<?xml version="1.0" encoding="UTF-8"?><ns2:edgarSubmission xmlns:com="http://www.sec.gov/edgar/common" xmlns:ns2="http://www.sec.gov/edgar/ownership">
    <ns2:headerData>
        <ns2:submissionType>144</ns2:submissionType>
        <ns2:filerInfo>
            <ns2:filer>
                <ns2:filerCredentials>
                    <ns2:cik>0001236397</ns2:cik>
                    <ns2:ccc>XXXXXXXX</ns2:ccc>
                </ns2:filerCredentials>
            </ns2:filer>
            <ns2:liveTestFlag>LIVE</ns2:liveTestFlag>



        </ns2:filerInfo>
    </ns2:headerData>
    <ns2:formData>
        <ns2:issuerInfo>
            <ns2:issuerCik>0001447362</ns2:issuerCik>
            <ns2:issuerName>Castle Biosciences, Inc.</ns2:issuerName>
            <ns2:secFileNumber>001-38984</ns2:secFileNumber>
            <ns2:issuerAddress>
                <com:street1>505 S FRIENDSWOOD DR</com:street1>
                <com:street2>SUITE 401</com:street2>
                <com:city>FRIENDSWOOD</com:city>
                <com:stateOrCountry>TX</com:stateOrCountry>
                <com:zipCode>77546</com:zipCode>
            </ns2:issuerAddress>
            <ns2:issuerContactPhone>1-866-788-9007</ns2:issuerContactPhone>
            <ns2:nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Daniel Bradbury</ns2:nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <ns2:relationshipsToIssuer>
                <ns2:relationshipToIssuer>Officer</ns2:relationshipToIssuer>
            </ns2:relationshipsToIssuer>
        </ns2:issuerInfo>
        <ns2:securitiesInformation>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:brokerOrMarketmakerDetails>
                <ns2:name>Goldman Sachs &amp; Co. LLC</ns2:name>
                <ns2:address>
                    <com:street1>200 West Street</com:street1>
                    <com:city>New York</com:city>
                    <com:stateOrCountry>NY</com:stateOrCountry>
                    <com:zipCode>10282</com:zipCode>
                </ns2:address>
            </ns2:brokerOrMarketmakerDetails>
            <ns2:noOfUnitsSold>15750</ns2:noOfUnitsSold>
            <ns2:aggregateMarketValue>421312.5</ns2:aggregateMarketValue>
            <ns2:noOfUnitsOutstanding>27736760</ns2:noOfUnitsOutstanding>
            <ns2:approxSaleDate>08/12/2024</ns2:approxSaleDate>
            <ns2:securitiesExchangeName>NASD</ns2:securitiesExchangeName>
        </ns2:securitiesInformation>
        <ns2:securitiesToBeSold>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:acquiredDate>12/08/2020</ns2:acquiredDate>
            <ns2:natureOfAcquisitionTransaction>Acquired as a gift</ns2:natureOfAcquisitionTransaction>
            <ns2:nameOfPersonfromWhomAcquired>Daniel Bradbury</ns2:nameOfPersonfromWhomAcquired>
            <ns2:isGiftTransaction>Y</ns2:isGiftTransaction>
            <ns2:donarAcquiredDate>01/30/2019</ns2:donarAcquiredDate>
            <ns2:amountOfSecuritiesAcquired>2854</ns2:amountOfSecuritiesAcquired>
            <ns2:paymentDate>12/08/2020</ns2:paymentDate>
            <ns2:natureOfPayment>None</ns2:natureOfPayment>
        </ns2:securitiesToBeSold>
        <ns2:securitiesToBeSold>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:acquiredDate>12/08/2020</ns2:acquiredDate>
            <ns2:natureOfAcquisitionTransaction>Acquired as a gift</ns2:natureOfAcquisitionTransaction>
            <ns2:nameOfPersonfromWhomAcquired>Daniel Bradbury</ns2:nameOfPersonfromWhomAcquired>
            <ns2:isGiftTransaction>Y</ns2:isGiftTransaction>
            <ns2:donarAcquiredDate>01/30/2019</ns2:donarAcquiredDate>
            <ns2:amountOfSecuritiesAcquired>2854</ns2:amountOfSecuritiesAcquired>
            <ns2:paymentDate>12/08/2020</ns2:paymentDate>
            <ns2:natureOfPayment>None</ns2:natureOfPayment>
        </ns2:securitiesToBeSold>
        <ns2:securitiesToBeSold>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:acquiredDate>06/06/2018</ns2:acquiredDate>
            <ns2:natureOfAcquisitionTransaction>Acquired from issuer in private transaction</ns2:natureOfAcquisitionTransaction>
            <ns2:nameOfPersonfromWhomAcquired>Issuer</ns2:nameOfPersonfromWhomAcquired>
            <ns2:isGiftTransaction>N</ns2:isGiftTransaction>
            <ns2:amountOfSecuritiesAcquired>10042</ns2:amountOfSecuritiesAcquired>
            <ns2:paymentDate>06/06/2018</ns2:paymentDate>
            <ns2:natureOfPayment>None</ns2:natureOfPayment>
        </ns2:securitiesToBeSold>
        <ns2:nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</ns2:nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <ns2:remarks>Total number of shares to be sold includes 10,042 shares by BioBrit, LLC, 2,854 shares by The Daniel M. Bradbury Irrevocable Descendants Trust, 2,854  shares by The Annette E. Bradbury Irrevocable Descendants Trust. The sales set forth herein are made in connection with a selling plan dated 5/10/2024, that is intended to comply with Rule 105-1 (c).</ns2:remarks>
        <ns2:noticeSignature>
            <ns2:noticeDate>08/12/2024</ns2:noticeDate>
            <ns2:planAdoptionDates>
                <ns2:planAdoptionDate>05/10/2024</ns2:planAdoptionDate>
            </ns2:planAdoptionDates>
            <ns2:signature>Goldman Sachs &amp; Co. LLC on behalf of Daniel Bradbury</ns2:signature>
        </ns2:noticeSignature>
    </ns2:formData>
</ns2:edgarSubmission>
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144
0001236397
XXXXXXXX
LIVE
0001447362
Castle Biosciences, Inc.
001-38984
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
1-866-788-9007
Daniel Bradbury
Officer
Common
Goldman Sachs & Co. LLC
200 West Street
New York
NY
10282
15750
421312.5
27736760
08/12/2024
NASD
Common
12/08/2020
Acquired as a gift
Daniel Bradbury
Y
01/30/2019
2854
12/08/2020
None
Common
12/08/2020
Acquired as a gift
Daniel Bradbury
Y
01/30/2019
2854
12/08/2020
None
Common
06/06/2018
Acquired from issuer in private transaction
Issuer
N
10042
06/06/2018
None
Y
Total number of shares to be sold includes 10,042 shares by BioBrit, LLC, 2,854 shares by The Daniel M. Bradbury Irrevocable Descendants Trust, 2,854  shares by The Annette E. Bradbury Irrevocable Descendants Trust. The sales set forth herein are made in connection with a selling plan dated 5/10/2024, that is intended to comply with Rule 105-1 (c).
08/12/2024
05/10/2024
Goldman Sachs & Co. LLC on behalf of Daniel Bradbury