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    <headerData>
        <submissionType>144</submissionType>
        <filerInfo>
            <filer>
                <filerCredentials>
                    <cik>0001430014</cik>
                    <ccc>XXXXXXXX</ccc>
                </filerCredentials>
            </filer>
            <liveTestFlag>LIVE</liveTestFlag>



        </filerInfo>
    </headerData>
    <formData>
        <issuerInfo>
            <issuerCik>0001412665</issuerCik>
            <issuerName>MIDWESTONE FINANCIAL GROUP, INC.</issuerName>
            <secFileNumber>001-35968</secFileNumber>
            <issuerAddress>
                <ns2:street1>102 South Clinton Street</ns2:street1>
                <ns2:city>Iowa City</ns2:city>
                <ns2:stateOrCountry>IA</ns2:stateOrCountry>
                <ns2:zipCode>52240</ns2:zipCode>
            </issuerAddress>
            <issuerContactPhone>319-356-5800</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>CAROLINE E FUNK REV TRUST</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>Director</relationshipToIssuer>
            </relationshipsToIssuer>
        </issuerInfo>
        <securitiesInformation>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
                </address>
            </brokerOrMarketmakerDetails>
            <noOfUnitsSold>3000</noOfUnitsSold>
            <aggregateMarketValue>64200.00</aggregateMarketValue>
            <noOfUnitsOutstanding>15750471</noOfUnitsOutstanding>
            <approxSaleDate>05/06/2024</approxSaleDate>
            <securitiesExchangeName>NASDAQ</securitiesExchangeName>
        </securitiesInformation>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>03/19/2020</acquiredDate>
            <natureOfAcquisitionTransaction>Open Market Purchase</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Open Market</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>1000</amountOfSecuritiesAcquired>
            <paymentDate>03/19/2020</paymentDate>
            <natureOfPayment>Cash</natureOfPayment>
        </securitiesToBeSold>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>04/10/2008</acquiredDate>
            <natureOfAcquisitionTransaction>Open Market Purchase</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Open Market</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>1000</amountOfSecuritiesAcquired>
            <paymentDate>04/10/2008</paymentDate>
            <natureOfPayment>Cash</natureOfPayment>
        </securitiesToBeSold>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>07/02/2021</acquiredDate>
            <natureOfAcquisitionTransaction>Open Market Purchase</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Open Market</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>157</amountOfSecuritiesAcquired>
            <paymentDate>07/02/2021</paymentDate>
            <natureOfPayment>Cash</natureOfPayment>
        </securitiesToBeSold>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>12/31/2018</acquiredDate>
            <natureOfAcquisitionTransaction>Open Market Purchase</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Open Market</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>500</amountOfSecuritiesAcquired>
            <paymentDate>12/31/2018</paymentDate>
            <natureOfPayment>Cash</natureOfPayment>
        </securitiesToBeSold>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>02/19/2008</acquiredDate>
            <natureOfAcquisitionTransaction>Open Market Purchase</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Open Market</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>122</amountOfSecuritiesAcquired>
            <paymentDate>02/19/2008</paymentDate>
            <natureOfPayment>Cash</natureOfPayment>
        </securitiesToBeSold>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>08/14/2008</acquiredDate>
            <natureOfAcquisitionTransaction>Open Market Purchase</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Open Market</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>221</amountOfSecuritiesAcquired>
            <paymentDate>08/14/2008</paymentDate>
            <natureOfPayment>Cash</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>CAROLINE E FUNK REV TRUST</name>
                <address>
                    <ns2:street1>102 South Clinton Street</ns2:street1>
                    <ns2:city>Iowa City</ns2:city>
                    <ns2:stateOrCountry>IA</ns2:stateOrCountry>
                    <ns2:zipCode>52240</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>05/03/2024</saleDate>
            <amountOfSecuritiesSold>343</amountOfSecuritiesSold>
            <grossProceeds>7340.20</grossProceeds>
        </securitiesSoldInPast3Months>
        <noticeSignature>
            <noticeDate>05/06/2024</noticeDate>
            <planAdoptionDates/>
            <signature>/s/ Charles N Funk</signature>
        </noticeSignature>
    </formData>
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144
0001430014
XXXXXXXX
LIVE
0001412665
MIDWESTONE FINANCIAL GROUP, INC.
001-35968
102 South Clinton Street
Iowa City
IA
52240
319-356-5800
CAROLINE E FUNK REV TRUST
Director
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
8th Floor
New York
NY
10004
3000
64200.00
15750471
05/06/2024
NASDAQ
Common
03/19/2020
Open Market Purchase
Open Market
N
1000
03/19/2020
Cash
Common
04/10/2008
Open Market Purchase
Open Market
N
1000
04/10/2008
Cash
Common
07/02/2021
Open Market Purchase
Open Market
N
157
07/02/2021
Cash
Common
12/31/2018
Open Market Purchase
Open Market
N
500
12/31/2018
Cash
Common
02/19/2008
Open Market Purchase
Open Market
N
122
02/19/2008
Cash
Common
08/14/2008
Open Market Purchase
Open Market
N
221
08/14/2008
Cash
N
CAROLINE E FUNK REV TRUST
102 South Clinton Street
Iowa City
IA
52240
Common
05/03/2024
343
7340.20
05/06/2024
/s/ Charles N Funk