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  <street2 xmlns="http://www.sec.gov/edgar/common">Fourth Floor</street2>
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  <securitiesExchangeName>Nasdaq</securitiesExchangeName>
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  <acquiredDate>05/22/2023</acquiredDate>
  <natureOfAcquisitionTransaction>Restricted Stock Unit Release</natureOfAcquisitionTransaction>
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  <zipCode xmlns="http://www.sec.gov/edgar/common">94080</zipCode>
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  <saleDate>03/30/2023</saleDate>
  <amountOfSecuritiesSold>14883</amountOfSecuritiesSold>
  <grossProceeds>12252</grossProceeds>
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  <noticeSignature>
  <noticeDate>05/23/2023</noticeDate>
  <signature>/s/ John G. McHutchison</signature>
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text: 144
  0001738125
  XXXXXXXX
  LIVE
  0001426800
  Assembly Biosciences, Inc.
  001-35005
  331 Oyster Point Blvd.
  Fourth Floor
  South San Francisco
  CA
  94080
  833-509-4583
  John G. McHutchison
  Director
  Common Stock
  Morgan Stanley Smith Barney LLC
  1 New York Plaza, 38th Floor
  New York
  NY
  10004
  25972
  27019
  52121309
  05/23/2023
  Nasdaq
  Common Stock
  05/22/2023
  Restricted Stock Unit Release
  Assembly Biosciences, Inc.
  N
  25972
  05/23/2023
  N/A
  N
  John McHutchison
  c/o 331 Oyster Point Blvd., Fourth Floor
  South San Francisco
  CA
  94080
  Common Stock
  03/30/2023
  14883
  12252
  05/23/2023
  /s/ John G. McHutchison