content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <!-- Generated by Workiva -->
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001608936</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001650648</issuerCik>
        <issuerName>4D Molecular Therapeutics Inc.</issuerName>
        <secFileNumber>001-39782</secFileNumber>
        <issuerAddress>
          <com:street1>5858 Horton Street #455</com:street1>
          <com:city>Emeryville</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94608</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>9785520900</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Jacob Chacko</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Affiliate</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>JPMorgan Securies LLC</name>
          <address>
            <com:street1>390 Madison Ave</com:street1>
            <com:street2>6th Floor</com:street2>
            <com:city>NY</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10019</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>15000</noOfUnitsSold>
        <aggregateMarketValue>297300</aggregateMarketValue>
        <noOfUnitsOutstanding>32196219</noOfUnitsOutstanding>
        <approxSaleDate>04/21/2023</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>03/20/2019</acquiredDate>
        <natureOfAcquisitionTransaction>Stock Option Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>15000</amountOfSecuritiesAcquired>
        <paymentDate>03/20/2019</paymentDate>
        <natureOfPayment>cash</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>04/21/2023</noticeDate>
        <planAdoptionDates>
          <planAdoptionDate>12/28/2022</planAdoptionDate>
        </planAdoptionDates>
        <signature>/s/ JPMS LLC as agent and attorney-in-fact forJacob Chacko</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 2932}]
text: 144
  0001608936
  XXXXXXXX
  LIVE
  0001650648
  4D Molecular Therapeutics Inc.
  001-39782
  5858 Horton Street #455
  Emeryville
  CA
  94608
  9785520900
  Jacob Chacko
  Affiliate
  Common
  JPMorgan Securies LLC
  390 Madison Ave
  6th Floor
  NY
  NY
  10019
  15000
  297300
  32196219
  04/21/2023
  NASDAQ
  Common
  03/20/2019
  Stock Option Exercise
  Issuer
  N
  15000
  03/20/2019
  cash
  Y
  04/21/2023
  12/28/2022
  /s/ JPMS LLC as agent and attorney-in-fact forJacob Chacko