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          <ccc>XXXXXXXX</ccc>
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      <liveTestFlag>LIVE</liveTestFlag>



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    <issuerInfo>
      <issuerCik>0001768224</issuerCik>
      <issuerName>Arcturus Therapeutics Holdings Inc.</issuerName>
      <secFileNumber>001-38942</secFileNumber>
      <issuerAddress>
        <com:street1>10628 SCIENCE CENTER DRIVE</com:street1>
        <com:street2>SUITE 250</com:street2>
        <com:city>SAN DIEGO</com:city>
        <com:stateOrCountry>CA</com:stateOrCountry>
        <com:zipCode>92121</com:zipCode>
      </issuerAddress>
      <issuerContactPhone>8589002660</issuerContactPhone>
      <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>SASSINE ANDY</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <relationshipsToIssuer>
        <relationshipToIssuer>Director</relationshipToIssuer>
      </relationshipsToIssuer>
    </issuerInfo>
    <securitiesInformation>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <brokerOrMarketmakerDetails>
        <name>Charles Schwab Corp.</name>
        <address>
          <com:street1>3000 Schwab Way</com:street1>
          <com:city>Westlake</com:city>
          <com:stateOrCountry>TX</com:stateOrCountry>
          <com:zipCode>76262</com:zipCode>
        </address>
      </brokerOrMarketmakerDetails>
      <noOfUnitsSold>50000</noOfUnitsSold>
      <aggregateMarketValue>1601500.00</aggregateMarketValue>
      <noOfUnitsOutstanding>26931826</noOfUnitsOutstanding>
      <approxSaleDate>06/12/2024</approxSaleDate>
      <securitiesExchangeName>NASDAQ</securitiesExchangeName>
    </securitiesInformation>
    <securitiesToBeSold>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <acquiredDate>02/13/2018</acquiredDate>
      <natureOfAcquisitionTransaction>Market Purchase</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>Open Market</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>50000</amountOfSecuritiesAcquired>
      <paymentDate>02/13/2018</paymentDate>
      <natureOfPayment>cash</natureOfPayment>
    </securitiesToBeSold>
    <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <remarks>Date range of purchase 2/13/2018 to 3/12/2018</remarks>
    <noticeSignature>
      <noticeDate>06/12/2024</noticeDate>
      <signature>ANDY H SASSINE</signature>
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144
0001515322
XXXXXXXX
LIVE
0001768224
Arcturus Therapeutics Holdings Inc.
001-38942
10628 SCIENCE CENTER DRIVE
SUITE 250
SAN DIEGO
CA
92121
8589002660
SASSINE ANDY
Director
Common
Charles Schwab Corp.
3000 Schwab Way
Westlake
TX
76262
50000
1601500.00
26931826
06/12/2024
NASDAQ
Common
02/13/2018
Market Purchase
Open Market
N
50000
02/13/2018
cash
Y
Date range of purchase 2/13/2018 to 3/12/2018
06/12/2024
ANDY H SASSINE