content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001354540</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001582961</issuerCik>
        <issuerName>DigitalOcean Holdings, Inc.</issuerName>
        <secFileNumber>001-40252</secFileNumber>
        <issuerAddress>
          <com:street1>101 AVENUE OF THE AMERICAS</com:street1>
          <com:street2>10TH FLOOR</com:street2>
          <com:city>NEW YORK</com:city>
          <com:stateOrCountry>NY</com:stateOrCountry>
          <com:zipCode>10013</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>341-985-0306</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Butte Amy</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Director</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>Fidelity Brokerage Services LLC</name>
          <address>
            <com:street1>900 Salem Street</com:street1>
            <com:city>Smithfield</com:city>
            <com:stateOrCountry>RI</com:stateOrCountry>
            <com:zipCode>02917</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>3760</noOfUnitsSold>
        <aggregateMarketValue>138383.53</aggregateMarketValue>
        <noOfUnitsOutstanding>90796695</noOfUnitsOutstanding>
        <approxSaleDate>02/26/2024</approxSaleDate>
        <securitiesExchangeName>NYSE</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>06/06/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>3760</amountOfSecuritiesAcquired>
        <paymentDate>06/06/2023</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <remarks>Today's trade (February 26, 2023) occurred in the account of Plato Partners, LLC in which Amy Butte Liebowitz is an account stakeholder.
  Amy Butte and Amy Butte Liebowitz are one and the same person.</remarks>
      <noticeSignature>
        <noticeDate>02/26/2024</noticeDate>
        <planAdoptionDates>
          <planAdoptionDate>11/21/2023</planAdoptionDate>
        </planAdoptionDates>
        <signature>/s/ Jodi Disselkamp, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Amy Butte Liebowitz</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 3225}]
text: 144
  0001354540
  XXXXXXXX
  LIVE
  0001582961
  DigitalOcean Holdings, Inc.
  001-40252
  101 AVENUE OF THE AMERICAS
  10TH FLOOR
  NEW YORK
  NY
  10013
  341-985-0306
  Butte Amy
  Director
  Common
  Fidelity Brokerage Services LLC
  900 Salem Street
  Smithfield
  RI
  02917
  3760
  138383.53
  90796695
  02/26/2024
  NYSE
  Common
  06/06/2023
  Restricted Stock Vesting
  Issuer
  N
  3760
  06/06/2023
  Compensation
  Y
  Today's trade (February 26, 2023) occurred in the account of Plato Partners, LLC in which Amy Butte Liebowitz is an account stakeholder.
  Amy Butte and Amy Butte Liebowitz are one and the same person.
  02/26/2024
  11/21/2023
  /s/ Jodi Disselkamp, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Amy Butte Liebowitz