<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:ns2="http://www.sec.gov/edgar/common">
    <headerData>
        <submissionType>144</submissionType>
        <filerInfo>
            <filer>
                <filerCredentials>
                    <cik>0001585799</cik>
                    <ccc>XXXXXXXX</ccc>
                </filerCredentials>
            </filer>
            <liveTestFlag>LIVE</liveTestFlag>



        </filerInfo>
    </headerData>
    <formData>
        <issuerInfo>
            <issuerCik>0001660280</issuerCik>
            <issuerName>TENABLE HOLDINGS, INC.</issuerName>
            <secFileNumber>001-38600</secFileNumber>
            <issuerAddress>
                <ns2:street1>6100 Merriweather Drive</ns2:street1>
                <ns2:city>Columbia</ns2:city>
                <ns2:stateOrCountry>MD</ns2:stateOrCountry>
                <ns2:zipCode>21044</ns2:zipCode>
            </issuerAddress>
            <issuerContactPhone>(410) 872-0555</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>LINDA K ZECHER</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>Director</relationshipToIssuer>
            </relationshipsToIssuer>
        </issuerInfo>
        <securitiesInformation>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>38th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
                </address>
            </brokerOrMarketmakerDetails>
            <noOfUnitsSold>3320</noOfUnitsSold>
            <aggregateMarketValue>121155.76</aggregateMarketValue>
            <noOfUnitsOutstanding>113648357</noOfUnitsOutstanding>
            <approxSaleDate>04/27/2023</approxSaleDate>
            <securitiesExchangeName>NASDAQ</securitiesExchangeName>
        </securitiesInformation>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>05/25/2022</acquiredDate>
            <natureOfAcquisitionTransaction>Restricted Stock Unit Vested</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>144</amountOfSecuritiesAcquired>
            <paymentDate>05/25/2022</paymentDate>
            <natureOfPayment>Not Applicable</natureOfPayment>
        </securitiesToBeSold>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>08/08/2022</acquiredDate>
            <natureOfAcquisitionTransaction>Restricted Stock Unit Vested</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>3176</amountOfSecuritiesAcquired>
            <paymentDate>08/08/2022</paymentDate>
            <natureOfPayment>Not Applicable</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>LINDA K ZECHER</name>
                <address>
                    <ns2:street1>6100 Merriweather Drive</ns2:street1>
                    <ns2:city>Columbia</ns2:city>
                    <ns2:stateOrCountry>MD</ns2:stateOrCountry>
                    <ns2:zipCode>21044</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>02/10/2023</saleDate>
            <amountOfSecuritiesSold>7000</amountOfSecuritiesSold>
            <grossProceeds>309698.20</grossProceeds>
        </securitiesSoldInPast3Months>
        <noticeSignature>
            <noticeDate>04/27/2023</noticeDate>
            <planAdoptionDates/>
            <signature>/s/ Linda K. Zecher</signature>
        </noticeSignature>
    </formData>
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144
0001585799
XXXXXXXX
LIVE
0001660280
TENABLE HOLDINGS, INC.
001-38600
6100 Merriweather Drive
Columbia
MD
21044
(410) 872-0555
LINDA K ZECHER
Director
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
38th Floor
New York
NY
10004
3320
121155.76
113648357
04/27/2023
NASDAQ
Common
05/25/2022
Restricted Stock Unit Vested
Issuer
N
144
05/25/2022
Not Applicable
Common
08/08/2022
Restricted Stock Unit Vested
Issuer
N
3176
08/08/2022
Not Applicable
N
LINDA K ZECHER
6100 Merriweather Drive
Columbia
MD
21044
Common
02/10/2023
7000
309698.20
04/27/2023
/s/ Linda K. Zecher