content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <!-- Generated by Workiva -->
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001673762</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001371285</issuerCik>
        <issuerName>Trupanion Inc</issuerName>
        <secFileNumber>001-36537</secFileNumber>
        <issuerAddress>
          <com:street1>6100 4th Avenue S, Suite 400</com:street1>
          <com:city>Seattle</com:city>
          <com:stateOrCountry>WA</com:stateOrCountry>
          <com:zipCode>98108</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>855-727-9079</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Tricia Plouf</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Affiliate</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>JP Morgan Securities LLC</name>
          <address>
            <com:street1>390 Madison Avenue</com:street1>
            <com:street2>6th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10019</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>1050</noOfUnitsSold>
        <aggregateMarketValue>46063</aggregateMarketValue>
        <noOfUnitsOutstanding>41020005</noOfUnitsOutstanding>
        <approxSaleDate>04/12/2023</approxSaleDate>
        <securitiesExchangeName>Nasdaq</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>08/25/2022</acquiredDate>
        <natureOfAcquisitionTransaction>RSU Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>1050</amountOfSecuritiesAcquired>
        <paymentDate>08/25/2022</paymentDate>
        <natureOfPayment>N/A</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Tricia Plouf</name>
          <address>
            <com:street1>care of Trupanion Inc</com:street1>
            <com:street2>6100 4th Avenue S, Suite 400</com:street2>
            <com:city>Seattle</com:city>
            <com:stateOrCountry>WA</com:stateOrCountry>
            <com:zipCode>98108</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>01/31/2023</saleDate>
        <amountOfSecuritiesSold>350</amountOfSecuritiesSold>
        <grossProceeds>20659.79</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Tricia Plouf</name>
          <address>
            <com:street1>care of Trupanion Inc</com:street1>
            <com:street2>6100 4th Avenue S, Suite 400</com:street2>
            <com:city>Seattle</com:city>
            <com:stateOrCountry>WA</com:stateOrCountry>
            <com:zipCode>98108</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>02/08/2023</saleDate>
        <amountOfSecuritiesSold>700</amountOfSecuritiesSold>
        <grossProceeds>41829.23</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Tricia Plouf</name>
          <address>
            <com:street1>care of Trupanion Inc</com:street1>
            <com:street2>6100 4th Avenue S, Suite 400</com:street2>
            <com:city>Seattle</com:city>
            <com:stateOrCountry>WA</com:stateOrCountry>
            <com:zipCode>98108</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>03/08/2023</saleDate>
        <amountOfSecuritiesSold>350</amountOfSecuritiesSold>
        <grossProceeds>20164.11</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Tricia Plouf</name>
          <address>
            <com:street1>care of Trupanion Inc</com:street1>
            <com:street2>6100 4th Avenue Suite 400</com:street2>
            <com:city>Seattle</com:city>
            <com:stateOrCountry>WA</com:stateOrCountry>
            <com:zipCode>98108</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <saleDate>03/21/2023</saleDate>
        <amountOfSecuritiesSold>350</amountOfSecuritiesSold>
        <grossProceeds>20878.51</grossProceeds>
      </securitiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>04/12/2023</noticeDate>
        <planAdoptionDates>
          <planAdoptionDate>11/29/2022</planAdoptionDate>
        </planAdoptionDates>
        <signature>/s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Tricia Plouf</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
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text: 144
  0001673762
  XXXXXXXX
  LIVE
  0001371285
  Trupanion Inc
  001-36537
  6100 4th Avenue S, Suite 400
  Seattle
  WA
  98108
  855-727-9079
  Tricia Plouf
  Affiliate
  Common
  JP Morgan Securities LLC
  390 Madison Avenue
  6th Floor
  New York
  NY
  10019
  1050
  46063
  41020005
  04/12/2023
  Nasdaq
  Common
  08/25/2022
  RSU Vesting
  Issuer
  N
  1050
  08/25/2022
  N/A
  N
  Tricia Plouf
  care of Trupanion Inc
  6100 4th Avenue S, Suite 400
  Seattle
  WA
  98108
  Common
  01/31/2023
  350
  20659.79
  Tricia Plouf
  care of Trupanion Inc
  6100 4th Avenue S, Suite 400
  Seattle
  WA
  98108
  Common
  02/08/2023
  700
  41829.23
  Tricia Plouf
  care of Trupanion Inc
  6100 4th Avenue S, Suite 400
  Seattle
  WA
  98108
  Common
  03/08/2023
  350
  20164.11
  Tricia Plouf
  care of Trupanion Inc
  6100 4th Avenue Suite 400
  Seattle
  WA
  98108
  Common
  03/21/2023
  350
  20878.51
  04/12/2023
  11/29/2022
  /s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Tricia Plouf