content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
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      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001463488</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
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    <formData>
      <issuerInfo>
        <issuerCik>0001582961</issuerCik>
        <issuerName>DigitalOcean Holdings, Inc.</issuerName>
        <secFileNumber>001-40252</secFileNumber>
        <issuerAddress>
          <com:street1>101 Avenue of the Americas, 10th Floor</com:street1>
          <com:city>New York</com:city>
          <com:stateOrCountry>NY</com:stateOrCountry>
          <com:zipCode>10013</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>341-985-0306</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Yancey L. Spruill</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Former Affiliate</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common Stock</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>J.P. Morgan Securities LLC</name>
          <address>
            <com:street1>390 Madison Avenue</com:street1>
            <com:street2>6th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10017</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>300000</noOfUnitsSold>
        <aggregateMarketValue>11727000</aggregateMarketValue>
        <noOfUnitsOutstanding>90796695</noOfUnitsOutstanding>
        <approxSaleDate>03/20/2024</approxSaleDate>
        <securitiesExchangeName>NYSE</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common Stock</securitiesClassTitle>
        <acquiredDate>02/23/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Transfer</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Yancey Spruill, who acquired the shares from compensation received from the Issuer on 11/15/23</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>300000</amountOfSecuritiesAcquired>
        <paymentDate>02/23/2024</paymentDate>
        <natureOfPayment>N/A</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Yancey L. Spruill Revocable Trust Dated 09/02/20</name>
          <address>
            <com:street1>C/o DigitalOcean Holdings, Inc.</com:street1>
            <com:street2>101 Avenue of the Americas, 10th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10013</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common Stock</securitiesClassTitle>
        <saleDate>02/26/2024</saleDate>
        <amountOfSecuritiesSold>225000</amountOfSecuritiesSold>
        <grossProceeds>8189180</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Yancey L. Spruill Revocable Trust Dated 09/02/20</name>
          <address>
            <com:street1>C/o DigitalOcean Holdings, Inc.</com:street1>
            <com:street2>101 Avenue of the Americas, 10th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10013</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common Stock</securitiesClassTitle>
        <saleDate>02/27/2024</saleDate>
        <amountOfSecuritiesSold>200000</amountOfSecuritiesSold>
        <grossProceeds>169945</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Yancey L. Spruill Revocable Trust Dated 09/02/20</name>
          <address>
            <com:street1>C/o DigitalOcean Holdings, Inc.</com:street1>
            <com:street2>101 Avenue of the Americas, 10th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10013</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common Stock</securitiesClassTitle>
        <saleDate>02/29/2024</saleDate>
        <amountOfSecuritiesSold>250000</amountOfSecuritiesSold>
        <grossProceeds>247431</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Yancey L. Spruill Revocable Trust Dated 09/02/20</name>
          <address>
            <com:street1>C/o DigitalOcean Holdings, Inc.</com:street1>
            <com:street2>101 Avenue of the Americas, 10th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10013</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common Stock</securitiesClassTitle>
        <saleDate>03/12/2024</saleDate>
        <amountOfSecuritiesSold>600000</amountOfSecuritiesSold>
        <grossProceeds>810963</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Yancey L. Spruill Revocable Trust Dated 09/02/20</name>
          <address>
            <com:street1>C/o DigitalOcean Holdings, Inc.</com:street1>
            <com:street2>101 Avenue of the Americas, 10th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10013</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common Stock</securitiesClassTitle>
        <saleDate>03/19/2024</saleDate>
        <amountOfSecuritiesSold>300000</amountOfSecuritiesSold>
        <grossProceeds>987698</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Yancey L. Spruill Revocable Trust Dated 09/02/20</name>
          <address>
            <com:street1>C/o DigitalOcean Holdings, Inc.</com:street1>
            <com:street2>101 Avenue of the Americas, 10th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10013</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common Stock</securitiesClassTitle>
        <saleDate>03/19/2024</saleDate>
        <amountOfSecuritiesSold>3771</amountOfSecuritiesSold>
        <grossProceeds>151036</grossProceeds>
      </securitiesSoldInPast3Months>
      <remarks>This filing reflects the covering of 3,000 contracts by the use of 300,000 shares of DigitalOcean control shares.  Some of the shares sold in last 3 months represent a total of 13,500 listed option contracts sold.  The shares filed today will be sold by the Yancey L. Spruill Revocable Trust Dated 09/02/20, Yancey L. Spruill as Trustee.</remarks>
      <noticeSignature>
        <noticeDate>03/20/2024</noticeDate>
        <signature>/s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Yancey L. Spruill</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
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text: 144
  0001463488
  XXXXXXXX
  LIVE
  0001582961
  DigitalOcean Holdings, Inc.
  001-40252
  101 Avenue of the Americas, 10th Floor
  New York
  NY
  10013
  341-985-0306
  Yancey L. Spruill
  Former Affiliate
  Common Stock
  J.P. Morgan Securities LLC
  390 Madison Avenue
  6th Floor
  New York
  NY
  10017
  300000
  11727000
  90796695
  03/20/2024
  NYSE
  Common Stock
  02/23/2024
  Transfer
  Yancey Spruill, who acquired the shares from compensation received from the Issuer on 11/15/23
  N
  300000
  02/23/2024
  N/A
  N
  Yancey L. Spruill Revocable Trust Dated 09/02/20
  C/o DigitalOcean Holdings, Inc.
  101 Avenue of the Americas, 10th Floor
  New York
  NY
  10013
  Common Stock
  02/26/2024
  225000
  8189180
  Yancey L. Spruill Revocable Trust Dated 09/02/20
  C/o DigitalOcean Holdings, Inc.
  101 Avenue of the Americas, 10th Floor
  New York
  NY
  10013
  Common Stock
  02/27/2024
  200000
  169945
  Yancey L. Spruill Revocable Trust Dated 09/02/20
  C/o DigitalOcean Holdings, Inc.
  101 Avenue of the Americas, 10th Floor
  New York
  NY
  10013
  Common Stock
  02/29/2024
  250000
  247431
  Yancey L. Spruill Revocable Trust Dated 09/02/20
  C/o DigitalOcean Holdings, Inc.
  101 Avenue of the Americas, 10th Floor
  New York
  NY
  10013
  Common Stock
  03/12/2024
  600000
  810963
  Yancey L. Spruill Revocable Trust Dated 09/02/20
  C/o DigitalOcean Holdings, Inc.
  101 Avenue of the Americas, 10th Floor
  New York
  NY
  10013
  Common Stock
  03/19/2024
  300000
  987698
  Yancey L. Spruill Revocable Trust Dated 09/02/20
  C/o DigitalOcean Holdings, Inc.
  101 Avenue of the Americas, 10th Floor
  New York
  NY
  10013
  Common Stock
  03/19/2024
  3771
  151036
  This filing reflects the covering of 3,000 contracts by the use of 300,000 shares of DigitalOcean control shares.  Some of the shares sold in last 3 months represent a total of 13,500 listed option contracts sold.  The shares filed today will be sold by the Yancey L. Spruill Revocable Trust Dated 09/02/20, Yancey L. Spruill as Trustee.
  03/20/2024
  /s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Yancey L. Spruill