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        <filer>
          <filerCredentials>
            <cik>0001120477</cik>
            <ccc>XXXXXXXX</ccc>
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        <liveTestFlag>LIVE</liveTestFlag>
  
  
  
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        <issuerCik>0001377121</issuerCik>
        <issuerName>Protagonist Therapeutics, Inc.</issuerName>
        <secFileNumber>001-37852</secFileNumber>
        <issuerAddress>
          <com:street1>7707 Gateway Boulevard, Suite 140</com:street1>
          <com:city>Newark</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94560</com:zipCode>
        </issuerAddress>
        <issuerContactPhone>5104740170</issuerContactPhone>
        <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Dinesh Patel</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
        <relationshipsToIssuer>
          <relationshipToIssuer>CEO</relationshipToIssuer>
        </relationshipsToIssuer>
      </issuerInfo>
      <securitiesInformation>
        <securitiesClassTitle>Commom</securitiesClassTitle>
        <brokerOrMarketmakerDetails>
          <name>J.P. Morgan Securities LLC</name>
          <address>
            <com:street1>390 Madison Avenue</com:street1>
            <com:street2>6th Floor</com:street2>
            <com:city>New York</com:city>
            <com:stateOrCountry>NY</com:stateOrCountry>
            <com:zipCode>10017</com:zipCode>
          </address>
        </brokerOrMarketmakerDetails>
        <noOfUnitsSold>50000</noOfUnitsSold>
        <aggregateMarketValue>2761000</aggregateMarketValue>
        <noOfUnitsOutstanding>61981955</noOfUnitsOutstanding>
        <approxSaleDate>07/22/2025</approxSaleDate>
        <securitiesExchangeName>Nasdaq</securitiesExchangeName>
      </securitiesInformation>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>02/05/2020</acquiredDate>
        <natureOfAcquisitionTransaction>Option Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>23989</amountOfSecuritiesAcquired>
        <paymentDate>02/05/2020</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>10/18/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Option Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>6000</amountOfSecuritiesAcquired>
        <paymentDate>10/18/2023</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>03/08/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Option Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>825</amountOfSecuritiesAcquired>
        <paymentDate>03/08/2024</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>09/08/2023</acquiredDate>
        <natureOfAcquisitionTransaction>Option Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>401</amountOfSecuritiesAcquired>
        <paymentDate>09/08/2023</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>02/15/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Option Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>5700</amountOfSecuritiesAcquired>
        <paymentDate>02/15/2024</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <securitiesToBeSold>
        <securitiesClassTitle>Common</securitiesClassTitle>
        <acquiredDate>02/26/2024</acquiredDate>
        <natureOfAcquisitionTransaction>Option Exercise</natureOfAcquisitionTransaction>
        <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
        <isGiftTransaction>N</isGiftTransaction>
        <amountOfSecuritiesAcquired>13085</amountOfSecuritiesAcquired>
        <paymentDate>02/26/2024</paymentDate>
        <natureOfPayment>Compensation</natureOfPayment>
      </securitiesToBeSold>
      <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
      <noticeSignature>
        <noticeDate>07/22/2025</noticeDate>
        <signature>/s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Dinesh Patel.</signature>
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text: 144
  0001120477
  XXXXXXXX
  LIVE
  0001377121
  Protagonist Therapeutics, Inc.
  001-37852
  7707 Gateway Boulevard, Suite 140
  Newark
  CA
  94560
  5104740170
  Dinesh Patel
  CEO
  Commom
  J.P. Morgan Securities LLC
  390 Madison Avenue
  6th Floor
  New York
  NY
  10017
  50000
  2761000
  61981955
  07/22/2025
  Nasdaq
  Common
  02/05/2020
  Option Exercise
  Issuer
  N
  23989
  02/05/2020
  Compensation
  Common
  10/18/2023
  Option Exercise
  Issuer
  N
  6000
  10/18/2023
  Compensation
  Common
  03/08/2024
  Option Exercise
  Issuer
  N
  825
  03/08/2024
  Compensation
  Common
  09/08/2023
  Option Exercise
  Issuer
  N
  401
  09/08/2023
  Compensation
  Common
  02/15/2024
  Option Exercise
  Issuer
  N
  5700
  02/15/2024
  Compensation
  Common
  02/26/2024
  Option Exercise
  Issuer
  N
  13085
  02/26/2024
  Compensation
  Y
  07/22/2025
  /s/ J.P. Morgan Securities LLC as agent and attorney-in-fact for Dinesh Patel.