content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001686328</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001823593</issuerCik>
        <issuerName>TuSimple Holdings Inc.</issuerName>
        <secFileNumber>001-40326</secFileNumber>
        <issuerAddress>
          <com:street1>9191 TOWNE CENTRE DRIVE, SUITE 150</com:street1>
          <com:city>SAN DIEGO</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>92122</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>(619) 916-3144</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Tapia Eric R</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Officer</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Class A</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>Fidelity Brokerage Services LLC</name>
          <address>
            <com:street1>900 Salem Street</com:street1>
            <com:city>Smithfield</com:city>
            <com:stateOrCountry>RI</com:stateOrCountry>
            <com:zipCode>02917</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>11119</noOfUnitsSold>
        <aggregateMarketValue>4026.19</aggregateMarketValue>
        <noOfUnitsOutstanding>205890683</noOfUnitsOutstanding>
        <approxSaleDate>01/24/2024</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Class A</securitiesClassTitle>
        <acquiredDate>01/23/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>11119</amountOfSecuritiesAcquired>
        <paymentDate>01/23/2024</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Eric Tapia</name>
          <address>
            <com:street1>9191 Towne Centre Drive</com:street1>
            <com:street2>Suite 150</com:street2>
            <com:city>San Diego</com:city>
            <com:stateOrCountry>CA</com:stateOrCountry>
            <com:zipCode>92122</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Class A</securitiesClassTitle>
        <saleDate>11/02/2023</saleDate>
        <amountOfSecuritiesSold>13550</amountOfSecuritiesSold>
        <grossProceeds>16204.44</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Eric Tapia</name>
          <address>
            <com:street1>9191 Towne Centre Drive</com:street1>
            <com:street2>Suite 150</com:street2>
            <com:city>San Diego</com:city>
            <com:stateOrCountry>CA</com:stateOrCountry>
            <com:zipCode>92122</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Class A</securitiesClassTitle>
        <saleDate>01/23/2024</saleDate>
        <amountOfSecuritiesSold>11943</amountOfSecuritiesSold>
        <grossProceeds>4795.12</grossProceeds>
      </securitiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>01/24/2024</noticeDate>
        <signature>/s/ Gerardo Ibarra-Munoz, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Eric R. Tapia</signature>
      </noticeSignature>
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text: 144
  0001686328
  XXXXXXXX
  LIVE
  0001823593
  TuSimple Holdings Inc.
  001-40326
  9191 TOWNE CENTRE DRIVE, SUITE 150
  SAN DIEGO
  CA
  92122
  (619) 916-3144
  Tapia Eric R
  Officer
  Class A
  Fidelity Brokerage Services LLC
  900 Salem Street
  Smithfield
  RI
  02917
  11119
  4026.19
  205890683
  01/24/2024
  NASDAQ
  Class A
  01/23/2024
  Restricted Stock Vesting
  Issuer
  N
  11119
  01/23/2024
  Compensation
  N
  Eric Tapia
  9191 Towne Centre Drive
  Suite 150
  San Diego
  CA
  92122
  Class A
  11/02/2023
  13550
  16204.44
  Eric Tapia
  9191 Towne Centre Drive
  Suite 150
  San Diego
  CA
  92122
  Class A
  01/23/2024
  11943
  4795.12
  01/24/2024
  /s/ Gerardo Ibarra-Munoz, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Eric R. Tapia