<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
  <headerData>
    <submissionType>144</submissionType>
    <filerInfo>
      <filer>
        <filerCredentials>
          <cik>0001780327</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>



    </filerInfo>
  </headerData>
  <formData>
    <issuerInfo>
      <issuerCik>0001755953</issuerCik>
      <issuerName>Akerna Corp.</issuerName>
      <secFileNumber>001-39096</secFileNumber>
      <issuerAddress>
        <com:street1>1550 LARIMER STREET, SUITE 246</com:street1>
        <com:city>DENVER</com:city>
        <com:stateOrCountry>CO</com:stateOrCountry>
        <com:zipCode>80202</com:zipCode>
      </issuerAddress>
      <issuerContactPhone>888-492-3540</issuerContactPhone>
      <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Kane Matthew Ryan</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <relationshipsToIssuer>
        <relationshipToIssuer>Director</relationshipToIssuer>
      </relationshipsToIssuer>
    </issuerInfo>
    <securitiesInformation>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <brokerOrMarketmakerDetails>
        <name>Charles Schwab Corp.</name>
        <address>
          <com:street1>3000 Schwab Way</com:street1>
          <com:city>Westlake</com:city>
          <com:stateOrCountry>TX</com:stateOrCountry>
          <com:zipCode>76262</com:zipCode>
        </address>
      </brokerOrMarketmakerDetails>
      <noOfUnitsSold>12823</noOfUnitsSold>
      <aggregateMarketValue>3769.00</aggregateMarketValue>
      <noOfUnitsOutstanding>10252018</noOfUnitsOutstanding>
      <approxSaleDate>12/21/2023</approxSaleDate>
      <securitiesExchangeName>Nasdaq</securitiesExchangeName>
    </securitiesInformation>
    <securitiesToBeSold>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <acquiredDate>09/06/2023</acquiredDate>
      <natureOfAcquisitionTransaction>Stock Grants</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>12823</amountOfSecuritiesAcquired>
      <paymentDate>09/06/2023</paymentDate>
      <natureOfPayment>N/A</natureOfPayment>
    </securitiesToBeSold>
    <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <remarks>Affiliate: Matthew Ryan Kane is the AGNT for Seam Capital LLC</remarks>
    <noticeSignature>
      <noticeDate>12/21/2023</noticeDate>
      <signature>Matthew Capital LLC</signature>
    </noticeSignature>
  </formData>
</edgarSubmission>
[{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 2777}]
144
0001780327
XXXXXXXX
LIVE
0001755953
Akerna Corp.
001-39096
1550 LARIMER STREET, SUITE 246
DENVER
CO
80202
888-492-3540
Kane Matthew Ryan
Director
Common
Charles Schwab Corp.
3000 Schwab Way
Westlake
TX
76262
12823
3769.00
10252018
12/21/2023
Nasdaq
Common
09/06/2023
Stock Grants
Issuer
N
12823
09/06/2023
N/A
Y
Affiliate: Matthew Ryan Kane is the AGNT for Seam Capital LLC
12/21/2023
Matthew Capital LLC