content: <?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:xsd="http://www.w3.org/2001/XMLSchema" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
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  <cik>0001667937</cik>
  <ccc>XXXXXXXX</ccc>
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  <liveTestFlag>LIVE</liveTestFlag>
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  <issuerCik>0001858848</issuerCik>
  <issuerName>Tenaya Therapeutics, Inc.</issuerName>
  <secFileNumber>001-40656</secFileNumber>
  <issuerAddress>
  <street1 xmlns="http://www.sec.gov/edgar/common">171 Oyster Point Boulevard</street1>
  <street2 xmlns="http://www.sec.gov/edgar/common">5th Floor</street2>
  <city xmlns="http://www.sec.gov/edgar/common">South San Francisco</city>
  <stateOrCountry xmlns="http://www.sec.gov/edgar/common">CA</stateOrCountry>
  <zipCode xmlns="http://www.sec.gov/edgar/common">94080</zipCode>
  </issuerAddress>
  <issuerContactPhone>(650)-825-6900</issuerContactPhone>
  <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Faraz Ali</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
  <relationshipsToIssuer>
  <relationshipToIssuer>Officer</relationshipToIssuer>
  <relationshipToIssuer>Director</relationshipToIssuer>
  </relationshipsToIssuer>
  </issuerInfo>
  <securitiesInformation>
  <securitiesClassTitle>Common Stock</securitiesClassTitle>
  <brokerOrMarketmakerDetails>
  <name>Morgan Stanley Smith Barney</name>
  <address>
  <street1 xmlns="http://www.sec.gov/edgar/common">Operations Department</street1>
  <street2 xmlns="http://www.sec.gov/edgar/common">2000 Westchester Ave</street2>
  <city xmlns="http://www.sec.gov/edgar/common">Purchase</city>
  <stateOrCountry xmlns="http://www.sec.gov/edgar/common">NY</stateOrCountry>
  <zipCode xmlns="http://www.sec.gov/edgar/common">10577-2530</zipCode>
  </address>
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  <noOfUnitsSold>6358</noOfUnitsSold>
  <aggregateMarketValue>40182.56</aggregateMarketValue>
  <noOfUnitsOutstanding>76999909</noOfUnitsOutstanding>
  <approxSaleDate>02/16/2024</approxSaleDate>
  <securitiesExchangeName>Nasdaq Global Select</securitiesExchangeName>
  </securitiesInformation>
  <securitiesToBeSold>
  <securitiesClassTitle>Common Stock</securitiesClassTitle>
  <acquiredDate>02/16/2024</acquiredDate>
  <natureOfAcquisitionTransaction>Restricted Stock Unit</natureOfAcquisitionTransaction>
  <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
  <isGiftTransaction>N</isGiftTransaction>
  <amountOfSecuritiesAcquired>15625</amountOfSecuritiesAcquired>
  <paymentDate>02/16/2024</paymentDate>
  <natureOfPayment>N/A</natureOfPayment>
  </securitiesToBeSold>
  <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
  <noticeSignature>
  <noticeDate>02/16/2024</noticeDate>
  <signature>/s/ Faraz Ali</signature>
  </noticeSignature>
  </formData>
  </edgarSubmission>
metadata_documents: [{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 2889}]
text: 144
  0001667937
  XXXXXXXX
  LIVE
  0001858848
  Tenaya Therapeutics, Inc.
  001-40656
  171 Oyster Point Boulevard
  5th Floor
  South San Francisco
  CA
  94080
  (650)-825-6900
  Faraz Ali
  Officer
  Director
  Common Stock
  Morgan Stanley Smith Barney
  Operations Department
  2000 Westchester Ave
  Purchase
  NY
  10577-2530
  6358
  40182.56
  76999909
  02/16/2024
  Nasdaq Global Select
  Common Stock
  02/16/2024
  Restricted Stock Unit
  Issuer
  N
  15625
  02/16/2024
  N/A
  Y
  02/16/2024
  /s/ Faraz Ali