<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:ns2="http://www.sec.gov/edgar/common">
    <headerData>
        <submissionType>144</submissionType>
        <filerInfo>
            <filer>
                <filerCredentials>
                    <cik>0001754464</cik>
                    <ccc>XXXXXXXX</ccc>
                </filerCredentials>
            </filer>
            <liveTestFlag>LIVE</liveTestFlag>



        </filerInfo>
    </headerData>
    <formData>
        <issuerInfo>
            <issuerCik>0001717115</issuerCik>
            <issuerName>Tempus AI</issuerName>
            <secFileNumber>001-42130</secFileNumber>
            <issuerAddress>
                <ns2:street1>600 WEST CHICAGO AVENUE, Suite 510</ns2:street1>
                <ns2:city>CHICAGO</ns2:city>
                <ns2:stateOrCountry>IL</ns2:stateOrCountry>
                <ns2:zipCode>60654</ns2:zipCode>
            </issuerAddress>
            <issuerContactPhone>8009765448</issuerContactPhone>
            <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>RYAN BARTOLUCCI</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
            <relationshipsToIssuer>
                <relationshipToIssuer>Officer</relationshipToIssuer>
            </relationshipsToIssuer>
        </issuerInfo>
        <securitiesInformation>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <brokerOrMarketmakerDetails>
                <name>Morgan Stanley Smith Barney LLC Executive Financial Services</name>
                <address>
                    <ns2:street1>1 New York Plaza</ns2:street1>
                    <ns2:street2>8th Floor</ns2:street2>
                    <ns2:city>New York</ns2:city>
                    <ns2:stateOrCountry>NY</ns2:stateOrCountry>
                    <ns2:zipCode>10004</ns2:zipCode>
                </address>
            </brokerOrMarketmakerDetails>
            <noOfUnitsSold>13770</noOfUnitsSold>
            <aggregateMarketValue>787506.30</aggregateMarketValue>
            <noOfUnitsOutstanding>152401894</noOfUnitsOutstanding>
            <approxSaleDate>01/31/2025</approxSaleDate>
            <securitiesExchangeName>NASDAQ</securitiesExchangeName>
        </securitiesInformation>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>07/14/2021</acquiredDate>
            <natureOfAcquisitionTransaction>Restricted Stock Units</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>10850</amountOfSecuritiesAcquired>
            <paymentDate>07/14/2021</paymentDate>
            <natureOfPayment>N/A</natureOfPayment>
        </securitiesToBeSold>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>04/27/2022</acquiredDate>
            <natureOfAcquisitionTransaction>Restricted Stock Units</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>1879</amountOfSecuritiesAcquired>
            <paymentDate>04/27/2022</paymentDate>
            <natureOfPayment>N/A</natureOfPayment>
        </securitiesToBeSold>
        <securitiesToBeSold>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <acquiredDate>04/18/2023</acquiredDate>
            <natureOfAcquisitionTransaction>Restricted Stock Units</natureOfAcquisitionTransaction>
            <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
            <isGiftTransaction>N</isGiftTransaction>
            <amountOfSecuritiesAcquired>1041</amountOfSecuritiesAcquired>
            <paymentDate>04/18/2023</paymentDate>
            <natureOfPayment>N/A</natureOfPayment>
        </securitiesToBeSold>
        <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
        <securitiesSoldInPast3Months>
            <sellerDetails>
                <name>RYAN BARTOLUCCI</name>
                <address>
                    <ns2:street1>600 WEST CHICAGO AVENUE, Suite 510</ns2:street1>
                    <ns2:city>CHICAGO</ns2:city>
                    <ns2:stateOrCountry>IL</ns2:stateOrCountry>
                    <ns2:zipCode>60654</ns2:zipCode>
                </address>
            </sellerDetails>
            <securitiesClassTitle>Common</securitiesClassTitle>
            <saleDate>12/16/2024</saleDate>
            <amountOfSecuritiesSold>339</amountOfSecuritiesSold>
            <grossProceeds>13637.97</grossProceeds>
        </securitiesSoldInPast3Months>
        <noticeSignature>
            <noticeDate>01/31/2025</noticeDate>
            <planAdoptionDates>
                <planAdoptionDate>06/14/2024</planAdoptionDate>
            </planAdoptionDates>
            <signature>/s/ Ryan Bartolucci</signature>
        </noticeSignature>
    </formData>
</edgarSubmission>
[{"type": "144", "sequence": "1", "filename": "primary_doc.xml", "secsgml_size_bytes": 5176}]
144
0001754464
XXXXXXXX
LIVE
0001717115
Tempus AI
001-42130
600 WEST CHICAGO AVENUE, Suite 510
CHICAGO
IL
60654
8009765448
RYAN BARTOLUCCI
Officer
Common
Morgan Stanley Smith Barney LLC Executive Financial Services
1 New York Plaza
8th Floor
New York
NY
10004
13770
787506.30
152401894
01/31/2025
NASDAQ
Common
07/14/2021
Restricted Stock Units
Issuer
N
10850
07/14/2021
N/A
Common
04/27/2022
Restricted Stock Units
Issuer
N
1879
04/27/2022
N/A
Common
04/18/2023
Restricted Stock Units
Issuer
N
1041
04/18/2023
N/A
N
RYAN BARTOLUCCI
600 WEST CHICAGO AVENUE, Suite 510
CHICAGO
IL
60654
Common
12/16/2024
339
13637.97
01/31/2025
06/14/2024
/s/ Ryan Bartolucci