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    <headerData>
        <submissionType>144</submissionType>
        <filerInfo>
            <filer>
                <filerCredentials>
                    <cik>0001841662</cik>
                    <ccc>XXXXXXXX</ccc>
                </filerCredentials>
            </filer>
            <liveTestFlag>LIVE</liveTestFlag>



        </filerInfo>
    </headerData>
    <formData>
        <issuerInfo>
            <issuerCik>0001110611</issuerCik>
            <issuerName>ON24, INC.</issuerName>
            <secFileNumber>001-39965</secFileNumber>
            <issuerAddress>
                <ns2:street1>50 Beale Street</ns2:street1>
                <ns2:street2>8th Floor</ns2:street2>
                <ns2:city>San Francisco</ns2:city>
                <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                <ns2:zipCode>94105</ns2:zipCode>
            </issuerAddress>
            <issuerContactPhone>(415) 369-8000</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>JAYESH SAHASI</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>Officer</relationshipToIssuer>
            </relationshipsToIssuer>
        </issuerInfo>
        <securitiesInformation>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
                </address>
            </brokerOrMarketmakerDetails>
            <noOfUnitsSold>26994</noOfUnitsSold>
            <aggregateMarketValue>152254.26</aggregateMarketValue>
            <noOfUnitsOutstanding>42475166</noOfUnitsOutstanding>
            <approxSaleDate>06/02/2025</approxSaleDate>
            <securitiesExchangeName>NYSE</securitiesExchangeName>
        </securitiesInformation>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>06/01/2025</acquiredDate>
            <natureOfAcquisitionTransaction>Restricted Stock</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>26994</amountOfSecuritiesAcquired>
            <paymentDate>06/01/2025</paymentDate>
            <natureOfPayment>Not Applicable</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>JAYESH SAHASI</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>San Francisco</ns2:city>
                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>05/20/2025</saleDate>
            <amountOfSecuritiesSold>2888</amountOfSecuritiesSold>
            <grossProceeds>15722.85</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>JAYESH SAHASI</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>San Francisco</ns2:city>
                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/20/2025</saleDate>
            <amountOfSecuritiesSold>3696</amountOfSecuritiesSold>
            <grossProceeds>20009.04</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>JAYESH SAHASI</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>San Francisco</ns2:city>
                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/19/2025</saleDate>
            <amountOfSecuritiesSold>71317</amountOfSecuritiesSold>
            <grossProceeds>391637.31</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>JAYESH SAHASI</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>San Francisco</ns2:city>
                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/18/2025</saleDate>
            <amountOfSecuritiesSold>35564</amountOfSecuritiesSold>
            <grossProceeds>194876.49</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>JAYESH SAHASI</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>San Francisco</ns2:city>
                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/04/2025</saleDate>
            <amountOfSecuritiesSold>4322</amountOfSecuritiesSold>
            <grossProceeds>23884.67</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>JAYESH SAHASI</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>San Francisco</ns2:city>
                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/03/2025</saleDate>
            <amountOfSecuritiesSold>7095</amountOfSecuritiesSold>
            <grossProceeds>39526.25</grossProceeds>
        </securitiesSoldInPast3Months>
        <noticeSignature>
            <noticeDate>06/02/2025</noticeDate>
            <planAdoptionDates/>
            <signature>/s/ Jayesh Sahasi</signature>
        </noticeSignature>
    </formData>
</edgarSubmission>
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144
0001841662
XXXXXXXX
LIVE
0001110611
ON24, INC.
001-39965
50 Beale Street
8th Floor
San Francisco
CA
94105
(415) 369-8000
JAYESH SAHASI
Officer
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
8th Floor
New York
NY
10004
26994
152254.26
42475166
06/02/2025
NYSE
Common
06/01/2025
Restricted Stock
Issuer
N
26994
06/01/2025
Not Applicable
N
JAYESH SAHASI
50 Beale Street
8th Floor
San Francisco
CA
94105
Common
05/20/2025
2888
15722.85
JAYESH SAHASI
50 Beale Street
8th Floor
San Francisco
CA
94105
Common
03/20/2025
3696
20009.04
JAYESH SAHASI
50 Beale Street
8th Floor
San Francisco
CA
94105
Common
03/19/2025
71317
391637.31
JAYESH SAHASI
50 Beale Street
8th Floor
San Francisco
CA
94105
Common
03/18/2025
35564
194876.49
JAYESH SAHASI
50 Beale Street
8th Floor
San Francisco
CA
94105
Common
03/04/2025
4322
23884.67
JAYESH SAHASI
50 Beale Street
8th Floor
San Francisco
CA
94105
Common
03/03/2025
7095
39526.25
06/02/2025
/s/ Jayesh Sahasi