content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001983944</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001761612</issuerCik>
        <issuerName>BICYCLE THERAPEUTICS PLC</issuerName>
        <secFileNumber>001-38916</secFileNumber>
        <issuerAddress>
          <com:street1>BLOCKS A &amp; B, PORTWAY BUILDING</com:street1>
          <com:street2>GRANTA PARK, GREAT ABINGTON</com:street2>
          <com:city>CAMBRIDGE</com:city>
          <com:stateOrCountry>X0</com:stateOrCountry>
          <com:zipCode>CB21 6GS</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>011441223261503</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Thompson Travis Alvin</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Officer</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>American Depositary Shares</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>Fidelity Brokerage Services LLC</name>
          <address>
            <com:street1>900 Salem Street</com:street1>
            <com:city>Smithfield</com:city>
            <com:stateOrCountry>RI</com:stateOrCountry>
            <com:zipCode>02917</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>2686</noOfUnitsSold>
        <aggregateMarketValue>40290.00</aggregateMarketValue>
        <noOfUnitsOutstanding>47553922</noOfUnitsOutstanding>
        <approxSaleDate>01/06/2025</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>American Depositary Shares</securitiesClassTitle>
        <acquiredDate>01/03/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>754</amountOfSecuritiesAcquired>
        <paymentDate>01/03/2023</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>American Depositary Shares</securitiesClassTitle>
        <acquiredDate>04/03/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>226</amountOfSecuritiesAcquired>
        <paymentDate>04/03/2023</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>American Depositary Shares</securitiesClassTitle>
        <acquiredDate>07/03/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>220</amountOfSecuritiesAcquired>
        <paymentDate>07/03/2023</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>American Depositary Shares</securitiesClassTitle>
        <acquiredDate>10/03/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>227</amountOfSecuritiesAcquired>
        <paymentDate>10/03/2023</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>American Depositary Shares</securitiesClassTitle>
        <acquiredDate>01/03/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>928</amountOfSecuritiesAcquired>
        <paymentDate>01/03/2024</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>American Depositary Shares</securitiesClassTitle>
        <acquiredDate>04/03/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>331</amountOfSecuritiesAcquired>
        <paymentDate>04/03/2024</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Travis Thompson</name>
          <address>
            <com:street1>Blocks A &amp; B, Portway Building</com:street1>
            <com:street2>Granta Park, Great Abington</com:street2>
            <com:city>Cambridge</com:city>
            <com:stateOrCountry>X0</com:stateOrCountry>
            <com:zipCode>CB21 6GS</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>American Depositary Shares</securitiesClassTitle>
        <saleDate>11/11/2024</saleDate>
        <amountOfSecuritiesSold>6256</amountOfSecuritiesSold>
        <grossProceeds>157112.04</grossProceeds>
      </securitiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>01/06/2025</noticeDate>
        <planAdoptionDates>
          <planAdoptionDate>08/13/2024</planAdoptionDate>
        </planAdoptionDates>
        <signature>/s/Wade Moss, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Travis Thompson</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 6545}]
text: 144
  0001983944
  XXXXXXXX
  LIVE
  0001761612
  BICYCLE THERAPEUTICS PLC
  001-38916
  BLOCKS A & B, PORTWAY BUILDING
  GRANTA PARK, GREAT ABINGTON
  CAMBRIDGE
  X0
  CB21 6GS
  011441223261503
  Thompson Travis Alvin
  Officer
  American Depositary Shares
  Fidelity Brokerage Services LLC
  900 Salem Street
  Smithfield
  RI
  02917
  2686
  40290.00
  47553922
  01/06/2025
  NASDAQ
  American Depositary Shares
  01/03/2023
  Restricted Stock Vesting
  Issuer
  N
  754
  01/03/2023
  Compensation
  American Depositary Shares
  04/03/2023
  Restricted Stock Vesting
  Issuer
  N
  226
  04/03/2023
  Compensation
  American Depositary Shares
  07/03/2023
  Restricted Stock Vesting
  Issuer
  N
  220
  07/03/2023
  Compensation
  American Depositary Shares
  10/03/2023
  Restricted Stock Vesting
  Issuer
  N
  227
  10/03/2023
  Compensation
  American Depositary Shares
  01/03/2024
  Restricted Stock Vesting
  Issuer
  N
  928
  01/03/2024
  Compensation
  American Depositary Shares
  04/03/2024
  Restricted Stock Vesting
  Issuer
  N
  331
  04/03/2024
  Compensation
  N
  Travis Thompson
  Blocks A & B, Portway Building
  Granta Park, Great Abington
  Cambridge
  X0
  CB21 6GS
  American Depositary Shares
  11/11/2024
  6256
  157112.04
  01/06/2025
  08/13/2024
  /s/Wade Moss, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Travis Thompson