content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001780327</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001755953</issuerCik>
        <issuerName>Akerna Corp.</issuerName>
        <secFileNumber>001-39096</secFileNumber>
        <issuerAddress>
          <com:street1>1550 LARIMER STREET, SUITE 246</com:street1>
          <com:city>DENVER</com:city>
          <com:stateOrCountry>CO</com:stateOrCountry>
          <com:zipCode>80202</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>888-932-6537</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Kane Matthew Ryan</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Director</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>TD Ameritrade</name>
          <address>
            <com:street1>200 S 108th Ave</com:street1>
            <com:city>Omaha</com:city>
            <com:stateOrCountry>NE</com:stateOrCountry>
            <com:zipCode>68154</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>17293</noOfUnitsSold>
        <aggregateMarketValue>4970.00</aggregateMarketValue>
        <noOfUnitsOutstanding>10252018</noOfUnitsOutstanding>
        <approxSaleDate>12/21/2023</approxSaleDate>
        <securitiesExchangeName>Nasdaq</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>12/15/2021</acquiredDate>
        <natureOfAcquisitionTransaction>Purchased</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>17293</amountOfSecuritiesAcquired>
        <paymentDate>12/15/2021</paymentDate>
        <natureOfPayment>N/A</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>charles schwab corp</name>
          <address>
            <com:street1>3000 schwab way</com:street1>
            <com:city>westlake</com:city>
            <com:stateOrCountry>TX</com:stateOrCountry>
            <com:zipCode>76262</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>KERN</securitiesClassTitle>
        <saleDate>12/21/2023</saleDate>
        <amountOfSecuritiesSold>12823</amountOfSecuritiesSold>
        <grossProceeds>3769.00</grossProceeds>
      </securitiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>12/21/2023</noticeDate>
        <signature>Matthew Kane</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 3253}]
text: 144
  0001780327
  XXXXXXXX
  LIVE
  0001755953
  Akerna Corp.
  001-39096
  1550 LARIMER STREET, SUITE 246
  DENVER
  CO
  80202
  888-932-6537
  Kane Matthew Ryan
  Director
  Common
  TD Ameritrade
  200 S 108th Ave
  Omaha
  NE
  68154
  17293
  4970.00
  10252018
  12/21/2023
  Nasdaq
  Common
  12/15/2021
  Purchased
  Issuer
  N
  17293
  12/15/2021
  N/A
  N
  charles schwab corp
  3000 schwab way
  westlake
  TX
  76262
  KERN
  12/21/2023
  12823
  3769.00
  12/21/2023
  Matthew Kane