<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:ns2="http://www.sec.gov/edgar/common">
    <headerData>
        <submissionType>144</submissionType>
        <filerInfo>
            <filer>
                <filerCredentials>
                    <cik>0001837756</cik>
                    <ccc>XXXXXXXX</ccc>
                </filerCredentials>
            </filer>
            <liveTestFlag>LIVE</liveTestFlag>



        </filerInfo>
    </headerData>
    <formData>
        <issuerInfo>
            <issuerCik>0000834365</issuerCik>
            <issuerName>BIOLIFE SOLUTIONS INC</issuerName>
            <secFileNumber>001-36362</secFileNumber>
            <issuerAddress>
                <ns2:street1>3303 Monte Villa Parkway, Suite 310</ns2:street1>
                <ns2:city>Bothell</ns2:city>
                <ns2:stateOrCountry>WA</ns2:stateOrCountry>
                <ns2:zipCode>98021</ns2:zipCode>
            </issuerAddress>
            <issuerContactPhone>4254021400</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>SARAH AEBERSOLD</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>Officer</relationshipToIssuer>
            </relationshipsToIssuer>
        </issuerInfo>
        <securitiesInformation>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
                </address>
            </brokerOrMarketmakerDetails>
            <noOfUnitsSold>7324</noOfUnitsSold>
            <aggregateMarketValue>194964.88</aggregateMarketValue>
            <noOfUnitsOutstanding>47000000</noOfUnitsOutstanding>
            <approxSaleDate>03/12/2025</approxSaleDate>
            <securitiesExchangeName>NASDAQ</securitiesExchangeName>
        </securitiesInformation>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>03/01/2023</acquiredDate>
            <natureOfAcquisitionTransaction>RSU/RSA/PSU</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>7324</amountOfSecuritiesAcquired>
            <paymentDate>03/01/2023</paymentDate>
            <natureOfPayment>N/A</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>SARAH AEBERSOLD</name>
                <address>
                    <ns2:street1>3303 Monte Villa Parkway, Suite 310</ns2:street1>
                    <ns2:city>Bothell</ns2:city>
                    <ns2:stateOrCountry>WA</ns2:stateOrCountry>
                    <ns2:zipCode>98021</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/03/2025</saleDate>
            <amountOfSecuritiesSold>284</amountOfSecuritiesSold>
            <grossProceeds>7230.64</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>SARAH AEBERSOLD</name>
                <address>
                    <ns2:street1>3303 Monte Villa Parkway, Suite 310</ns2:street1>
                    <ns2:city>Bothell</ns2:city>
                    <ns2:stateOrCountry>WA</ns2:stateOrCountry>
                    <ns2:zipCode>98021</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>02/12/2025</saleDate>
            <amountOfSecuritiesSold>53</amountOfSecuritiesSold>
            <grossProceeds>1406.62</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>SARAH AEBERSOLD</name>
                <address>
                    <ns2:street1>3303 Monte Villa Parkway, Suite 310</ns2:street1>
                    <ns2:city>Bothell</ns2:city>
                    <ns2:stateOrCountry>WA</ns2:stateOrCountry>
                    <ns2:zipCode>98021</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>01/06/2025</saleDate>
            <amountOfSecuritiesSold>240</amountOfSecuritiesSold>
            <grossProceeds>6484.80</grossProceeds>
        </securitiesSoldInPast3Months>
        <remarks>The securities to be sold were acquired upon the vesting of RSUs, RSAs, and PSUs during the period of 03/01/2023 through 10/20/2023.
</remarks>
        <noticeSignature>
            <noticeDate>03/12/2025</noticeDate>
            <planAdoptionDates>
                <planAdoptionDate>12/11/2024</planAdoptionDate>
            </planAdoptionDates>
            <signature>/s/ Sarah Aebersold</signature>
        </noticeSignature>
    </formData>
</edgarSubmission>
[{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 5579}]
144
0001837756
XXXXXXXX
LIVE
0000834365
BIOLIFE SOLUTIONS INC
001-36362
3303 Monte Villa Parkway, Suite 310
Bothell
WA
98021
4254021400
SARAH AEBERSOLD
Officer
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
8th Floor
New York
NY
10004
7324
194964.88
47000000
03/12/2025
NASDAQ
Common
03/01/2023
RSU/RSA/PSU
Issuer
N
7324
03/01/2023
N/A
N
SARAH AEBERSOLD
3303 Monte Villa Parkway, Suite 310
Bothell
WA
98021
Common
03/03/2025
284
7230.64
SARAH AEBERSOLD
3303 Monte Villa Parkway, Suite 310
Bothell
WA
98021
Common
02/12/2025
53
1406.62
SARAH AEBERSOLD
3303 Monte Villa Parkway, Suite 310
Bothell
WA
98021
Common
01/06/2025
240
6484.80
The securities to be sold were acquired upon the vesting of RSUs, RSAs, and PSUs during the period of 03/01/2023 through 10/20/2023.
03/12/2025
12/11/2024
/s/ Sarah Aebersold