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    <submissionType>144</submissionType>
    <filerInfo>
      <filer>
        <filerCredentials>
          <cik>0001710030</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>



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  <formData>
    <issuerInfo>
      <issuerCik>0001531031</issuerCik>
      <issuerName>Esquire Financial Holdings, Inc.</issuerName>
      <secFileNumber>001-38131</secFileNumber>
      <issuerAddress>
        <com:street1>100 JERICHO QUADRANGLE</com:street1>
        <com:street2>SUITE 100</com:street2>
        <com:city>JERICHO</com:city>
        <com:stateOrCountry>NY</com:stateOrCountry>
        <com:zipCode>11753</com:zipCode>
      </issuerAddress>
      <issuerContactPhone>(800) 996-0213</issuerContactPhone>
      <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Waterhouse Kevin C</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <relationshipsToIssuer>
        <relationshipToIssuer>Director</relationshipToIssuer>
      </relationshipsToIssuer>
    </issuerInfo>
    <securitiesInformation>
      <securitiesClassTitle>Common Stock</securitiesClassTitle>
      <brokerOrMarketmakerDetails>
        <name>Charles Schwab &amp; Co., Inc.</name>
        <address>
          <com:street1>3000 Schwab Way</com:street1>
          <com:city>Westlake</com:city>
          <com:stateOrCountry>TX</com:stateOrCountry>
          <com:zipCode>76262</com:zipCode>
        </address>
      </brokerOrMarketmakerDetails>
      <noOfUnitsSold>3000</noOfUnitsSold>
      <aggregateMarketValue>251597.00</aggregateMarketValue>
      <noOfUnitsOutstanding>8461494</noOfUnitsOutstanding>
      <approxSaleDate>04/29/2025</approxSaleDate>
      <securitiesExchangeName>Nasdaq</securitiesExchangeName>
    </securitiesInformation>
    <securitiesToBeSold>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <acquiredDate>09/20/2024</acquiredDate>
      <natureOfAcquisitionTransaction>Stock Option Exercise</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>3166</amountOfSecuritiesAcquired>
      <paymentDate>09/20/2024</paymentDate>
      <natureOfPayment>Cash</natureOfPayment>
    </securitiesToBeSold>
    <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <remarks>Late SEC 144 filing for 4/29/25 sale of 3,000 shares</remarks>
    <noticeSignature>
      <noticeDate>05/19/2025</noticeDate>
      <signature>Kevin C. Waterhouse</signature>
    </noticeSignature>
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144
0001710030
XXXXXXXX
LIVE
0001531031
Esquire Financial Holdings, Inc.
001-38131
100 JERICHO QUADRANGLE
SUITE 100
JERICHO
NY
11753
(800) 996-0213
Waterhouse Kevin C
Director
Common Stock
Charles Schwab & Co., Inc.
3000 Schwab Way
Westlake
TX
76262
3000
251597.00
8461494
04/29/2025
Nasdaq
Common
09/20/2024
Stock Option Exercise
Issuer
N
3166
09/20/2024
Cash
Y
Late SEC 144 filing for 4/29/25 sale of 3,000 shares
05/19/2025
Kevin C. Waterhouse