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            <ns2:filer>
                <ns2:filerCredentials>
                    <ns2:cik>0001236397</ns2:cik>
                    <ns2:ccc>XXXXXXXX</ns2:ccc>
                </ns2:filerCredentials>
            </ns2:filer>
            <ns2:liveTestFlag>LIVE</ns2:liveTestFlag>



        </ns2:filerInfo>
    </ns2:headerData>
    <ns2:formData>
        <ns2:issuerInfo>
            <ns2:issuerCik>0001447362</ns2:issuerCik>
            <ns2:issuerName>Castle Biosciences, Inc.</ns2:issuerName>
            <ns2:secFileNumber>001-38984</ns2:secFileNumber>
            <ns2:issuerAddress>
                <com:street1>505 S. Friendswood Drive</com:street1>
                <com:street2>Suite 401</com:street2>
                <com:city>Friendswood</com:city>
                <com:stateOrCountry>TX</com:stateOrCountry>
                <com:zipCode>77546</com:zipCode>
            </ns2:issuerAddress>
            <ns2:issuerContactPhone>1-866-788-9007</ns2:issuerContactPhone>
            <ns2:nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Daniel Bradbury</ns2:nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <ns2:relationshipsToIssuer>
                <ns2:relationshipToIssuer>Officer</ns2:relationshipToIssuer>
            </ns2:relationshipsToIssuer>
        </ns2:issuerInfo>
        <ns2:securitiesInformation>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:brokerOrMarketmakerDetails>
                <ns2:name>Goldman Sachs &amp; Co. LLC</ns2:name>
                <ns2:address>
                    <com:street1>200 West Street</com:street1>
                    <com:city>New York</com:city>
                    <com:stateOrCountry>NY</com:stateOrCountry>
                    <com:zipCode>10282</com:zipCode>
                </ns2:address>
            </ns2:brokerOrMarketmakerDetails>
            <ns2:noOfUnitsSold>7867</ns2:noOfUnitsSold>
            <ns2:aggregateMarketValue>221220.04</ns2:aggregateMarketValue>
            <ns2:noOfUnitsOutstanding>28007301</ns2:noOfUnitsOutstanding>
            <ns2:approxSaleDate>02/05/2025</ns2:approxSaleDate>
            <ns2:securitiesExchangeName>NASD</ns2:securitiesExchangeName>
        </ns2:securitiesInformation>
        <ns2:securitiesToBeSold>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:acquiredDate>12/08/2020</ns2:acquiredDate>
            <ns2:natureOfAcquisitionTransaction>Acquired as a gift</ns2:natureOfAcquisitionTransaction>
            <ns2:nameOfPersonfromWhomAcquired>Daniel M. Bradbury</ns2:nameOfPersonfromWhomAcquired>
            <ns2:isGiftTransaction>Y</ns2:isGiftTransaction>
            <ns2:donarAcquiredDate>09/12/2008</ns2:donarAcquiredDate>
            <ns2:amountOfSecuritiesAcquired>1425</ns2:amountOfSecuritiesAcquired>
            <ns2:paymentDate>12/08/2020</ns2:paymentDate>
            <ns2:natureOfPayment>N/A</ns2:natureOfPayment>
        </ns2:securitiesToBeSold>
        <ns2:securitiesToBeSold>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:acquiredDate>12/08/2020</ns2:acquiredDate>
            <ns2:natureOfAcquisitionTransaction>Acquired as a gift</ns2:natureOfAcquisitionTransaction>
            <ns2:nameOfPersonfromWhomAcquired>Daniel M. Bradbury</ns2:nameOfPersonfromWhomAcquired>
            <ns2:isGiftTransaction>Y</ns2:isGiftTransaction>
            <ns2:donarAcquiredDate>09/12/2008</ns2:donarAcquiredDate>
            <ns2:amountOfSecuritiesAcquired>1425</ns2:amountOfSecuritiesAcquired>
            <ns2:paymentDate>12/08/2020</ns2:paymentDate>
            <ns2:natureOfPayment>N/A</ns2:natureOfPayment>
        </ns2:securitiesToBeSold>
        <ns2:securitiesToBeSold>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:acquiredDate>06/08/2018</ns2:acquiredDate>
            <ns2:natureOfAcquisitionTransaction>Acquired from issuer in private transaction</ns2:natureOfAcquisitionTransaction>
            <ns2:nameOfPersonfromWhomAcquired>Issuer</ns2:nameOfPersonfromWhomAcquired>
            <ns2:isGiftTransaction>N</ns2:isGiftTransaction>
            <ns2:amountOfSecuritiesAcquired>5017</ns2:amountOfSecuritiesAcquired>
            <ns2:paymentDate>06/08/2018</ns2:paymentDate>
            <ns2:natureOfPayment>N/A</ns2:natureOfPayment>
        </ns2:securitiesToBeSold>
        <ns2:nothingToReportFlagOnSecuritiesSoldInPast3Months>N</ns2:nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>The Daniel M. Bradbury Irrevocable Descendants Trust</ns2:name>
                <ns2:address>
                    <com:street1>505 S. Friendswood Drive</com:street1>
                    <com:street2>Suite 401</com:street2>
                    <com:city>Friendswood</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>01/06/2025</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>1425</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>41524.36</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>The Annette E. Bradbury Irrevocable Descendants Trust</ns2:name>
                <ns2:address>
                    <com:street1>505 S. Friendswood Drive</com:street1>
                    <com:street2>Suite 401</com:street2>
                    <com:city>Friendswood</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>01/06/2025</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>1425</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>41524.36</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>BioBrit, LLC</ns2:name>
                <ns2:address>
                    <com:street1>505 S. Friendswood Drive</com:street1>
                    <com:street2>Suite 401</com:street2>
                    <com:city>Friendswood</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>01/06/2025</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>5017</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>146194.88</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>The Daniel M. Bradbury Irrevocable Descendants Trust</ns2:name>
                <ns2:address>
                    <com:street1>505 S. Friendswood Drive</com:street1>
                    <com:street2>Suite 401</com:street2>
                    <com:city>Friendswood</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>12/03/2024</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>1425</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>44142.08</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>The Annette E. Bradbury Irrevocable Descendants Trust</ns2:name>
                <ns2:address>
                    <com:street1>505 S. Friendswood Drive</com:street1>
                    <com:street2>Suite 401</com:street2>
                    <com:city>Friendswood</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>12/03/2024</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>1425</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>44142.08</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>BioBrit, LLC</ns2:name>
                <ns2:address>
                    <com:street1>505 S. Friendswood Drive</com:street1>
                    <com:street2>Suite 401</com:street2>
                    <com:city>Friendswood</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>12/03/2024</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>5017</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>155411.11</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:remarks>Total number of shares to be sold includes 5,017 shares by BioBrit, LLC, 1,425 shares by The Daniel M. Bradbury Irrevocable Descendants Trust, 1,425 shares by The Annette E. Bradbury Irrevocable Descendants Trust. The sales set forth herein are made in connection with a selling plan dated 5/10/2024, that is intended to comply with Rule 105-1 (c).</ns2:remarks>
        <ns2:noticeSignature>
            <ns2:noticeDate>02/05/2025</ns2:noticeDate>
            <ns2:planAdoptionDates>
                <ns2:planAdoptionDate>05/10/2024</ns2:planAdoptionDate>
            </ns2:planAdoptionDates>
            <ns2:signature>Goldman Sachs &amp; Co. LLC on behalf of Daniel M. Bradbury</ns2:signature>
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144
0001236397
XXXXXXXX
LIVE
0001447362
Castle Biosciences, Inc.
001-38984
505 S. Friendswood Drive
Suite 401
Friendswood
TX
77546
1-866-788-9007
Daniel Bradbury
Officer
Common
Goldman Sachs & Co. LLC
200 West Street
New York
NY
10282
7867
221220.04
28007301
02/05/2025
NASD
Common
12/08/2020
Acquired as a gift
Daniel M. Bradbury
Y
09/12/2008
1425
12/08/2020
N/A
Common
12/08/2020
Acquired as a gift
Daniel M. Bradbury
Y
09/12/2008
1425
12/08/2020
N/A
Common
06/08/2018
Acquired from issuer in private transaction
Issuer
N
5017
06/08/2018
N/A
N
The Daniel M. Bradbury Irrevocable Descendants Trust
505 S. Friendswood Drive
Suite 401
Friendswood
TX
77546
Common
01/06/2025
1425
41524.36
The Annette E. Bradbury Irrevocable Descendants Trust
505 S. Friendswood Drive
Suite 401
Friendswood
TX
77546
Common
01/06/2025
1425
41524.36
BioBrit, LLC
505 S. Friendswood Drive
Suite 401
Friendswood
TX
77546
Common
01/06/2025
5017
146194.88
The Daniel M. Bradbury Irrevocable Descendants Trust
505 S. Friendswood Drive
Suite 401
Friendswood
TX
77546
Common
12/03/2024
1425
44142.08
The Annette E. Bradbury Irrevocable Descendants Trust
505 S. Friendswood Drive
Suite 401
Friendswood
TX
77546
Common
12/03/2024
1425
44142.08
BioBrit, LLC
505 S. Friendswood Drive
Suite 401
Friendswood
TX
77546
Common
12/03/2024
5017
155411.11
Total number of shares to be sold includes 5,017 shares by BioBrit, LLC, 1,425 shares by The Daniel M. Bradbury Irrevocable Descendants Trust, 1,425 shares by The Annette E. Bradbury Irrevocable Descendants Trust. The sales set forth herein are made in connection with a selling plan dated 5/10/2024, that is intended to comply with Rule 105-1 (c).
02/05/2025
05/10/2024
Goldman Sachs & Co. LLC on behalf of Daniel M. Bradbury