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  <issuerName>UNITY BIOTECHNOLOGY, INC.</issuerName>
  <secFileNumber>001-38470</secFileNumber>
  <issuerAddress>
  <street1 xmlns="http://www.sec.gov/edgar/common">285 East Grand Ave.</street1>
  <city xmlns="http://www.sec.gov/edgar/common">South San Francisco</city>
  <stateOrCountry xmlns="http://www.sec.gov/edgar/common">CA</stateOrCountry>
  <zipCode xmlns="http://www.sec.gov/edgar/common">94080</zipCode>
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  <issuerContactPhone>(650)-416-1192</issuerContactPhone>
  <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Ghosh Anirvan</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
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  <relationshipToIssuer>Officer</relationshipToIssuer>
  <relationshipToIssuer>Director</relationshipToIssuer>
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  <brokerOrMarketmakerDetails>
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  <street1 xmlns="http://www.sec.gov/edgar/common">Harborside 2, 200 Hudson Street</street1>
  <street2 xmlns="http://www.sec.gov/edgar/common">Ste. 501</street2>
  <city xmlns="http://www.sec.gov/edgar/common">Jersey City</city>
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  <zipCode xmlns="http://www.sec.gov/edgar/common">07311</zipCode>
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  <noOfUnitsOutstanding>82222</noOfUnitsOutstanding>
  <approxSaleDate>09/14/2023</approxSaleDate>
  <securitiesExchangeName>NASDAQ</securitiesExchangeName>
  </securitiesInformation>
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  <securitiesClassTitle>Common Stock</securitiesClassTitle>
  <acquiredDate>09/13/2023</acquiredDate>
  <natureOfAcquisitionTransaction>Restricted Stock Units granted in 2022 under the Issuer's 2018 Incentive Award Plan</natureOfAcquisitionTransaction>
  <nameOfPersonfromWhomAcquired>UNITY Biotechnology, Inc.</nameOfPersonfromWhomAcquired>
  <isGiftTransaction>N</isGiftTransaction>
  <amountOfSecuritiesAcquired>1246</amountOfSecuritiesAcquired>
  <paymentDate>09/13/2023</paymentDate>
  <natureOfPayment>N/A</natureOfPayment>
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  <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
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  <name>Anirvan Ghosh c/o UNITY Biotechnology, Inc.</name>
  <address>
  <street1 xmlns="http://www.sec.gov/edgar/common">285 East Grand Ave.</street1>
  <city xmlns="http://www.sec.gov/edgar/common">South San Francisco</city>
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  <zipCode xmlns="http://www.sec.gov/edgar/common">94080</zipCode>
  </address>
  </sellerDetails>
  <securitiesClassTitle>Restricted Stock Units granted in 2020 under the Issuer's 2018 Incentive Award Plan</securitiesClassTitle>
  <saleDate>06/14/2023</saleDate>
  <amountOfSecuritiesSold>1212</amountOfSecuritiesSold>
  <grossProceeds>4108.68</grossProceeds>
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  <securitiesClassTitle>Restricted Stock Units granted in 2021 under the Issuer's 2018 Incentive Award Plan</securitiesClassTitle>
  <saleDate>06/26/2023</saleDate>
  <amountOfSecuritiesSold>1177</amountOfSecuritiesSold>
  <grossProceeds>3766.64</grossProceeds>
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  <street1 xmlns="http://www.sec.gov/edgar/common">285 East Grand Ave.</street1>
  <city xmlns="http://www.sec.gov/edgar/common">South San Francisco</city>
  <stateOrCountry xmlns="http://www.sec.gov/edgar/common">CA</stateOrCountry>
  <zipCode xmlns="http://www.sec.gov/edgar/common">94080</zipCode>
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  <securitiesClassTitle>Restricted Stock Units granted in 2021 under the Issuer's 2018 Incentive Award Plan</securitiesClassTitle>
  <saleDate>07/31/2023</saleDate>
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  <grossProceeds>2917.02</grossProceeds>
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  <name>Anirvan Ghosh c/o UNITY Biotechnology, Inc.</name>
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  <securitiesClassTitle>Restricted Stock Units granted in 2022 under the Issuer's 2018 Incentive Award Plan</securitiesClassTitle>
  <saleDate>08/02/2023</saleDate>
  <amountOfSecuritiesSold>581</amountOfSecuritiesSold>
  <grossProceeds>1708.09</grossProceeds>
  </securitiesSoldInPast3Months>
  <remarks>1. Share number reflects post-split units after 1-for-10 reverse stock split as of 10/19/22.</remarks>
  <noticeSignature>
  <noticeDate>09/15/2023</noticeDate>
  <signature>/s/ Alexander Nguyen as attorney-in-fact for Anirvan Ghosh pursuant to Power of Attorney</signature>
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text: 144
  0001807437
  XXXXXXXX
  LIVE
  0001463361
  UNITY BIOTECHNOLOGY, INC.
  001-38470
  285 East Grand Ave.
  South San Francisco
  CA
  94080
  (650)-416-1192
  Ghosh Anirvan
  Officer
  Director
  Common Stock
  E*TRADE Securities LLC
  Harborside 2, 200 Hudson Street
  Ste. 501
  Jersey City
  NJ
  07311
  1246
  3078.70
  82222
  09/14/2023
  NASDAQ
  Common Stock
  09/13/2023
  Restricted Stock Units granted in 2022 under the Issuer's 2018 Incentive Award Plan
  UNITY Biotechnology, Inc.
  N
  1246
  09/13/2023
  N/A
  N
  Anirvan Ghosh c/o UNITY Biotechnology, Inc.
  285 East Grand Ave.
  South San Francisco
  CA
  94080
  Restricted Stock Units granted in 2020 under the Issuer's 2018 Incentive Award Plan
  06/14/2023
  1212
  4108.68
  Anirvan Ghosh c/o UNITY Biotechnology, Inc.
  285 East Grand Ave.
  South San Francisco
  CA
  94080
  Restricted Stock Units granted in 2021 under the Issuer's 2018 Incentive Award Plan
  06/26/2023
  1177
  3766.64
  Anirvan Ghosh c/o UNITY Biotechnology, Inc.
  285 East Grand Ave.
  South San Francisco
  CA
  94080
  Restricted Stock Units granted in 2021 under the Issuer's 2018 Incentive Award Plan
  07/31/2023
  1020
  2917.02
  Anirvan Ghosh c/o UNITY Biotechnology, Inc.
  285 East Grand Ave.
  South San Francisco
  CA
  94080
  Restricted Stock Units granted in 2022 under the Issuer's 2018 Incentive Award Plan
  08/02/2023
  581
  1708.09
  1. Share number reflects post-split units after 1-for-10 reverse stock split as of 10/19/22.
  09/15/2023
  /s/ Alexander Nguyen as attorney-in-fact for Anirvan Ghosh pursuant to Power of Attorney