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        <submissionType>144</submissionType>
        <filerInfo>
            <filer>
                <filerCredentials>
                    <cik>0001841671</cik>
                    <ccc>XXXXXXXX</ccc>
                </filerCredentials>
            </filer>
            <liveTestFlag>LIVE</liveTestFlag>



        </filerInfo>
    </headerData>
    <formData>
        <issuerInfo>
            <issuerCik>0001110611</issuerCik>
            <issuerName>ON24, INC.</issuerName>
            <secFileNumber>001-39965</secFileNumber>
            <issuerAddress>
                <ns2:street1>50 Beale Street</ns2:street1>
                <ns2:street2>8th Floor</ns2:street2>
                <ns2:city>San Francisco</ns2:city>
                <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                <ns2:zipCode>94105</ns2:zipCode>
            </issuerAddress>
            <issuerContactPhone>(415) 369-8000</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>JAMES BLACKIE</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>Officer</relationshipToIssuer>
            </relationshipsToIssuer>
        </issuerInfo>
        <securitiesInformation>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
                </address>
            </brokerOrMarketmakerDetails>
            <noOfUnitsSold>16767</noOfUnitsSold>
            <aggregateMarketValue>92835.53</aggregateMarketValue>
            <noOfUnitsOutstanding>41650776</noOfUnitsOutstanding>
            <approxSaleDate>03/10/2025</approxSaleDate>
            <securitiesExchangeName>NYSE</securitiesExchangeName>
        </securitiesInformation>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>08/20/2023</acquiredDate>
            <natureOfAcquisitionTransaction>Restricted stock vesting under a registered plan</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>16767</amountOfSecuritiesAcquired>
            <paymentDate>08/20/2023</paymentDate>
            <natureOfPayment>Not Applicable</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>JAMES BLACKIE</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>San Francisco</ns2:city>
                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/07/2025</saleDate>
            <amountOfSecuritiesSold>7433</amountOfSecuritiesSold>
            <grossProceeds>43258.57</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>JAMES BLACKIE</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>San Francisco</ns2:city>
                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/06/2025</saleDate>
            <amountOfSecuritiesSold>800</amountOfSecuritiesSold>
            <grossProceeds>4644.48</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>James Blackie</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>San Francisco</ns2:city>
                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>common</securitiesClassTitle>
            <saleDate>03/04/2025</saleDate>
            <amountOfSecuritiesSold>5616</amountOfSecuritiesSold>
            <grossProceeds>31191.83</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>James Blackie</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>San Francisco</ns2:city>
                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>common</securitiesClassTitle>
            <saleDate>03/04/2025</saleDate>
            <amountOfSecuritiesSold>5530</amountOfSecuritiesSold>
            <grossProceeds>30714.17</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>JAMES BLACKIE</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
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                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/04/2025</saleDate>
            <amountOfSecuritiesSold>4219</amountOfSecuritiesSold>
            <grossProceeds>23315.46</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>JAMES BLACKIE</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
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                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>03/03/2025</saleDate>
            <amountOfSecuritiesSold>6927</amountOfSecuritiesSold>
            <grossProceeds>38590.32</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>James Blackie</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
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                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>common</securitiesClassTitle>
            <saleDate>02/20/2025</saleDate>
            <amountOfSecuritiesSold>3609</amountOfSecuritiesSold>
            <grossProceeds>23014.99</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>JAMES BLACKIE</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
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                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>02/20/2025</saleDate>
            <amountOfSecuritiesSold>3609</amountOfSecuritiesSold>
            <grossProceeds>23014.95</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>James Blackie</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>San Francisco</ns2:city>
                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>common</securitiesClassTitle>
            <saleDate>02/11/2025</saleDate>
            <amountOfSecuritiesSold>3177</amountOfSecuritiesSold>
            <grossProceeds>21518.14</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>JAMES BLACKIE</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>San Francisco</ns2:city>
                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>02/11/2025</saleDate>
            <amountOfSecuritiesSold>1605</amountOfSecuritiesSold>
            <grossProceeds>10745.80</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>JAMES BLACKIE</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>San Francisco</ns2:city>
                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>02/10/2025</saleDate>
            <amountOfSecuritiesSold>1572</amountOfSecuritiesSold>
            <grossProceeds>10772.29</grossProceeds>
        </securitiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>JAMES BLACKIE</name>
                <address>
                    <ns2:street1>50 Beale Street</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>San Francisco</ns2:city>
                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94105</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>12/16/2024</saleDate>
            <amountOfSecuritiesSold>8000</amountOfSecuritiesSold>
            <grossProceeds>55689.60</grossProceeds>
        </securitiesSoldInPast3Months>
        <noticeSignature>
            <noticeDate>03/10/2025</noticeDate>
            <planAdoptionDates/>
            <signature>/s/ James Blackie</signature>
        </noticeSignature>
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144
0001841671
XXXXXXXX
LIVE
0001110611
ON24, INC.
001-39965
50 Beale Street
8th Floor
San Francisco
CA
94105
(415) 369-8000
JAMES BLACKIE
Officer
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
8th Floor
New York
NY
10004
16767
92835.53
41650776
03/10/2025
NYSE
Common
08/20/2023
Restricted stock vesting under a registered plan
Issuer
N
16767
08/20/2023
Not Applicable
N
JAMES BLACKIE
50 Beale Street
8th Floor
San Francisco
CA
94105
Common
03/07/2025
7433
43258.57
JAMES BLACKIE
50 Beale Street
8th Floor
San Francisco
CA
94105
Common
03/06/2025
800
4644.48
James Blackie
50 Beale Street
8th Floor
San Francisco
CA
94105
common
03/04/2025
5616
31191.83
James Blackie
50 Beale Street
8th Floor
San Francisco
CA
94105
common
03/04/2025
5530
30714.17
JAMES BLACKIE
50 Beale Street
8th Floor
San Francisco
CA
94105
Common
03/04/2025
4219
23315.46
JAMES BLACKIE
50 Beale Street
8th Floor
San Francisco
CA
94105
Common
03/03/2025
6927
38590.32
James Blackie
50 Beale Street
8th Floor
San Francisco
CA
94105
common
02/20/2025
3609
23014.99
JAMES BLACKIE
50 Beale Street
8th Floor
San Francisco
CA
94105
Common
02/20/2025
3609
23014.95
James Blackie
50 Beale Street
8th Floor
San Francisco
CA
94105
common
02/11/2025
3177
21518.14
JAMES BLACKIE
50 Beale Street
8th Floor
San Francisco
CA
94105
Common
02/11/2025
1605
10745.80
JAMES BLACKIE
50 Beale Street
8th Floor
San Francisco
CA
94105
Common
02/10/2025
1572
10772.29
JAMES BLACKIE
50 Beale Street
8th Floor
San Francisco
CA
94105
Common
12/16/2024
8000
55689.60
03/10/2025
/s/ James Blackie