content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:xsd="http://www.w3.org/2001/XMLSchema" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <headerData>
  <submissionType>144</submissionType>
  <filerInfo>
  <filer>
  <filerCredentials>
  <cik>0001850065</cik>
  <ccc>XXXXXXXX</ccc>
  </filerCredentials>
  </filer>
  <liveTestFlag>LIVE</liveTestFlag>
  </filerInfo>
  </headerData>
  <formData>
  <issuerInfo>
  <issuerCik>0001463361</issuerCik>
  <issuerName>UNITY BIOTECHNOLOGY, INC.</issuerName>
  <secFileNumber>001-38470</secFileNumber>
  <issuerAddress>
  <street1 xmlns="http://www.sec.gov/edgar/common">285 East Grand Ave.</street1>
  <city xmlns="http://www.sec.gov/edgar/common">South San Francisco</city>
  <stateOrCountry xmlns="http://www.sec.gov/edgar/common">CA</stateOrCountry>
  <zipCode xmlns="http://www.sec.gov/edgar/common">94080</zipCode>
  </issuerAddress>
  <issuerContactPhone>(650)-416-1192</issuerContactPhone>
  <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Nguyen Alexander Hieu</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
  <relationshipsToIssuer>
  <relationshipToIssuer>Officer</relationshipToIssuer>
  </relationshipsToIssuer>
  </issuerInfo>
  <securitiesInformation>
  <securitiesClassTitle>Common Stock</securitiesClassTitle>
  <brokerOrMarketmakerDetails>
  <name>Muriel Siebert &amp; Co., LLC</name>
  <address>
  <street1 xmlns="http://www.sec.gov/edgar/common">18045 Oak Street</street1>
  <city xmlns="http://www.sec.gov/edgar/common">Omaha</city>
  <stateOrCountry xmlns="http://www.sec.gov/edgar/common">NE</stateOrCountry>
  <zipCode xmlns="http://www.sec.gov/edgar/common">68130</zipCode>
  </address>
  </brokerOrMarketmakerDetails>
  <noOfUnitsSold>17852</noOfUnitsSold>
  <aggregateMarketValue>18305.44</aggregateMarketValue>
  <noOfUnitsOutstanding>48753</noOfUnitsOutstanding>
  <approxSaleDate>04/02/2025</approxSaleDate>
  <securitiesExchangeName>NASDAQ</securitiesExchangeName>
  </securitiesInformation>
  <securitiesToBeSold>
  <securitiesClassTitle>Common Stock</securitiesClassTitle>
  <acquiredDate>03/28/2025</acquiredDate>
  <natureOfAcquisitionTransaction>Restricted Stock Units granted in 2025 under the Issuer's 2018 Incentive Award Plan</natureOfAcquisitionTransaction>
  <nameOfPersonfromWhomAcquired>UNITY Biotechnology, Inc.</nameOfPersonfromWhomAcquired>
  <isGiftTransaction>N</isGiftTransaction>
  <amountOfSecuritiesAcquired>17852</amountOfSecuritiesAcquired>
  <paymentDate>03/28/2025</paymentDate>
  <natureOfPayment>N/A</natureOfPayment>
  </securitiesToBeSold>
  <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
  <securitiesSoldInPast3Months>
  <sellerDetails>
  <name>Alexander Hieu Nguyen</name>
  <address>
  <street1 xmlns="http://www.sec.gov/edgar/common">C/O UNITY BIOTECHNOLOGY, INC.</street1>
  <street2 xmlns="http://www.sec.gov/edgar/common">285 East Grand Avenue</street2>
  <city xmlns="http://www.sec.gov/edgar/common">South San Francisco</city>
  <stateOrCountry xmlns="http://www.sec.gov/edgar/common">CA</stateOrCountry>
  <zipCode xmlns="http://www.sec.gov/edgar/common">94080</zipCode>
  </address>
  </sellerDetails>
  <securitiesClassTitle>Restricted Stock Units granted in 2024 under the Issuer's 2018 Incentive Award Plan</securitiesClassTitle>
  <saleDate>03/27/2025</saleDate>
  <amountOfSecuritiesSold>1954</amountOfSecuritiesSold>
  <grossProceeds>2267.72</grossProceeds>
  </securitiesSoldInPast3Months>
  <securitiesSoldInPast3Months>
  <sellerDetails>
  <name>Alexander Hieu Nguyen</name>
  <address>
  <street1 xmlns="http://www.sec.gov/edgar/common">C/O UNITY BIOTECHNOLOGY, INC.</street1>
  <street2 xmlns="http://www.sec.gov/edgar/common">285 East Grand Avenue</street2>
  <city xmlns="http://www.sec.gov/edgar/common">South San Francisco</city>
  <stateOrCountry xmlns="http://www.sec.gov/edgar/common">CA</stateOrCountry>
  <zipCode xmlns="http://www.sec.gov/edgar/common">94080</zipCode>
  </address>
  </sellerDetails>
  <securitiesClassTitle>Restricted Stock Units granted in 2022 under the Issuer's 2018 Incentive Award Plan</securitiesClassTitle>
  <saleDate>02/04/2025</saleDate>
  <amountOfSecuritiesSold>164</amountOfSecuritiesSold>
  <grossProceeds>365.72</grossProceeds>
  </securitiesSoldInPast3Months>
  <remarks>1. Share number reflects post-split units after 1-for-10 reverse stock split as of 10/19/22.</remarks>
  <noticeSignature>
  <noticeDate>04/03/2025</noticeDate>
  <signature>/s/ Alexander Hieu Nguyen</signature>
  </noticeSignature>
  </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 4443}]
text: 144
  0001850065
  XXXXXXXX
  LIVE
  0001463361
  UNITY BIOTECHNOLOGY, INC.
  001-38470
  285 East Grand Ave.
  South San Francisco
  CA
  94080
  (650)-416-1192
  Nguyen Alexander Hieu
  Officer
  Common Stock
  Muriel Siebert & Co., LLC
  18045 Oak Street
  Omaha
  NE
  68130
  17852
  18305.44
  48753
  04/02/2025
  NASDAQ
  Common Stock
  03/28/2025
  Restricted Stock Units granted in 2025 under the Issuer's 2018 Incentive Award Plan
  UNITY Biotechnology, Inc.
  N
  17852
  03/28/2025
  N/A
  N
  Alexander Hieu Nguyen
  C/O UNITY BIOTECHNOLOGY, INC.
  285 East Grand Avenue
  South San Francisco
  CA
  94080
  Restricted Stock Units granted in 2024 under the Issuer's 2018 Incentive Award Plan
  03/27/2025
  1954
  2267.72
  Alexander Hieu Nguyen
  C/O UNITY BIOTECHNOLOGY, INC.
  285 East Grand Avenue
  South San Francisco
  CA
  94080
  Restricted Stock Units granted in 2022 under the Issuer's 2018 Incentive Award Plan
  02/04/2025
  164
  365.72
  1. Share number reflects post-split units after 1-for-10 reverse stock split as of 10/19/22.
  04/03/2025
  /s/ Alexander Hieu Nguyen