<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:ns2="http://www.sec.gov/edgar/common">
    <headerData>
        <submissionType>144</submissionType>
        <filerInfo>
            <filer>
                <filerCredentials>
                    <cik>0001818261</cik>
                    <ccc>XXXXXXXX</ccc>
                </filerCredentials>
            </filer>
            <liveTestFlag>LIVE</liveTestFlag>



        </filerInfo>
    </headerData>
    <formData>
        <issuerInfo>
            <issuerCik>0001812364</issuerCik>
            <issuerName>RELAY THERAPEUTICS, INC.</issuerName>
            <secFileNumber>001-39385</secFileNumber>
            <issuerAddress>
                <ns2:street1>399 Binney Street</ns2:street1>
                <ns2:street2>2nd Floor</ns2:street2>
                <ns2:city>Cambridge</ns2:city>
                <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                <ns2:zipCode>02142</ns2:zipCode>
            </issuerAddress>
            <issuerContactPhone>(617)  370-8837</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>THOMAS J CATINAZZO</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>Officer</relationshipToIssuer>
            </relationshipsToIssuer>
        </issuerInfo>
        <securitiesInformation>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
                </address>
            </brokerOrMarketmakerDetails>
            <noOfUnitsSold>3558</noOfUnitsSold>
            <aggregateMarketValue>11492.34</aggregateMarketValue>
            <noOfUnitsOutstanding>170488817</noOfUnitsOutstanding>
            <approxSaleDate>04/29/2025</approxSaleDate>
            <securitiesExchangeName>NASDAQ</securitiesExchangeName>
        </securitiesInformation>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>04/27/2025</acquiredDate>
            <natureOfAcquisitionTransaction>Restricted Stock</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>3558</amountOfSecuritiesAcquired>
            <paymentDate>04/27/2025</paymentDate>
            <natureOfPayment>Not Applicable</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>THOMAS J CATINAZZO</name>
                <address>
                    <ns2:street1>399 Binney Street</ns2:street1>
                    <ns2:street2>2nd Floor</ns2:street2>
                    <ns2:city>Cambridge</ns2:city>
                    <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                    <ns2:zipCode>02142</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>04/28/2025</saleDate>
            <amountOfSecuritiesSold>1709</amountOfSecuritiesSold>
            <grossProceeds>5417.71</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>THOMAS J CATINAZZO</name>
                <address>
                    <ns2:street1>399 Binney Street</ns2:street1>
                    <ns2:street2>2nd Floor</ns2:street2>
                    <ns2:city>Cambridge</ns2:city>
                    <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                    <ns2:zipCode>02142</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>04/28/2025</saleDate>
            <amountOfSecuritiesSold>54</amountOfSecuritiesSold>
            <grossProceeds>171.18</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>THOMAS J CATINAZZO</name>
                <address>
                    <ns2:street1>399 Binney Street</ns2:street1>
                    <ns2:street2>2nd Floor</ns2:street2>
                    <ns2:city>Cambridge</ns2:city>
                    <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                    <ns2:zipCode>02142</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>04/28/2025</saleDate>
            <amountOfSecuritiesSold>321</amountOfSecuritiesSold>
            <grossProceeds>1017.57</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>THOMAS J CATINAZZO</name>
                <address>
                    <ns2:street1>399 Binney Street</ns2:street1>
                    <ns2:street2>2nd Floor</ns2:street2>
                    <ns2:city>Cambridge</ns2:city>
                    <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                    <ns2:zipCode>02142</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>04/28/2025</saleDate>
            <amountOfSecuritiesSold>606</amountOfSecuritiesSold>
            <grossProceeds>1921.02</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>THOMAS J CATINAZZO</name>
                <address>
                    <ns2:street1>399 Binney Street</ns2:street1>
                    <ns2:street2>2nd Floor</ns2:street2>
                    <ns2:city>Cambridge</ns2:city>
                    <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                    <ns2:zipCode>02142</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/27/2025</saleDate>
            <amountOfSecuritiesSold>298</amountOfSecuritiesSold>
            <grossProceeds>846.32</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>THOMAS J CATINAZZO</name>
                <address>
                    <ns2:street1>399 Binney Street</ns2:street1>
                    <ns2:street2>2nd Floor</ns2:street2>
                    <ns2:city>Cambridge</ns2:city>
                    <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                    <ns2:zipCode>02142</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/27/2025</saleDate>
            <amountOfSecuritiesSold>1262</amountOfSecuritiesSold>
            <grossProceeds>3584.08</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>THOMAS J CATINAZZO</name>
                <address>
                    <ns2:street1>399 Binney Street</ns2:street1>
                    <ns2:street2>2nd Floor</ns2:street2>
                    <ns2:city>Cambridge</ns2:city>
                    <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                    <ns2:zipCode>02142</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/27/2025</saleDate>
            <amountOfSecuritiesSold>1727</amountOfSecuritiesSold>
            <grossProceeds>4904.68</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>THOMAS J CATINAZZO</name>
                <address>
                    <ns2:street1>399 Binney Street</ns2:street1>
                    <ns2:street2>2nd Floor</ns2:street2>
                    <ns2:city>Cambridge</ns2:city>
                    <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                    <ns2:zipCode>02142</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/27/2025</saleDate>
            <amountOfSecuritiesSold>257</amountOfSecuritiesSold>
            <grossProceeds>729.88</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>THOMAS J CATINAZZO</name>
                <address>
                    <ns2:street1>399 Binney Street</ns2:street1>
                    <ns2:street2>2nd Floor</ns2:street2>
                    <ns2:city>Cambridge</ns2:city>
                    <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                    <ns2:zipCode>02142</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/27/2025</saleDate>
            <amountOfSecuritiesSold>590</amountOfSecuritiesSold>
            <grossProceeds>1675.60</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>THOMAS J CATINAZZO</name>
                <address>
                    <ns2:street1>399 Binney Street</ns2:street1>
                    <ns2:street2>2nd Floor</ns2:street2>
                    <ns2:city>Cambridge</ns2:city>
                    <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                    <ns2:zipCode>02142</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/27/2025</saleDate>
            <amountOfSecuritiesSold>698</amountOfSecuritiesSold>
            <grossProceeds>1982.32</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>THOMAS J CATINAZZO</name>
                <address>
                    <ns2:street1>399 Binney Street</ns2:street1>
                    <ns2:street2>2nd Floor</ns2:street2>
                    <ns2:city>Cambridge</ns2:city>
                    <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                    <ns2:zipCode>02142</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/27/2025</saleDate>
            <amountOfSecuritiesSold>32</amountOfSecuritiesSold>
            <grossProceeds>90.88</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>THOMAS J CATINAZZO</name>
                <address>
                    <ns2:street1>399 Binney Street</ns2:street1>
                    <ns2:street2>2nd Floor</ns2:street2>
                    <ns2:city>Cambridge</ns2:city>
                    <ns2:stateOrCountry>MA</ns2:stateOrCountry>
                    <ns2:zipCode>02142</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>01/30/2025</saleDate>
            <amountOfSecuritiesSold>20791</amountOfSecuritiesSold>
            <grossProceeds>91912.06</grossProceeds>
        </securitiesSoldInPast3Months>
        <noticeSignature>
            <noticeDate>04/29/2025</noticeDate>
            <planAdoptionDates>
                <planAdoptionDate>02/15/2024</planAdoptionDate>
            </planAdoptionDates>
            <signature>/s/ Thomas Catinazzo</signature>
        </noticeSignature>
    </formData>
</edgarSubmission>
[{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 12356}]
144
0001818261
XXXXXXXX
LIVE
0001812364
RELAY THERAPEUTICS, INC.
001-39385
399 Binney Street
2nd Floor
Cambridge
MA
02142
(617)  370-8837
THOMAS J CATINAZZO
Officer
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
8th Floor
New York
NY
10004
3558
11492.34
170488817
04/29/2025
NASDAQ
Common
04/27/2025
Restricted Stock
Issuer
N
3558
04/27/2025
Not Applicable
N
THOMAS J CATINAZZO
399 Binney Street
2nd Floor
Cambridge
MA
02142
Common
04/28/2025
1709
5417.71
THOMAS J CATINAZZO
399 Binney Street
2nd Floor
Cambridge
MA
02142
Common
04/28/2025
54
171.18
THOMAS J CATINAZZO
399 Binney Street
2nd Floor
Cambridge
MA
02142
Common
04/28/2025
321
1017.57
THOMAS J CATINAZZO
399 Binney Street
2nd Floor
Cambridge
MA
02142
Common
04/28/2025
606
1921.02
THOMAS J CATINAZZO
399 Binney Street
2nd Floor
Cambridge
MA
02142
Common
03/27/2025
298
846.32
THOMAS J CATINAZZO
399 Binney Street
2nd Floor
Cambridge
MA
02142
Common
03/27/2025
1262
3584.08
THOMAS J CATINAZZO
399 Binney Street
2nd Floor
Cambridge
MA
02142
Common
03/27/2025
1727
4904.68
THOMAS J CATINAZZO
399 Binney Street
2nd Floor
Cambridge
MA
02142
Common
03/27/2025
257
729.88
THOMAS J CATINAZZO
399 Binney Street
2nd Floor
Cambridge
MA
02142
Common
03/27/2025
590
1675.60
THOMAS J CATINAZZO
399 Binney Street
2nd Floor
Cambridge
MA
02142
Common
03/27/2025
698
1982.32
THOMAS J CATINAZZO
399 Binney Street
2nd Floor
Cambridge
MA
02142
Common
03/27/2025
32
90.88
THOMAS J CATINAZZO
399 Binney Street
2nd Floor
Cambridge
MA
02142
Common
01/30/2025
20791
91912.06
04/29/2025
02/15/2024
/s/ Thomas Catinazzo