content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001231933</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001371285</issuerCik>
        <issuerName>TRUPANION, INC.</issuerName>
        <secFileNumber>001-36537</secFileNumber>
        <issuerAddress>
          <com:street1>6100 4TH AVENUE SOUTH</com:street1>
          <com:street2>SUITE 400</com:street2>
          <com:city>SEATTLE</com:city>
          <com:stateOrCountry>WA</com:stateOrCountry>
          <com:zipCode>98108</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>888-733-2685</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>LOW MURRAY B</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>Director</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>COMMON</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>RBC CAPITAL MARKETS LLC</name>
          <address>
            <com:street1>250 NICOLLET MALL, SUITE 1700</com:street1>
            <com:city>MINNEAPOLIS</com:city>
            <com:stateOrCountry>MN</com:stateOrCountry>
            <com:zipCode>55401</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>192</noOfUnitsSold>
        <aggregateMarketValue>9600.00</aggregateMarketValue>
        <noOfUnitsOutstanding>42343514</noOfUnitsOutstanding>
        <approxSaleDate>01/03/2025</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>COMMON</securitiesClassTitle>
        <acquiredDate>06/15/2006</acquiredDate>
        <natureOfAcquisitionTransaction>RESTRICTED STOCK VEST</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>ISSUER</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>192</amountOfSecuritiesAcquired>
        <paymentDate>06/15/2006</paymentDate>
        <natureOfPayment>N/A</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <securitiesSoldInPast3Months>
        <sellerDetails>
          <name>MURRAY LOW C/O RBC CAPITAL MARKETS LLC</name>
          <address>
            <com:street1>250 NICOLLET MALL, SUITE 1700</com:street1>
            <com:city>MINNEAPOLIS</com:city>
            <com:stateOrCountry>MN</com:stateOrCountry>
            <com:zipCode>55401</com:zipCode>
          </address>
        </sellerDetails>
        <securitiesClassTitle>TRUPANION INC</securitiesClassTitle>
        <saleDate>11/06/2024</saleDate>
        <amountOfSecuritiesSold>2000</amountOfSecuritiesSold>
        <grossProceeds>105346.00</grossProceeds>
      </securitiesSoldInPast3Months>
      <remarks>SOLD PURSUANT TO A RULE 10B5-1 PLAN ESTABLISHED 08/27/2024</remarks>
      <noticeSignature>
        <noticeDate>01/03/2025</noticeDate>
        <planAdoptionDates>
          <planAdoptionDate>08/27/2024</planAdoptionDate>
        </planAdoptionDates>
        <signature>POA / JEFFREY EISCHENS</signature>
      </noticeSignature>
    </formData>
  
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 3575}]
text: 144
  0001231933
  XXXXXXXX
  LIVE
  0001371285
  TRUPANION, INC.
  001-36537
  6100 4TH AVENUE SOUTH
  SUITE 400
  SEATTLE
  WA
  98108
  888-733-2685
  LOW MURRAY B
  Director
  COMMON
  RBC CAPITAL MARKETS LLC
  250 NICOLLET MALL, SUITE 1700
  MINNEAPOLIS
  MN
  55401
  192
  9600.00
  42343514
  01/03/2025
  NASDAQ
  COMMON
  06/15/2006
  RESTRICTED STOCK VEST
  ISSUER
  N
  192
  06/15/2006
  N/A
  N
  MURRAY LOW C/O RBC CAPITAL MARKETS LLC
  250 NICOLLET MALL, SUITE 1700
  MINNEAPOLIS
  MN
  55401
  TRUPANION INC
  11/06/2024
  2000
  105346.00
  SOLD PURSUANT TO A RULE 10B5-1 PLAN ESTABLISHED 08/27/2024
  01/03/2025
  08/27/2024
  POA / JEFFREY EISCHENS