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            <issuerName>MIDWESTONE FINANCIAL GROUP, INC.</issuerName>
            <secFileNumber>001-35968</secFileNumber>
            <issuerAddress>
                <ns2:street1>102 South Clinton Street</ns2:street1>
                <ns2:city>Iowa City</ns2:city>
                <ns2:stateOrCountry>IA</ns2:stateOrCountry>
                <ns2:zipCode>52240</ns2:zipCode>
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            <issuerContactPhone>319-356-5800</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>CAROLINE E FUNK REV TRUST</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
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            <brokerOrMarketmakerDetails>
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                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
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            <aggregateMarketValue>20400.00</aggregateMarketValue>
            <noOfUnitsOutstanding>15755319</noOfUnitsOutstanding>
            <approxSaleDate>06/10/2024</approxSaleDate>
            <securitiesExchangeName>NASDAQ</securitiesExchangeName>
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            <natureOfAcquisitionTransaction>Open Market Purchase</natureOfAcquisitionTransaction>
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            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>600</amountOfSecuritiesAcquired>
            <paymentDate>12/08/2008</paymentDate>
            <natureOfPayment>Cash</natureOfPayment>
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        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
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            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>400</amountOfSecuritiesAcquired>
            <paymentDate>07/02/2009</paymentDate>
            <natureOfPayment>Cash</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
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                    <ns2:zipCode>52240</ns2:zipCode>
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            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>06/06/2024</saleDate>
            <amountOfSecuritiesSold>8</amountOfSecuritiesSold>
            <grossProceeds>166.80</grossProceeds>
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        <securitiesSoldInPast3Months>
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                    <ns2:stateOrCountry>IA</ns2:stateOrCountry>
                    <ns2:zipCode>52240</ns2:zipCode>
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            <securitiesClassTitle>Common</securitiesClassTitle>
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            <amountOfSecuritiesSold>1000</amountOfSecuritiesSold>
            <grossProceeds>20850.00</grossProceeds>
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                    <ns2:stateOrCountry>IA</ns2:stateOrCountry>
                    <ns2:zipCode>52240</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>05/21/2024</saleDate>
            <amountOfSecuritiesSold>8</amountOfSecuritiesSold>
            <grossProceeds>176.96</grossProceeds>
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        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>CAROLINE E FUNK REV TRUST</name>
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                    <ns2:stateOrCountry>IA</ns2:stateOrCountry>
                    <ns2:zipCode>52240</ns2:zipCode>
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            <securitiesClassTitle>Common</securitiesClassTitle>
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                    <ns2:zipCode>52240</ns2:zipCode>
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            <securitiesClassTitle>Common</securitiesClassTitle>
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            <grossProceeds>12854.25</grossProceeds>
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                    <ns2:stateOrCountry>IA</ns2:stateOrCountry>
                    <ns2:zipCode>52240</ns2:zipCode>
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            <securitiesClassTitle>Common</securitiesClassTitle>
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            <amountOfSecuritiesSold>5000</amountOfSecuritiesSold>
            <grossProceeds>107675.00</grossProceeds>
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            <grossProceeds>35443.23</grossProceeds>
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        <securitiesSoldInPast3Months>
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        <securitiesSoldInPast3Months>
            <sellerDetails>
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        <noticeSignature>
            <noticeDate>06/10/2024</noticeDate>
            <planAdoptionDates/>
            <signature>/s/ Charles N Funk</signature>
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144
0001430014
XXXXXXXX
LIVE
0001412665
MIDWESTONE FINANCIAL GROUP, INC.
001-35968
102 South Clinton Street
Iowa City
IA
52240
319-356-5800
CAROLINE E FUNK REV TRUST
Director
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
8th Floor
New York
NY
10004
1000
20400.00
15755319
06/10/2024
NASDAQ
Common
12/08/2008
Open Market Purchase
Open Market
N
600
12/08/2008
Cash
Common
07/02/2009
Open Market Purchase
Open Market
N
400
07/02/2009
Cash
N
CAROLINE E FUNK REV TRUST
102 South Clinton Street
Iowa City
IA
52240
Common
06/06/2024
8
166.80
CAROLINE E FUNK REV TRUST
102 South Clinton Street
Iowa City
IA
52240
Common
06/05/2024
1000
20850.00
CAROLINE E FUNK REV TRUST
102 South Clinton Street
Iowa City
IA
52240
Common
05/21/2024
8
176.96
CAROLINE E FUNK REV TRUST
102 South Clinton Street
Iowa City
IA
52240
Common
05/10/2024
1000
21999.20
CAROLINE E FUNK REV TRUST
102 South Clinton Street
Iowa City
IA
52240
Common
05/10/2024
591
12854.25
CAROLINE E FUNK REV TRUST
102 South Clinton Street
Iowa City
IA
52240
Common
05/09/2024
5000
107675.00
CAROLINE E FUNK REV TRUST
102 South Clinton Street
Iowa City
IA
52240
Common
05/07/2024
1657
35443.23
CAROLINE E FUNK REV TRUST
102 South Clinton Street
Iowa City
IA
52240
Common
05/07/2024
1000
21170.00
CAROLINE E FUNK REV TRUST
102 South Clinton Street
Iowa City
IA
52240
Common
05/06/2024
2000
42700.00
CAROLINE E FUNK REV TRUST
102 South Clinton Street
Iowa City
IA
52240
Common
05/06/2024
1000
21500.00
CAROLINE E FUNK REV TRUST
102 South Clinton Street
Iowa City
IA
52240
Common
05/03/2024
343
7340.20
06/10/2024
/s/ Charles N Funk