content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001640560</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001126234</issuerCik>
        <issuerName>LUMOS PHARMA, INC.</issuerName>
        <secFileNumber>001-35342</secFileNumber>
        <issuerAddress>
          <com:street1>4200 MARATHON BLVD.</com:street1>
          <com:street2>SUITE 200</com:street2>
          <com:city>AUSTIN</com:city>
          <com:stateOrCountry>MN</com:stateOrCountry>
          <com:zipCode>78756</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>512-215-2630</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>McKew John C.</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Officer</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>Piper Sandler &amp; Co.</name>
          <address>
            <com:street1>Nicollet Mall Ste 900</com:street1>
            <com:city>Minneapolis</com:city>
            <com:stateOrCountry>MN</com:stateOrCountry>
            <com:zipCode>55402</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>3407</noOfUnitsSold>
        <aggregateMarketValue>11174.96</aggregateMarketValue>
        <noOfUnitsOutstanding>8170000</noOfUnitsOutstanding>
        <approxSaleDate>08/02/2023</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>common</securitiesClassTitle>
        <acquiredDate>08/01/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Vested RSU shares</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>3407</amountOfSecuritiesAcquired>
        <paymentDate>08/01/2023</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>08/02/2023</noticeDate>
        <planAdoptionDates>
          <planAdoptionDate>06/03/2022</planAdoptionDate>
        </planAdoptionDates>
        <signature>/s/ Linda Moran, as a duly authorized representative of Piper Sandler &amp; Co Corporate &amp; Venture Services FA, as attorney-in-fact for John McKew</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 2991}]
text: 144
  0001640560
  XXXXXXXX
  LIVE
  0001126234
  LUMOS PHARMA, INC.
  001-35342
  4200 MARATHON BLVD.
  SUITE 200
  AUSTIN
  MN
  78756
  512-215-2630
  McKew John C.
  Officer
  Common
  Piper Sandler & Co.
  Nicollet Mall Ste 900
  Minneapolis
  MN
  55402
  3407
  11174.96
  8170000
  08/02/2023
  NASDAQ
  common
  08/01/2023
  Vested RSU shares
  Issuer
  N
  3407
  08/01/2023
  Compensation
  Y
  08/02/2023
  06/03/2022
  /s/ Linda Moran, as a duly authorized representative of Piper Sandler & Co Corporate & Venture Services FA, as attorney-in-fact for John McKew