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            <liveTestFlag>LIVE</liveTestFlag>



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            <secFileNumber>001-38898</secFileNumber>
            <issuerAddress>
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                <ns2:city>New York</ns2:city>
                <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                <ns2:zipCode>10173</ns2:zipCode>
            </issuerAddress>
            <issuerContactPhone>(212) 220-9226</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>ALEXANDRIA VENTURE INVESTMENTS LLC</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>10% Stockholder</relationshipToIssuer>
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            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>38th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
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            </brokerOrMarketmakerDetails>
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            <aggregateMarketValue>329935.00</aggregateMarketValue>
            <noOfUnitsOutstanding>63124227</noOfUnitsOutstanding>
            <approxSaleDate>11/03/2023</approxSaleDate>
            <securitiesExchangeName>NASD</securitiesExchangeName>
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            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>06/23/2022</acquiredDate>
            <natureOfAcquisitionTransaction>Private Acquisition from Issuer/Affiliate</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>143450</amountOfSecuritiesAcquired>
            <paymentDate>06/23/2022</paymentDate>
            <natureOfPayment>Cash</natureOfPayment>
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        <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
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                <address>
                    <ns2:street1>340 Madison Avenue</ns2:street1>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10173</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>11/02/2023</saleDate>
            <amountOfSecuritiesSold>103808</amountOfSecuritiesSold>
            <grossProceeds>241872.64</grossProceeds>
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        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>ALEXANDRIA VENTURE INVESTMENTS LLC</name>
                <address>
                    <ns2:street1>340 Madison Avenue</ns2:street1>
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                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10173</ns2:zipCode>
                </address>
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            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>11/01/2023</saleDate>
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            <grossProceeds>407671.12</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>ALEXANDRIA VENTURE INVESTMENTS LLC</name>
                <address>
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                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10173</ns2:zipCode>
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            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>10/31/2023</saleDate>
            <amountOfSecuritiesSold>155000</amountOfSecuritiesSold>
            <grossProceeds>373550.00</grossProceeds>
        </securitiesSoldInPast3Months>
        <noticeSignature>
            <noticeDate>11/03/2023</noticeDate>
            <planAdoptionDates/>
            <signature>/s/ Marc Binda</signature>
        </noticeSignature>
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144
0001722024
XXXXXXXX
LIVE
0001697532
APPLIED THERAPEUTICS, INC.
001-38898
340 Madison Avenue
New York
NY
10173
(212) 220-9226
ALEXANDRIA VENTURE INVESTMENTS LLC
10% Stockholder
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
38th Floor
New York
NY
10004
143450
329935.00
63124227
11/03/2023
NASD
Common
06/23/2022
Private Acquisition from Issuer/Affiliate
Issuer
N
143450
06/23/2022
Cash
N
ALEXANDRIA VENTURE INVESTMENTS LLC
340 Madison Avenue
New York
NY
10173
Common
11/02/2023
103808
241872.64
ALEXANDRIA VENTURE INVESTMENTS LLC
340 Madison Avenue
New York
NY
10173
Common
11/01/2023
172742
407671.12
ALEXANDRIA VENTURE INVESTMENTS LLC
340 Madison Avenue
New York
NY
10173
Common
10/31/2023
155000
373550.00
11/03/2023
/s/ Marc Binda