<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
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    <filerInfo>
      <filer>
        <filerCredentials>
          <cik>0001854968</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>



    </filerInfo>
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    <issuerInfo>
      <issuerCik>0000834365</issuerCik>
      <issuerName>BIOLIFE SOLUTIONS INC</issuerName>
      <secFileNumber>001-36362</secFileNumber>
      <issuerAddress>
        <com:street1>3303 MONTE VILLA PARKWAY</com:street1>
        <com:street2>SUITE 310</com:street2>
        <com:city>BOTHELL</com:city>
        <com:stateOrCountry>WA</com:stateOrCountry>
        <com:zipCode>98021</com:zipCode>
      </issuerAddress>
      <issuerContactPhone>4254021400</issuerContactPhone>
      <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>DuRoss Amy</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <relationshipsToIssuer>
        <relationshipToIssuer>Director</relationshipToIssuer>
      </relationshipsToIssuer>
    </issuerInfo>
    <securitiesInformation>
      <securitiesClassTitle>Common Stock</securitiesClassTitle>
      <brokerOrMarketmakerDetails>
        <name>Charles Schwab &amp; Co.</name>
        <address>
          <com:street1>3000 Schwab Way</com:street1>
          <com:city>Westlake</com:city>
          <com:stateOrCountry>TX</com:stateOrCountry>
          <com:zipCode>76262</com:zipCode>
        </address>
      </brokerOrMarketmakerDetails>
      <noOfUnitsSold>11200</noOfUnitsSold>
      <aggregateMarketValue>190176.00</aggregateMarketValue>
      <noOfUnitsOutstanding>45300000</noOfUnitsOutstanding>
      <approxSaleDate>03/12/2024</approxSaleDate>
      <securitiesExchangeName>NASDAQ</securitiesExchangeName>
    </securitiesInformation>
    <securitiesToBeSold>
      <securitiesClassTitle>Common Stock</securitiesClassTitle>
      <acquiredDate>04/13/2022</acquiredDate>
      <natureOfAcquisitionTransaction>Equity Compensation</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>11200</amountOfSecuritiesAcquired>
      <paymentDate>04/13/2022</paymentDate>
      <natureOfPayment>N/A</natureOfPayment>
    </securitiesToBeSold>
    <nothingToReportFlagOnSecuritiesSoldInPast3Months>Y</nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <remarks>Shares to be sold were acquired between 04/13/2022-06/12/2023</remarks>
    <noticeSignature>
      <noticeDate>03/12/2024</noticeDate>
      <signature>Amy Duross</signature>
    </noticeSignature>
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144
0001854968
XXXXXXXX
LIVE
0000834365
BIOLIFE SOLUTIONS INC
001-36362
3303 MONTE VILLA PARKWAY
SUITE 310
BOTHELL
WA
98021
4254021400
DuRoss Amy
Director
Common Stock
Charles Schwab & Co.
3000 Schwab Way
Westlake
TX
76262
11200
190176.00
45300000
03/12/2024
NASDAQ
Common Stock
04/13/2022
Equity Compensation
Issuer
N
11200
04/13/2022
N/A
Y
Shares to be sold were acquired between 04/13/2022-06/12/2023
03/12/2024
Amy Duross