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  <div style="text-align: center; color: rgb(0, 0, 0); font-size: 10pt; font-weight: bold;">As filed with the Securities and Exchange Commission on August 9, 2022</div>
  <div style="text-align: right; color: rgb(0, 0, 0); font-size: 10pt; font-weight: bold;">Registration No. 333-</div>
  <div style="color: rgb(0, 0, 0); font-size: 10pt; font-weight: bold;">
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      <div style="text-align: center; margin-top: 4pt; color: rgb(0, 0, 0); font-size: 14pt; font-weight: bold;">UNITED STATES</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-size: 14pt; font-weight: bold;">SECURITIES AND EXCHANGE COMMISSION</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-size: 12pt; font-weight: bold;">Washington, D.C. 20549</div>
      <div style="text-align: center; margin-top: 6pt; color: rgb(0, 0, 0); font-size: 18pt; font-weight: bold;">FORM S-8</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">REGISTRATION STATEMENT</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-style: italic; font-weight: bold;">Under</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-style: italic; font-weight: bold;">The Securities Act of 1933</div>
      <div style="text-align: center; margin-top: 6pt; color: rgb(0, 0, 0); font-size: 24pt; font-weight: bold;">AVROBIO, INC.</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">(Exact name of registrant as specified in its charter)</div>
      <div><br>
      </div>
      <table cellspacing="0" cellpadding="0" border="0" id="z76d46600fb4f41a79892e5ecb68f44bd" style="font-family: 'Times New Roman'; font-size: 9pt; width: 100%; border-collapse: collapse; text-align: left; color: rgb(0, 0, 0);">

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            <td style="width: 49%; vertical-align: top; font-size: 10pt;">
              <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">Delaware</div>
            </td>
            <td style="width: 2%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
            <td style="width: 49%; vertical-align: top; font-size: 10pt;">
              <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">81-0710585</div>
            </td>
          </tr>
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            <td style="width: 49%; vertical-align: top;">
              <div style="text-align: center; margin-bottom: 1pt; color: rgb(0, 0, 0); font-size: 10pt; font-weight: bold;">(State or other jurisdiction of incorporation or organization)</div>
            </td>
            <td style="width: 2%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
            <td style="width: 49%; vertical-align: top;">
              <div style="text-align: center; margin-bottom: 1pt; color: rgb(0, 0, 0); font-size: 10pt; font-weight: bold;">(I.R.S. Employer Identification Number)</div>
            </td>
          </tr>

      </table>
      <div><br>
      </div>
      <table cellspacing="0" cellpadding="0" border="0" id="z573e676fcbfe499ba9acdad8ffb2df1e" style="font-family: 'Times New Roman'; font-size: 9pt; width: 100%; border-collapse: collapse; text-align: left; color: rgb(0, 0, 0);">

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            <td style="width: 49%; vertical-align: top;">
              <div style="text-align: center; color: rgb(0, 0, 0); font-size: 10pt; font-weight: bold;">100 Technology Square</div>
              <div style="text-align: center; color: rgb(0, 0, 0); font-size: 10pt; font-weight: bold;">Sixth Floor</div>
              <div style="text-align: center; margin-bottom: 1pt; color: rgb(0, 0, 0); font-size: 10pt; font-weight: bold;">Cambridge, Massachusetts</div>
            </td>
            <td style="width: 2%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
            <td style="width: 49%; vertical-align: bottom; font-size: 10pt;">
              <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">02139</div>
            </td>
          </tr>
          <tr>
            <td style="width: 49%; vertical-align: top; font-size: 10pt;">
              <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">(Address of Principal Executive Offices)</div>
            </td>
            <td style="width: 2%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
            <td style="width: 49%; vertical-align: top; font-size: 10pt;">
              <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">(Zip Code)</div>
            </td>
          </tr>

      </table>
      <div style="text-align: center; margin-top: 6pt; color: rgb(0, 0, 0); font-weight: bold;">2018 Stock Option and Incentive Plan</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">(Full Title of the Plans)</div>
      <div style="text-align: center; margin-top: 6pt; color: rgb(0, 0, 0); font-weight: bold;">Geoff MacKay</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">President and Chief Executive Officer</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">100 Technology Square</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">Sixth Floor</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">Cambridge, MA 02139</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">(Name and address of agent for service)</div>
      <div style="text-align: center; margin-top: 6pt; color: rgb(0, 0, 0); font-weight: bold;">(617) 914-8420</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">(Telephone number, including area code, of agent for service)</div>
      <div style="text-align: center; margin-top: 6pt; color: rgb(0, 0, 0); font-style: italic; font-weight: bold;">Copies to:</div>
      <div style="text-align: center; margin-top: 4pt; color: rgb(0, 0, 0); font-weight: bold;">Arthur R. McGivern, Esq.</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">James Xu, Esq.</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">Goodwin Procter LLP</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">100 Northern Avenue</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">Boston, Massachusetts 02210</div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">(617) 570-1000</div>
      <div style="text-align: justify; margin-top: 6pt; font-weight: normal;">Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, a smaller reporting company or an emerging growth
        company. See the definitions of <font style="color: rgb(0, 0, 0);">&#8220;</font>large accelerated filer,<font style="color: rgb(0, 0, 0);">&#8221;</font>&#160;<font style="color: rgb(0, 0, 0);">&#8220;</font>accelerated filer,<font style="color: rgb(0, 0, 0);">&#8221;</font>&#160;<font style="color: rgb(0, 0, 0);">&#8220;</font>smaller reporting company,<font style="color: rgb(0, 0, 0);">&#8221;</font> and <font style="color: rgb(0, 0, 0);">&#8220;</font>emerging growth company<font style="color: rgb(0, 0, 0);">&#8221;</font> in Rule 12b-2 of the
        Exchange Act.</div>
      <div><br>
      </div>
      <table cellspacing="0" cellpadding="0" border="0" align="center" id="z790c4dfebf544830b471044f4e708033" style="border-collapse: collapse; width: 60%; color: rgb(0, 0, 0); font-family: 'Times New Roman'; font-size: 9pt; text-align: left;">

          <tr>
            <td style="width: 22%; vertical-align: bottom; font-size: 10pt;">
              <div>Large accelerated filer</div>
            </td>
            <td style="width: 8.5%; vertical-align: bottom; font-size: 10pt;">
              <div style="text-align: center; color: rgb(0, 0, 0);">&#9744;</div>
            </td>
            <td style="width: 22%; vertical-align: bottom; font-size: 10pt;">
              <div>Accelerated filer</div>
            </td>
            <td style="width: 8%; vertical-align: bottom; font-size: 10pt;">
              <div style="text-align: center; color: rgb(0, 0, 0);">&#9744;</div>
            </td>
          </tr>
          <tr>
            <td style="width: 22%; vertical-align: middle; font-size: 10pt;">&#160;</td>
            <td style="width: 8.5%; vertical-align: middle; font-size: 10pt; text-align: center;">&#160;</td>
            <td style="width: 22%; vertical-align: middle; font-size: 10pt;">&#160;</td>
            <td style="width: 8%; vertical-align: middle; font-size: 10pt; text-align: center;">&#160;</td>
          </tr>
          <tr>
            <td style="width: 22%; vertical-align: bottom; font-size: 10pt;">
              <div>Non-accelerated filer</div>
            </td>
            <td style="width: 8.5%; vertical-align: bottom; font-size: 10pt;">
              <div style="text-align: center; color: rgb(0, 0, 0);">&#9746;</div>
            </td>
            <td style="width: 22%; vertical-align: bottom; font-size: 10pt;">
              <div>Smaller reporting company</div>
            </td>
            <td style="width: 8%; vertical-align: bottom; font-size: 10pt;">
              <div style="text-align: center; color: rgb(0, 0, 0);">&#9746;</div>
            </td>
          </tr>
          <tr>
            <td style="width: 22%; vertical-align: middle; font-size: 10pt;">&#160;</td>
            <td style="width: 8.5%; vertical-align: middle; font-size: 10pt; text-align: center;">&#160;</td>
            <td style="width: 22%; vertical-align: middle; font-size: 10pt;">&#160;</td>
            <td style="width: 8%; vertical-align: middle; font-size: 10pt; text-align: center;">&#160;</td>
          </tr>
          <tr>
            <td style="width: 22%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
            <td style="width: 8.5%; vertical-align: bottom; font-size: 10pt; text-align: center;">&#160;</td>
            <td style="width: 22%; vertical-align: bottom; font-size: 10pt;">
              <div style="text-indent: -10pt; margin-left: 10pt;">Emerging growth company</div>
            </td>
            <td style="width: 8%; vertical-align: bottom; font-size: 10pt;">
              <div style="text-align: center; text-indent: -10pt; margin-left: 10pt; color: rgb(0, 0, 0);">&#9746;</div>
            </td>
          </tr>

      </table>
      <div style="font-weight: normal; text-align: justify;"> <br>
      </div>
      <div style="font-weight: normal; text-align: justify;">If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards
        provided pursuant to Section 7(a)(2)(B) of the Securities Act. <font style="color: rgb(0, 0, 0);">&#9744;</font></div>
      <div style="font-weight: normal; text-align: justify;"><font style="color: rgb(0, 0, 0);"> <br>
        </font></div>
      <div style="font-weight: normal; text-align: justify;">
        <hr align="center" style="border: none; border-bottom: 4px solid black; border-top: 1px solid black; height: 10px; color: #ffffff; background-color: #ffffff; text-align: center; margin-left: auto; margin-right: auto;"><font style="color: rgb(0, 0, 0);"> </font></div>
      <div id="DSPFPageBreakArea" style="clear: both; margin-top: 9pt; margin-bottom: 9pt;">
        <div id="DSPFPageBreak" style="page-break-after:always;">
          <hr noshade="noshade" style="margin: 4px 0px; width: 100%; border-width: 0; height: 2px; color: #000000; background-color: #000000; clear: both;"></div>
      </div>
      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">EXPLANATORY NOTE</div>
      <div>
        <div style="text-align: justify;"><font style="color: rgb(0, 0, 0); font-weight: normal;"><br>
            This Registration Statement on Form S-8 registers additional shares of common stock under the Registrant&#8217;s 2018 Stock Option and Incentive Plan, as amended (the &#8220;2018 Plan&#8221;). </font><font style="font-weight: normal;">The 3,700,000 additional
            shares represent an increase in the number of shares of common stock reserved for issuance under the 2018 Plan, which increase was approved by the Registrant&#8217;s stockholders on June 8, 2022 at the Registrant&#8217;s 2022 Annual Meeting of
            Stockholders.</font></div>
        <div style="text-align: justify;"><font style="font-weight: normal;"> <br>
          </font></div>
        <div style="text-align: justify;"><font style="color: rgb(0, 0, 0); font-weight: normal;">The additional shares described above are of the same class as other securities relating to the 2018 Plan for which the Registrant&#8217;s registration statements
            filed on Form S-8 (Registration No. 333-225788) on June 21, 2018, on Form S-8 (Registration No. 333-230494) on March 25, 2019, on Form S-8 </font><font style="font-weight: normal;">(Registration No. 333-237203) on March 16, 2020, on Form S-8
            (Registration No. 333-241400) on August 6, 2020, on Form S-8 (Registration No. 333-254466) on March 18, 2021 and on Form S-8 (Registration No. 333-263655) on March 17, 2022<font style="color: rgb(0, 0, 0);">, are effective. The information
              contained in the Registrant&#8217;s registration statement on Form S-8 (Registration No. 333-225788) is hereby incorporated by reference pursuant to General Instruction E.</font></font></div>
      </div>
      <div><br>
      </div>
      <div id="DSPFPageBreakArea" style="clear: both; margin-top: 9pt; margin-bottom: 9pt;">
        <div id="DSPFPageBreak" style="page-break-after:always;">
          <hr noshade="noshade" style="margin: 4px 0px; width: 100%; border-width: 0; height: 2px; color: #000000; background-color: #000000; clear: both;"></div>
      </div>
      <div>
        <div style="color: rgb(0, 0, 0); font-weight: bold; text-align: center;">Part II</div>
      </div>
      <div>
        <div style="color: #000000; font-weight: bold; text-align: center;"> <br>
        </div>
        <div style="color: #000000; font-weight: bold; text-align: center;">INFORMATION REQUIRED IN THE REGISTRATION STATEMENT</div>
      </div>
      <div><br>
      </div>
      <table cellspacing="0" cellpadding="0" id="zc4d0d923a19e4ba5acd99e9e4193ec77" class="DSPFListTable" style="font-family: 'Times New Roman'; font-size: 9pt; width: 100%; text-align: left; color: rgb(0, 0, 0);">

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            <td style="width: 45pt; vertical-align: top; color: rgb(0, 0, 0); font-size: 10pt; font-weight: bold;">Item 8.</td>
            <td style="width: auto; vertical-align: top; font-size: 10pt;">
              <div style="color: rgb(0, 0, 0); font-weight: bold;">Exhibits.</div>
            </td>
          </tr>

      </table>
      <div style="text-align: center; margin-top: 6pt; color: rgb(0, 0, 0); font-weight: bold;">EXHIBIT INDEX</div>
      <div style="text-align: center; margin-top: 6pt; color: rgb(0, 0, 0); font-weight: bold;"> <br>
      </div>
      <table cellspacing="0" cellpadding="0" border="0" id="z5a29201749784c4a965275bfb9e44f6d" style="font-family: 'Times New Roman'; font-size: 9pt; width: 100%; border-collapse: collapse; text-align: left; color: rgb(0, 0, 0);">

          <tr>
            <td style="width: 7.39%; vertical-align: bottom; border-bottom: 2px solid rgb(0, 0, 0);">
              <div style="text-align: center; color: rgb(0, 0, 0); font-size: 10pt; font-weight: bold;">Exhibit</div>
              <div style="text-align: center; color: rgb(0, 0, 0); font-size: 10pt; font-weight: bold;">No.</div>
            </td>
            <td style="width: 3%; vertical-align: bottom; padding-bottom: 2px; font-size: 10pt;">&#160;</td>
            <td style="width: 90%; vertical-align: bottom; text-align: center; border-bottom: 2px solid rgb(0, 0, 0);">
              <div><font style="font-weight: bold; font-size: 10pt;">Description </font><font style="font-size: 10pt;"><br>
                </font></div>
            </td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: middle; font-size: 10pt;">&#160;</td>
            <td style="vertical-align: middle; font-size: 10pt;" colspan="2">&#160;</td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: top; font-size: 10pt;">
              <div>4.1</div>
            </td>
            <td style="width: 3%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
            <td style="width: 90%; vertical-align: top; font-size: 10pt;">
              <div style="text-align: justify;"><a href="https://www.sec.gov/Archives/edgar/data/1681087/000119312518202460/d153924dex31.htm">Fourth Amended and Restated Certificate of Incorporation of the Registrant (filed as Exhibit 3.1 to the
                  Registrant&#8217;s Current Report on Form 8-K filed on June 25, 2018 (File No. 001-38537) and incorporated herein by reference)</a></div>
            </td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: middle; font-size: 10pt;">&#160;</td>
            <td style="vertical-align: middle; font-size: 10pt;" colspan="2">&#160;</td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: top; font-size: 10pt;">
              <div>4.2</div>
            </td>
            <td style="width: 3%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
            <td style="width: 90%; vertical-align: top; font-size: 10pt;">
              <div style="text-align: justify;"><a href="https://www.sec.gov/Archives/edgar/data/1681087/000119312518202460/d153924dex32.htm">Amended and Restated By-laws (filed as Exhibit 3.2 to the Registrant&#8217;s Current Report on Form 8-K filed on June
                  25, 2018 (File No. 001-38537) and incorporated herein by reference)</a></div>
            </td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: middle; font-size: 10pt;">&#160;</td>
            <td style="vertical-align: middle; font-size: 10pt;" colspan="2">&#160;</td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: top; font-size: 10pt;">
              <div>4.3</div>
            </td>
            <td style="width: 3%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
            <td style="width: 90%; vertical-align: top; font-size: 10pt;">
              <div style="text-align: justify;"><a href="https://www.sec.gov/Archives/edgar/data/1681087/000119312518175024/d562006dex42.htm">Second Amended and Restated Investors&#8217; Rights Agreement among the Registrant and certain of its stockholders,
                  dated January 9, 2018 (filed as Exhibit 4.2 to the Registrant&#8217;s Registration Statement on Form S-1 filed on May 25, 2018 (File No. 333-225213) and incorporated herein by reference)</a></div>
            </td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: middle; font-size: 10pt;">&#160;</td>
            <td style="vertical-align: middle; font-size: 10pt;" colspan="2">&#160;</td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: top; font-size: 10pt;">
              <div>5.1*</div>
            </td>
            <td style="width: 3%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
            <td style="width: 90%; vertical-align: top; font-size: 10pt;">
              <div style="text-align: justify;"><a href="brhc10040000_ex5-1.htm">Opinion of Goodwin Procter LLP</a></div>
            </td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: middle; font-size: 10pt;">&#160;</td>
            <td style="vertical-align: middle; font-size: 10pt;" colspan="2">&#160;</td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: top; font-size: 10pt;">
              <div>23.1*</div>
            </td>
            <td style="width: 3%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
            <td style="width: 90%; vertical-align: top; font-size: 10pt;">
              <div style="text-align: justify;"><a href="brhc10040000_ex23-1.htm">Consent of Ernst &amp; Young LLP, Independent Registered Public Accounting Firm</a></div>
            </td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: middle; font-size: 10pt;">&#160;</td>
            <td style="vertical-align: middle; font-size: 10pt;" colspan="2">&#160;</td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: top; font-size: 10pt;">
              <div>23.2*</div>
            </td>
            <td style="width: 3%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
            <td style="width: 90%; vertical-align: top; font-size: 10pt;">
              <div style="text-align: justify;"><a href="brhc10040000_ex5-1.htm">Consent of Goodwin Procter LLP (included in Exhibit 5.1)</a></div>
            </td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: middle; font-size: 10pt;">&#160;</td>
            <td style="vertical-align: middle; font-size: 10pt;" colspan="2">&#160;</td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: top; font-size: 10pt;">
              <div>24.1*</div>
            </td>
            <td style="width: 3%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
            <td style="width: 90%; vertical-align: top; font-size: 10pt;">
              <div style="text-align: justify;"><a href="#POWEROFATTORNEYANDSIGNATU">Power of Attorney (included on signature page)</a></div>
            </td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: middle; font-size: 10pt;">&#160;</td>
            <td style="vertical-align: middle; font-size: 10pt;" colspan="2">&#160;</td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: top; font-size: 10pt;">
              <div>99.1</div>
            </td>
            <td style="width: 3%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
            <td style="width: 90%; vertical-align: top; font-size: 10pt;">
              <div style="text-align: justify;"><a href="https://www.sec.gov/Archives/edgar/data/1681087/000119312518188630/d562006dex102.htm">2018 Stock Option and Incentive Plan and forms of award agreements thereunder (filed as Exhibit 10.2 to the
                  Registrant&#8217;s Second Amendment to the Registration Statement on Form S-1 filed on June 11, 2018 (File No. 333-225213) and incorporated herein by reference)</a></div>
            </td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: top; font-size: 10pt;">&#160;</td>
            <td style="width: 3%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
            <td style="width: 90%; vertical-align: top; font-size: 10pt;">&#160;</td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: top; font-size: 10pt;">
              <div>99.2</div>
            </td>
            <td style="width: 3%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
            <td style="width: 90%; vertical-align: top; font-size: 10pt;">
              <div style="text-align: justify;"><a href="https://www.sec.gov/Archives/edgar/data/0001681087/000156459020028774/avro-ex101_30.htm">First Amendment to the Stock Option and Incentive Plan (filed as Exhibit 10.1 to the Registrant&#8217;s Current
                  Report on Form 8-K filed on June 9, 2020 (File No. 001-38537) and incorporated herein by reference)</a></div>
            </td>
          </tr>
          <tr>
            <td style="width: 7.39%; vertical-align: top; font-size: 10pt;">&#160;</td>
            <td style="width: 3%; vertical-align: bottom; font-size: 10pt;">&#160;</td>
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            <td style="width: 90%; vertical-align: top; font-size: 10pt;">
              <div style="text-align: justify;"><a href="https://www.sec.gov/Archives/edgar/data/1681087/000156459022022999/avro-ex101_6.htm">Second Amendment to the Stock Option and Incentive Plan (filed as Exhibit 10.1 to the Registrant&#8217;s Current Report
                  on Form 8-K filed on June 10, 2022 (File No. 001-38537) and incorporated herein by reference)</a></div>
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            <td style="width: 7.39%; vertical-align: top; font-size: 10pt;">&#160;</td>
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            <td style="width: 90%; vertical-align: top; font-size: 10pt;">
              <div style="text-align: justify;"><a href="brhc10040000_ex107.htm">Filing Fee Table</a></div>
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              <div>Filed herewith.</div>
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      <div style="text-align: center; color: rgb(0, 0, 0); font-weight: bold;">SIGNATURES<br>
        <br>
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      <div style="font-weight: normal; text-align: justify;">Pursuant to the requirements of the Securities Act, the registrant certifies that it has reasonable grounds to believe that it meets all of the requirements for filing on Form S-8 and has duly
        caused this Registration Statement to be signed on its behalf by the undersigned, thereunto duly authorized, in the City of Cambridge, Commonwealth of Massachusetts, on the 9<sup style="vertical-align: text-top; line-height: 1; font-size: smaller;">th</sup> day of August, 2022.</div>
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            <td style="width: 3%; vertical-align: middle; font-size: 10pt;" rowspan="1" colspan="3">
              <div style="color: rgb(0, 0, 0); font-weight: bold;">AVROBIO, INC.</div>
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            <td style="vertical-align: middle; font-size: 10pt; width: 1%;" colspan="1">&#160;</td>
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              <div>By:</div>
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            <td style="width: 1%; vertical-align: top; font-size: 10pt; border-bottom: 2px solid rgb(0, 0, 0);" colspan="1">&#160;</td>
            <td style="width: 46%; vertical-align: top; font-size: 10pt; border-bottom: 2px solid rgb(0, 0, 0);">
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      <div style="color: #000000; font-weight: bold; text-align: center;"> <br>
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      <div style="color: #000000; font-weight: bold; text-align: center;"><a name="POWEROFATTORNEYANDSIGNATU"><!--Anchor--></a>POWER OF ATTORNEY AND SIGNATURES</div>
      <div style="text-align: justify; margin-top: 12pt; font-weight: normal;">KNOW ALL BY THESE PRESENT, that each individual whose signature appears below hereby constitutes and appoints each of Geoff MacKay and Erik Ostrowski as such person<font style="color: rgb(0, 0, 0);">&#8217;</font>s true and lawful attorney-in-fact and agent with full power of substitution and resubstitution, for such person in such person<font style="color: rgb(0, 0, 0);">&#8217;</font>s name, place and stead, in any and all
        capacities, to sign any and all amendments (including post-effective amendments) to this Registration Statement on Form S-8, and to file the same, with all exhibits thereto, and all documents in connection therewith, with the Commission granting
        unto each said attorney-in-fact and agent full power and authority to do and perform each and every act and thing requisite and necessary to be done in and about the premises, as fully to all intents and purposes as such person might or could do in
        person, hereby ratifying and confirming all that any said attorney-in-fact and agent, or any substitute or substitutes of any of them, may lawfully do or cause to be done by virtue hereof.</div>
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      <div style="font-weight: normal; text-align: justify;">Pursuant to the requirements of the Securities Act, this Registration Statement has been signed by the following persons in the capacities and on the date indicated.</div>
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              <div style="text-align: center; color: rgb(0, 0, 0); font-size: 10pt; font-style: italic;">Director, President, Chief Executive Officer and</div>
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              <div style="text-align: center;">August 9, 2022</div>
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              <div style="text-align: center;">August 9, 2022</div>
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              <div style="text-align: center;">August 9, 2022</div>
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              <div style="text-align: center;">August 9, 2022</div>
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              <div style="text-align: center;">August 9, 2022</div>
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              <div style="text-align: center;">August 9, 2022</div>
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              <div style="text-align: center;">August 9, 2022</div>
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              <div style="text-align: center;">August 9, 2022</div>
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As filed with the Securities and Exchange Commission on August 9, 2022
Registration No. 333-
UNITED STATES
SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549
FORM S-8
REGISTRATION STATEMENT
Under
The Securities Act of 1933
AVROBIO, INC.
(Exact name of registrant as specified in its charter)
Delaware
81-0710585
(State or other jurisdiction of incorporation or organization)
(I.R.S. Employer Identification Number)
100 Technology Square
Sixth Floor
Cambridge, Massachusetts
02139
(Address of Principal Executive Offices)
(Zip Code)
2018 Stock Option and Incentive Plan
(Full Title of the Plans)
Geoff MacKay
President and Chief Executive Officer
100 Technology Square
Sixth Floor
Cambridge, MA 02139
(Name and address of agent for service)
(617) 914-8420
(Telephone number, including area code, of agent for service)
Copies to:
Arthur R. McGivern, Esq.
James Xu, Esq.
Goodwin Procter LLP
100 Northern Avenue
Boston, Massachusetts 02210
(617) 570-1000
Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, a smaller reporting company or an emerging growth
        company. See the definitions of
"
large accelerated filer,
"
"
accelerated filer,
"
"
smaller reporting company,
"
and
"
emerging growth company
"
in Rule 12b-2 of the
        Exchange Act.
Large accelerated filer
☐
Accelerated filer
☐
Non-accelerated filer
☒
Smaller reporting company
☒
Emerging growth company
☒
If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards
        provided pursuant to Section 7(a)(2)(B) of the Securities Act.
☐
EXPLANATORY NOTE
This Registration Statement on Form S-8 registers additional shares of common stock under the Registrant's 2018 Stock Option and Incentive Plan, as amended (the "2018 Plan").
The 3,700,000 additional
            shares represent an increase in the number of shares of common stock reserved for issuance under the 2018 Plan, which increase was approved by the Registrant's stockholders on June 8, 2022 at the Registrant's 2022 Annual Meeting of
            Stockholders.
The additional shares described above are of the same class as other securities relating to the 2018 Plan for which the Registrant's registration statements
            filed on Form S-8 (Registration No. 333-225788) on June 21, 2018, on Form S-8 (Registration No. 333-230494) on March 25, 2019, on Form S-8
(Registration No. 333-237203) on March 16, 2020, on Form S-8
            (Registration No. 333-241400) on August 6, 2020, on Form S-8 (Registration No. 333-254466) on March 18, 2021 and on Form S-8 (Registration No. 333-263655) on March 17, 2022
, are effective. The information
              contained in the Registrant's registration statement on Form S-8 (Registration No. 333-225788) is hereby incorporated by reference pursuant to General Instruction E.
Part II
INFORMATION REQUIRED IN THE REGISTRATION STATEMENT
Item 8.
Exhibits.
EXHIBIT INDEX
Exhibit
No.
Description
4.1
Fourth Amended and Restated Certificate of Incorporation of the Registrant (filed as Exhibit 3.1 to the
                  Registrant's Current Report on Form 8-K filed on June 25, 2018 (File No. 001-38537) and incorporated herein by reference)
4.2
Amended and Restated By-laws (filed as Exhibit 3.2 to the Registrant's Current Report on Form 8-K filed on June
                  25, 2018 (File No. 001-38537) and incorporated herein by reference)
4.3
Second Amended and Restated Investors' Rights Agreement among the Registrant and certain of its stockholders,
                  dated January 9, 2018 (filed as Exhibit 4.2 to the Registrant's Registration Statement on Form S-1 filed on May 25, 2018 (File No. 333-225213) and incorporated herein by reference)
5.1*
Opinion of Goodwin Procter LLP
23.1*
Consent of Ernst & Young LLP, Independent Registered Public Accounting Firm
23.2*
Consent of Goodwin Procter LLP (included in Exhibit 5.1)
24.1*
Power of Attorney (included on signature page)
99.1
2018 Stock Option and Incentive Plan and forms of award agreements thereunder (filed as Exhibit 10.2 to the
                  Registrant's Second Amendment to the Registration Statement on Form S-1 filed on June 11, 2018 (File No. 333-225213) and incorporated herein by reference)
99.2
First Amendment to the Stock Option and Incentive Plan (filed as Exhibit 10.1 to the Registrant's Current
                  Report on Form 8-K filed on June 9, 2020 (File No. 001-38537) and incorporated herein by reference)
99.3
Second Amendment to the Stock Option and Incentive Plan (filed as Exhibit 10.1 to the Registrant's Current Report
                  on Form 8-K filed on June 10, 2022 (File No. 001-38537) and incorporated herein by reference)
107*
Filing Fee Table
*
Filed herewith.
SIGNATURES
Pursuant to the requirements of the Securities Act, the registrant certifies that it has reasonable grounds to believe that it meets all of the requirements for filing on Form S-8 and has duly
        caused this Registration Statement to be signed on its behalf by the undersigned, thereunto duly authorized, in the City of Cambridge, Commonwealth of Massachusetts, on the 9
th
day of August, 2022.
AVROBIO, INC.
By:
/s/ Geoff MacKay
Geoff MacKay
President, Chief Executive Officer and
Principal Executive Officer
POWER OF ATTORNEY AND SIGNATURES
KNOW ALL BY THESE PRESENT, that each individual whose signature appears below hereby constitutes and appoints each of Geoff MacKay and Erik Ostrowski as such person
'
s true and lawful attorney-in-fact and agent with full power of substitution and resubstitution, for such person in such person
'
s name, place and stead, in any and all
        capacities, to sign any and all amendments (including post-effective amendments) to this Registration Statement on Form S-8, and to file the same, with all exhibits thereto, and all documents in connection therewith, with the Commission granting
        unto each said attorney-in-fact and agent full power and authority to do and perform each and every act and thing requisite and necessary to be done in and about the premises, as fully to all intents and purposes as such person might or could do in
        person, hereby ratifying and confirming all that any said attorney-in-fact and agent, or any substitute or substitutes of any of them, may lawfully do or cause to be done by virtue hereof.
Pursuant to the requirements of the Securities Act, this Registration Statement has been signed by the following persons in the capacities and on the date indicated.
Name
Title
Date
/s/ Geoff MacKay
Director, President, Chief Executive Officer and
Principal Executive Officer
August 9, 2022
Geoff MacKay
/s/ Erik Ostrowski
Chief Financial Officer and Principal Financial
and Accounting Officer
August 9, 2022
Erik Ostrowski
/s/ Bruce Booth
Chairman of the Board of Directors
August 9, 2022
Bruce Booth, D.Phil.
/s/ Ian T. Clark
Director
August 9, 2022
Ian T. Clark
/s/ Phillip B. Donenberg
Director
August 9, 2022
Phillip B. Donenberg
/s/ Gail M. Farfel
Director
August 9, 2022
Gail M. Farfel, Ph.D.
/s/ Annalisa Jenkins
Director
August 9, 2022
Annalisa Jenkins, M.B.B.S., F.R.C.P.
/s/ Christopher Paige
Director
August 9, 2022
Christopher Paige, Ph.D.
/s/ Philip Vickers
Director
August 9, 2022
Philip Vickers, Ph.D.