content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <!-- Generated by Workiva -->
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001120477</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001377121</issuerCik>
        <issuerName>Protagonist Therapeutics, Inc.</issuerName>
        <secFileNumber>001-37852</secFileNumber>
        <issuerAddress>
          <com:street1>7707 Gateway Boulevard, Suite 140</com:street1>
          <com:city>Newark</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94560</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>5104740170</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Dinesh Patel</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>CEO</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Commom</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>J.P. Morgan Securities LLC</name>
          <address>
            <com:street1>390 Madison Avenue</com:street1>
            <com:street2>6th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10017</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>100000</noOfUnitsSold>
        <aggregateMarketValue>4593000</aggregateMarketValue>
        <noOfUnitsOutstanding>59598018</noOfUnitsOutstanding>
        <approxSaleDate>11/25/2024</approxSaleDate>
        <securitiesExchangeName>Nasdaq</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>05/09/2017</acquiredDate>
        <natureOfAcquisitionTransaction>Option Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>100000</amountOfSecuritiesAcquired>
        <paymentDate>05/09/2017</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <remarks>This filing is for shares acquired by Dinesh  in the form of Executive Stock Compensation from  05/09/2017 - 05/04/2022.</remarks>
      <noticeSignature>
        <noticeDate>11/25/2024</noticeDate>
        <planAdoptionDates>
          <planAdoptionDate>03/20/2023</planAdoptionDate>
        </planAdoptionDates>
        <signature>/s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Dinesh Patel.</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 3115}]
text: 144
  0001120477
  XXXXXXXX
  LIVE
  0001377121
  Protagonist Therapeutics, Inc.
  001-37852
  7707 Gateway Boulevard, Suite 140
  Newark
  CA
  94560
  5104740170
  Dinesh Patel
  CEO
  Commom
  J.P. Morgan Securities LLC
  390 Madison Avenue
  6th Floor
  New York
  NY
  10017
  100000
  4593000
  59598018
  11/25/2024
  Nasdaq
  Common
  05/09/2017
  Option Exercise
  Issuer
  N
  100000
  05/09/2017
  Compensation
  Y
  This filing is for shares acquired by Dinesh  in the form of Executive Stock Compensation from  05/09/2017 - 05/04/2022.
  11/25/2024
  03/20/2023
  /s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Dinesh Patel.