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      <filerInfo>
        <filer>
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            <ccc>XXXXXXXX</ccc>
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        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
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        <issuerName>DigitalOcean Holdings, Inc.</issuerName>
        <secFileNumber>001-40252</secFileNumber>
        <issuerAddress>
          <com:street1>101 AVENUE OF THE AMERICAS</com:street1>
          <com:street2>10TH FLOOR</com:street2>
          <com:city>NEW YORK</com:city>
          <com:stateOrCountry>NY</com:stateOrCountry>
          <com:zipCode>10013</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>341-985-0306</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Guy Jeffrey Scott</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Former Officer</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>Fidelity Brokerage Services LLC</name>
          <address>
            <com:street1>900 Salem Street</com:street1>
            <com:city>Smithfield</com:city>
            <com:stateOrCountry>RI</com:stateOrCountry>
            <com:zipCode>02917</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>13253</noOfUnitsSold>
        <aggregateMarketValue>447553.81</aggregateMarketValue>
        <noOfUnitsOutstanding>86008935</noOfUnitsOutstanding>
        <approxSaleDate>01/29/2024</approxSaleDate>
        <securitiesExchangeName>NYSE</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>01/29/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Option Granted - 03/12/2020</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>13253</amountOfSecuritiesAcquired>
        <paymentDate>01/29/2024</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Jeffrey Guy</name>
          <address>
            <com:street1>101 6th Avenue</com:street1>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10013</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>11/10/2023</saleDate>
        <amountOfSecuritiesSold>10000</amountOfSecuritiesSold>
        <grossProceeds>108739.35</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Jeffrey Guy</name>
          <address>
            <com:street1>101 6th Avenue</com:street1>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10013</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>11/17/2023</saleDate>
        <amountOfSecuritiesSold>50000</amountOfSecuritiesSold>
        <grossProceeds>665538.85</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Jeffrey Guy</name>
          <address>
            <com:street1>101 6th Avenue</com:street1>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10013</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>12/06/2023</saleDate>
        <amountOfSecuritiesSold>9871</amountOfSecuritiesSold>
        <grossProceeds>147007.48</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Jeffrey Guy</name>
          <address>
            <com:street1>101 6th Avenue</com:street1>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10013</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>12/11/2023</saleDate>
        <amountOfSecuritiesSold>40129</amountOfSecuritiesSold>
        <grossProceeds>598431.75</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Jeffrey Guy</name>
          <address>
            <com:street1>101 6th Avenue</com:street1>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10013</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>01/26/2024</saleDate>
        <amountOfSecuritiesSold>28747</amountOfSecuritiesSold>
        <grossProceeds>971372.63</grossProceeds>
      </securitiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>01/29/2024</noticeDate>
        <signature>/s/ Emily Navaro, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Jeffrey Guy.</signature>
      </noticeSignature>
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text: 144
  0001845665
  XXXXXXXX
  LIVE
  0001582961
  DigitalOcean Holdings, Inc.
  001-40252
  101 AVENUE OF THE AMERICAS
  10TH FLOOR
  NEW YORK
  NY
  10013
  341-985-0306
  Guy Jeffrey Scott
  Former Officer
  Common
  Fidelity Brokerage Services LLC
  900 Salem Street
  Smithfield
  RI
  02917
  13253
  447553.81
  86008935
  01/29/2024
  NYSE
  Common
  01/29/2024
  Option Granted - 03/12/2020
  Issuer
  N
  13253
  01/29/2024
  Cash
  N
  Jeffrey Guy
  101 6th Avenue
  New York
  NY
  10013
  Common
  11/10/2023
  10000
  108739.35
  Jeffrey Guy
  101 6th Avenue
  New York
  NY
  10013
  Common
  11/17/2023
  50000
  665538.85
  Jeffrey Guy
  101 6th Avenue
  New York
  NY
  10013
  Common
  12/06/2023
  9871
  147007.48
  Jeffrey Guy
  101 6th Avenue
  New York
  NY
  10013
  Common
  12/11/2023
  40129
  598431.75
  Jeffrey Guy
  101 6th Avenue
  New York
  NY
  10013
  Common
  01/26/2024
  28747
  971372.63
  01/29/2024
  /s/ Emily Navaro, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Jeffrey Guy.