>
            (with respect to Items 3.03 and 5.03 only), <a href="https://www.sec.gov/ix?doc=/Archives/edgar/data/1411690/000114036125003762/ef20043253_8k.htm">February 10, 2025</a> and <a href="https://www.sec.gov/ix?doc=/Archives/edgar/data/1411690/000114036125005427/ef20043949_8k.htm">February 21, 2025</a> (with respect to Item 1.01 only); and</div>
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        <td style="width: 18pt; vertical-align: top; color: rgb(0, 0, 0);">(e)</td>
        <td style="width: auto; vertical-align: top; text-align: justify;">
          <div><font style="color: #000000;">The description of the Registrant&#8217;s Common Stock contained in a registration statement on Form 8-A filed with the SEC on </font><a href="https://www.sec.gov/Archives/edgar/data/1411690/000119312518233217/d571624d8a12b.htm">July 31, 2018</a><font style="color: rgb(0, 0, 0);"> (File No. 001-38613) under the Securities Exchange Act of 1934, as amended (the &#8220;</font><font style="font-weight: bold; font-style: italic; color: rgb(0, 0, 0);">Exchange Act</font><font style="color: rgb(0, 0, 0);">&#8221;), including any amendment or report filed for the purpose of updating such description including <a href="https://www.sec.gov/Archives/edgar/data/1411690/000141169024000013/bngo-ex45x20231231.htm">Exhibit 4.5</a> of the Company&#8217;s Annual Report on Form 10-K for the year ended December 31, 2023, filed with the SEC on </font><a href="https://www.sec.gov/ix?doc=/Archives/edgar/data/0001411690/000141169024000013/bngo-20231231.htm">March 5, 2024</a><font style="color: rgb(0, 0, 0);">.</font></div>
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  <div><br>
  </div>
  <div style="text-align: justify; color: #000000;">All reports and other documents subsequently filed by the Registrant pursuant to Section 13(a), 13(c), 14 or 15(d) of the Securities Exchange Act of 1934, as amended, other than Current Reports or
    portions thereof furnished under Item 2.02 or Item 7.01 or any related Item 9.01 of Form 8-K and exhibits furnished on such form that relate to such items and other portions of documents that are furnished but not filed or are otherwise not
    incorporated into registration statements pursuant to the applicable rules promulgated by the SEC, after the date of this registration statement and prior to the filing of a post-effective amendment which indicates that all securities offered hereby
    have been sold or which deregisters all securities offered hereby then remaining unsold, shall be deemed to be incorporated by reference herein and to be a part of this registration statement from the date of filing such reports and documents.</div>
  <div><br>
  </div>
  <div style="text-align: justify; color: #000000;">Any statement contained in a document incorporated or deemed to be incorporated herein by reference shall be deemed to be modified or superseded for purposes of this registration statement to the extent
    that a statement herein or in any other subsequently filed document which also is or is deemed to be incorporated by reference herein modifies or supersedes that statement. Any such statement so modified or superseded shall not constitute a part of
    this registration statement, except as so modified or superseded.</div>
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      <tr>
        <td style="width: 40pt; vertical-align: top; color: rgb(0, 0, 0); font-weight: bold;">Item 8.</td>
        <td style="width: auto; vertical-align: top;">
          <div style="color: rgb(0, 0, 0); font-weight: bold;">Exhibits.</div>
        </td>
      </tr>

  </table>
  <div><br>
  </div>
  <table cellspacing="0" cellpadding="0" border="0" style="font-family: 'Times New Roman'; font-size: 10pt; width: 100%; border-collapse: collapse; text-align: left; color: #000000;" id="z75a36f2dbb6b473f80f9fd5f20ef7164">

      <tr>
        <td style="width: 8%; vertical-align: bottom; border-bottom: 2px solid rgb(0, 0, 0);">
          <div style="color: #000000; font-weight: bold;">Exhibit</div>
          <div style="color: #000000; font-weight: bold;">Number</div>
        </td>
        <td style="width: 2%; vertical-align: top;">&#160;</td>
        <td style="width: 90%; vertical-align: bottom; border-bottom: 2px solid rgb(0, 0, 0);">
          <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">Description</div>
        </td>
      </tr>
      <tr>
        <td style="width: 8%; vertical-align: top; background-color: rgb(204, 238, 255);">
          <div>4.1<sup style="vertical-align: text-top; line-height: 1; font-size: smaller;">(1)</sup></div>
        </td>
        <td style="width: 2%; vertical-align: top; background-color: rgb(204, 238, 255);">&#160;</td>
        <td style="width: 90%; vertical-align: top; background-color: rgb(204, 238, 255);">
          <div><a href="https://www.sec.gov/Archives/edgar/data/1411690/000141169023000082/bngo-2023x09x30xexx31.htm">Amended and Restated Certificate of Incorporation of the Registrant, as amended<font style="color: rgb(0, 0, 0);">.</font></a></div>
        </td>
      </tr>
      <tr>
        <td style="width: 8%; vertical-align: top;">
          <div>4.2<sup style="vertical-align: text-top; line-height: 1; font-size: smaller;">(2)</sup></div>
        </td>
        <td style="width: 2%; vertical-align: top;">&#160;</td>
        <td style="width: 90%; vertical-align: top;">
          <div><a href="https://www.sec.gov/Archives/edgar/data/1411690/000141169025000008/bionano-certificateofamend.htm">Certificate of Amendment to the Amended and Restated Certificate of Incorporation, as amended.</a></div>
        </td>
      </tr>
      <tr>
        <td style="width: 8%; vertical-align: top; background-color: rgb(204, 238, 255);">
          <div>4.3<sup style="vertical-align: text-top; line-height: 1; font-size: smaller;">(3)</sup></div>
        </td>
        <td style="width: 2%; vertical-align: top; background-color: rgb(204, 238, 255);">&#160;</td>
        <td style="width: 90%; vertical-align: top; background-color: rgb(204, 238, 255);">
          <div><a href="https://www.sec.gov/Archives/edgar/data/1411690/000119312518257763/d607608dex32.htm">Amended and Restated Bylaws of the Registrant<font style="color: rgb(0, 0, 0);">.</font></a></div>
        </td>
      </tr>
      <tr>
        <td style="width: 8%; vertical-align: top;">
          <div>4.4<sup style="vertical-align: text-top; line-height: 1; font-size: smaller;">(4)</sup></div>
        </td>
        <td style="width: 2%; vertical-align: top;">&#160;</td>
        <td style="width: 90%; vertical-align: top;">
          <div><a href="https://www.sec.gov/Archives/edgar/data/1411690/000114036123018454/brhc20051350_ex3-2.htm">Amendment to Amended and Restated Bylaws.</a></div>
        </td>
      </tr>
      <tr>
        <td style="width: 8%; vertical-align: top; background-color: rgb(204, 238, 255);">
          <div>4.5<sup style="vertical-align: text-top; line-height: 1; font-size: smaller;">(5)</sup></div>
        </td>
        <td style="width: 2%; vertical-align: top; background-color: rgb(204, 238, 255);">&#160;</td>
        <td style="width: 90%; vertical-align: top; background-color: rgb(204, 238, 255);">
          <div><a href="https://www.sec.gov/Archives/edgar/data/1411690/000141169024000013/bngo-ex41x20231231.htm">Form of Common Stock Certificate of the Registrant<font style="color: rgb(0, 0, 0);">.</font></a></div>
        </td>
      </tr>
      <tr>
        <td style="width: 8%; vertical-align: top;">
          <div>5.1*</div>
        </td>
        <td style="width: 2%; vertical-align: top;">&#160;</td>
        <td style="width: 90%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);"><a href="ef20046763_ex5-1.htm">Opinion of Cooley LLP.</a></div>
        </td>
      </tr>
      <tr>
        <td style="width: 8%; vertical-align: top; background-color: rgb(204, 238, 255);">
          <div>23.1*</div>
        </td>
        <td style="width: 2%; vertical-align: top; background-color: rgb(204, 238, 255);">&#160;</td>
        <td style="width: 90%; vertical-align: top; background-color: rgb(204, 238, 255);">
          <div style="color: rgb(0, 0, 0);"><a href="ef20046763_ex23-1.htm">Consent of BDO USA, P.C., Independent Registered Public Accounting Firm.</a></div>
        </td>
      </tr>
      <tr>
        <td style="width: 8%; vertical-align: top;">
          <div>23.2*</div>
        </td>
        <td style="width: 2%; vertical-align: top;">&#160;</td>
        <td style="width: 90%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);"><a href="ef20046763_ex5-1.htm">Consent of Cooley LLP. Reference is made to Exhibit 5.1.</a></div>
        </td>
      </tr>
      <tr>
        <td style="width: 8%; vertical-align: top; background-color: rgb(204, 238, 255);">
          <div>24.1*</div>
        </td>
        <td style="width: 2%; vertical-align: top; background-color: rgb(204, 238, 255);">&#160;</td>
        <td style="width: 90%; vertical-align: top; background-color: rgb(204, 238, 255);">
          <div style="color: rgb(0, 0, 0);"><a href="#POWEROFATTORNEY">Power of Attorney. Reference is made to the signature page hereto.</a></div>
        </td>
      </tr>
      <tr>
        <td style="width: 8%; vertical-align: top;">
          <div>99.1<sup style="vertical-align: text-top; line-height: 1; font-size: smaller;">(6)</sup></div>
        </td>
        <td style="width: 2%; vertical-align: top;">&#160;</td>
        <td style="width: 90%; vertical-align: top;">
          <div><a href="https://www.sec.gov/Archives/edgar/data/1411690/000114036120018366/nt10014149x1_ex99-1.htm">Bionano Genomics, Inc. 2018 Equity Incentive Plan, as amended<font style="color: rgb(0, 0, 0);">.</font></a></div>
        </td>
      </tr>
      <tr>
        <td style="width: 8%; vertical-align: top; background-color: rgb(204, 238, 255);">
          <div>99.2<sup style="vertical-align: text-top; line-height: 1; font-size: smaller;">(7)</sup></div>
        </td>
        <td style="width: 2%; vertical-align: top; background-color: rgb(204, 238, 255);">&#160;</td>
        <td style="width: 90%; vertical-align: top; background-color: rgb(204, 238, 255);">
          <div><a href="https://www.sec.gov/Archives/edgar/data/1411690/000119312518260602/d616702dex995.htm">Bionano Genomics, Inc. 2018 Employee Stock Purchase Plan<font style="color: rgb(0, 0, 0);">.</font></a></div>
        </td>
      </tr>
      <tr>
        <td style="width: 8%; vertical-align: top;">
          <div>99.3*</div>
        </td>
        <td style="width: 2%; vertical-align: top;">&#160;</td>
        <td style="width: 90%; vertical-align: top;">
          <div><a href="ef20046763_ex99-3.htm">Bionano Genomics, Inc. 2020 Inducement Plan, as amended.</a></div>
        </td>
      </tr>
      <tr>
        <td style="width: 8%; vertical-align: top; background-color: rgb(204, 238, 255);">
          <div>107*</div>
        </td>
        <td style="width: 2%; vertical-align: top; background-color: rgb(204, 238, 255);">&#160;</td>
        <td style="width: 90%; vertical-align: top; background-color: rgb(204, 238, 255);">
          <div style="color: rgb(0, 0, 0);"><a href="ef20046763_ex107.htm">Filing Fee Table.</a></div>
        </td>
      </tr>

  </table>
  <div><br>
  </div>
  <table cellspacing="0" cellpadding="0" border="0" style="font-family: 'Times New Roman'; font-size: 10pt; width: 100%; border-collapse: collapse; text-align: left; color: #000000;">

      <tr>
        <td style="width: 5%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">(1)</div>
        </td>
        <td style="width: 95%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">Incorporated by reference to Exhibit 3.1 of the Registrant&#8217;s Quarterly Report on Form 10-Q, filed with the SEC on November 8, 2023.</div>
        </td>
      </tr>
      <tr>
        <td style="width: 5%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">(2)</div>
        </td>
        <td style="width: 95%; vertical-align: top;">
          <div>Incorporated by reference to Exhibit 3.1 to Registrant&#8217;s Current Report on Form 8-K, filed with the SEC on January 27, 2025<font style="color: rgb(0, 0, 0);">.</font></div>
        </td>
      </tr>
      <tr>
        <td style="width: 5%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">(3)</div>
        </td>
        <td style="width: 95%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">Incorporated by reference to Exhibit 3.2 of the Registrant&#8217;s Current Report on Form 8-K, filed with the SEC on August 24, 2018.</div>
        </td>
      </tr>
      <tr>
        <td style="width: 5%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">(4)</div>
        </td>
        <td style="width: 95%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">Incorporated by reference to Exhibit 3.2 of the Registrant&#8217;s Current Report on Form 8-K, filed with the SEC on April 14, 2023.</div>
        </td>
      </tr>
      <tr>
        <td style="width: 5%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">(5)</div>
        </td>
        <td style="width: 95%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">Incorporated by reference to Exhibit 4.1 of the Registrant&#8217;s Annual Report on Form 10-K, filed with the SEC on March 5, 2024.</div>
        </td>
      </tr>
      <tr>
        <td style="width: 5%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">(6)</div>
        </td>
        <td style="width: 95%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">Incorporated by reference to Exhibit 99.1 of the Registrant&#8217;s Registration Statement on Form S-8 (File No. 333-245764), filed with the SEC on August 13, 2020.</div>
        </td>
      </tr>
      <tr>
        <td style="width: 5%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">(7)</div>
        </td>
        <td style="width: 95%; vertical-align: top;">
          <div style="color: rgb(0, 0, 0);">Incorporated by reference to Exhibit 99.5 of the Registrant&#8217;s Registration Statement on Form S-8 (File No. 333-227073), filed with the SEC on August 28, 2018.</div>
        </td>
      </tr>
      <tr>
        <td style="width: 5%; vertical-align: top;" rowspan="1">&#160;</td>
        <td style="width: 95%; vertical-align: top;" rowspan="1">&#160;</td>
      </tr>
      <tr>
        <td style="width: 5%; vertical-align: top;" rowspan="1">
          <div>*</div>
        </td>
        <td style="width: 95%; vertical-align: top;" rowspan="1">
          <div>Filed herewith.</div>
        </td>
      </tr>

  </table>
  <div><br>
  </div>
  <div style="clear: both; margin-top: 10pt; margin-bottom: 10pt;" class="BRPFPageBreakArea">
    <div style="page-break-after: always;" class="BRPFPageBreak">
      <hr noshade="noshade" style="margin: 4px 0px; width: 100%; border-width: 0; height: 2px; color: #000000; background-color: #000000; clear: both;"></div>
  </div>
  <div style="text-align: center; color: #000000; font-weight: bold;">SIGNATURES</div>
  <div><br>
  </div>
  <div style="text-align: justify; color: #000000;">Pursuant to the requirements of the Securities Act of 1933, as amended, the Registrant certifies that it has reasonable grounds to believe that it meets all of the requirements for filing on Form S-8 and
    has duly caused this Registration Statement to be signed on its behalf by the undersigned, thereunto duly authorized, in the City of San Diego, State of California, on April 1, 2025.</div>
  <div><br>
  </div>
  <table cellspacing="0" cellpadding="0" border="0" style="font-family: 'Times New Roman'; font-size: 10pt; width: 100%; border-collapse: collapse; text-align: left; color: #000000;" id="z759808cb0bae4cba8ca6272eab8f2568">

      <tr>
        <td colspan="2" style="vertical-align: top;">
          <div style="text-indent: -10pt; margin-left: 10pt; color: rgb(0, 0, 0); font-weight: bold;">BIONANO GENOMICS, INC.</div>
        </td>
        <td style="width: 50%; vertical-align: top;">&#160;</td>
      </tr>
      <tr>
        <td style="width: 3%; vertical-align: middle;">&#160;</td>
        <td style="width: 47%; vertical-align: middle;">&#160;</td>
        <td style="width: 50%; vertical-align: top;">&#160;</td>
      </tr>
      <tr>
        <td style="width: 3%; vertical-align: top;">
          <div style="text-indent: -10pt; margin-left: 10pt; color: rgb(0, 0, 0);">By:</div>
        </td>
        <td style="width: 47%; vertical-align: bottom; border-bottom: 2px solid rgb(0, 0, 0);">
          <div style="text-indent: -10pt; margin-left: 10pt; color: rgb(0, 0, 0);">/s/ R. Erik Holmlin, Ph.D.</div>
        </td>
        <td style="width: 50%; vertical-align: top;">&#160;</td>
      </tr>
      <tr>
        <td style="width: 3%; vertical-align: top;">&#160;</td>
        <td style="width: 47%; vertical-align: bottom;">
          <div style="text-indent: -10pt; margin-left: 10pt; color: rgb(0, 0, 0);">R. Erik Holmlin, Ph.D.</div>
        </td>
        <td style="width: 50%; vertical-align: top;">&#160;</td>
      </tr>
      <tr>
        <td style="width: 3%; vertical-align: top;">&#160;</td>
        <td style="width: 47%; vertical-align: bottom;">
          <div style="text-indent: -10pt; margin-left: 10pt; color: #000000; font-style: italic;">President and Chief Executive Officer</div>
          <div>&#160;</div>
        </td>
        <td style="width: 50%; vertical-align: top;">&#160;</td>
      </tr>

  </table>
  <div><br>
  </div>
  <a name="POWEROFATTORNEY"><!--Anchor--></a>
  <div style="text-align: center; color: #000000; font-weight: bold;">POWER OF ATTORNEY</div>
  <div><br>
  </div>
  <div style="text-align: justify; color: #000000;">KNOW ALL BY THESE PRESENTS, that each person whose signature appears below constitutes and appoints each of R. Erik Holmlin, Ph.D. and Mark Adamchak as their true and lawful attorney-in-fact and agent,
    with the full power of substitution, for him or her and in his or her name, place or stead, in any and all capacities, to sign any and all amendments to this Registration Statement (including post-effective amendments), and to file the same, with
    exhibits thereto and other documents in connection therewith, with the Securities and Exchange Commission, granting unto said attorney-in-fact and agent full power and authority to do and perform each and every act and thing requisite and necessary to
    be done in and about the premises, as fully to all intents and purposes as he or she might or could do in person, hereby ratifying and confirming all that said attorney-in-fact and agent, or his substitute or substitutes, may lawfully do or cause to be
    done by virtue hereof.</div>
  <div><br>
  </div>
  <div style="text-align: justify; color: #000000;">Pursuant to the requirements of the Securities Act of 1933, as amended, this Registration Statement has been signed by the following persons in the capacities and on the dates indicated.</div>
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  <table cellspacing="0" cellpadding="0" border="0" style="font-family: 'Times New Roman'; font-size: 10pt; width: 100%; border-collapse: collapse; text-align: left; color: #000000;" id="zada27c45a1ba4b018d2984906e505e36">

      <tr>
        <td style="width: 38%; vertical-align: bottom; border-bottom: 2px solid rgb(0, 0, 0);">
          <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">Signature</div>
        </td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 38%; vertical-align: bottom; border-bottom: 2px solid rgb(0, 0, 0);">
          <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">Title</div>
        </td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 20.45%; vertical-align: bottom; border-bottom: 2px solid rgb(0, 0, 0);">
          <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">Date</div>
        </td>
      </tr>
      <tr>
        <td style="width: 38%; vertical-align: bottom;">&#160;</td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 38%; vertical-align: bottom;">&#160;</td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 20.45%; vertical-align: bottom;">&#160;</td>
      </tr>
      <tr>
        <td style="width: 38%; vertical-align: bottom; border-bottom: 2px solid rgb(0, 0, 0);">
          <div style="text-align: center; color: rgb(0, 0, 0);">/s/ R. Erik Holmlin, Ph.D.</div>
        </td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td rowspan="2" style="width: 38%; vertical-align: middle;">
          <div style="text-align: center;">President, Chief Executive Officer and Director</div>
          <div style="text-align: center; font-style: italic;">(Principal Executive Officer and Principal Financial Officer)</div>
        </td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 20.45%; vertical-align: bottom;">
          <div style="text-align: center; color: rgb(0, 0, 0);">April 1, 2025</div>
        </td>
      </tr>
      <tr>
        <td style="width: 38%; vertical-align: middle;">
          <div style="text-align: center; color: rgb(0, 0, 0);">R. Erik Holmlin, Ph.D.</div>
        </td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 20.45%; vertical-align: bottom;">&#160;</td>
      </tr>
      <tr>
        <td style="width: 38%; vertical-align: bottom;">&#160;</td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 38%; vertical-align: bottom;">&#160;</td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 20.45%; vertical-align: bottom;">&#160;</td>
      </tr>
      <tr>
        <td style="width: 38%; vertical-align: bottom; border-bottom: 2px solid rgb(0, 0, 0);">
          <div style="text-align: center; color: rgb(0, 0, 0);">/s/ Mark Adamchak</div>
        </td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td rowspan="2" style="width: 38%; vertical-align: middle;">
          <div style="text-align: center;">VP, Accounting<br>
          </div>
          <div style="text-align: center; font-style: italic;">(Principal Accounting Officer)</div>
        </td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 20.45%; vertical-align: bottom;">
          <div style="text-align: center; color: rgb(0, 0, 0);">April 1, 2025</div>
        </td>
      </tr>
      <tr>
        <td style="width: 38%; vertical-align: bottom;">
          <div style="text-align: center; color: rgb(0, 0, 0);">Mark Adamchak</div>
        </td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 20.45%; vertical-align: bottom;">&#160;</td>
      </tr>
      <tr>
        <td style="width: 38%; vertical-align: bottom;">&#160;</td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 38%; vertical-align: bottom;">&#160;</td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 20.45%; vertical-align: bottom;">&#160;</td>
      </tr>
      <tr>
        <td style="width: 38%; vertical-align: bottom; border-bottom: 2px solid rgb(0, 0, 0);">
          <div style="text-align: center; color: rgb(0, 0, 0);">/s/ David L. Barker, Ph.D.</div>
        </td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td rowspan="2" style="width: 38%; vertical-align: middle;">
          <div style="text-align: center; color: rgb(0, 0, 0);">Director</div>
        </td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 20.45%; vertical-align: bottom;">
          <div style="text-align: center; color: rgb(0, 0, 0);">April 1, 2025</div>
        </td>
      </tr>
      <tr>
        <td style="width: 38%; vertical-align: bottom;">
          <div style="text-align: center; color: rgb(0, 0, 0);">David L. Barker, Ph.D.</div>
        </td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 20.45%; vertical-align: bottom;">&#160;</td>
      </tr>
      <tr>
        <td style="width: 38%; vertical-align: bottom;">&#160;</td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 38%; vertical-align: bottom;">&#160;</td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 20.45%; vertical-align: bottom;">&#160;</td>
      </tr>
      <tr>
        <td style="width: 38%; vertical-align: bottom; border-bottom: 2px solid rgb(0, 0, 0);">
          <div style="text-align: center; color: rgb(0, 0, 0);">/s/ Yvonne Linney, Ph.D.</div>
        </td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td rowspan="2" style="width: 38%; vertical-align: middle;">
          <div style="text-align: center; color: rgb(0, 0, 0);">Director</div>
        </td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 20.45%; vertical-align: bottom;">
          <div style="text-align: center; color: rgb(0, 0, 0);">April 1, 2025</div>
        </td>
      </tr>
      <tr>
        <td style="width: 38%; vertical-align: bottom;">
          <div style="text-align: center; color: rgb(0, 0, 0);">Yvonne Linney, Ph.D.</div>
        </td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 20.45%; vertical-align: bottom;">&#160;</td>
      </tr>
      <tr>
        <td style="width: 38%; vertical-align: bottom;">&#160;</td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 38%; vertical-align: bottom;">&#160;</td>
        <td style="width: 2%; vertical-align: bottom;">&#160;</td>
        <td style="width: 20.45%; vertical-align: bottom;">&#160;</td>
      </tr>
      <tr>
        <td style="width: 38%; vertical-align: bottom; border-bottom: 2px solid rgb(0, 0, 0);">
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[{"type": "S-8", "sequence": "1", "filename": "ef20046763_s8.htm", "description": "S-8", "secsgml_size_bytes": 55712}]
{"company-data": {"conformed-name": "Bionano Genomics, Inc.", "cik": "0001411690", "assigned-sic": "3826", "organization-name": "08 Industrial Applications and Services", "ein": "261756290", "state-of-incorporation": "DE", "fiscal-year-end": "1231"}, "filing-values": {"form-type": "S-8", "act": "33", "file-number": "333-286325", "film-number": "25801874"}, "business-address": {"street1": "9540 TOWNE CENTRE DRIVE", "street2": "SUITE 100", "city": "SAN DIEGO", "state": "CA", "zip": "92121", "phone": "(858) 888-7600"}, "mail-address": {"street1": "9540 TOWNE CENTRE DRIVE", "street2": "SUITE 100", "city": "SAN DIEGO", "state": "CA", "zip": "92121"}, "former-company": [{"former-conformed-name": "Bionano Genomics, Inc", "date-changed": "20190308"}, {"former-conformed-name": "BioNano Genomics, Inc", "date-changed": "20120703"}, {"former-conformed-name": "BioNanomatrix Inc", "date-changed": "20070906"}]}
As filed with the Securities and Exchange Commission on April 1, 2025
Registration No. 333-
UNITED STATES
SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549
FORM S-8
REGISTRATION STATEMENT
UNDER
THE SECURITIES ACT OF 1933
Bionano Genomics, Inc.
(Exact name of registrant as specified in its charter)
Delaware
26-1756290
(State or other jurisdiction of
incorporation or organization)
(I.R.S. Employer
Identification No.)
9540 Towne Centre Drive, Suite 100
San Diego, California
92121
(Address of Principal Executive Offices)
(Zip Code)
Bionano Genomics, Inc. Amended and Restated 2018 Equity Incentive Plan, as amended
Bionano Genomics, Inc. 2018 Employee Stock Purchase Plan
Bionano Genomics, Inc. 2020 Inducement Plan, as amended
(Full titles of the plans)
R. Erik Holmlin, Ph.D.
President and Chief Executive Officer
Bionano Genomics, Inc.
9540 Towne Centre Drive, Suite 100
San Diego, California 92121
(858) 888-7600
(Name, address, including zip code, and telephone number, including area code, of agent for service)
Copies to:
Thomas A. Coll, Esq.
Su Lian Lu, Esq.
Cooley LLP
10265 Science Center Drive
San Diego, California 92121
(858) 550-6000
R. Erik Holmlin, Ph.D.
President and Chief Executive Officer
Bionano Genomics, Inc.
9540 Towne Centre Drive, Suite 100
San Diego, California 92121
(858) 888-7600
Indicate by check mark whether the Registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, a smaller reporting company or an emerging growth company. See the definitions of
    "large accelerated filer," "accelerated filer," "smaller reporting company," and "emerging growth company" in Rule 12b-2 of the Exchange Act.
Large accelerated filer
☐
Accelerated filer
☐
Non-accelerated filer
☒
Smaller reporting company
☒
Emerging growth company
☐
If an emerging growth company, indicate by check mark if the Registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards provided
    pursuant to Section 7(a)(2)(B) of the Securities Act. ☐
EXPLANATORY NOTE
This Registration Statement on Form S-8 is being filed by Bionano Genomics, Inc. (the "
Registrant
")
with the Securities and Exchange Commission (the "
SEC
")
for the purpose of registering (i) an additional 93,270
shares of Registrant's Common Stock, par value $0.0001 per share ("
Common Stock
"), issuable under
      the Registrant's 2018 Equity Incentive Plan, as amended (the "
2018 Plan
"), pursuant to the provisions of the 2018 Plan
      providing for an automatic increase on January 1 of each year in the number of shares of Common Stock reserved and available for issuance under the 2018 Plan, (ii) an additional 366
shares of Common Stock
issuable
under the Registrant's 2018 Employee Stock Purchase Plan (the "
2018 ESPP
") pursuant to the provisions of the 2018 ESPP providing for an automatic increase
on January 1 of each year
in the number of shares of Common Stock reserved and available for issuance under the 2018 ESPP, and (iii) an additional 30,000 shares of Common Stock issuable under the Registrant's 2020 Inducement Plan (the "
Inducement Plan
"), for an aggregate of 36,833 shares of Common Stock issuable under the Inducement Plan, pursuant to an amendment to the Inducement Plan approved by the Board of Directors of the
    Registrant on March 7, 2025. In accordance with the instructional note to Part I of Form S-8 as promulgated by the SEC, the information specified by Part I of the Form S-8 has been omitted from this Registration Statement.
PART II
INFORMATION REQUIRED IN THE REGISTRATION STATEMENT
Item 3.
Incorporation of Documents by Reference.
These additional shares of Common Stock are securities of the same class as other securities for which a Registration Statement of the
Registrant on Form S-8 relating to the 2018
    Plan, the 2018 ESPP, and the Inducement Plan are effective. The Registrant previously registered shares of its Common Stock for issuance under the 2018 Plan, the 2018 ESPP, and the Inducement Plan under Registration Statements on Form S-8 as noted
    below.
The following documents filed by the Registrant with the SEC
are incorporated by reference into this Registration Statement:
(a)
The contents of the earlier registration statements on Form S-8 pertaining to the 2018 Plan and 2018 ESPP filed with the SEC on
August 28, 2018
(File No. 333-227073),
March 29, 2019
(File No. 333-230589),
March 11, 2020
(File No. 333-237069),
August
              14, 2020
(File No. 333-245764),
March 24, 2021
(File No. 333-254654),
March 1, 2022
(File No. 333-263176),
March 9,
              2023
(File No. 333-270430), and
May 8, 2024
(File No. 333-279229);
(b)
The contents of the earlier registration statements on Form S-8 pertaining to the Inducement Plan filed with the SEC on
August 28, 2020
(File No. 333-248468),
November 4, 2021
(File No. 333-260762), and
March 9, 2023
(File No. 333-270430);
(c)
The Registrant's Annual Report on Form 10-K for the year ended December 31, 2024, which included audited financial statements for the Registrant's latest fiscal year, filed with the SEC on
March 31, 2025
;
(d)
The Registrant's Current Reports on Form 8-K filed with the SEC on
January 6, 2025
(with respect to
            Items 1.01 and 8.01 only),
January 13, 2025
,
January 16, 2025
,
January 27, 2025
(with respect to Items 3.03 and 5.03 only),
February 10, 2025
and
February 21, 2025
(with respect to Item 1.01 only); and
(e)
The description of the Registrant's Common Stock contained in a registration statement on Form 8-A filed with the SEC on
July 31, 2018
(File No. 001-38613) under the Securities Exchange Act of 1934, as amended (the "
Exchange Act
"), including any amendment or report filed for the purpose of updating such description including
Exhibit 4.5
of the Company's Annual Report on Form 10-K for the year ended December 31, 2023, filed with the SEC on
March 5, 2024
.
All reports and other documents subsequently filed by the Registrant pursuant to Section 13(a), 13(c), 14 or 15(d) of the Securities Exchange Act of 1934, as amended, other than Current Reports or
    portions thereof furnished under Item 2.02 or Item 7.01 or any related Item 9.01 of Form 8-K and exhibits furnished on such form that relate to such items and other portions of documents that are furnished but not filed or are otherwise not
    incorporated into registration statements pursuant to the applicable rules promulgated by the SEC, after the date of this registration statement and prior to the filing of a post-effective amendment which indicates that all securities offered hereby
    have been sold or which deregisters all securities offered hereby then remaining unsold, shall be deemed to be incorporated by reference herein and to be a part of this registration statement from the date of filing such reports and documents.
Any statement contained in a document incorporated or deemed to be incorporated herein by reference shall be deemed to be modified or superseded for purposes of this registration statement to the extent
    that a statement herein or in any other subsequently filed document which also is or is deemed to be incorporated by reference herein modifies or supersedes that statement. Any such statement so modified or superseded shall not constitute a part of
    this registration statement, except as so modified or superseded.
Item 8.
Exhibits.
Exhibit
Number
Description
4.1
(1)
Amended and Restated Certificate of Incorporation of the Registrant, as amended
.
4.2
(2)
Certificate of Amendment to the Amended and Restated Certificate of Incorporation, as amended.
4.3
(3)
Amended and Restated Bylaws of the Registrant
.
4.4
(4)
Amendment to Amended and Restated Bylaws.
4.5
(5)
Form of Common Stock Certificate of the Registrant
.
5.1*
Opinion of Cooley LLP.
23.1*
Consent of BDO USA, P.C., Independent Registered Public Accounting Firm.
23.2*
Consent of Cooley LLP. Reference is made to Exhibit 5.1.
24.1*
Power of Attorney. Reference is made to the signature page hereto.
99.1
(6)
Bionano Genomics, Inc. 2018 Equity Incentive Plan, as amended
.
99.2
(7)
Bionano Genomics, Inc. 2018 Employee Stock Purchase Plan
.
99.3*
Bionano Genomics, Inc. 2020 Inducement Plan, as amended.
107*
Filing Fee Table.
(1)
Incorporated by reference to Exhibit 3.1 of the Registrant's Quarterly Report on Form 10-Q, filed with the SEC on November 8, 2023.
(2)
Incorporated by reference to Exhibit 3.1 to Registrant's Current Report on Form 8-K, filed with the SEC on January 27, 2025
.
(3)
Incorporated by reference to Exhibit 3.2 of the Registrant's Current Report on Form 8-K, filed with the SEC on August 24, 2018.
(4)
Incorporated by reference to Exhibit 3.2 of the Registrant's Current Report on Form 8-K, filed with the SEC on April 14, 2023.
(5)
Incorporated by reference to Exhibit 4.1 of the Registrant's Annual Report on Form 10-K, filed with the SEC on March 5, 2024.
(6)
Incorporated by reference to Exhibit 99.1 of the Registrant's Registration Statement on Form S-8 (File No. 333-245764), filed with the SEC on August 13, 2020.
(7)
Incorporated by reference to Exhibit 99.5 of the Registrant's Registration Statement on Form S-8 (File No. 333-227073), filed with the SEC on August 28, 2018.
*
Filed herewith.
SIGNATURES
Pursuant to the requirements of the Securities Act of 1933, as amended, the Registrant certifies that it has reasonable grounds to believe that it meets all of the requirements for filing on Form S-8 and
    has duly caused this Registration Statement to be signed on its behalf by the undersigned, thereunto duly authorized, in the City of San Diego, State of California, on April 1, 2025.
BIONANO GENOMICS, INC.
By:
/s/ R. Erik Holmlin, Ph.D.
R. Erik Holmlin, Ph.D.
President and Chief Executive Officer
POWER OF ATTORNEY
KNOW ALL BY THESE PRESENTS, that each person whose signature appears below constitutes and appoints each of R. Erik Holmlin, Ph.D. and Mark Adamchak as their true and lawful attorney-in-fact and agent,
    with the full power of substitution, for him or her and in his or her name, place or stead, in any and all capacities, to sign any and all amendments to this Registration Statement (including post-effective amendments), and to file the same, with
    exhibits thereto and other documents in connection therewith, with the Securities and Exchange Commission, granting unto said attorney-in-fact and agent full power and authority to do and perform each and every act and thing requisite and necessary to
    be done in and about the premises, as fully to all intents and purposes as he or she might or could do in person, hereby ratifying and confirming all that said attorney-in-fact and agent, or his substitute or substitutes, may lawfully do or cause to be
    done by virtue hereof.
Pursuant to the requirements of the Securities Act of 1933, as amended, this Registration Statement has been signed by the following persons in the capacities and on the dates indicated.
Signature
Title
Date
/s/ R. Erik Holmlin, Ph.D.
President, Chief Executive Officer and Director
(Principal Executive Officer and Principal Financial Officer)
April 1, 2025
R. Erik Holmlin, Ph.D.
/s/ Mark Adamchak
VP, Accounting
(Principal Accounting Officer)
April 1, 2025
Mark Adamchak
/s/ David L. Barker, Ph.D.
Director
April 1, 2025
David L. Barker, Ph.D.
/s/ Yvonne Linney, Ph.D.
Director
April 1, 2025
Yvonne Linney, Ph.D.
/s/ Albert A. Luderer, Ph.D.
Director
April 1, 2025
Albert A. Luderer, Ph.D.
/s/ Hannah Mamuszka
Director
April 1, 2025
Hannah Mamuszka
/s/ Aleksandar Rajkovic, M.D., Ph.D.
Director
April 1, 2025
Aleksandar Rajkovic, M.D., Ph.D.
/s/ Christopher Twomey
Director
April 1, 2025
Christopher Twomey
/s/ Kristiina Vuori, M.D., Ph.D.
Director
April 1, 2025
Kristiina Vuori, M.D., Ph.D.
/s/ Vincent Wong, J.D., M.B.A.
Director
April 1, 2025
Vincent Wong, J.D., M.B.A.