content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
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      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001983944</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001761612</issuerCik>
        <issuerName>BICYCLE THERAPEUTICS PLC</issuerName>
        <secFileNumber>001-38916</secFileNumber>
        <issuerAddress>
          <com:street1>BLOCKS A &amp; B, PORTWAY BUILDING</com:street1>
          <com:street2>GRANTA PARK, GREAT ABINGTON</com:street2>
          <com:city>CAMBRIDGE</com:city>
          <com:stateOrCountry>X0</com:stateOrCountry>
          <com:zipCode>CB21 6GS</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>011441223261503</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Thompson Travis Alvin</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Officer</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>Fidelity Brokerage Services LLC</name>
          <address>
            <com:street1>900 Salem Street</com:street1>
            <com:city>Smithfield</com:city>
            <com:stateOrCountry>RI</com:stateOrCountry>
            <com:zipCode>02917</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>6256</noOfUnitsSold>
        <aggregateMarketValue>157112.08</aggregateMarketValue>
        <noOfUnitsOutstanding>47553922</noOfUnitsOutstanding>
        <approxSaleDate>11/11/2024</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>01/03/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>754</amountOfSecuritiesAcquired>
        <paymentDate>01/03/2023</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>04/03/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>226</amountOfSecuritiesAcquired>
        <paymentDate>04/03/2023</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>07/03/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>220</amountOfSecuritiesAcquired>
        <paymentDate>07/03/2023</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>10/03/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>227</amountOfSecuritiesAcquired>
        <paymentDate>10/03/2023</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>01/03/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>928</amountOfSecuritiesAcquired>
        <paymentDate>01/03/2024</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>04/03/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>440</amountOfSecuritiesAcquired>
        <paymentDate>04/03/2024</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>07/03/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>446</amountOfSecuritiesAcquired>
        <paymentDate>07/03/2024</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>10/03/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>440</amountOfSecuritiesAcquired>
        <paymentDate>10/03/2024</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>11/11/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Options Granted - 01/02/2020</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>1109</amountOfSecuritiesAcquired>
        <paymentDate>11/11/2024</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>11/11/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Options Granted - 09/18/2018</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>1312</amountOfSecuritiesAcquired>
        <paymentDate>11/11/2024</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>11/11/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Options Granted- 04/25/2019</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>154</amountOfSecuritiesAcquired>
        <paymentDate>11/11/2024</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>11/11/2024</noticeDate>
        <planAdoptionDates>
          <planAdoptionDate>08/13/2024</planAdoptionDate>
        </planAdoptionDates>
        <signature>/s/ Margaret Campbell, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Travis Thompson</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 8400}]
text: 144
  0001983944
  XXXXXXXX
  LIVE
  0001761612
  BICYCLE THERAPEUTICS PLC
  001-38916
  BLOCKS A & B, PORTWAY BUILDING
  GRANTA PARK, GREAT ABINGTON
  CAMBRIDGE
  X0
  CB21 6GS
  011441223261503
  Thompson Travis Alvin
  Officer
  Common
  Fidelity Brokerage Services LLC
  900 Salem Street
  Smithfield
  RI
  02917
  6256
  157112.08
  47553922
  11/11/2024
  NASDAQ
  Common
  01/03/2023
  Restricted Stock Vesting
  Issuer
  N
  754
  01/03/2023
  Compensation
  Common
  04/03/2023
  Restricted Stock Vesting
  Issuer
  N
  226
  04/03/2023
  Compensation
  Common
  07/03/2023
  Restricted Stock Vesting
  Issuer
  N
  220
  07/03/2023
  Compensation
  Common
  10/03/2023
  Restricted Stock Vesting
  Issuer
  N
  227
  10/03/2023
  Compensation
  Common
  01/03/2024
  Restricted Stock Vesting
  Issuer
  N
  928
  01/03/2024
  Compensation
  Common
  04/03/2024
  Restricted Stock Vesting
  Issuer
  N
  440
  04/03/2024
  Compensation
  Common
  07/03/2024
  Restricted Stock Vesting
  Issuer
  N
  446
  07/03/2024
  Compensation
  Common
  10/03/2024
  Restricted Stock Vesting
  Issuer
  N
  440
  10/03/2024
  Compensation
  Common
  11/11/2024
  Options Granted - 01/02/2020
  Issuer
  N
  1109
  11/11/2024
  Cash
  Common
  11/11/2024
  Options Granted - 09/18/2018
  Issuer
  N
  1312
  11/11/2024
  Cash
  Common
  11/11/2024
  Options Granted- 04/25/2019
  Issuer
  N
  154
  11/11/2024
  Cash
  Y
  11/11/2024
  08/13/2024
  /s/ Margaret Campbell, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Travis Thompson