content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
    <headerData>
      <submissionType>144</submissionType>
      <filerInfo>
        <filer>
          <filerCredentials>
            <cik>0001380585</cik>
            <ccc>XXXXXXXX</ccc>
          </filerCredentials>
        </filer>
        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
      </filerInfo>
    </headerData>
    <formData>
      <issuerInfo>
        <issuerCik>0001642375</issuerCik>
        <issuerName>Guardion Health Sciences, Inc.</issuerName>
        <secFileNumber>001-38861</secFileNumber>
        <issuerAddress>
          <com:street1>2925 RICHMOND AVENUE</com:street1>
          <com:street2>SUITE 1200</com:street2>
          <com:city>HOUSTON</com:city>
          <com:stateOrCountry>TX</com:stateOrCountry>
          <com:zipCode>77098</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>(800) 873-5141</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Radoff Bradley Louis</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>10% Stockholder</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Common Stock, par value $0.001 per share</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>Morgan Stanley</name>
          <address>
            <com:street1>1585 Broadway</com:street1>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10036</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>217900</noOfUnitsSold>
        <aggregateMarketValue>3593171.00</aggregateMarketValue>
        <noOfUnitsOutstanding>1284156</noOfUnitsOutstanding>
        <approxSaleDate>08/26/2024</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
      </securitiesInformation>
      <securitiesInformation>
        <securitiesClassTitle>Common Stock, par value $0.001 per share</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>UBS Financial Services Inc.</name>
          <address>
            <com:street1>1000 Harbor Blvd.</com:street1>
            <com:city>Wehawken</com:city>
            <com:stateOrCountry>NJ</com:stateOrCountry>
            <com:zipCode>07086</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>25100</noOfUnitsSold>
        <aggregateMarketValue>413899.00</aggregateMarketValue>
        <noOfUnitsOutstanding>1284156</noOfUnitsOutstanding>
        <approxSaleDate>08/26/2024</approxSaleDate>
        <securitiesExchangeName>NASDAQ</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common Stock, par value $0.001 per share</securitiesClassTitle>
        <acquiredDate>10/06/2022</acquiredDate>
        <natureOfAcquisitionTransaction>Open Market Purchases</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>N/A</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>217900</amountOfSecuritiesAcquired>
        <paymentDate>10/06/2022</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common Stock, par value $0.001 per share</securitiesClassTitle>
        <acquiredDate>11/25/2022</acquiredDate>
        <natureOfAcquisitionTransaction>Open Market Purchases</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>N/A</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>25100</amountOfSecuritiesAcquired>
        <paymentDate>11/25/2022</paymentDate>
        <natureOfPayment>Cash</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <remarks>The aggregate market value is as of market open on 08/26/2024. The number of securities outstanding is as of 07/31/2024. The 217,900 shares proposed to be sold held directly by Mr. Radoff were acquired and paid for between 10/06/2022 and 01/09/2023. The 25,100 shares proposed to be sold held directly by The Radoff Family Foundation, of which Mr. Radoff serves as director, were acquired and paid for between 11/25/2022 and 12/12/2022.</remarks>
      <noticeSignature>
        <noticeDate>08/26/2024</noticeDate>
        <signature>/s/ Bradley L. Radoff</signature>
      </noticeSignature>
    </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 4624}]
text: 144
  0001380585
  XXXXXXXX
  LIVE
  0001642375
  Guardion Health Sciences, Inc.
  001-38861
  2925 RICHMOND AVENUE
  SUITE 1200
  HOUSTON
  TX
  77098
  (800) 873-5141
  Radoff Bradley Louis
  10% Stockholder
  Common Stock, par value $0.001 per share
  Morgan Stanley
  1585 Broadway
  New York
  NY
  10036
  217900
  3593171.00
  1284156
  08/26/2024
  NASDAQ
  Common Stock, par value $0.001 per share
  UBS Financial Services Inc.
  1000 Harbor Blvd.
  Wehawken
  NJ
  07086
  25100
  413899.00
  1284156
  08/26/2024
  NASDAQ
  Common Stock, par value $0.001 per share
  10/06/2022
  Open Market Purchases
  N/A
  N
  217900
  10/06/2022
  Cash
  Common Stock, par value $0.001 per share
  11/25/2022
  Open Market Purchases
  N/A
  N
  25100
  11/25/2022
  Cash
  Y
  The aggregate market value is as of market open on 08/26/2024. The number of securities outstanding is as of 07/31/2024. The 217,900 shares proposed to be sold held directly by Mr. Radoff were acquired and paid for between 10/06/2022 and 01/09/2023. The 25,100 shares proposed to be sold held directly by The Radoff Family Foundation, of which Mr. Radoff serves as director, were acquired and paid for between 11/25/2022 and 12/12/2022.
  08/26/2024
  /s/ Bradley L. Radoff