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      <secFileNumber>001-37806</secFileNumber>
      <issuerAddress>
        <com:street1>101 SPEAR STREET</com:street1>
        <com:street2>SUITE 500</com:street2>
        <com:city>San Francisco</com:city>
        <com:stateOrCountry>CA</com:stateOrCountry>
        <com:zipCode>94105</com:zipCode>
      </issuerAddress>
      <issuerContactPhone>415-390-2337</issuerContactPhone>
      <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Shipchandler Khozema</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <relationshipsToIssuer>
        <relationshipToIssuer>Officer</relationshipToIssuer>
      </relationshipsToIssuer>
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    <securitiesInformation>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <brokerOrMarketmakerDetails>
        <name>Charles Schwab &amp; Co., Inc.</name>
        <address>
          <com:street1>3000 Schwab Way</com:street1>
          <com:city>Westlake</com:city>
          <com:stateOrCountry>TX</com:stateOrCountry>
          <com:zipCode>76262</com:zipCode>
        </address>
      </brokerOrMarketmakerDetails>
      <noOfUnitsSold>11044</noOfUnitsSold>
      <aggregateMarketValue>731043.00</aggregateMarketValue>
      <noOfUnitsOutstanding>160599847</noOfUnitsOutstanding>
      <approxSaleDate>10/03/2024</approxSaleDate>
      <securitiesExchangeName>NYSE</securitiesExchangeName>
    </securitiesInformation>
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      <securitiesClassTitle>Common</securitiesClassTitle>
      <acquiredDate>09/30/2024</acquiredDate>
      <natureOfAcquisitionTransaction>Restricted Stock Lapse</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>TWILIO INC.</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>11044</amountOfSecuritiesAcquired>
      <paymentDate>09/30/2024</paymentDate>
      <natureOfPayment>Equity Compensation</natureOfPayment>
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    <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
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        <name>Khozema Shipchandler</name>
        <address>
          <com:street1>101 SPEAR STREET, SUITE 500</com:street1>
          <com:city>SAN FRANCISCO</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94105</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>TWILIO INC</securitiesClassTitle>
      <saleDate>07/05/2024</saleDate>
      <amountOfSecuritiesSold>10877</amountOfSecuritiesSold>
      <grossProceeds>611940.00</grossProceeds>
    </securitiesSoldInPast3Months>
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      <sellerDetails>
        <name>Khozema Shipchandler</name>
        <address>
          <com:street1>101 SPEAR STREET, SUITE 500</com:street1>
          <com:city>SAN FRANCISCO</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94105</com:zipCode>
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      <securitiesClassTitle>TWILIO INC</securitiesClassTitle>
      <saleDate>09/30/2024</saleDate>
      <amountOfSecuritiesSold>11073</amountOfSecuritiesSold>
      <grossProceeds>720117.00</grossProceeds>
    </securitiesSoldInPast3Months>
    <noticeSignature>
      <noticeDate>10/03/2024</noticeDate>
      <planAdoptionDates>
        <planAdoptionDate>03/06/2024</planAdoptionDate>
      </planAdoptionDates>
      <signature>Khozema Shipchandler</signature>
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144
0001737610
XXXXXXXX
LIVE
0001447669
TWILIO INC
001-37806
101 SPEAR STREET
SUITE 500
San Francisco
CA
94105
415-390-2337
Shipchandler Khozema
Officer
Common
Charles Schwab & Co., Inc.
3000 Schwab Way
Westlake
TX
76262
11044
731043.00
160599847
10/03/2024
NYSE
Common
09/30/2024
Restricted Stock Lapse
TWILIO INC.
N
11044
09/30/2024
Equity Compensation
N
Khozema Shipchandler
101 SPEAR STREET, SUITE 500
SAN FRANCISCO
CA
94105
TWILIO INC
07/05/2024
10877
611940.00
Khozema Shipchandler
101 SPEAR STREET, SUITE 500
SAN FRANCISCO
CA
94105
TWILIO INC
09/30/2024
11073
720117.00
10/03/2024
03/06/2024
Khozema Shipchandler