content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <!-- Generated by Workiva -->
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001463488</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001582961</issuerCik>
        <issuerName>DigitalOcean Holdings, Inc.</issuerName>
        <secFileNumber>001-40252</secFileNumber>
        <issuerAddress>
          <com:street1>101 Avenue of the Americas, 10th Floor</com:street1>
          <com:city>New York</com:city>
          <com:stateOrCountry>NY</com:stateOrCountry>
          <com:zipCode>10013</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>341-985-0306</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Yancey L. Spruill</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Former Affiliate</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common Stock</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>J.P. Morgan Securities LLC</name>
          <address>
            <com:street1>390 Madison Avenue</com:street1>
            <com:street2>6th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10017</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>500000</noOfUnitsSold>
        <aggregateMarketValue>18275000</aggregateMarketValue>
        <noOfUnitsOutstanding>90796695</noOfUnitsOutstanding>
        <approxSaleDate>02/27/2024</approxSaleDate>
        <securitiesExchangeName>NYSE</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common Stock</securitiesClassTitle>
        <acquiredDate>02/23/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Transfer</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Yancey Spruill, who acquired the shares from compensation received from the Issuer on 11/15/23</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>500000</amountOfSecuritiesAcquired>
        <paymentDate>02/23/2024</paymentDate>
        <natureOfPayment>N/A</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Yancey L. Spruill Revocable Trust Dated 09/02/20</name>
          <address>
            <com:street1>C/o DigitalOcean Holdings, Inc.</com:street1>
            <com:street2>101 Avenue of the Americas, 10th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10013</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common Stock</securitiesClassTitle>
        <saleDate>02/26/2024</saleDate>
        <amountOfSecuritiesSold>225000</amountOfSecuritiesSold>
        <grossProceeds>8189180</grossProceeds>
      </securitiesSoldInPast3Months>
      <remarks>This filing reflects the covering of 5,000 contracts by the use of 500,000 shares of DigitalOcean control shares.  The shares filed today will be sold by the Yancey L. Spruill Revocable Trust Dated 09/02/20, Yancey L. Spruill as Trustee</remarks>
      <noticeSignature>
        <noticeDate>02/27/2024</noticeDate>
        <signature>/s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Yancey L. Spruill</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
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text: 144
  0001463488
  XXXXXXXX
  LIVE
  0001582961
  DigitalOcean Holdings, Inc.
  001-40252
  101 Avenue of the Americas, 10th Floor
  New York
  NY
  10013
  341-985-0306
  Yancey L. Spruill
  Former Affiliate
  Common Stock
  J.P. Morgan Securities LLC
  390 Madison Avenue
  6th Floor
  New York
  NY
  10017
  500000
  18275000
  90796695
  02/27/2024
  NYSE
  Common Stock
  02/23/2024
  Transfer
  Yancey Spruill, who acquired the shares from compensation received from the Issuer on 11/15/23
  N
  500000
  02/23/2024
  N/A
  N
  Yancey L. Spruill Revocable Trust Dated 09/02/20
  C/o DigitalOcean Holdings, Inc.
  101 Avenue of the Americas, 10th Floor
  New York
  NY
  10013
  Common Stock
  02/26/2024
  225000
  8189180
  This filing reflects the covering of 5,000 contracts by the use of 500,000 shares of DigitalOcean control shares.  The shares filed today will be sold by the Yancey L. Spruill Revocable Trust Dated 09/02/20, Yancey L. Spruill as Trustee
  02/27/2024
  /s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Yancey L. Spruill