content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <!-- Generated by Workiva -->
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001841671</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001110611</issuerCik>
        <issuerName>ON24, Inc.</issuerName>
        <secFileNumber>001-39965</secFileNumber>
        <issuerAddress>
          <com:street1>50 Beale Street, 8th Floor</com:street1>
          <com:city>San Francisco</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94105</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>4153698000</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>James Blackie</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Affilate</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>J.P. Morgan Securities LLC</name>
          <address>
            <com:street1>390 Madison Avenue</com:street1>
            <com:street2>6th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10017</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>30000</noOfUnitsSold>
        <aggregateMarketValue>257700</aggregateMarketValue>
        <noOfUnitsOutstanding>45864254</noOfUnitsOutstanding>
        <approxSaleDate>06/14/2023</approxSaleDate>
        <securitiesExchangeName>NYSE</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>03/15/2023</acquiredDate>
        <natureOfAcquisitionTransaction>RSU Vest</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>10000</amountOfSecuritiesAcquired>
        <paymentDate>03/15/2023</paymentDate>
        <natureOfPayment>NA</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>06/14/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Stock Option Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>20000</amountOfSecuritiesAcquired>
        <paymentDate>06/14/2023</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>James Blackie</name>
          <address>
            <com:street1>50 Beale Street, 8th Floor</com:street1>
            <com:city>San Francisco</com:city>
            <com:stateOrCountry>CA</com:stateOrCountry>
            <com:zipCode>94105</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>06/01/2023</saleDate>
        <amountOfSecuritiesSold>8711</amountOfSecuritiesSold>
        <grossProceeds>68178</grossProceeds>
      </securitiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>06/14/2023</noticeDate>
        <planAdoptionDates>
          <planAdoptionDate>03/14/2023</planAdoptionDate>
        </planAdoptionDates>
        <signature>/s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for James Blackie</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 4054}]
text: 144
  0001841671
  XXXXXXXX
  LIVE
  0001110611
  ON24, Inc.
  001-39965
  50 Beale Street, 8th Floor
  San Francisco
  CA
  94105
  4153698000
  James Blackie
  Affilate
  Common
  J.P. Morgan Securities LLC
  390 Madison Avenue
  6th Floor
  New York
  NY
  10017
  30000
  257700
  45864254
  06/14/2023
  NYSE
  Common
  03/15/2023
  RSU Vest
  Issuer
  N
  10000
  03/15/2023
  NA
  Common
  06/14/2023
  Stock Option Exercise
  Issuer
  N
  20000
  06/14/2023
  Cash
  N
  James Blackie
  50 Beale Street, 8th Floor
  San Francisco
  CA
  94105
  Common
  06/01/2023
  8711
  68178
  06/14/2023
  03/14/2023
  /s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for James Blackie