content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001611679</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001371285</issuerCik>
        <issuerName>TRUPANION, INC.</issuerName>
        <secFileNumber>001-36537</secFileNumber>
        <issuerAddress>
          <com:street1>6100 4TH AVENUE SOUTH</com:street1>
          <com:street2>SUITE 400</com:street2>
          <com:city>SEATTLE</com:city>
          <com:stateOrCountry>WA</com:stateOrCountry>
          <com:zipCode>98108</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>888-733-2685</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>RAWLINGS DARRYL</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Director</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>FIDELITY BROKERAGE SERVICES LLC</name>
          <address>
            <com:street1>245 SUMMER STREET</com:street1>
            <com:city>Boston</com:city>
            <com:stateOrCountry>MA</com:stateOrCountry>
            <com:zipCode>02210</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>24000</noOfUnitsSold>
        <aggregateMarketValue>1129920.00</aggregateMarketValue>
        <noOfUnitsOutstanding>42490664</noOfUnitsOutstanding>
        <approxSaleDate>05/16/2025</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>04/20/2007</acquiredDate>
        <natureOfAcquisitionTransaction>Founders Shares</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>n/a</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>24000</amountOfSecuritiesAcquired>
        <paymentDate>04/20/2007</paymentDate>
        <natureOfPayment>n/a</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Montgomery Holdings Trust </name>
          <address>
            <com:street1>6100 4th Avenue,S suite 200</com:street1>
            <com:city>Seattle</com:city>
            <com:stateOrCountry>WA</com:stateOrCountry>
            <com:zipCode>98108</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>TRUP</securitiesClassTitle>
        <saleDate>03/05/2025</saleDate>
        <amountOfSecuritiesSold>2000</amountOfSecuritiesSold>
        <grossProceeds>67348.12</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Wilson Holding Trust </name>
          <address>
            <com:street1>6100 4th Avenue,S suite 200,</com:street1>
            <com:city>Seattle </com:city>
            <com:stateOrCountry>WA</com:stateOrCountry>
            <com:zipCode>98108</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>TRUP</securitiesClassTitle>
        <saleDate>03/05/2025</saleDate>
        <amountOfSecuritiesSold>2000</amountOfSecuritiesSold>
        <grossProceeds>67198.33</grossProceeds>
      </securitiesSoldInPast3Months>
      <remarks>12,000 shares sold from Montgomery Holdings Trust and 12,000 shares sold from Wilson Holding Trust where Joseph Tappan is signing as trustee but form 144 will be filed under Darryl Rawlings credentials since children are underage and live at home.</remarks>
      <noticeSignature>
        <noticeDate>05/16/2025</noticeDate>
        <signature>Carmen Zamores as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for RAWLINGS DARRYL</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 4318}]
text: 144
  0001611679
  XXXXXXXX
  LIVE
  0001371285
  TRUPANION, INC.
  001-36537
  6100 4TH AVENUE SOUTH
  SUITE 400
  SEATTLE
  WA
  98108
  888-733-2685
  RAWLINGS DARRYL
  Director
  Common
  FIDELITY BROKERAGE SERVICES LLC
  245 SUMMER STREET
  Boston
  MA
  02210
  24000
  1129920.00
  42490664
  05/16/2025
  NASDAQ
  Common
  04/20/2007
  Founders Shares
  n/a
  N
  24000
  04/20/2007
  n/a
  N
  Montgomery Holdings Trust
  6100 4th Avenue,S suite 200
  Seattle
  WA
  98108
  TRUP
  03/05/2025
  2000
  67348.12
  Wilson Holding Trust
  6100 4th Avenue,S suite 200,
  Seattle
  WA
  98108
  TRUP
  03/05/2025
  2000
  67198.33
  12,000 shares sold from Montgomery Holdings Trust and 12,000 shares sold from Wilson Holding Trust where Joseph Tappan is signing as trustee but form 144 will be filed under Darryl Rawlings credentials since children are underage and live at home.
  05/16/2025
  Carmen Zamores as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for RAWLINGS DARRYL