content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001851981</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001823593</issuerCik>
        <issuerName>TuSimple Holdings Inc.</issuerName>
        <secFileNumber>001-40326</secFileNumber>
        <issuerAddress>
          <com:street1>9191 TOWNE CENTRE DRIVE, SUITE 150</com:street1>
          <com:city>SAN DIEGO</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>92122</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>(619) 916-3144</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Lu Cheng</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Officer</relationshipToIssuer>
          <relationshipToIssuer>Director</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Class A</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>Fidelity Brokerage Services LLC</name>
          <address>
            <com:street1>900 Salem Street</com:street1>
            <com:city>Smithfield</com:city>
            <com:stateOrCountry>RI</com:stateOrCountry>
            <com:zipCode>02917</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>129665</noOfUnitsSold>
        <aggregateMarketValue>52060.50</aggregateMarketValue>
        <noOfUnitsOutstanding>205890683</noOfUnitsOutstanding>
        <approxSaleDate>01/23/2024</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Class A</securitiesClassTitle>
        <acquiredDate>01/22/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>129665</amountOfSecuritiesAcquired>
        <paymentDate>01/22/2024</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>01/23/2024</noticeDate>
        <signature>s/ Emily Navaro, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Cheng Lu.</signature>
      </noticeSignature>
    </formData>
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text: 144
  0001851981
  XXXXXXXX
  LIVE
  0001823593
  TuSimple Holdings Inc.
  001-40326
  9191 TOWNE CENTRE DRIVE, SUITE 150
  SAN DIEGO
  CA
  92122
  (619) 916-3144
  Lu Cheng
  Officer
  Director
  Class A
  Fidelity Brokerage Services LLC
  900 Salem Street
  Smithfield
  RI
  02917
  129665
  52060.50
  205890683
  01/23/2024
  NASDAQ
  Class A
  01/22/2024
  Restricted Stock Vesting
  Issuer
  N
  129665
  01/22/2024
  Compensation
  Y
  01/23/2024
  s/ Emily Navaro, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Cheng Lu.