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      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001950760</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001805526</issuerCik>
        <issuerName>DeFi Development Corp.</issuerName>
        <secFileNumber>001-41748</secFileNumber>
        <issuerAddress>
          <com:street1>6401 CONGRESS AVE</com:street1>
          <com:street2>STE 250</com:street2>
          <com:city>BOCA RATON</com:city>
          <com:stateOrCountry>FL</com:stateOrCountry>
          <com:zipCode>33487</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>5615594111</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>LEMOS MARCELO</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Associated to Affiliate</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>Fidelity Brokerage Services LLC</name>
          <address>
            <com:street1>245 Summer Street</com:street1>
            <com:city>Boston</com:city>
            <com:stateOrCountry>MA</com:stateOrCountry>
            <com:zipCode>02110</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>5243</noOfUnitsSold>
        <aggregateMarketValue>228332.00</aggregateMarketValue>
        <noOfUnitsOutstanding>2066711</noOfUnitsOutstanding>
        <approxSaleDate>05/23/2025</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>11/29/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Open Market Purchase</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>380</amountOfSecuritiesAcquired>
        <paymentDate>11/29/2023</paymentDate>
        <natureOfPayment>Cash/Check</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>11/30/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Open Market Purchase</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>368</amountOfSecuritiesAcquired>
        <paymentDate>11/30/2023</paymentDate>
        <natureOfPayment>Cash/Check</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>12/04/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Open Market Purchase</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>619</amountOfSecuritiesAcquired>
        <paymentDate>12/04/2023</paymentDate>
        <natureOfPayment>Cash/Check</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>12/12/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Transferred from Holding Co.</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>3876</amountOfSecuritiesAcquired>
        <paymentDate>12/12/2024</paymentDate>
        <natureOfPayment>N/A</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Marcelo Edgardo Lemos</name>
          <address>
            <com:street1>6401 Congress Ave</com:street1>
            <com:street2>Suite 250</com:street2>
            <com:city>Boca Raton</com:city>
            <com:stateOrCountry>FL</com:stateOrCountry>
            <com:zipCode>33487</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Defi Development</securitiesClassTitle>
        <saleDate>05/20/2025</saleDate>
        <amountOfSecuritiesSold>525</amountOfSecuritiesSold>
        <grossProceeds>95319.14</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name> Marcelo Edgardo Lemos</name>
          <address>
            <com:street1>6401 Congress Ave</com:street1>
            <com:street2>Suite 250</com:street2>
            <com:city>Boca Raton</com:city>
            <com:stateOrCountry>FL</com:stateOrCountry>
            <com:zipCode>33487</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Defi Development</securitiesClassTitle>
        <saleDate>05/21/2025</saleDate>
        <amountOfSecuritiesSold>7000</amountOfSecuritiesSold>
        <grossProceeds>208039.54</grossProceeds>
      </securitiesSoldInPast3Months>
      <remarks>Shares sold by Gustavo Nicolas Lemos Moure</remarks>
      <noticeSignature>
        <noticeDate>05/23/2025</noticeDate>
        <signature>/s/ Christian Nasser as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Marcelo Lemos</signature>
      </noticeSignature>
    </formData>
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text: 144
  0001950760
  XXXXXXXX
  LIVE
  0001805526
  DeFi Development Corp.
  001-41748
  6401 CONGRESS AVE
  STE 250
  BOCA RATON
  FL
  33487
  5615594111
  LEMOS MARCELO
  Associated to Affiliate
  Common
  Fidelity Brokerage Services LLC
  245 Summer Street
  Boston
  MA
  02110
  5243
  228332.00
  2066711
  05/23/2025
  NASDAQ
  Common
  11/29/2023
  Open Market Purchase
  Issuer
  N
  380
  11/29/2023
  Cash/Check
  Common
  11/30/2023
  Open Market Purchase
  Issuer
  N
  368
  11/30/2023
  Cash/Check
  Common
  12/04/2023
  Open Market Purchase
  Issuer
  N
  619
  12/04/2023
  Cash/Check
  Common
  12/12/2024
  Transferred from Holding Co.
  Issuer
  N
  3876
  12/12/2024
  N/A
  N
  Marcelo Edgardo Lemos
  6401 Congress Ave
  Suite 250
  Boca Raton
  FL
  33487
  Defi Development
  05/20/2025
  525
  95319.14
  Marcelo Edgardo Lemos
  6401 Congress Ave
  Suite 250
  Boca Raton
  FL
  33487
  Defi Development
  05/21/2025
  7000
  208039.54
  Shares sold by Gustavo Nicolas Lemos Moure
  05/23/2025
  /s/ Christian Nasser as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Marcelo Lemos