content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <!-- Generated by Workiva -->
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001180765</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001612042</issuerCik>
        <issuerName>Ascendis Pharma A/S</issuerName>
        <secFileNumber>001-36815</secFileNumber>
        <issuerAddress>
          <com:street1>Tuborg Boulevard 12</com:street1>
          <com:city>Hellerup</com:city>
          <com:stateOrCountry>G7</com:stateOrCountry>
          <com:zipCode>DK - 2900</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>45-70-22-22-44</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Jan Moller Mikkelsen</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>CEO</relationshipToIssuer>
          <relationshipToIssuer>Officer</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>American Depository Shares</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>J.P. Morgan Securities LLC</name>
          <address>
            <com:street1>390 Madison Avenue</com:street1>
            <com:street2>6th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10017</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>24000</noOfUnitsSold>
        <aggregateMarketValue>2348880</aggregateMarketValue>
        <noOfUnitsOutstanding>57152295</noOfUnitsOutstanding>
        <approxSaleDate>11/30/2023</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>American Depository Shares</securitiesClassTitle>
        <acquiredDate>09/11/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Warrant Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>7200</amountOfSecuritiesAcquired>
        <paymentDate>09/11/2023</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>American Depository Shares</securitiesClassTitle>
        <acquiredDate>09/15/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Warrant Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>7200</amountOfSecuritiesAcquired>
        <paymentDate>09/15/2023</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>American Depository Shares</securitiesClassTitle>
        <acquiredDate>09/18/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Warrant Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>5600</amountOfSecuritiesAcquired>
        <paymentDate>09/18/2023</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>American Depository Shares</securitiesClassTitle>
        <acquiredDate>09/25/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Warrant Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>1986</amountOfSecuritiesAcquired>
        <paymentDate>09/25/2023</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>American Depository Shares</securitiesClassTitle>
        <acquiredDate>09/26/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Warrant Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>2014</amountOfSecuritiesAcquired>
        <paymentDate>09/26/2023</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Jan Moller Mikkelsen</name>
          <address>
            <com:street1>C/o Ascendis Pharma A/S</com:street1>
            <com:street2>Tuborg Boulevard 12</com:street2>
            <com:city>Hellerup</com:city>
            <com:stateOrCountry>G7</com:stateOrCountry>
            <com:zipCode>DK-2900</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>American Depository Shares</securitiesClassTitle>
        <saleDate>09/18/2023</saleDate>
        <amountOfSecuritiesSold>8800</amountOfSecuritiesSold>
        <grossProceeds>897065</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Jan Moller Mikkelsen</name>
          <address>
            <com:street1>C/o Ascendis Pharma A/S</com:street1>
            <com:street2>Tuborg Boulevard 12</com:street2>
            <com:city>Hellerup</com:city>
            <com:stateOrCountry>G7</com:stateOrCountry>
            <com:zipCode>DK-2900</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>American Depository Shares</securitiesClassTitle>
        <saleDate>09/22/2023</saleDate>
        <amountOfSecuritiesSold>10000</amountOfSecuritiesSold>
        <grossProceeds>960716</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Jan Moller Mikkelsen</name>
          <address>
            <com:street1>C/o Ascendis Pharma A/S</com:street1>
            <com:street2>Tuborg Boulevard 12</com:street2>
            <com:city>Hellerup</com:city>
            <com:stateOrCountry>G7</com:stateOrCountry>
            <com:zipCode>DK-2900</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>American Depository Shares</securitiesClassTitle>
        <saleDate>09/25/2023</saleDate>
        <amountOfSecuritiesSold>10000</amountOfSecuritiesSold>
        <grossProceeds>954780</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Jan Moller Mikkelsen</name>
          <address>
            <com:street1>C/o Ascendis Pharma A/S</com:street1>
            <com:street2>Tuborg Boulevard 12</com:street2>
            <com:city>Hellerup</com:city>
            <com:stateOrCountry>G7</com:stateOrCountry>
            <com:zipCode>DK-2900</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>American Depository Shares</securitiesClassTitle>
        <saleDate>09/26/2023</saleDate>
        <amountOfSecuritiesSold>20005</amountOfSecuritiesSold>
        <grossProceeds>1896948</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Jan Moller Mikkelsen</name>
          <address>
            <com:street1>C/o Ascendis Pharma A/S</com:street1>
            <com:street2>Tuborg Boulevard 12</com:street2>
            <com:city>Hellerup</com:city>
            <com:stateOrCountry>G7</com:stateOrCountry>
            <com:zipCode>DK-2900</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>American Depository Shares</securitiesClassTitle>
        <saleDate>09/27/2023</saleDate>
        <amountOfSecuritiesSold>4000</amountOfSecuritiesSold>
        <grossProceeds>375757</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Jan Moller Mikkelsen</name>
          <address>
            <com:street1>C/o Ascendis Pharma A/S</com:street1>
            <com:street2>Tuborg Boulevard 12</com:street2>
            <com:city>Hellerup</com:city>
            <com:stateOrCountry>G7</com:stateOrCountry>
            <com:zipCode>DK-2900</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>American Depository Shares</securitiesClassTitle>
        <saleDate>09/28/2023</saleDate>
        <amountOfSecuritiesSold>6809</amountOfSecuritiesSold>
        <grossProceeds>643062</grossProceeds>
      </securitiesSoldInPast3Months>
      <remarks>The shares filed today will be sold to cover taxes.</remarks>
      <noticeSignature>
        <noticeDate>11/30/2023</noticeDate>
        <signature>/s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Jan Moller Mikkelsen</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 9220}]
text: 144
  0001180765
  XXXXXXXX
  LIVE
  0001612042
  Ascendis Pharma A/S
  001-36815
  Tuborg Boulevard 12
  Hellerup
  G7
  DK - 2900
  45-70-22-22-44
  Jan Moller Mikkelsen
  CEO
  Officer
  American Depository Shares
  J.P. Morgan Securities LLC
  390 Madison Avenue
  6th Floor
  New York
  NY
  10017
  24000
  2348880
  57152295
  11/30/2023
  NASDAQ
  American Depository Shares
  09/11/2023
  Warrant Exercise
  Issuer
  N
  7200
  09/11/2023
  Cash
  American Depository Shares
  09/15/2023
  Warrant Exercise
  Issuer
  N
  7200
  09/15/2023
  Cash
  American Depository Shares
  09/18/2023
  Warrant Exercise
  Issuer
  N
  5600
  09/18/2023
  Cash
  American Depository Shares
  09/25/2023
  Warrant Exercise
  Issuer
  N
  1986
  09/25/2023
  Cash
  American Depository Shares
  09/26/2023
  Warrant Exercise
  Issuer
  N
  2014
  09/26/2023
  Cash
  N
  Jan Moller Mikkelsen
  C/o Ascendis Pharma A/S
  Tuborg Boulevard 12
  Hellerup
  G7
  DK-2900
  American Depository Shares
  09/18/2023
  8800
  897065
  Jan Moller Mikkelsen
  C/o Ascendis Pharma A/S
  Tuborg Boulevard 12
  Hellerup
  G7
  DK-2900
  American Depository Shares
  09/22/2023
  10000
  960716
  Jan Moller Mikkelsen
  C/o Ascendis Pharma A/S
  Tuborg Boulevard 12
  Hellerup
  G7
  DK-2900
  American Depository Shares
  09/25/2023
  10000
  954780
  Jan Moller Mikkelsen
  C/o Ascendis Pharma A/S
  Tuborg Boulevard 12
  Hellerup
  G7
  DK-2900
  American Depository Shares
  09/26/2023
  20005
  1896948
  Jan Moller Mikkelsen
  C/o Ascendis Pharma A/S
  Tuborg Boulevard 12
  Hellerup
  G7
  DK-2900
  American Depository Shares
  09/27/2023
  4000
  375757
  Jan Moller Mikkelsen
  C/o Ascendis Pharma A/S
  Tuborg Boulevard 12
  Hellerup
  G7
  DK-2900
  American Depository Shares
  09/28/2023
  6809
  643062
  The shares filed today will be sold to cover taxes.
  11/30/2023
  /s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Jan Moller Mikkelsen