<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:ns2="http://www.sec.gov/edgar/common">
    <headerData>
        <submissionType>144</submissionType>
        <filerInfo>
            <filer>
                <filerCredentials>
                    <cik>0001870350</cik>
                    <ccc>XXXXXXXX</ccc>
                </filerCredentials>
            </filer>
            <liveTestFlag>LIVE</liveTestFlag>



        </filerInfo>
    </headerData>
    <formData>
        <issuerInfo>
            <issuerCik>0001562088</issuerCik>
            <issuerName>DUOLINGO, INC.</issuerName>
            <secFileNumber>001-40653</secFileNumber>
            <issuerAddress>
                <ns2:street1>5900 Penn Avenue</ns2:street1>
                <ns2:city>Pittsburgh</ns2:city>
                <ns2:stateOrCountry>PA</ns2:stateOrCountry>
                <ns2:zipCode>15206</ns2:zipCode>
            </issuerAddress>
            <issuerContactPhone>(412) 567-6602</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>NATALIE GLANCE</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>Officer</relationshipToIssuer>
            </relationshipsToIssuer>
        </issuerInfo>
        <securitiesInformation>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
                </address>
            </brokerOrMarketmakerDetails>
            <noOfUnitsSold>2458</noOfUnitsSold>
            <aggregateMarketValue>433157.61</aggregateMarketValue>
            <noOfUnitsOutstanding>36989041</noOfUnitsOutstanding>
            <approxSaleDate>05/15/2024</approxSaleDate>
            <securitiesExchangeName>NASDAQ</securitiesExchangeName>
        </securitiesInformation>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>05/15/2024</acquiredDate>
            <natureOfAcquisitionTransaction>Restricted Stock</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>2458</amountOfSecuritiesAcquired>
            <paymentDate>05/15/2024</paymentDate>
            <natureOfPayment>Not Applicable</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>NATALIE GLANCE</name>
                <address>
                    <ns2:street1>5900 Penn Avenue</ns2:street1>
                    <ns2:city>Pittsburgh</ns2:city>
                    <ns2:stateOrCountry>PA</ns2:stateOrCountry>
                    <ns2:zipCode>15206</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>05/06/2024</saleDate>
            <amountOfSecuritiesSold>1500</amountOfSecuritiesSold>
            <grossProceeds>371734.95</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>NATALIE GLANCE</name>
                <address>
                    <ns2:street1>5900 Penn Avenue</ns2:street1>
                    <ns2:city>Pittsburgh</ns2:city>
                    <ns2:stateOrCountry>PA</ns2:stateOrCountry>
                    <ns2:zipCode>15206</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>04/08/2024</saleDate>
            <amountOfSecuritiesSold>1500</amountOfSecuritiesSold>
            <grossProceeds>323025.00</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>NATALIE GLANCE</name>
                <address>
                    <ns2:street1>5900 Penn Avenue</ns2:street1>
                    <ns2:city>Pittsburgh</ns2:city>
                    <ns2:stateOrCountry>PA</ns2:stateOrCountry>
                    <ns2:zipCode>15206</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/06/2024</saleDate>
            <amountOfSecuritiesSold>1500</amountOfSecuritiesSold>
            <grossProceeds>322065.00</grossProceeds>
        </securitiesSoldInPast3Months>
        <noticeSignature>
            <noticeDate>05/15/2024</noticeDate>
            <planAdoptionDates/>
            <signature>/s/ Natalie Glance</signature>
        </noticeSignature>
    </formData>
</edgarSubmission>
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144
0001870350
XXXXXXXX
LIVE
0001562088
DUOLINGO, INC.
001-40653
5900 Penn Avenue
Pittsburgh
PA
15206
(412) 567-6602
NATALIE GLANCE
Officer
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
8th Floor
New York
NY
10004
2458
433157.61
36989041
05/15/2024
NASDAQ
Common
05/15/2024
Restricted Stock
Issuer
N
2458
05/15/2024
Not Applicable
N
NATALIE GLANCE
5900 Penn Avenue
Pittsburgh
PA
15206
Common
05/06/2024
1500
371734.95
NATALIE GLANCE
5900 Penn Avenue
Pittsburgh
PA
15206
Common
04/08/2024
1500
323025.00
NATALIE GLANCE
5900 Penn Avenue
Pittsburgh
PA
15206
Common
03/06/2024
1500
322065.00
05/15/2024
/s/ Natalie Glance