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        <filerInfo>
            <filer>
                <filerCredentials>
                    <cik>0001276311</cik>
                    <ccc>XXXXXXXX</ccc>
                </filerCredentials>
            </filer>
            <liveTestFlag>LIVE</liveTestFlag>



        </filerInfo>
    </headerData>
    <formData>
        <issuerInfo>
            <issuerCik>0001625101</issuerCik>
            <issuerName>PULSE BIOSCIENCES, INC</issuerName>
            <secFileNumber>001-37744</secFileNumber>
            <issuerAddress>
                <ns2:street1>601 Brickell Key Drive</ns2:street1>
                <ns2:city>Miami</ns2:city>
                <ns2:stateOrCountry>FL</ns2:stateOrCountry>
                <ns2:zipCode>33131</ns2:zipCode>
            </issuerAddress>
            <issuerContactPhone>510-905-1106</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>DARRIN UECKER</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>Officer</relationshipToIssuer>
                <relationshipToIssuer>Director</relationshipToIssuer>
            </relationshipsToIssuer>
        </issuerInfo>
        <securitiesInformation>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
                </address>
            </brokerOrMarketmakerDetails>
            <noOfUnitsSold>15000</noOfUnitsSold>
            <aggregateMarketValue>239740.50</aggregateMarketValue>
            <noOfUnitsOutstanding>67273862</noOfUnitsOutstanding>
            <approxSaleDate>06/13/2025</approxSaleDate>
            <securitiesExchangeName>NASDAQ</securitiesExchangeName>
        </securitiesInformation>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>12/29/2021</acquiredDate>
            <natureOfAcquisitionTransaction>Restricted stock vesting under a registered plan</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>15000</amountOfSecuritiesAcquired>
            <paymentDate>12/29/2021</paymentDate>
            <natureOfPayment>Services Rendered</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>DARRIN UECKER</name>
                <address>
                    <ns2:street1>849 Mitten Road, Suite 104</ns2:street1>
                    <ns2:city>Burlingame</ns2:city>
                    <ns2:stateOrCountry>CA</ns2:stateOrCountry>
                    <ns2:zipCode>94010</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>06/12/2025</saleDate>
            <amountOfSecuritiesSold>15000</amountOfSecuritiesSold>
            <grossProceeds>246048.00</grossProceeds>
        </securitiesSoldInPast3Months>
        <noticeSignature>
            <noticeDate>06/13/2025</noticeDate>
            <planAdoptionDates/>
            <signature>/s/ Darrin R Uecker</signature>
        </noticeSignature>
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144
0001276311
XXXXXXXX
LIVE
0001625101
PULSE BIOSCIENCES, INC
001-37744
601 Brickell Key Drive
Miami
FL
33131
510-905-1106
DARRIN UECKER
Officer
Director
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
8th Floor
New York
NY
10004
15000
239740.50
67273862
06/13/2025
NASDAQ
Common
12/29/2021
Restricted stock vesting under a registered plan
Issuer
N
15000
12/29/2021
Services Rendered
N
DARRIN UECKER
849 Mitten Road, Suite 104
Burlingame
CA
94010
Common
06/12/2025
15000
246048.00
06/13/2025
/s/ Darrin R Uecker