content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001559779</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001162194</issuerCik>
        <issuerName>STANDARD BIOTOOLS INC.</issuerName>
        <secFileNumber>001-34180</secFileNumber>
        <issuerAddress>
          <com:street1>2 TOWER PLACE</com:street1>
          <com:street2>SUITE 2000</com:street2>
          <com:city>SOUTH SAN FRANCISCO</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94080</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>6502666000</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Egholm Michael</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Officer</relationshipToIssuer>
          <relationshipToIssuer>Director</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>Fidelity Brokerage Services LLC</name>
          <address>
            <com:street1>900 Salem Street</com:street1>
            <com:city>Smithfield</com:city>
            <com:stateOrCountry>RI</com:stateOrCountry>
            <com:zipCode>02917</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>5473</noOfUnitsSold>
        <aggregateMarketValue>5151.73</aggregateMarketValue>
        <noOfUnitsOutstanding>379822268</noOfUnitsOutstanding>
        <approxSaleDate>05/23/2025</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Class A</securitiesClassTitle>
        <acquiredDate>05/22/2025</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>5473</amountOfSecuritiesAcquired>
        <paymentDate>05/22/2025</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Michael Egholm</name>
          <address>
            <com:street1>2 Tower Place</com:street1>
            <com:street2>Suite 2000</com:street2>
            <com:city>South San Francisco</com:city>
            <com:stateOrCountry>CA</com:stateOrCountry>
            <com:zipCode>94080</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>05/21/2025</saleDate>
        <amountOfSecuritiesSold>4719</amountOfSecuritiesSold>
        <grossProceeds>4866.70</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Michael Egholm</name>
          <address>
            <com:street1>2 Tower Place</com:street1>
            <com:street2>Suite 2000</com:street2>
            <com:city>South San Francisco</com:city>
            <com:stateOrCountry>CA</com:stateOrCountry>
            <com:zipCode>94080</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>05/22/2025</saleDate>
        <amountOfSecuritiesSold>5116</amountOfSecuritiesSold>
        <grossProceeds>5125.72</grossProceeds>
      </securitiesSoldInPast3Months>
      <remarks>Sale includes an amount necessary to cover a tax obligation resulting from the settlement of a vested equity award distribution.</remarks>
      <noticeSignature>
        <noticeDate>05/23/2025</noticeDate>
        <signature>/s/ Daniel Tucci, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Michael Egholm</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
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text: 144
  0001559779
  XXXXXXXX
  LIVE
  0001162194
  STANDARD BIOTOOLS INC.
  001-34180
  2 TOWER PLACE
  SUITE 2000
  SOUTH SAN FRANCISCO
  CA
  94080
  6502666000
  Egholm Michael
  Officer
  Director
  Common
  Fidelity Brokerage Services LLC
  900 Salem Street
  Smithfield
  RI
  02917
  5473
  5151.73
  379822268
  05/23/2025
  NASDAQ
  Class A
  05/22/2025
  Restricted Stock Vesting
  Issuer
  N
  5473
  05/22/2025
  Compensation
  N
  Michael Egholm
  2 Tower Place
  Suite 2000
  South San Francisco
  CA
  94080
  Common
  05/21/2025
  4719
  4866.70
  Michael Egholm
  2 Tower Place
  Suite 2000
  South San Francisco
  CA
  94080
  Common
  05/22/2025
  5116
  5125.72
  Sale includes an amount necessary to cover a tax obligation resulting from the settlement of a vested equity award distribution.
  05/23/2025
  /s/ Daniel Tucci, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Michael Egholm