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            <issuerCik>0001831840</issuerCik>
            <issuerName>SEMRUSH HOLDINGS, INC.</issuerName>
            <secFileNumber>001-40276</secFileNumber>
            <issuerAddress>
                <ns2:street1>800 BOYLSTON STREET, SUITE 2475</ns2:street1>
                <ns2:city>BOSTON</ns2:city>
                <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                <ns2:zipCode>02199</ns2:zipCode>
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            <issuerContactPhone>800-851-9959</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>DMITRY MELNIKOV</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>Director</relationshipToIssuer>
            </relationshipsToIssuer>
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        <securitiesInformation>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>38th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
                </address>
            </brokerOrMarketmakerDetails>
            <noOfUnitsSold>500000</noOfUnitsSold>
            <aggregateMarketValue>3625000.00</aggregateMarketValue>
            <noOfUnitsOutstanding>118376410</noOfUnitsOutstanding>
            <approxSaleDate>06/06/2023</approxSaleDate>
            <securitiesExchangeName>NYSE</securitiesExchangeName>
        </securitiesInformation>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>03/15/2021</acquiredDate>
            <natureOfAcquisitionTransaction>Founder Stock</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>500000</amountOfSecuritiesAcquired>
            <paymentDate>03/15/2021</paymentDate>
            <natureOfPayment>Services Rendered</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <noticeSignature>
            <noticeDate>06/06/2023</noticeDate>
            <planAdoptionDates/>
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144
0001849699
XXXXXXXX
LIVE
0001831840
SEMRUSH HOLDINGS, INC.
001-40276
800 BOYLSTON STREET, SUITE 2475
BOSTON
MA
02199
800-851-9959
DMITRY MELNIKOV
Director
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
38th Floor
New York
NY
10004
500000
3625000.00
118376410
06/06/2023
NYSE
Common
03/15/2021
Founder Stock
Issuer
N
500000
03/15/2021
Services Rendered
Y
06/06/2023
/s/ Dmitry Melnikov