<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:ns2="http://www.sec.gov/edgar/common">
    <headerData>
        <submissionType>144</submissionType>
        <filerInfo>
            <filer>
                <filerCredentials>
                    <cik>0001283212</cik>
                    <ccc>XXXXXXXX</ccc>
                </filerCredentials>
            </filer>
            <liveTestFlag>LIVE</liveTestFlag>



        </filerInfo>
    </headerData>
    <formData>
        <issuerInfo>
            <issuerCik>0001124524</issuerCik>
            <issuerName>CRYOPORT, INC.</issuerName>
            <secFileNumber>001-34632</secFileNumber>
            <issuerAddress>
                <ns2:street1>112 Westwood Place, Suite 350</ns2:street1>
                <ns2:city>Brentwood</ns2:city>
                <ns2:stateOrCountry>TN</ns2:stateOrCountry>
                <ns2:zipCode>37027</ns2:zipCode>
            </issuerAddress>
            <issuerContactPhone>949-470-2300</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>JERRELL W SHELTON</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>Director</relationshipToIssuer>
                <relationshipToIssuer>Officer</relationshipToIssuer>
            </relationshipsToIssuer>
        </issuerInfo>
        <securitiesInformation>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
                </address>
            </brokerOrMarketmakerDetails>
            <noOfUnitsSold>356064</noOfUnitsSold>
            <aggregateMarketValue>2467523.52</aggregateMarketValue>
            <noOfUnitsOutstanding>50137218</noOfUnitsOutstanding>
            <approxSaleDate>06/17/2025</approxSaleDate>
            <securitiesExchangeName>NASDAQ</securitiesExchangeName>
        </securitiesInformation>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>06/17/2025</acquiredDate>
            <natureOfAcquisitionTransaction>Exercise of Stock Options</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>356064</amountOfSecuritiesAcquired>
            <paymentDate>06/17/2025</paymentDate>
            <natureOfPayment>Cash</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>JERRELL W SHELTON</name>
                <address>
                    <ns2:street1>112 Westwood Place, Suite 350</ns2:street1>
                    <ns2:city>Brentwood</ns2:city>
                    <ns2:stateOrCountry>TN</ns2:stateOrCountry>
                    <ns2:zipCode>37027</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/24/2025</saleDate>
            <amountOfSecuritiesSold>2980</amountOfSecuritiesSold>
            <grossProceeds>20685.97</grossProceeds>
        </securitiesSoldInPast3Months>
        <remarks>The sale of shares covered by this Form 144 were made pursuant to a pre-set sales plan adopted by the seller on 6/3/2025.  The seller signed this Form 144 on 6/3/2025 pursuant to such pre-set sales plan and made the below representation as of such plan adoption date.  </remarks>
        <noticeSignature>
            <noticeDate>06/17/2025</noticeDate>
            <planAdoptionDates/>
            <signature>/s/ Jerrell W Shelton</signature>
        </noticeSignature>
    </formData>
</edgarSubmission>
[{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 4295}]
144
0001283212
XXXXXXXX
LIVE
0001124524
CRYOPORT, INC.
001-34632
112 Westwood Place, Suite 350
Brentwood
TN
37027
949-470-2300
JERRELL W SHELTON
Director
Officer
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
8th Floor
New York
NY
10004
356064
2467523.52
50137218
06/17/2025
NASDAQ
Common
06/17/2025
Exercise of Stock Options
Issuer
N
356064
06/17/2025
Cash
N
JERRELL W SHELTON
112 Westwood Place, Suite 350
Brentwood
TN
37027
Common
03/24/2025
2980
20685.97
The sale of shares covered by this Form 144 were made pursuant to a pre-set sales plan adopted by the seller on 6/3/2025.  The seller signed this Form 144 on 6/3/2025 pursuant to such pre-set sales plan and made the below representation as of such plan adoption date.
06/17/2025
/s/ Jerrell W Shelton