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      <issuerCik>0000855683</issuerCik>
      <issuerName>MILESTONE SCIENTIFIC INC.</issuerName>
      <secFileNumber>001-14053</secFileNumber>
      <issuerAddress>
        <com:street1>425 EAGLE ROCK AVENUE</com:street1>
        <com:street2>SUITE 403</com:street2>
        <com:city>ROSELAND</com:city>
        <com:stateOrCountry>NJ</com:stateOrCountry>
        <com:zipCode>07068</com:zipCode>
      </issuerAddress>
      <issuerContactPhone>(973) 535-2717</issuerContactPhone>
      <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>McGeehan Michael</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <relationshipsToIssuer>
        <relationshipToIssuer>Director</relationshipToIssuer>
      </relationshipsToIssuer>
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    <securitiesInformation>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <brokerOrMarketmakerDetails>
        <name>Charles Schwab Corp.</name>
        <address>
          <com:street1>3000 Schwab Way</com:street1>
          <com:city>Westkaje</com:city>
          <com:stateOrCountry>TX</com:stateOrCountry>
          <com:zipCode>76262</com:zipCode>
        </address>
      </brokerOrMarketmakerDetails>
      <noOfUnitsSold>50107</noOfUnitsSold>
      <aggregateMarketValue>48102.72</aggregateMarketValue>
      <noOfUnitsOutstanding>77287714</noOfUnitsOutstanding>
      <approxSaleDate>09/11/2024</approxSaleDate>
      <securitiesExchangeName>NYSE</securitiesExchangeName>
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      <securitiesClassTitle>Common</securitiesClassTitle>
      <acquiredDate>07/16/2021</acquiredDate>
      <natureOfAcquisitionTransaction>Compensation</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>50107</amountOfSecuritiesAcquired>
      <paymentDate>07/16/2021</paymentDate>
      <natureOfPayment>N/A</natureOfPayment>
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    <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <noticeSignature>
      <noticeDate>09/11/2024</noticeDate>
      <signature>Michael McGeehan</signature>
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144
0001768936
XXXXXXXX
LIVE
0000855683
MILESTONE SCIENTIFIC INC.
001-14053
425 EAGLE ROCK AVENUE
SUITE 403
ROSELAND
NJ
07068
(973) 535-2717
McGeehan Michael
Director
Common
Charles Schwab Corp.
3000 Schwab Way
Westkaje
TX
76262
50107
48102.72
77287714
09/11/2024
NYSE
Common
07/16/2021
Compensation
Issuer
N
50107
07/16/2021
N/A
Y
09/11/2024
Michael McGeehan