content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
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      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001853904</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001380106</issuerCik>
        <issuerName>RAPID MICRO BIOSYSTEMS, INC.</issuerName>
        <secFileNumber>001-40592</secFileNumber>
        <issuerAddress>
          <com:street1>25 HARTWELL AVENUE</com:street1>
          <com:city>LEXINGTON</com:city>
          <com:stateOrCountry>MA</com:stateOrCountry>
          <com:zipCode>02421</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>978-349-3200</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Spignesi Robert G. Jr.</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Officer</relationshipToIssuer>
          <relationshipToIssuer>Director</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Class A</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>Fidelity Brokerage Services LLC</name>
          <address>
            <com:street1>900 Salem Street</com:street1>
            <com:city>Smithfield</com:city>
            <com:stateOrCountry>RI</com:stateOrCountry>
            <com:zipCode>02917</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>11874</noOfUnitsSold>
        <aggregateMarketValue>39498.86</aggregateMarketValue>
        <noOfUnitsOutstanding>39332172</noOfUnitsOutstanding>
        <approxSaleDate>07/11/2025</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Class A</securitiesClassTitle>
        <acquiredDate>07/10/2025</acquiredDate>
        <natureOfAcquisitionTransaction>Restricted Stock Vesting</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>11874</amountOfSecuritiesAcquired>
        <paymentDate>07/10/2025</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Robert G. Spignesi</name>
          <address>
            <com:street1>25 Hartwell Avenue</com:street1>
            <com:city>Lexington</com:city>
            <com:stateOrCountry>MA</com:stateOrCountry>
            <com:zipCode>02421</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Class A</securitiesClassTitle>
        <saleDate>07/08/2025</saleDate>
        <amountOfSecuritiesSold>10824</amountOfSecuritiesSold>
        <grossProceeds>38021.46</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Robert G. Spignesi</name>
          <address>
            <com:street1>25 Hartwell Avenue</com:street1>
            <com:city>Lexington</com:city>
            <com:stateOrCountry>MA</com:stateOrCountry>
            <com:zipCode>02421</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Class A</securitiesClassTitle>
        <saleDate>07/09/2025</saleDate>
        <amountOfSecuritiesSold>11247</amountOfSecuritiesSold>
        <grossProceeds>39480.34</grossProceeds>
      </securitiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>Robert G. Spignesi</name>
          <address>
            <com:street1>25 Hartwell Avenue</com:street1>
            <com:city>Lexington</com:city>
            <com:stateOrCountry>MA</com:stateOrCountry>
            <com:zipCode>02421</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>Class A</securitiesClassTitle>
        <saleDate>07/10/2025</saleDate>
        <amountOfSecuritiesSold>11617</amountOfSecuritiesSold>
        <grossProceeds>39479.21</grossProceeds>
      </securitiesSoldInPast3Months>
      <remarks>Sale includes an amount necessary to cover a tax obligation resulting from the settlement of a vested equity award distribution.</remarks>
      <noticeSignature>
        <noticeDate>07/11/2025</noticeDate>
        <signature>/s/Wade Moss, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Robert G. Spignesi</signature>
      </noticeSignature>
    </formData>
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text: 144
  0001853904
  XXXXXXXX
  LIVE
  0001380106
  RAPID MICRO BIOSYSTEMS, INC.
  001-40592
  25 HARTWELL AVENUE
  LEXINGTON
  MA
  02421
  978-349-3200
  Spignesi Robert G. Jr.
  Officer
  Director
  Class A
  Fidelity Brokerage Services LLC
  900 Salem Street
  Smithfield
  RI
  02917
  11874
  39498.86
  39332172
  07/11/2025
  NASDAQ
  Class A
  07/10/2025
  Restricted Stock Vesting
  Issuer
  N
  11874
  07/10/2025
  Compensation
  N
  Robert G. Spignesi
  25 Hartwell Avenue
  Lexington
  MA
  02421
  Class A
  07/08/2025
  10824
  38021.46
  Robert G. Spignesi
  25 Hartwell Avenue
  Lexington
  MA
  02421
  Class A
  07/09/2025
  11247
  39480.34
  Robert G. Spignesi
  25 Hartwell Avenue
  Lexington
  MA
  02421
  Class A
  07/10/2025
  11617
  39479.21
  Sale includes an amount necessary to cover a tax obligation resulting from the settlement of a vested equity award distribution.
  07/11/2025
  /s/Wade Moss, as a duly authorized representative of Fidelity Brokerage Services LLC, as attorney-in-fact for Robert G. Spignesi