<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:ns2="http://www.sec.gov/edgar/common">
    <headerData>
        <submissionType>144</submissionType>
        <filerInfo>
            <filer>
                <filerCredentials>
                    <cik>0001439716</cik>
                    <ccc>XXXXXXXX</ccc>
                </filerCredentials>
            </filer>
            <liveTestFlag>LIVE</liveTestFlag>



        </filerInfo>
    </headerData>
    <formData>
        <issuerInfo>
            <issuerCik>0001808805</issuerCik>
            <issuerName>NAUTILUS BIOTECHNOLOGY INC</issuerName>
            <secFileNumber>001-39434</secFileNumber>
            <issuerAddress>
                <ns2:street1>2701 Eastlake Avenue East</ns2:street1>
                <ns2:city>Seattle</ns2:city>
                <ns2:stateOrCountry>WA</ns2:stateOrCountry>
                <ns2:zipCode>98102</ns2:zipCode>
            </issuerAddress>
            <issuerContactPhone>(206) 333-2001</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>MARY GODWIN</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>Officer</relationshipToIssuer>
            </relationshipsToIssuer>
        </issuerInfo>
        <securitiesInformation>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
                </address>
            </brokerOrMarketmakerDetails>
            <noOfUnitsSold>40000</noOfUnitsSold>
            <aggregateMarketValue>98884.00</aggregateMarketValue>
            <noOfUnitsOutstanding>125563965</noOfUnitsOutstanding>
            <approxSaleDate>11/11/2024</approxSaleDate>
            <securitiesExchangeName>NASDAQ</securitiesExchangeName>
        </securitiesInformation>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>11/11/2024</acquiredDate>
            <natureOfAcquisitionTransaction>Stock Option Exercise</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>40000</amountOfSecuritiesAcquired>
            <paymentDate>11/11/2024</paymentDate>
            <natureOfPayment>Cash</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>MARY GODWIN</name>
                <address>
                    <ns2:street1>2701 Eastlake Avenue East</ns2:street1>
                    <ns2:city>Seattle</ns2:city>
                    <ns2:stateOrCountry>WA</ns2:stateOrCountry>
                    <ns2:zipCode>98102</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>11/11/2024</saleDate>
            <amountOfSecuritiesSold>40000</amountOfSecuritiesSold>
            <grossProceeds>98884.00</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>MARY ELIZABETH GODWIN</name>
                <address>
                    <ns2:street1>2701 Eastlake Avenue East</ns2:street1>
                    <ns2:city>Seattle</ns2:city>
                    <ns2:stateOrCountry>WA</ns2:stateOrCountry>
                    <ns2:zipCode>98102</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>11/08/2024</saleDate>
            <amountOfSecuritiesSold>31078</amountOfSecuritiesSold>
            <grossProceeds>78158.06</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>MARY ELIZABETH GODWIN</name>
                <address>
                    <ns2:street1>2701 Eastlake Avenue East</ns2:street1>
                    <ns2:city>Seattle</ns2:city>
                    <ns2:stateOrCountry>WA</ns2:stateOrCountry>
                    <ns2:zipCode>98102</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>11/07/2024</saleDate>
            <amountOfSecuritiesSold>28922</amountOfSecuritiesSold>
            <grossProceeds>79642.51</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>MARY ELIZABETH GODWIN</name>
                <address>
                    <ns2:street1>2701 Eastlake Avenue East</ns2:street1>
                    <ns2:city>Seattle</ns2:city>
                    <ns2:stateOrCountry>WA</ns2:stateOrCountry>
                    <ns2:zipCode>98102</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>09/23/2024</saleDate>
            <amountOfSecuritiesSold>47031</amountOfSecuritiesSold>
            <grossProceeds>134739.11</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>MARY ELIZABETH GODWIN</name>
                <address>
                    <ns2:street1>2701 Eastlake Avenue East</ns2:street1>
                    <ns2:city>Seattle</ns2:city>
                    <ns2:stateOrCountry>WA</ns2:stateOrCountry>
                    <ns2:zipCode>98102</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>09/17/2024</saleDate>
            <amountOfSecuritiesSold>35000</amountOfSecuritiesSold>
            <grossProceeds>99865.50</grossProceeds>
        </securitiesSoldInPast3Months>
        <remarks>Sales that were executed on November 11, 2024 are being reported on this Form 144 filed on November 12, 2024 given the EDGAR system was not accepting filings November 11, 2024 in observance of the federal holiday. </remarks>
        <noticeSignature>
            <noticeDate>11/12/2024</noticeDate>
            <planAdoptionDates/>
            <signature>/s/ Mary Elizabeth Godwin</signature>
        </noticeSignature>
    </formData>
</edgarSubmission>
[{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 6975}]
144
0001439716
XXXXXXXX
LIVE
0001808805
NAUTILUS BIOTECHNOLOGY INC
001-39434
2701 Eastlake Avenue East
Seattle
WA
98102
(206) 333-2001
MARY GODWIN
Officer
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
8th Floor
New York
NY
10004
40000
98884.00
125563965
11/11/2024
NASDAQ
Common
11/11/2024
Stock Option Exercise
Issuer
N
40000
11/11/2024
Cash
N
MARY GODWIN
2701 Eastlake Avenue East
Seattle
WA
98102
Common
11/11/2024
40000
98884.00
MARY ELIZABETH GODWIN
2701 Eastlake Avenue East
Seattle
WA
98102
Common
11/08/2024
31078
78158.06
MARY ELIZABETH GODWIN
2701 Eastlake Avenue East
Seattle
WA
98102
Common
11/07/2024
28922
79642.51
MARY ELIZABETH GODWIN
2701 Eastlake Avenue East
Seattle
WA
98102
Common
09/23/2024
47031
134739.11
MARY ELIZABETH GODWIN
2701 Eastlake Avenue East
Seattle
WA
98102
Common
09/17/2024
35000
99865.50
Sales that were executed on November 11, 2024 are being reported on this Form 144 filed on November 12, 2024 given the EDGAR system was not accepting filings November 11, 2024 in observance of the federal holiday.
11/12/2024
/s/ Mary Elizabeth Godwin