content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:xsd="http://www.w3.org/2001/XMLSchema" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
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  <cik>0001807437</cik>
  <ccc>XXXXXXXX</ccc>
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  <liveTestFlag>LIVE</liveTestFlag>
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  <issuerInfo>
  <issuerCik>0001463361</issuerCik>
  <issuerName>UNITY BIOTECHNOLOGY, INC.</issuerName>
  <secFileNumber>001-38470</secFileNumber>
  <issuerAddress>
  <street1 xmlns="http://www.sec.gov/edgar/common">285 East Grand Ave.</street1>
  <city xmlns="http://www.sec.gov/edgar/common">South San Francisco</city>
  <stateOrCountry xmlns="http://www.sec.gov/edgar/common">CA</stateOrCountry>
  <zipCode xmlns="http://www.sec.gov/edgar/common">94080</zipCode>
  </issuerAddress>
  <issuerContactPhone>(650)-416-1192</issuerContactPhone>
  <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Ghosh Anirvan</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
  <relationshipsToIssuer>
  <relationshipToIssuer>Officer</relationshipToIssuer>
  <relationshipToIssuer>Director</relationshipToIssuer>
  </relationshipsToIssuer>
  </issuerInfo>
  <securitiesInformation>
  <securitiesClassTitle>Common Stock</securitiesClassTitle>
  <brokerOrMarketmakerDetails>
  <name>Muriel Siebert &amp; Co., LLC</name>
  <address>
  <street1 xmlns="http://www.sec.gov/edgar/common">18045 Oak Street</street1>
  <city xmlns="http://www.sec.gov/edgar/common">Omaha</city>
  <stateOrCountry xmlns="http://www.sec.gov/edgar/common">NE</stateOrCountry>
  <zipCode xmlns="http://www.sec.gov/edgar/common">68130</zipCode>
  </address>
  </brokerOrMarketmakerDetails>
  <noOfUnitsSold>96797</noOfUnitsSold>
  <aggregateMarketValue>99255.64</aggregateMarketValue>
  <noOfUnitsOutstanding>153454</noOfUnitsOutstanding>
  <approxSaleDate>04/02/2025</approxSaleDate>
  <securitiesExchangeName>NASDAQ</securitiesExchangeName>
  </securitiesInformation>
  <securitiesToBeSold>
  <securitiesClassTitle>Common Stock</securitiesClassTitle>
  <acquiredDate>03/28/2025</acquiredDate>
  <natureOfAcquisitionTransaction>Restricted Stock Units granted in 2025 under the Issuer's 2018 Incentive Award Plan</natureOfAcquisitionTransaction>
  <nameOfPersonfromWhomAcquired>UNITY Biotechnology, Inc.</nameOfPersonfromWhomAcquired>
  <isGiftTransaction>N</isGiftTransaction>
  <amountOfSecuritiesAcquired>96797</amountOfSecuritiesAcquired>
  <paymentDate>03/28/2025</paymentDate>
  <natureOfPayment>N/A</natureOfPayment>
  </securitiesToBeSold>
  <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
  <securitiesSoldInPast3Months>
  <sellerDetails>
  <name>Anirvan Ghosh</name>
  <address>
  <street1 xmlns="http://www.sec.gov/edgar/common">C/O UNITY BIOTECHNOLOGY, INC.</street1>
  <street2 xmlns="http://www.sec.gov/edgar/common">285 East Grand Avenue</street2>
  <city xmlns="http://www.sec.gov/edgar/common">South San Francisco</city>
  <stateOrCountry xmlns="http://www.sec.gov/edgar/common">CA</stateOrCountry>
  <zipCode xmlns="http://www.sec.gov/edgar/common">94080</zipCode>
  </address>
  </sellerDetails>
  <securitiesClassTitle>Restricted Stock Units granted in 2022 under the Issuer's 2018 Incentive Award Plan</securitiesClassTitle>
  <saleDate>02/04/2025</saleDate>
  <amountOfSecuritiesSold>642</amountOfSecuritiesSold>
  <grossProceeds>1431.66</grossProceeds>
  </securitiesSoldInPast3Months>
  <remarks>1. Share number reflects post-split units after 1-for-10 reverse stock split as of 10/19/22.</remarks>
  <noticeSignature>
  <noticeDate>04/03/2025</noticeDate>
  <signature>/s/ Alexander Nguyen as attorney-in-fact for Anirvan Ghosh pursuant to Power of Attorney</signature>
  </noticeSignature>
  </formData>
  </edgarSubmission>
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text: 144
  0001807437
  XXXXXXXX
  LIVE
  0001463361
  UNITY BIOTECHNOLOGY, INC.
  001-38470
  285 East Grand Ave.
  South San Francisco
  CA
  94080
  (650)-416-1192
  Ghosh Anirvan
  Officer
  Director
  Common Stock
  Muriel Siebert & Co., LLC
  18045 Oak Street
  Omaha
  NE
  68130
  96797
  99255.64
  153454
  04/02/2025
  NASDAQ
  Common Stock
  03/28/2025
  Restricted Stock Units granted in 2025 under the Issuer's 2018 Incentive Award Plan
  UNITY Biotechnology, Inc.
  N
  96797
  03/28/2025
  N/A
  N
  Anirvan Ghosh
  C/O UNITY BIOTECHNOLOGY, INC.
  285 East Grand Avenue
  South San Francisco
  CA
  94080
  Restricted Stock Units granted in 2022 under the Issuer's 2018 Incentive Award Plan
  02/04/2025
  642
  1431.66
  1. Share number reflects post-split units after 1-for-10 reverse stock split as of 10/19/22.
  04/03/2025
  /s/ Alexander Nguyen as attorney-in-fact for Anirvan Ghosh pursuant to Power of Attorney