content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001611679</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001371285</issuerCik>
        <issuerName>TRUPANION, INC.</issuerName>
        <secFileNumber>001-36537</secFileNumber>
        <issuerAddress>
          <com:street1>6100 4TH AVENUE SOUTH</com:street1>
          <com:street2>SUITE 400</com:street2>
          <com:city>SEATTLE</com:city>
          <com:stateOrCountry>WA</com:stateOrCountry>
          <com:zipCode>98108</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>888-733-2685</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>RAWLINGS DARRYL</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Director</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common Stock</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>Fidelity Brokerage Services LLC</name>
          <address>
            <com:street1>245 Summer Street</com:street1>
            <com:city>Boston</com:city>
            <com:stateOrCountry>MA</com:stateOrCountry>
            <com:zipCode>02110</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>4000</noOfUnitsSold>
        <aggregateMarketValue>134400.40</aggregateMarketValue>
        <noOfUnitsOutstanding>42343514</noOfUnitsOutstanding>
        <approxSaleDate>03/05/2025</approxSaleDate>
        <securitiesExchangeName>nasdaq</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>03/09/2015</acquiredDate>
        <natureOfAcquisitionTransaction>Founder Shares</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>N/A</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>30000</amountOfSecuritiesAcquired>
        <paymentDate>03/09/2015</paymentDate>
        <natureOfPayment>N/A</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>04/20/2007</acquiredDate>
        <natureOfAcquisitionTransaction>Founder Shares</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>N/A</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>30000</amountOfSecuritiesAcquired>
        <paymentDate>04/20/2007</paymentDate>
        <natureOfPayment>N/A</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <remarks>shares are being sold from below accounts
  2,000 shares from MONTGOMERY HOLDINGS TRUST
  2,000 shares from WILSON HOLDINGS TRUST</remarks>
      <noticeSignature>
        <noticeDate>03/05/2025</noticeDate>
        <signature>/s/ Audrey Skillern as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for DARRYL RAWLINGS</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 3524}]
text: 144
  0001611679
  XXXXXXXX
  LIVE
  0001371285
  TRUPANION, INC.
  001-36537
  6100 4TH AVENUE SOUTH
  SUITE 400
  SEATTLE
  WA
  98108
  888-733-2685
  RAWLINGS DARRYL
  Director
  Common Stock
  Fidelity Brokerage Services LLC
  245 Summer Street
  Boston
  MA
  02110
  4000
  134400.40
  42343514
  03/05/2025
  nasdaq
  Common
  03/09/2015
  Founder Shares
  N/A
  N
  30000
  03/09/2015
  N/A
  Common
  04/20/2007
  Founder Shares
  N/A
  N
  30000
  04/20/2007
  N/A
  Y
  shares are being sold from below accounts
  2,000 shares from MONTGOMERY HOLDINGS TRUST
  2,000 shares from WILSON HOLDINGS TRUST
  03/05/2025
  /s/ Audrey Skillern as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for DARRYL RAWLINGS