content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001231933</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001371285</issuerCik>
        <issuerName>TRUPANION, INC.</issuerName>
        <secFileNumber>001-36537</secFileNumber>
        <issuerAddress>
          <com:street1>6100 4TH AVENUE SOUTH</com:street1>
          <com:street2>SUITE 400</com:street2>
          <com:city>SEATTLE</com:city>
          <com:stateOrCountry>WA</com:stateOrCountry>
          <com:zipCode>98108</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>888-733-2685</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>LOW MURRAY B</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Director</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>COMMON</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>RBC CAPITAL MARKETS LLC</name>
          <address>
            <com:street1>250 NICOLLET MALL, SUITE 1700</com:street1>
            <com:city>MINNEAPOLIS</com:city>
            <com:stateOrCountry>MN</com:stateOrCountry>
            <com:zipCode>55401</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>2000</noOfUnitsSold>
        <aggregateMarketValue>105346.00</aggregateMarketValue>
        <noOfUnitsOutstanding>42343514</noOfUnitsOutstanding>
        <approxSaleDate>11/06/2024</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>COMMON</securitiesClassTitle>
        <acquiredDate>12/31/2021</acquiredDate>
        <natureOfAcquisitionTransaction>RSU VEST</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>ISSUER</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>391</amountOfSecuritiesAcquired>
        <paymentDate>12/31/2021</paymentDate>
        <natureOfPayment>N/A</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>COMMON</securitiesClassTitle>
        <acquiredDate>06/30/2021</acquiredDate>
        <natureOfAcquisitionTransaction>RSU VEST</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>ISSUER</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>58</amountOfSecuritiesAcquired>
        <paymentDate>06/30/2021</paymentDate>
        <natureOfPayment>N/A</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>COMMON</securitiesClassTitle>
        <acquiredDate>03/31/2022</acquiredDate>
        <natureOfAcquisitionTransaction>RSU VEST</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>ISSUER</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>378</amountOfSecuritiesAcquired>
        <paymentDate>03/31/2022</paymentDate>
        <natureOfPayment>N/A</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>COMMON</securitiesClassTitle>
        <acquiredDate>06/30/2021</acquiredDate>
        <natureOfAcquisitionTransaction>RSU VEST</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>ISSUER</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>642</amountOfSecuritiesAcquired>
        <paymentDate>06/30/2021</paymentDate>
        <natureOfPayment>N/A</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>COMMON</securitiesClassTitle>
        <acquiredDate>06/30/2022</acquiredDate>
        <natureOfAcquisitionTransaction>RSU VEST</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>ISSUER</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>379</amountOfSecuritiesAcquired>
        <paymentDate>06/30/2022</paymentDate>
        <natureOfPayment>N/A</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>COMMON</securitiesClassTitle>
        <acquiredDate>09/30/2022</acquiredDate>
        <natureOfAcquisitionTransaction>RSU VEST</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>ISSUER</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>152</amountOfSecuritiesAcquired>
        <paymentDate>09/30/2022</paymentDate>
        <natureOfPayment>N/A</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>11/06/2024</noticeDate>
        <signature>POA / JEFFREY EISCHENS</signature>
      </noticeSignature>
    </formData>
  
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 5305}]
text: 144
  0001231933
  XXXXXXXX
  LIVE
  0001371285
  TRUPANION, INC.
  001-36537
  6100 4TH AVENUE SOUTH
  SUITE 400
  SEATTLE
  WA
  98108
  888-733-2685
  LOW MURRAY B
  Director
  COMMON
  RBC CAPITAL MARKETS LLC
  250 NICOLLET MALL, SUITE 1700
  MINNEAPOLIS
  MN
  55401
  2000
  105346.00
  42343514
  11/06/2024
  NASDAQ
  COMMON
  12/31/2021
  RSU VEST
  ISSUER
  N
  391
  12/31/2021
  N/A
  COMMON
  06/30/2021
  RSU VEST
  ISSUER
  N
  58
  06/30/2021
  N/A
  COMMON
  03/31/2022
  RSU VEST
  ISSUER
  N
  378
  03/31/2022
  N/A
  COMMON
  06/30/2021
  RSU VEST
  ISSUER
  N
  642
  06/30/2021
  N/A
  COMMON
  06/30/2022
  RSU VEST
  ISSUER
  N
  379
  06/30/2022
  N/A
  COMMON
  09/30/2022
  RSU VEST
  ISSUER
  N
  152
  09/30/2022
  N/A
  Y
  11/06/2024
  POA / JEFFREY EISCHENS