<?xml version="1.0" encoding="UTF-8"?><ns2:edgarSubmission xmlns:com="http://www.sec.gov/edgar/common" xmlns:ns2="http://www.sec.gov/edgar/ownership">
    <ns2:headerData>
        <ns2:submissionType>144</ns2:submissionType>
        <ns2:filerInfo>
            <ns2:filer>
                <ns2:filerCredentials>
                    <ns2:cik>0001236397</ns2:cik>
                    <ns2:ccc>XXXXXXXX</ns2:ccc>
                </ns2:filerCredentials>
            </ns2:filer>
            <ns2:liveTestFlag>LIVE</ns2:liveTestFlag>



        </ns2:filerInfo>
    </ns2:headerData>
    <ns2:formData>
        <ns2:issuerInfo>
            <ns2:issuerCik>0001447362</ns2:issuerCik>
            <ns2:issuerName>Castle Biosciences, Inc.</ns2:issuerName>
            <ns2:secFileNumber>001-38984</ns2:secFileNumber>
            <ns2:issuerAddress>
                <com:street1>505 S FRIENDSWOOD DR</com:street1>
                <com:street2>SUITE 401</com:street2>
                <com:city>FRIENDSWOOD</com:city>
                <com:stateOrCountry>TX</com:stateOrCountry>
                <com:zipCode>77546</com:zipCode>
            </ns2:issuerAddress>
            <ns2:issuerContactPhone>1-866-788-9007</ns2:issuerContactPhone>
            <ns2:nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Daniel Bradbury</ns2:nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <ns2:relationshipsToIssuer>
                <ns2:relationshipToIssuer>Officer</ns2:relationshipToIssuer>
            </ns2:relationshipsToIssuer>
        </ns2:issuerInfo>
        <ns2:securitiesInformation>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:brokerOrMarketmakerDetails>
                <ns2:name>Goldman Sachs &amp; Co. LLC</ns2:name>
                <ns2:address>
                    <com:street1>200 West Street</com:street1>
                    <com:city>New York</com:city>
                    <com:stateOrCountry>NY</com:stateOrCountry>
                    <com:zipCode>10282</com:zipCode>
                </ns2:address>
            </ns2:brokerOrMarketmakerDetails>
            <ns2:noOfUnitsSold>15734</ns2:noOfUnitsSold>
            <ns2:aggregateMarketValue>484292.52</ns2:aggregateMarketValue>
            <ns2:noOfUnitsOutstanding>28007301</ns2:noOfUnitsOutstanding>
            <ns2:approxSaleDate>12/03/2024</ns2:approxSaleDate>
            <ns2:securitiesExchangeName>NASD</ns2:securitiesExchangeName>
        </ns2:securitiesInformation>
        <ns2:securitiesToBeSold>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:acquiredDate>06/06/2018</ns2:acquiredDate>
            <ns2:natureOfAcquisitionTransaction>Acquired from issuer in private transaction</ns2:natureOfAcquisitionTransaction>
            <ns2:nameOfPersonfromWhomAcquired>Issuer</ns2:nameOfPersonfromWhomAcquired>
            <ns2:isGiftTransaction>N</ns2:isGiftTransaction>
            <ns2:amountOfSecuritiesAcquired>10032</ns2:amountOfSecuritiesAcquired>
            <ns2:paymentDate>06/06/2018</ns2:paymentDate>
            <ns2:natureOfPayment>None</ns2:natureOfPayment>
        </ns2:securitiesToBeSold>
        <ns2:securitiesToBeSold>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:acquiredDate>12/08/2020</ns2:acquiredDate>
            <ns2:natureOfAcquisitionTransaction>Acquired as a gift</ns2:natureOfAcquisitionTransaction>
            <ns2:nameOfPersonfromWhomAcquired>Daniel Bradbury</ns2:nameOfPersonfromWhomAcquired>
            <ns2:isGiftTransaction>Y</ns2:isGiftTransaction>
            <ns2:donarAcquiredDate>09/12/2008</ns2:donarAcquiredDate>
            <ns2:amountOfSecuritiesAcquired>2851</ns2:amountOfSecuritiesAcquired>
            <ns2:paymentDate>12/08/2020</ns2:paymentDate>
            <ns2:natureOfPayment>None</ns2:natureOfPayment>
        </ns2:securitiesToBeSold>
        <ns2:securitiesToBeSold>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:acquiredDate>12/08/2020</ns2:acquiredDate>
            <ns2:natureOfAcquisitionTransaction>Acquired as a gift</ns2:natureOfAcquisitionTransaction>
            <ns2:nameOfPersonfromWhomAcquired>Daniel Bradbury</ns2:nameOfPersonfromWhomAcquired>
            <ns2:isGiftTransaction>Y</ns2:isGiftTransaction>
            <ns2:donarAcquiredDate>09/12/2008</ns2:donarAcquiredDate>
            <ns2:amountOfSecuritiesAcquired>2851</ns2:amountOfSecuritiesAcquired>
            <ns2:paymentDate>12/08/2020</ns2:paymentDate>
            <ns2:natureOfPayment>None</ns2:natureOfPayment>
        </ns2:securitiesToBeSold>
        <ns2:nothingToReportFlagOnSecuritiesSoldInPast3Months>N</ns2:nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>The Daniel M. Bradbury Irrevocable Descendants Trust</ns2:name>
                <ns2:address>
                    <com:street1>505 S FRIENDSWOOD DR</com:street1>
                    <com:street2>SUITE 401</com:street2>
                    <com:city>FRIENDSWOOD</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>10/07/2024</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>1425</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>41955.99</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>The Annette E. Bradbury Irrevocable Descendants Trust</ns2:name>
                <ns2:address>
                    <com:street1>505 S FRIENDSWOOD DR</com:street1>
                    <com:street2>SUITE 401</com:street2>
                    <com:city>FRIENDSWOOD</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>10/07/2024</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>1425</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>41955.99</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>BioBrit, LLC</ns2:name>
                <ns2:address>
                    <com:street1>505 S FRIENDSWOOD DR</com:street1>
                    <com:street2>SUITE 401</com:street2>
                    <com:city>FRIENDSWOOD</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>10/07/2024</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>5017</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>147714.53</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>The Daniel M. Bradbury Irrevocable Descendants Trust</ns2:name>
                <ns2:address>
                    <com:street1>505 S FRIENDSWOOD DR</com:street1>
                    <com:street2>SUITE 401</com:street2>
                    <com:city>FRIENDSWOOD</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>09/05/2024</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>1425</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>41359.34</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>The Annette E. Bradbury Irrevocable Descendants Trust</ns2:name>
                <ns2:address>
                    <com:street1>505 S FRIENDSWOOD DR</com:street1>
                    <com:street2>SUITE 401</com:street2>
                    <com:city>FRIENDSWOOD</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>09/05/2024</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>1425</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>41359.34</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>BioBrit, LLC</ns2:name>
                <ns2:address>
                    <com:street1>505 S FRIENDSWOOD DR</com:street1>
                    <com:street2>SUITE 401</com:street2>
                    <com:city>FRIENDSWOOD</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>09/05/2024</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>5017</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>145613.91</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>The Daniel M. Bradbury Irrevocable Descendants Trust</ns2:name>
                <ns2:address>
                    <com:street1>505 S FRIENDSWOOD DR</com:street1>
                    <com:street2>SUITE 401</com:street2>
                    <com:city>FRIENDSWOOD</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>11/01/2024</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>1425</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>49495.95</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>The Annette E. Bradbury Irrevocable Descendants Trust</ns2:name>
                <ns2:address>
                    <com:street1>505 S FRIENDSWOOD DR</com:street1>
                    <com:street2>SUITE 401</com:street2>
                    <com:city>FRIENDSWOOD</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>11/01/2024</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>1425</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>49495.95</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>BioBrit, LLC</ns2:name>
                <ns2:address>
                    <com:street1>505 S FRIENDSWOOD DR</com:street1>
                    <com:street2>SUITE 401</com:street2>
                    <com:city>FRIENDSWOOD</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>11/01/2024</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>5017</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>174260.48</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:remarks>Total number of shares to be sold includes 10,032 shares by BioBrit, LLC, 2,851 shares by The Daniel M. Bradbury Irrevocable Descendants Trust, 2,851  shares by The Annette E. Bradbury Irrevocable Descendants Trust. The sales set forth herein are made in connection with a selling plan dated 5/10/2024, that is intended to comply with Rule 105-1 (c).</ns2:remarks>
        <ns2:noticeSignature>
            <ns2:noticeDate>12/03/2024</ns2:noticeDate>
            <ns2:planAdoptionDates>
                <ns2:planAdoptionDate>05/10/2024</ns2:planAdoptionDate>
            </ns2:planAdoptionDates>
            <ns2:signature>Goldman Sachs &amp; Co. LLC on behalf of Daniel Bradbury</ns2:signature>
        </ns2:noticeSignature>
    </ns2:formData>
</ns2:edgarSubmission>
[{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 13108}]
144
0001236397
XXXXXXXX
LIVE
0001447362
Castle Biosciences, Inc.
001-38984
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
1-866-788-9007
Daniel Bradbury
Officer
Common
Goldman Sachs & Co. LLC
200 West Street
New York
NY
10282
15734
484292.52
28007301
12/03/2024
NASD
Common
06/06/2018
Acquired from issuer in private transaction
Issuer
N
10032
06/06/2018
None
Common
12/08/2020
Acquired as a gift
Daniel Bradbury
Y
09/12/2008
2851
12/08/2020
None
Common
12/08/2020
Acquired as a gift
Daniel Bradbury
Y
09/12/2008
2851
12/08/2020
None
N
The Daniel M. Bradbury Irrevocable Descendants Trust
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
Common
10/07/2024
1425
41955.99
The Annette E. Bradbury Irrevocable Descendants Trust
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
Common
10/07/2024
1425
41955.99
BioBrit, LLC
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
Common
10/07/2024
5017
147714.53
The Daniel M. Bradbury Irrevocable Descendants Trust
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
Common
09/05/2024
1425
41359.34
The Annette E. Bradbury Irrevocable Descendants Trust
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
Common
09/05/2024
1425
41359.34
BioBrit, LLC
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
Common
09/05/2024
5017
145613.91
The Daniel M. Bradbury Irrevocable Descendants Trust
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
Common
11/01/2024
1425
49495.95
The Annette E. Bradbury Irrevocable Descendants Trust
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
Common
11/01/2024
1425
49495.95
BioBrit, LLC
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
Common
11/01/2024
5017
174260.48
Total number of shares to be sold includes 10,032 shares by BioBrit, LLC, 2,851 shares by The Daniel M. Bradbury Irrevocable Descendants Trust, 2,851  shares by The Annette E. Bradbury Irrevocable Descendants Trust. The sales set forth herein are made in connection with a selling plan dated 5/10/2024, that is intended to comply with Rule 105-1 (c).
12/03/2024
05/10/2024
Goldman Sachs & Co. LLC on behalf of Daniel Bradbury