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        <filer>
          <filerCredentials>
            <cik>0001120477</cik>
            <ccc>XXXXXXXX</ccc>
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        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
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        <issuerCik>0001377121</issuerCik>
        <issuerName>Protagonist Therapeutics, Inc.</issuerName>
        <secFileNumber>001-37852</secFileNumber>
        <issuerAddress>
          <com:street1>7707 Gateway Boulevard, Suite 140</com:street1>
          <com:city>Newark</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94560</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>5104740170</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Dinesh Patel</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>CEO</relationshipToIssuer>
        </relationshipsToIssuer>
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      <securitiesInformation>
        <securitiesClassTitle>Commom</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>J.P. Morgan Securities LLC</name>
          <address>
            <com:street1>390 Madison Avenue</com:street1>
            <com:street2>6th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10017</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>90000</noOfUnitsSold>
        <aggregateMarketValue>2846151</aggregateMarketValue>
        <noOfUnitsOutstanding>58274998</noOfUnitsOutstanding>
        <approxSaleDate>03/01/2024</approxSaleDate>
        <securitiesExchangeName>Nasdaq</securitiesExchangeName>
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        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>02/15/2022</acquiredDate>
        <natureOfAcquisitionTransaction>Compensation</natureOfAcquisitionTransaction>
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        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>9391</amountOfSecuritiesAcquired>
        <paymentDate>02/15/2022</paymentDate>
        <natureOfPayment>NA</natureOfPayment>
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        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>02/18/2019</acquiredDate>
        <natureOfAcquisitionTransaction>Compensation</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>7188</amountOfSecuritiesAcquired>
        <paymentDate>02/18/2019</paymentDate>
        <natureOfPayment>NA</natureOfPayment>
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      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>09/21/2021</acquiredDate>
        <natureOfAcquisitionTransaction>Stock Option Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>30000</amountOfSecuritiesAcquired>
        <paymentDate>09/21/2021</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>03/10/2017</acquiredDate>
        <natureOfAcquisitionTransaction>Stock Option Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>16362</amountOfSecuritiesAcquired>
        <paymentDate>03/10/2017</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>04/06/2017</acquiredDate>
        <natureOfAcquisitionTransaction>Stock Option Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>20000</amountOfSecuritiesAcquired>
        <paymentDate>04/06/2017</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>05/04/2022</acquiredDate>
        <natureOfAcquisitionTransaction>Stock Option Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>7059</amountOfSecuritiesAcquired>
        <paymentDate>05/04/2022</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Dinesh Patel</name>
          <address>
            <com:street1>7707 GATEWAY BLVD., SUITE 140</com:street1>
            <com:city>Newark</com:city>
            <com:stateOrCountry>CA</com:stateOrCountry>
            <com:zipCode>94560</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>12/13/2023</saleDate>
        <amountOfSecuritiesSold>75000</amountOfSecuritiesSold>
        <grossProceeds>1506075</grossProceeds>
      </securitiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>03/01/2024</noticeDate>
        <planAdoptionDates>
          <planAdoptionDate>03/20/2023</planAdoptionDate>
        </planAdoptionDates>
        <signature>/s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Dinesh Patel.</signature>
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text: 144
  0001120477
  XXXXXXXX
  LIVE
  0001377121
  Protagonist Therapeutics, Inc.
  001-37852
  7707 Gateway Boulevard, Suite 140
  Newark
  CA
  94560
  5104740170
  Dinesh Patel
  CEO
  Commom
  J.P. Morgan Securities LLC
  390 Madison Avenue
  6th Floor
  New York
  NY
  10017
  90000
  2846151
  58274998
  03/01/2024
  Nasdaq
  Common
  02/15/2022
  Compensation
  Issuer
  N
  9391
  02/15/2022
  NA
  Common
  02/18/2019
  Compensation
  Issuer
  N
  7188
  02/18/2019
  NA
  Common
  09/21/2021
  Stock Option Exercise
  Issuer
  N
  30000
  09/21/2021
  Cash
  Common
  03/10/2017
  Stock Option Exercise
  Issuer
  N
  16362
  03/10/2017
  Cash
  Common
  04/06/2017
  Stock Option Exercise
  Issuer
  N
  20000
  04/06/2017
  Cash
  Common
  05/04/2022
  Stock Option Exercise
  Issuer
  N
  7059
  05/04/2022
  Cash
  N
  Dinesh Patel
  7707 GATEWAY BLVD., SUITE 140
  Newark
  CA
  94560
  Common
  12/13/2023
  75000
  1506075
  03/01/2024
  03/20/2023
  /s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Dinesh Patel.