<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:ns2="http://www.sec.gov/edgar/common">
    <headerData>
        <submissionType>144</submissionType>
        <filerInfo>
            <filer>
                <filerCredentials>
                    <cik>0001556355</cik>
                    <ccc>XXXXXXXX</ccc>
                </filerCredentials>
            </filer>
            <liveTestFlag>LIVE</liveTestFlag>



        </filerInfo>
    </headerData>
    <formData>
        <issuerInfo>
            <issuerCik>0000834365</issuerCik>
            <issuerName>BIOLIFE SOLUTIONS INC</issuerName>
            <secFileNumber>001-36362</secFileNumber>
            <issuerAddress>
                <ns2:street1>3303 Monte Villa Parkway, Suite 310</ns2:street1>
                <ns2:city>Bothell</ns2:city>
                <ns2:stateOrCountry>WA</ns2:stateOrCountry>
                <ns2:zipCode>98021</ns2:zipCode>
            </issuerAddress>
            <issuerContactPhone>4254021400</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>ABY J MATHEW</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>Officer</relationshipToIssuer>
            </relationshipsToIssuer>
        </issuerInfo>
        <securitiesInformation>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
                </address>
            </brokerOrMarketmakerDetails>
            <noOfUnitsSold>103404</noOfUnitsSold>
            <aggregateMarketValue>2424823.80</aggregateMarketValue>
            <noOfUnitsOutstanding>46138201</noOfUnitsOutstanding>
            <approxSaleDate>09/18/2024</approxSaleDate>
            <securitiesExchangeName>NASDAQ</securitiesExchangeName>
        </securitiesInformation>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>04/15/2016</acquiredDate>
            <natureOfAcquisitionTransaction>RSA</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>40208</amountOfSecuritiesAcquired>
            <paymentDate>04/15/2016</paymentDate>
            <natureOfPayment>N/A</natureOfPayment>
        </securitiesToBeSold>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>09/18/2024</acquiredDate>
            <natureOfAcquisitionTransaction>Exercise of Stock Options</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>50000</amountOfSecuritiesAcquired>
            <paymentDate>09/18/2024</paymentDate>
            <natureOfPayment>Cash</natureOfPayment>
        </securitiesToBeSold>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>02/16/2021</acquiredDate>
            <natureOfAcquisitionTransaction>Performance Stock Units</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>13196</amountOfSecuritiesAcquired>
            <paymentDate>02/16/2021</paymentDate>
            <natureOfPayment>N/A</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <remarks>The securities to be sold were acquired upon the vesting of restricted stock units during the period of 04/15/2016 through 07/01/2021.
The securities to be sold will be received upon the exercise of stock options over the next three months. The dates of acquisition and payment will be the dates of exercise/sale.
</remarks>
        <noticeSignature>
            <noticeDate>09/18/2024</noticeDate>
            <planAdoptionDates>
                <planAdoptionDate>06/14/2024</planAdoptionDate>
            </planAdoptionDates>
            <signature>/s/ Aby J. Matthew</signature>
        </noticeSignature>
    </formData>
</edgarSubmission>
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144
0001556355
XXXXXXXX
LIVE
0000834365
BIOLIFE SOLUTIONS INC
001-36362
3303 Monte Villa Parkway, Suite 310
Bothell
WA
98021
4254021400
ABY J MATHEW
Officer
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
8th Floor
New York
NY
10004
103404
2424823.80
46138201
09/18/2024
NASDAQ
Common
04/15/2016
RSA
Issuer
N
40208
04/15/2016
N/A
Common
09/18/2024
Exercise of Stock Options
Issuer
N
50000
09/18/2024
Cash
Common
02/16/2021
Performance Stock Units
Issuer
N
13196
02/16/2021
N/A
Y
The securities to be sold were acquired upon the vesting of restricted stock units during the period of 04/15/2016 through 07/01/2021.
The securities to be sold will be received upon the exercise of stock options over the next three months. The dates of acquisition and payment will be the dates of exercise/sale.
09/18/2024
06/14/2024
/s/ Aby J. Matthew