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            <ccc>XXXXXXXX</ccc>
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        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
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        <issuerAddress>
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          <com:city>HELLERUP</com:city>
          <com:stateOrCountry>G7</com:stateOrCountry>
          <com:zipCode>DK-2900</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>45 70 22 22 44</issuerContactPhone>
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        <relationshipsToIssuer>
          <relationshipToIssuer>Officer</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>American Depositary Shares</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>Merrill Lynch</name>
          <address>
            <com:street1>225 Liberty Street</com:street1>
            <com:street2>Floor 37</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10281</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>5625</noOfUnitsSold>
        <aggregateMarketValue>866545.27</aggregateMarketValue>
        <noOfUnitsOutstanding>57707439</noOfUnitsOutstanding>
        <approxSaleDate>02/27/2024</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
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        <securitiesClassTitle>American Depositary Shares</securitiesClassTitle>
        <acquiredDate>02/27/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Warrant exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Ascendis Pharma A/S</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>5625</amountOfSecuritiesAcquired>
        <paymentDate>02/27/2024</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>02/27/2024</noticeDate>
        <signature>LOTTE SONDERBJERG</signature>
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text: 144
  0001965755
  XXXXXXXX
  LIVE
  0001612042
  Ascendis Pharma A/S
  001-36815
  TUBORG BOULEVARD 12
  HELLERUP
  G7
  DK-2900
  45 70 22 22 44
  PEDERSEN ANNI LOTTE KIRSTINE SONDERBJERG
  Officer
  American Depositary Shares
  Merrill Lynch
  225 Liberty Street
  Floor 37
  New York
  NY
  10281
  5625
  866545.27
  57707439
  02/27/2024
  NASDAQ
  American Depositary Shares
  02/27/2024
  Warrant exercise
  Ascendis Pharma A/S
  N
  5625
  02/27/2024
  Cash
  Y
  02/27/2024
  LOTTE SONDERBJERG