content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <!-- Generated by Workiva -->
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001868741</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001861560</issuerCik>
        <issuerName>NUVALENT, INC.</issuerName>
        <secFileNumber>001-40671</secFileNumber>
        <issuerAddress>
          <com:street1>One Broadway, 14th floor</com:street1>
          <com:city>Cambridge</com:city>
          <com:stateOrCountry>MA</com:stateOrCountry>
          <com:zipCode>02142</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>8573577000</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Deborah Miller</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Affiliate</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>J.P. Morgan Securities LLC</name>
          <address>
            <com:street1>360 Madison Avenue - 6th Floor</com:street1>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10017</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>7032</noOfUnitsSold>
        <aggregateMarketValue>273404</aggregateMarketValue>
        <noOfUnitsOutstanding>51269499</noOfUnitsOutstanding>
        <approxSaleDate>05/05/2023</approxSaleDate>
        <securitiesExchangeName>Nasdaq</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Class A Common</securitiesClassTitle>
        <acquiredDate>04/15/2022</acquiredDate>
        <natureOfAcquisitionTransaction>Stock option exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>3032</amountOfSecuritiesAcquired>
        <paymentDate>04/15/2022</paymentDate>
        <natureOfPayment>cash</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Class A Common</securitiesClassTitle>
        <acquiredDate>08/15/2022</acquiredDate>
        <natureOfAcquisitionTransaction>stock option exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>3535</amountOfSecuritiesAcquired>
        <paymentDate>08/15/2022</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Class A Common</securitiesClassTitle>
        <acquiredDate>09/15/2022</acquiredDate>
        <natureOfAcquisitionTransaction>Stock option exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>465</amountOfSecuritiesAcquired>
        <paymentDate>09/15/2022</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Deborah Miller</name>
          <address>
            <com:street1>One Broadway, 14th Floor</com:street1>
            <com:city>Cambridge</com:city>
            <com:stateOrCountry>MA</com:stateOrCountry>
            <com:zipCode>02142</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Class A Common</securitiesClassTitle>
        <saleDate>02/15/2023</saleDate>
        <amountOfSecuritiesSold>2000</amountOfSecuritiesSold>
        <grossProceeds>59124</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Deborah Miller</name>
          <address>
            <com:street1>One Broadway, 14th Floor</com:street1>
            <com:city>Cambridge</com:city>
            <com:stateOrCountry>MA</com:stateOrCountry>
            <com:zipCode>02142</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Class A Common</securitiesClassTitle>
        <saleDate>03/14/2023</saleDate>
        <amountOfSecuritiesSold>2000</amountOfSecuritiesSold>
        <grossProceeds>56501</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Deborah Miller</name>
          <address>
            <com:street1>One Broadway, 14th Floor</com:street1>
            <com:city>Cambridge</com:city>
            <com:stateOrCountry>MA</com:stateOrCountry>
            <com:zipCode>02142</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Class A Common</securitiesClassTitle>
        <saleDate>04/17/2023</saleDate>
        <amountOfSecuritiesSold>2000</amountOfSecuritiesSold>
        <grossProceeds>60206</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Deborah Miller</name>
          <address>
            <com:street1>One Broadway, 14th Floor</com:street1>
            <com:city>Cambridge</com:city>
            <com:stateOrCountry>MA</com:stateOrCountry>
            <com:zipCode>02142</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Class A Common</securitiesClassTitle>
        <saleDate>05/01/2023</saleDate>
        <amountOfSecuritiesSold>900</amountOfSecuritiesSold>
        <grossProceeds>35648</grossProceeds>
      </securitiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>05/05/2023</noticeDate>
        <signature>/s/ JPMS LLC as agent and attorney-in-fact for Deborah Miller</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 6241}]
text: 144
  0001868741
  XXXXXXXX
  LIVE
  0001861560
  NUVALENT, INC.
  001-40671
  One Broadway, 14th floor
  Cambridge
  MA
  02142
  8573577000
  Deborah Miller
  Affiliate
  Common
  J.P. Morgan Securities LLC
  360 Madison Avenue - 6th Floor
  New York
  NY
  10017
  7032
  273404
  51269499
  05/05/2023
  Nasdaq
  Class A Common
  04/15/2022
  Stock option exercise
  Issuer
  N
  3032
  04/15/2022
  cash
  Class A Common
  08/15/2022
  stock option exercise
  Issuer
  N
  3535
  08/15/2022
  Cash
  Class A Common
  09/15/2022
  Stock option exercise
  Issuer
  N
  465
  09/15/2022
  Cash
  N
  Deborah Miller
  One Broadway, 14th Floor
  Cambridge
  MA
  02142
  Class A Common
  02/15/2023
  2000
  59124
  Deborah Miller
  One Broadway, 14th Floor
  Cambridge
  MA
  02142
  Class A Common
  03/14/2023
  2000
  56501
  Deborah Miller
  One Broadway, 14th Floor
  Cambridge
  MA
  02142
  Class A Common
  04/17/2023
  2000
  60206
  Deborah Miller
  One Broadway, 14th Floor
  Cambridge
  MA
  02142
  Class A Common
  05/01/2023
  900
  35648
  05/05/2023
  /s/ JPMS LLC as agent and attorney-in-fact for Deborah Miller