<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
  <headerData>
    <submissionType>144</submissionType>
    <filerInfo>
      <filer>
        <filerCredentials>
          <cik>0001319892</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>



    </filerInfo>
  </headerData>
  <formData>
    <issuerInfo>
      <issuerCik>0001494650</issuerCik>
      <issuerName>OptiNose, Inc.</issuerName>
      <secFileNumber>001-38241</secFileNumber>
      <issuerAddress>
        <com:street1>1020 STONY HILL ROAD</com:street1>
        <com:street2>SUITE 300</com:street2>
        <com:city>YARDLEY</com:city>
        <com:stateOrCountry>PA</com:stateOrCountry>
        <com:zipCode>19067</com:zipCode>
      </issuerAddress>
      <issuerContactPhone>267-364-3500</issuerContactPhone>
      <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Scodari Joseph C</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <relationshipsToIssuer>
        <relationshipToIssuer>former affiliate</relationshipToIssuer>
      </relationshipsToIssuer>
    </issuerInfo>
    <securitiesInformation>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <brokerOrMarketmakerDetails>
        <name>Charles Schwab &amp; Co., Inc.</name>
        <address>
          <com:street1>3000 Schwab Way</com:street1>
          <com:city>Westlake</com:city>
          <com:stateOrCountry>TX</com:stateOrCountry>
          <com:zipCode>76262</com:zipCode>
        </address>
      </brokerOrMarketmakerDetails>
      <noOfUnitsSold>24800</noOfUnitsSold>
      <aggregateMarketValue>31000.00</aggregateMarketValue>
      <noOfUnitsOutstanding>112223317</noOfUnitsOutstanding>
      <approxSaleDate>08/18/2023</approxSaleDate>
      <securitiesExchangeName>Nasdaq</securitiesExchangeName>
    </securitiesInformation>
    <securitiesToBeSold>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <acquiredDate>12/06/2019</acquiredDate>
      <natureOfAcquisitionTransaction>Open market purchase</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>open market</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>24800</amountOfSecuritiesAcquired>
      <paymentDate>12/06/2019</paymentDate>
      <natureOfPayment>Cash</natureOfPayment>
    </securitiesToBeSold>
    <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Joseph C. Scodari</name>
        <address>
          <com:street1>3826 Meadow View Farm Road</com:street1>
          <com:city>Newton Square</com:city>
          <com:stateOrCountry>PA</com:stateOrCountry>
          <com:zipCode>19073</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <saleDate>08/14/2023</saleDate>
      <amountOfSecuritiesSold>6430</amountOfSecuritiesSold>
      <grossProceeds>7779.30</grossProceeds>
    </securitiesSoldInPast3Months>
    <remarks>date of shares acquired 12/06/2019 - 05/10/2021 open market purchase date of payment 12/06/2019 - 5/10/2021 Name of person for whose account the securities are to be sold: Joseph C. Scodari TTEE, The Joseph C. Scodari Rev Trust dtd 4/11/2016</remarks>
    <noticeSignature>
      <noticeDate>08/18/2023</noticeDate>
      <signature>Joseph C Scodari, TTEE The Joseph C. Scodari Rev Tr 4/11/2016</signature>
    </noticeSignature>
  </formData>
</edgarSubmission>
[{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 3667}]
144
0001319892
XXXXXXXX
LIVE
0001494650
OptiNose, Inc.
001-38241
1020 STONY HILL ROAD
SUITE 300
YARDLEY
PA
19067
267-364-3500
Scodari Joseph C
former affiliate
Common
Charles Schwab & Co., Inc.
3000 Schwab Way
Westlake
TX
76262
24800
31000.00
112223317
08/18/2023
Nasdaq
Common
12/06/2019
Open market purchase
open market
N
24800
12/06/2019
Cash
N
Joseph C. Scodari
3826 Meadow View Farm Road
Newton Square
PA
19073
Common
08/14/2023
6430
7779.30
date of shares acquired 12/06/2019 - 05/10/2021 open market purchase date of payment 12/06/2019 - 5/10/2021 Name of person for whose account the securities are to be sold: Joseph C. Scodari TTEE, The Joseph C. Scodari Rev Trust dtd 4/11/2016
08/18/2023
Joseph C Scodari, TTEE The Joseph C. Scodari Rev Tr 4/11/2016