content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <!-- Generated by Workiva -->
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001535065</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001850906</issuerCik>
        <issuerName>Singular Genomics Systems, Inc.</issuerName>
        <secFileNumber>001-40443</secFileNumber>
        <issuerAddress>
          <com:street1>3010 Science Park Road</com:street1>
          <com:city>San Diego</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>92121</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>8583337830</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Kim Kamdar</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Affiliate</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>J.P. Morgan Securities LLC</name>
          <address>
            <com:street1>390 Madison Avenue</com:street1>
            <com:street2>6th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10017</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>77991</noOfUnitsSold>
        <aggregateMarketValue>44424</aggregateMarketValue>
        <noOfUnitsOutstanding>73125481</noOfUnitsOutstanding>
        <approxSaleDate>08/11/2023</approxSaleDate>
        <securitiesExchangeName>Nasdaq</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>09/20/2016</acquiredDate>
        <natureOfAcquisitionTransaction>Investment in Singular as a private company</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>2666781</amountOfSecuritiesAcquired>
        <paymentDate>09/20/2016</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Kim Kamdar</name>
          <address>
            <com:street1>3010 Science Park Road</com:street1>
            <com:city>San Diego</com:city>
            <com:stateOrCountry>CA</com:stateOrCountry>
            <com:zipCode>92121</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>08/11/2023</saleDate>
        <amountOfSecuritiesSold>353657</amountOfSecuritiesSold>
        <grossProceeds>210603</grossProceeds>
      </securitiesSoldInPast3Months>
      <remarks>The shares filed today will be sold by Domain Partners IX LP , Kim Kamdar  as Partner</remarks>
      <noticeSignature>
        <noticeDate>08/14/2023</noticeDate>
        <signature>/s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Kim Kamdar</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 3566}]
text: 144
  0001535065
  XXXXXXXX
  LIVE
  0001850906
  Singular Genomics Systems, Inc.
  001-40443
  3010 Science Park Road
  San Diego
  CA
  92121
  8583337830
  Kim Kamdar
  Affiliate
  Common
  J.P. Morgan Securities LLC
  390 Madison Avenue
  6th Floor
  New York
  NY
  10017
  77991
  44424
  73125481
  08/11/2023
  Nasdaq
  Common
  09/20/2016
  Investment in Singular as a private company
  Issuer
  N
  2666781
  09/20/2016
  Cash
  N
  Kim Kamdar
  3010 Science Park Road
  San Diego
  CA
  92121
  Common
  08/11/2023
  353657
  210603
  The shares filed today will be sold by Domain Partners IX LP , Kim Kamdar  as Partner
  08/14/2023
  /s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Kim Kamdar