<?xml version="1.0" encoding="UTF-8"?><ns2:edgarSubmission xmlns:com="http://www.sec.gov/edgar/common" xmlns:ns2="http://www.sec.gov/edgar/ownership">
    <ns2:headerData>
        <ns2:submissionType>144</ns2:submissionType>
        <ns2:filerInfo>
            <ns2:filer>
                <ns2:filerCredentials>
                    <ns2:cik>0001236397</ns2:cik>
                    <ns2:ccc>XXXXXXXX</ns2:ccc>
                </ns2:filerCredentials>
            </ns2:filer>
            <ns2:liveTestFlag>LIVE</ns2:liveTestFlag>



        </ns2:filerInfo>
    </ns2:headerData>
    <ns2:formData>
        <ns2:issuerInfo>
            <ns2:issuerCik>0001447362</ns2:issuerCik>
            <ns2:issuerName>Castle Biosciences, Inc.</ns2:issuerName>
            <ns2:secFileNumber>001-38984</ns2:secFileNumber>
            <ns2:issuerAddress>
                <com:street1>505 S FRIENDSWOOD DR</com:street1>
                <com:street2>SUITE 401</com:street2>
                <com:city>FRIENDSWOOD</com:city>
                <com:stateOrCountry>TX</com:stateOrCountry>
                <com:zipCode>77546</com:zipCode>
            </ns2:issuerAddress>
            <ns2:issuerContactPhone>1-866-788-9007</ns2:issuerContactPhone>
            <ns2:nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>Daniel Bradbury</ns2:nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <ns2:relationshipsToIssuer>
                <ns2:relationshipToIssuer>Affiliate</ns2:relationshipToIssuer>
            </ns2:relationshipsToIssuer>
        </ns2:issuerInfo>
        <ns2:securitiesInformation>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:brokerOrMarketmakerDetails>
                <ns2:name>Goldman Sachs &amp; Co. LLC</ns2:name>
                <ns2:address>
                    <com:street1>200 West Street</com:street1>
                    <com:city>New York</com:city>
                    <com:stateOrCountry>NY</com:stateOrCountry>
                    <com:zipCode>10282</com:zipCode>
                </ns2:address>
            </ns2:brokerOrMarketmakerDetails>
            <ns2:noOfUnitsSold>75000</ns2:noOfUnitsSold>
            <ns2:aggregateMarketValue>1522500</ns2:aggregateMarketValue>
            <ns2:noOfUnitsOutstanding>26691286</ns2:noOfUnitsOutstanding>
            <ns2:approxSaleDate>07/07/2023</ns2:approxSaleDate>
            <ns2:securitiesExchangeName>NASD</ns2:securitiesExchangeName>
        </ns2:securitiesInformation>
        <ns2:securitiesToBeSold>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:acquiredDate>12/08/2020</ns2:acquiredDate>
            <ns2:natureOfAcquisitionTransaction>Acquired as a gift</ns2:natureOfAcquisitionTransaction>
            <ns2:nameOfPersonfromWhomAcquired>Daniel M. Bradbury</ns2:nameOfPersonfromWhomAcquired>
            <ns2:isGiftTransaction>Y</ns2:isGiftTransaction>
            <ns2:donarAcquiredDate>08/12/2014</ns2:donarAcquiredDate>
            <ns2:amountOfSecuritiesAcquired>15000</ns2:amountOfSecuritiesAcquired>
            <ns2:paymentDate>12/08/2020</ns2:paymentDate>
            <ns2:natureOfPayment>N/A</ns2:natureOfPayment>
        </ns2:securitiesToBeSold>
        <ns2:securitiesToBeSold>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:acquiredDate>12/08/2020</ns2:acquiredDate>
            <ns2:natureOfAcquisitionTransaction>Acquired as a gift</ns2:natureOfAcquisitionTransaction>
            <ns2:nameOfPersonfromWhomAcquired>Daniel M. Bradbury</ns2:nameOfPersonfromWhomAcquired>
            <ns2:isGiftTransaction>Y</ns2:isGiftTransaction>
            <ns2:donarAcquiredDate>08/12/2014</ns2:donarAcquiredDate>
            <ns2:amountOfSecuritiesAcquired>15000</ns2:amountOfSecuritiesAcquired>
            <ns2:paymentDate>12/08/2020</ns2:paymentDate>
            <ns2:natureOfPayment>N/A</ns2:natureOfPayment>
        </ns2:securitiesToBeSold>
        <ns2:securitiesToBeSold>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:acquiredDate>01/03/2018</ns2:acquiredDate>
            <ns2:natureOfAcquisitionTransaction>Acquired from issuer in private transaction</ns2:natureOfAcquisitionTransaction>
            <ns2:nameOfPersonfromWhomAcquired>Issuer</ns2:nameOfPersonfromWhomAcquired>
            <ns2:isGiftTransaction>N</ns2:isGiftTransaction>
            <ns2:amountOfSecuritiesAcquired>45000</ns2:amountOfSecuritiesAcquired>
            <ns2:paymentDate>01/03/2018</ns2:paymentDate>
            <ns2:natureOfPayment>N/A</ns2:natureOfPayment>
        </ns2:securitiesToBeSold>
        <ns2:nothingToReportFlagOnSecuritiesSoldInPast3Months>N</ns2:nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>The Daniel M. Bradbury Irrevocable Descendants Trust</ns2:name>
                <ns2:address>
                    <com:street1>505 S FRIENDSWOOD DR</com:street1>
                    <com:street2>SUITE 401</com:street2>
                    <com:city>FRIENDSWOOD</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>05/05/2023</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>1471</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>31606.79</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>The Annette E. Bradbury Irrevocable Descendants Trust</ns2:name>
                <ns2:address>
                    <com:street1>505 S FRIENDSWOOD DR</com:street1>
                    <com:street2>SUITE 401</com:street2>
                    <com:city>FRIENDSWOOD</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>05/05/2023</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>1471</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>31606.79</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>BioBrit, LLC</ns2:name>
                <ns2:address>
                    <com:street1>505 S FRIENDSWOOD DR</com:street1>
                    <com:street2>SUITE 401</com:street2>
                    <com:city>FRIENDSWOOD</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>05/05/2023</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>4415</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>94863.34</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>The Daniel M. Bradbury Irrevocable Descendants Trust</ns2:name>
                <ns2:address>
                    <com:street1>505 S FRIENDSWOOD DR</com:street1>
                    <com:street2>SUITE 401</com:street2>
                    <com:city>FRIENDSWOOD</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>05/04/2023</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>3529</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>76542.6</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>The Annette E. Bradbury Irrevocable Descendants Trust</ns2:name>
                <ns2:address>
                    <com:street1>505 S FRIENDSWOOD DR</com:street1>
                    <com:street2>SUITE 401</com:street2>
                    <com:city>FRIENDSWOOD</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>05/04/2023</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>3529</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>76542.6</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:securitiesSoldInPast3Months>
            <ns2:sellerDetails>
                <ns2:name>BioBrit, LLC</ns2:name>
                <ns2:address>
                    <com:street1>505 S FRIENDSWOOD DR</com:street1>
                    <com:street2>SUITE 401</com:street2>
                    <com:city>FRIENDSWOOD</com:city>
                    <com:stateOrCountry>TX</com:stateOrCountry>
                    <com:zipCode>77546</com:zipCode>
                </ns2:address>
            </ns2:sellerDetails>
            <ns2:securitiesClassTitle>Common</ns2:securitiesClassTitle>
            <ns2:saleDate>05/04/2023</ns2:saleDate>
            <ns2:amountOfSecuritiesSold>10585</ns2:amountOfSecuritiesSold>
            <ns2:grossProceeds>229584.42</ns2:grossProceeds>
        </ns2:securitiesSoldInPast3Months>
        <ns2:remarks>Total number of shares to be sold includes 45,000 shares by BioBrit, LLC, 15,000 shares by The Daniel M. Bradbury Irrevocable Descendants Trust, 15,000 shares by The Annette E. Bradbury
Irrevocable Descendants Trust. The sales set forth herein are made in connection with a selling plan dated 11/23/2022, that is intended to comply with Rule 105-1 (c).</ns2:remarks>
        <ns2:noticeSignature>
            <ns2:noticeDate>07/07/2023</ns2:noticeDate>
            <ns2:planAdoptionDates>
                <ns2:planAdoptionDate>11/23/2022</ns2:planAdoptionDate>
            </ns2:planAdoptionDates>
            <ns2:signature>Goldman Sachs &amp; Co. LLC on behalf of Daniel M. Bradbury</ns2:signature>
        </ns2:noticeSignature>
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144
0001236397
XXXXXXXX
LIVE
0001447362
Castle Biosciences, Inc.
001-38984
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
1-866-788-9007
Daniel Bradbury
Affiliate
Common
Goldman Sachs & Co. LLC
200 West Street
New York
NY
10282
75000
1522500
26691286
07/07/2023
NASD
Common
12/08/2020
Acquired as a gift
Daniel M. Bradbury
Y
08/12/2014
15000
12/08/2020
N/A
Common
12/08/2020
Acquired as a gift
Daniel M. Bradbury
Y
08/12/2014
15000
12/08/2020
N/A
Common
01/03/2018
Acquired from issuer in private transaction
Issuer
N
45000
01/03/2018
N/A
N
The Daniel M. Bradbury Irrevocable Descendants Trust
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
Common
05/05/2023
1471
31606.79
The Annette E. Bradbury Irrevocable Descendants Trust
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
Common
05/05/2023
1471
31606.79
BioBrit, LLC
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
Common
05/05/2023
4415
94863.34
The Daniel M. Bradbury Irrevocable Descendants Trust
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
Common
05/04/2023
3529
76542.6
The Annette E. Bradbury Irrevocable Descendants Trust
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
Common
05/04/2023
3529
76542.6
BioBrit, LLC
505 S FRIENDSWOOD DR
SUITE 401
FRIENDSWOOD
TX
77546
Common
05/04/2023
10585
229584.42
Total number of shares to be sold includes 45,000 shares by BioBrit, LLC, 15,000 shares by The Daniel M. Bradbury Irrevocable Descendants Trust, 15,000 shares by The Annette E. Bradbury
Irrevocable Descendants Trust. The sales set forth herein are made in connection with a selling plan dated 11/23/2022, that is intended to comply with Rule 105-1 (c).
07/07/2023
11/23/2022
Goldman Sachs & Co. LLC on behalf of Daniel M. Bradbury