content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <!-- Generated by Workiva -->
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001335061</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001110611</issuerCik>
        <issuerName>ON24, Inc.</issuerName>
        <secFileNumber>001-39965</secFileNumber>
        <issuerAddress>
          <com:street1>50 Beale Street</com:street1>
          <com:street2>8th Floor</com:street2>
          <com:city>San Francisco</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94105</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>1-415-369-8000</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Steven Vattuone</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Officer</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>J.P. Morgan Securities LLC</name>
          <address>
            <com:street1>390 Madison Avenue</com:street1>
            <com:street2>6th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10017</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>27522</noOfUnitsSold>
        <aggregateMarketValue>189351</aggregateMarketValue>
        <noOfUnitsOutstanding>43904608</noOfUnitsOutstanding>
        <approxSaleDate>08/21/2023</approxSaleDate>
        <securitiesExchangeName>NYSE</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>08/20/2023</acquiredDate>
        <natureOfAcquisitionTransaction>RSU Vest</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>27522</amountOfSecuritiesAcquired>
        <paymentDate>08/20/2023</paymentDate>
        <natureOfPayment>NA</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Steven Vattuone</name>
          <address>
            <com:street1>50 Beale Street, 8th Floor</com:street1>
            <com:city>San Francisco</com:city>
            <com:stateOrCountry>CA</com:stateOrCountry>
            <com:zipCode>94105</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>05/30/2023</saleDate>
        <amountOfSecuritiesSold>1001</amountOfSecuritiesSold>
        <grossProceeds>7924</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Steven Vattuone</name>
          <address>
            <com:street1>50 Beale Street, 8th Floor</com:street1>
            <com:city>San Francisco</com:city>
            <com:stateOrCountry>CA</com:stateOrCountry>
            <com:zipCode>94105</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>05/31/2023</saleDate>
        <amountOfSecuritiesSold>32041</amountOfSecuritiesSold>
        <grossProceeds>254345</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Steven Vattuone</name>
          <address>
            <com:street1>50 Beale Street, 8th Floor</com:street1>
            <com:city>San Francisco</com:city>
            <com:stateOrCountry>CA</com:stateOrCountry>
            <com:zipCode>94105</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>06/01/2023</saleDate>
        <amountOfSecuritiesSold>9025</amountOfSecuritiesSold>
        <grossProceeds>72109</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Steven Vattuone</name>
          <address>
            <com:street1>50 Beale Street, 8th Floor</com:street1>
            <com:city>San Francisco</com:city>
            <com:stateOrCountry>CA</com:stateOrCountry>
            <com:zipCode>94105</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>06/02/2023</saleDate>
        <amountOfSecuritiesSold>14249</amountOfSecuritiesSold>
        <grossProceeds>112769</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Steven Vattuone</name>
          <address>
            <com:street1>50 Beale Street, 8th Floor</com:street1>
            <com:city>San Francisco</com:city>
            <com:stateOrCountry>CA</com:stateOrCountry>
            <com:zipCode>94105</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>07/13/2023</saleDate>
        <amountOfSecuritiesSold>2000</amountOfSecuritiesSold>
        <grossProceeds>17826</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Steven Vattuone</name>
          <address>
            <com:street1>50 Beale Street, 8th Floor</com:street1>
            <com:city>San Francisco</com:city>
            <com:stateOrCountry>CA</com:stateOrCountry>
            <com:zipCode>94105</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>07/14/2023</saleDate>
        <amountOfSecuritiesSold>200</amountOfSecuritiesSold>
        <grossProceeds>1782</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Steven Vattuone</name>
          <address>
            <com:street1>50 Beale Street, 8th Floor</com:street1>
            <com:city>San Francisco</com:city>
            <com:stateOrCountry>CA</com:stateOrCountry>
            <com:zipCode>94105</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>07/31/2023</saleDate>
        <amountOfSecuritiesSold>6722</amountOfSecuritiesSold>
        <grossProceeds>59960</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Steven Vattuone</name>
          <address>
            <com:street1>50 Beale Street, 8th Floor</com:street1>
            <com:city>San Francisco</com:city>
            <com:stateOrCountry>CA</com:stateOrCountry>
            <com:zipCode>94105</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>08/01/2023</saleDate>
        <amountOfSecuritiesSold>39223</amountOfSecuritiesSold>
        <grossProceeds>351485</grossProceeds>
      </securitiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>08/21/2023</noticeDate>
        <planAdoptionDates>
          <planAdoptionDate>09/13/2022</planAdoptionDate>
        </planAdoptionDates>
        <signature>/s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Steven Vattuone</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 7723}]
text: 144
  0001335061
  XXXXXXXX
  LIVE
  0001110611
  ON24, Inc.
  001-39965
  50 Beale Street
  8th Floor
  San Francisco
  CA
  94105
  1-415-369-8000
  Steven Vattuone
  Officer
  Common
  J.P. Morgan Securities LLC
  390 Madison Avenue
  6th Floor
  New York
  NY
  10017
  27522
  189351
  43904608
  08/21/2023
  NYSE
  Common
  08/20/2023
  RSU Vest
  Issuer
  N
  27522
  08/20/2023
  NA
  N
  Steven Vattuone
  50 Beale Street, 8th Floor
  San Francisco
  CA
  94105
  Common
  05/30/2023
  1001
  7924
  Steven Vattuone
  50 Beale Street, 8th Floor
  San Francisco
  CA
  94105
  Common
  05/31/2023
  32041
  254345
  Steven Vattuone
  50 Beale Street, 8th Floor
  San Francisco
  CA
  94105
  Common
  06/01/2023
  9025
  72109
  Steven Vattuone
  50 Beale Street, 8th Floor
  San Francisco
  CA
  94105
  Common
  06/02/2023
  14249
  112769
  Steven Vattuone
  50 Beale Street, 8th Floor
  San Francisco
  CA
  94105
  Common
  07/13/2023
  2000
  17826
  Steven Vattuone
  50 Beale Street, 8th Floor
  San Francisco
  CA
  94105
  Common
  07/14/2023
  200
  1782
  Steven Vattuone
  50 Beale Street, 8th Floor
  San Francisco
  CA
  94105
  Common
  07/31/2023
  6722
  59960
  Steven Vattuone
  50 Beale Street, 8th Floor
  San Francisco
  CA
  94105
  Common
  08/01/2023
  39223
  351485
  08/21/2023
  09/13/2022
  /s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Steven Vattuone