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            <liveTestFlag>LIVE</liveTestFlag>



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            <issuerCik>0001717115</issuerCik>
            <issuerName>Tempus AI, Inc. </issuerName>
            <secFileNumber>001-42130</secFileNumber>
            <issuerAddress>
                <ns2:street1>600 WEST CHICAGO AVENUE, Suite 510</ns2:street1>
                <ns2:city>CHICAGO</ns2:city>
                <ns2:stateOrCountry>IL</ns2:stateOrCountry>
                <ns2:zipCode>60654</ns2:zipCode>
            </issuerAddress>
            <issuerContactPhone>8009765448</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>ANDREW POLOVIN</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>Officer</relationshipToIssuer>
            </relationshipsToIssuer>
        </issuerInfo>
        <securitiesInformation>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
                </address>
            </brokerOrMarketmakerDetails>
            <noOfUnitsSold>13135</noOfUnitsSold>
            <aggregateMarketValue>655042.45</aggregateMarketValue>
            <noOfUnitsOutstanding>167168911</noOfUnitsOutstanding>
            <approxSaleDate>03/18/2025</approxSaleDate>
            <securitiesExchangeName>NASDAQ</securitiesExchangeName>
        </securitiesInformation>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>12/15/2024</acquiredDate>
            <natureOfAcquisitionTransaction>RSU/PSU</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>13135</amountOfSecuritiesAcquired>
            <paymentDate>12/15/2024</paymentDate>
            <natureOfPayment>N/A</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
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            <sellerDetails>
                <name>ANDREW POLOVIN</name>
                <address>
                    <ns2:street1>600 WEST CHICAGO AVENUE, Suite 510</ns2:street1>
                    <ns2:city>CHICAGO</ns2:city>
                    <ns2:stateOrCountry>IL</ns2:stateOrCountry>
                    <ns2:zipCode>60654</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>02/19/2025</saleDate>
            <amountOfSecuritiesSold>5551</amountOfSecuritiesSold>
            <grossProceeds>454469.74</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>10b5-1 Sales for ANDREW POLOVIN</name>
                <address>
                    <ns2:street1>600 WEST CHICAGO AVENUE, Suite 510</ns2:street1>
                    <ns2:city>CHICAGO</ns2:city>
                    <ns2:stateOrCountry>IL</ns2:stateOrCountry>
                    <ns2:zipCode>60654</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>02/18/2025</saleDate>
            <amountOfSecuritiesSold>64381</amountOfSecuritiesSold>
            <grossProceeds>5299883.18</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>ANDREW POLOVIN</name>
                <address>
                    <ns2:street1>600 WEST CHICAGO AVENUE, Suite 510</ns2:street1>
                    <ns2:city>CHICAGO</ns2:city>
                    <ns2:stateOrCountry>IL</ns2:stateOrCountry>
                    <ns2:zipCode>60654</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>02/05/2025</saleDate>
            <amountOfSecuritiesSold>7717</amountOfSecuritiesSold>
            <grossProceeds>521437.69</grossProceeds>
        </securitiesSoldInPast3Months>
        <remarks>The securities to be sold were acquired upon the vesting of restricted stock units and performance stock units during the period of 12/15/2024 through 2/1/2025
</remarks>
        <noticeSignature>
            <noticeDate>03/18/2025</noticeDate>
            <planAdoptionDates>
                <planAdoptionDate>09/06/2024</planAdoptionDate>
            </planAdoptionDates>
            <signature>/s/ Andrew Polovin</signature>
        </noticeSignature>
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144
0002024169
XXXXXXXX
LIVE
0001717115
Tempus AI, Inc.
001-42130
600 WEST CHICAGO AVENUE, Suite 510
CHICAGO
IL
60654
8009765448
ANDREW POLOVIN
Officer
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
8th Floor
New York
NY
10004
13135
655042.45
167168911
03/18/2025
NASDAQ
Common
12/15/2024
RSU/PSU
Issuer
N
13135
12/15/2024
N/A
N
ANDREW POLOVIN
600 WEST CHICAGO AVENUE, Suite 510
CHICAGO
IL
60654
Common
02/19/2025
5551
454469.74
10b5-1 Sales for ANDREW POLOVIN
600 WEST CHICAGO AVENUE, Suite 510
CHICAGO
IL
60654
Common
02/18/2025
64381
5299883.18
ANDREW POLOVIN
600 WEST CHICAGO AVENUE, Suite 510
CHICAGO
IL
60654
Common
02/05/2025
7717
521437.69
The securities to be sold were acquired upon the vesting of restricted stock units and performance stock units during the period of 12/15/2024 through 2/1/2025
03/18/2025
09/06/2024
/s/ Andrew Polovin