<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
  <headerData>
    <submissionType>144</submissionType>
    <filerInfo>
      <filer>
        <filerCredentials>
          <cik>0001965469</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>



    </filerInfo>
  </headerData>
  <formData>
    <issuerInfo>
      <issuerCik>0001447669</issuerCik>
      <issuerName>TWILIO INC</issuerName>
      <secFileNumber>001-37806</secFileNumber>
      <issuerAddress>
        <com:street1>101 SPEAR STREET</com:street1>
        <com:street2>SUITE 500</com:street2>
        <com:city>San Francisco</com:city>
        <com:stateOrCountry>CA</com:stateOrCountry>
        <com:zipCode>94105</com:zipCode>
      </issuerAddress>
      <issuerContactPhone>415-390-2337</issuerContactPhone>
      <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Viggiano Aidan</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <relationshipsToIssuer>
        <relationshipToIssuer>Officer</relationshipToIssuer>
      </relationshipsToIssuer>
    </issuerInfo>
    <securitiesInformation>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <brokerOrMarketmakerDetails>
        <name>Charles Schwab &amp; Co., Inc.</name>
        <address>
          <com:street1>3000 Schwab Way</com:street1>
          <com:city>Westlake</com:city>
          <com:stateOrCountry>TX</com:stateOrCountry>
          <com:zipCode>76262</com:zipCode>
        </address>
      </brokerOrMarketmakerDetails>
      <noOfUnitsSold>7524</noOfUnitsSold>
      <aggregateMarketValue>721384.00</aggregateMarketValue>
      <noOfUnitsOutstanding>152633605</noOfUnitsOutstanding>
      <approxSaleDate>03/31/2025</approxSaleDate>
      <securitiesExchangeName>NYSE</securitiesExchangeName>
    </securitiesInformation>
    <securitiesToBeSold>
      <securitiesClassTitle>Common</securitiesClassTitle>
      <acquiredDate>03/31/2025</acquiredDate>
      <natureOfAcquisitionTransaction>Restricted Stock Lapse - See Remarks</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>TWILIO INC.</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>7524</amountOfSecuritiesAcquired>
      <paymentDate>03/31/2025</paymentDate>
      <natureOfPayment>Equity Compensation</natureOfPayment>
    </securitiesToBeSold>
    <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>AIDAN VIGGIANO</name>
        <address>
          <com:street1>101 Spear Street, Suite 500</com:street1>
          <com:city>San Francisco</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94105</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>TWILIO INC</securitiesClassTitle>
      <saleDate>12/31/2024</saleDate>
      <amountOfSecuritiesSold>6832</amountOfSecuritiesSold>
      <grossProceeds>732136.00</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>AIDAN VIGGIANO</name>
        <address>
          <com:street1>101 Spear Street, Suite 500</com:street1>
          <com:city>San Francisco</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94105</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>TWILIO INC</securitiesClassTitle>
      <saleDate>01/06/2025</saleDate>
      <amountOfSecuritiesSold>6552</amountOfSecuritiesSold>
      <grossProceeds>743963.00</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>AIDAN VIGGIANO</name>
        <address>
          <com:street1>101 Spear Street, Suite 500</com:street1>
          <com:city>San Francisco</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94105</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>TWILIO INC</securitiesClassTitle>
      <saleDate>02/18/2025</saleDate>
      <amountOfSecuritiesSold>1391</amountOfSecuritiesSold>
      <grossProceeds>173198.00</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>AIDAN VIGGIANO</name>
        <address>
          <com:street1>101 Spear Street, Suite 500</com:street1>
          <com:city>San Francisco</com:city>
          <com:stateOrCountry>CA</com:stateOrCountry>
          <com:zipCode>94105</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>TWILIO INC</securitiesClassTitle>
      <saleDate>02/21/2025</saleDate>
      <amountOfSecuritiesSold>2500</amountOfSecuritiesSold>
      <grossProceeds>293625.00</grossProceeds>
    </securitiesSoldInPast3Months>
    <remarks>Shares sold to cover tax obligation from settlement of vested Restricted Stock Units.</remarks>
    <noticeSignature>
      <noticeDate>03/31/2025</noticeDate>
      <signature>AIDAN VIGGIANO</signature>
    </noticeSignature>
  </formData>
</edgarSubmission>
[{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 5286}]
144
0001965469
XXXXXXXX
LIVE
0001447669
TWILIO INC
001-37806
101 SPEAR STREET
SUITE 500
San Francisco
CA
94105
415-390-2337
Viggiano Aidan
Officer
Common
Charles Schwab & Co., Inc.
3000 Schwab Way
Westlake
TX
76262
7524
721384.00
152633605
03/31/2025
NYSE
Common
03/31/2025
Restricted Stock Lapse - See Remarks
TWILIO INC.
N
7524
03/31/2025
Equity Compensation
N
AIDAN VIGGIANO
101 Spear Street, Suite 500
San Francisco
CA
94105
TWILIO INC
12/31/2024
6832
732136.00
AIDAN VIGGIANO
101 Spear Street, Suite 500
San Francisco
CA
94105
TWILIO INC
01/06/2025
6552
743963.00
AIDAN VIGGIANO
101 Spear Street, Suite 500
San Francisco
CA
94105
TWILIO INC
02/18/2025
1391
173198.00
AIDAN VIGGIANO
101 Spear Street, Suite 500
San Francisco
CA
94105
TWILIO INC
02/21/2025
2500
293625.00
Shares sold to cover tax obligation from settlement of vested Restricted Stock Units.
03/31/2025
AIDAN VIGGIANO