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        <filer>
          <filerCredentials>
            <cik>0001965622</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
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        <issuerCik>0001612042</issuerCik>
        <issuerName>Ascendis Pharma A/S</issuerName>
        <secFileNumber>001-36815</secFileNumber>
        <issuerAddress>
          <com:street1>TUBORG BOULEVARD 12</com:street1>
          <com:city>HELLERUP</com:city>
          <com:stateOrCountry>G7</com:stateOrCountry>
          <com:zipCode>DK-2900</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>45 70 22 22 44</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>JENSEN MICHAEL WOLFF</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Officer</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>American Depository Shares (ADSs)</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>Citigroup Global Markets Inc.</name>
          <address>
            <com:street1>390 Greenwich Street</com:street1>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10013</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>927</noOfUnitsSold>
        <aggregateMarketValue>87147.27</aggregateMarketValue>
        <noOfUnitsOutstanding>57328548</noOfUnitsOutstanding>
        <approxSaleDate>05/05/2023</approxSaleDate>
        <securitiesExchangeName>NasdaqGS</securitiesExchangeName>
      </securitiesInformation>
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        <securitiesClassTitle>ADSs</securitiesClassTitle>
        <acquiredDate>12/09/2022</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Unit Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Ascendis Pharma A/S</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>927</amountOfSecuritiesAcquired>
        <paymentDate>12/09/2022</paymentDate>
        <natureOfPayment>Services Rendered</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>05/05/2023</noticeDate>
        <signature>/s/ Michael Wolff Jensen</signature>
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text: 144
  0001965622
  XXXXXXXX
  LIVE
  0001612042
  Ascendis Pharma A/S
  001-36815
  TUBORG BOULEVARD 12
  HELLERUP
  G7
  DK-2900
  45 70 22 22 44
  JENSEN MICHAEL WOLFF
  Officer
  American Depository Shares (ADSs)
  Citigroup Global Markets Inc.
  390 Greenwich Street
  New York
  NY
  10013
  927
  87147.27
  57328548
  05/05/2023
  NasdaqGS
  ADSs
  12/09/2022
  Restricted Stock Unit Vesting
  Ascendis Pharma A/S
  N
  927
  12/09/2022
  Services Rendered
  Y
  05/05/2023
  /s/ Michael Wolff Jensen