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        <filerInfo>
            <filer>
                <filerCredentials>
                    <cik>0001857447</cik>
                    <ccc>XXXXXXXX</ccc>
                </filerCredentials>
            </filer>
            <liveTestFlag>LIVE</liveTestFlag>



        </filerInfo>
    </headerData>
    <formData>
        <issuerInfo>
            <issuerCik>0001711933</issuerCik>
            <issuerName>AKOYA BIOSCIENCES, INC.</issuerName>
            <secFileNumber>001-40344</secFileNumber>
            <issuerAddress>
                <ns2:street1>100 Campus Drive, 6th Floor</ns2:street1>
                <ns2:city>Marlborough</ns2:city>
                <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                <ns2:zipCode>01752</ns2:zipCode>
            </issuerAddress>
            <issuerContactPhone>(855) 896-8401</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>BRIAN M MCKELLIGON</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>Officer</relationshipToIssuer>
                <relationshipToIssuer>Director</relationshipToIssuer>
            </relationshipsToIssuer>
        </issuerInfo>
        <securitiesInformation>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
                </address>
            </brokerOrMarketmakerDetails>
            <noOfUnitsSold>7500</noOfUnitsSold>
            <aggregateMarketValue>37242.75</aggregateMarketValue>
            <noOfUnitsOutstanding>49140731</noOfUnitsOutstanding>
            <approxSaleDate>03/20/2024</approxSaleDate>
            <securitiesExchangeName>NASDAQ</securitiesExchangeName>
        </securitiesInformation>
        <securitiesToBeSold>
            <securitiesClassTitle>COMMON</securitiesClassTitle>
            <acquiredDate>03/20/2024</acquiredDate>
            <natureOfAcquisitionTransaction>Stock Option Exercise</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>ISSUER</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>7500</amountOfSecuritiesAcquired>
            <paymentDate>03/20/2024</paymentDate>
            <natureOfPayment>Cash</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>BRIAN M MCKELLIGON</name>
                <address>
                    <ns2:street1>100 Campus Drive, 6th Floor</ns2:street1>
                    <ns2:city>Marlborough</ns2:city>
                    <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                    <ns2:zipCode>01752</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/13/2024</saleDate>
            <amountOfSecuritiesSold>7500</amountOfSecuritiesSold>
            <grossProceeds>37041.00</grossProceeds>
        </securitiesSoldInPast3Months>
        <noticeSignature>
            <noticeDate>03/20/2024</noticeDate>
            <planAdoptionDates>
                <planAdoptionDate>08/16/2023</planAdoptionDate>
            </planAdoptionDates>
            <signature>/s/ Brian McKelligon</signature>
        </noticeSignature>
    </formData>
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144
0001857447
XXXXXXXX
LIVE
0001711933
AKOYA BIOSCIENCES, INC.
001-40344
100 Campus Drive, 6th Floor
Marlborough
MA
01752
(855) 896-8401
BRIAN M MCKELLIGON
Officer
Director
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
8th Floor
New York
NY
10004
7500
37242.75
49140731
03/20/2024
NASDAQ
COMMON
03/20/2024
Stock Option Exercise
ISSUER
N
7500
03/20/2024
Cash
N
BRIAN M MCKELLIGON
100 Campus Drive, 6th Floor
Marlborough
MA
01752
Common
03/13/2024
7500
37041.00
03/20/2024
08/16/2023
/s/ Brian McKelligon