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<P STYLE="text-align: center; margin-top: 0; margin-bottom: 0">&nbsp;<B>As filed with the U.S. Securities and Exchange Commission on March 22, 2023.</B></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 2pt 22pt 0 0; text-align: right"><B>Registration No. 333-_______</B></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 2pt 22pt 0 0; text-align: right">&nbsp;</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 4pt 0 0; text-align: center"><B>UNITED STATES<BR>
SECURITIES AND EXCHANGE COMMISSION<BR>
Washington, D.C. 20549</B></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 4pt 0 0; text-align: center"><B>____________________________</B></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-align: center"><B>FORM S-8<BR>
REGISTRATION STATEMENT<BR>
UNDER<BR>
THE SECURITIES ACT OF 1933</B></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 2pt 0 0; text-align: center"><B>____________________________</B></P>

<P STYLE="font: 12pt Times New Roman, Times, Serif; margin: 8pt 0 0; text-align: center"><FONT STYLE="font-size: 16pt"><B>CARDIO DIAGNOSTICS
HOLDINGS, INC.<BR>
</B></FONT><FONT STYLE="font-size: 10pt">(Exact name of registrant as specified in its charter)</FONT></P>

<P STYLE="font: 12pt Times New Roman, Times, Serif; margin: 8pt 0 0; text-align: center"><FONT STYLE="font-size: 10pt">&nbsp;</FONT></P>

<TABLE CELLSPACING="0" CELLPADDING="0" STYLE="font: 12pt Times New Roman, Times, Serif; width: 100%; border-collapse: collapse">
  <TR STYLE="vertical-align: bottom">
    <TD STYLE="width: 32%; border-bottom: Black 1pt solid; text-align: center"><FONT STYLE="font-size: 9pt"><B>Delaware</B></FONT></TD>
    <TD STYLE="white-space: nowrap; width: 1%">&nbsp;</TD>
    <TD STYLE="width: 32%; border-bottom: Black 1pt solid; text-align: center"><FONT STYLE="font-size: 9pt"><B>6770</B></FONT></TD>
    <TD STYLE="white-space: nowrap; width: 1%">&nbsp;</TD>
    <TD STYLE="width: 34%; border-bottom: Black 1pt solid; text-align: center"><FONT STYLE="font-size: 10pt"><B>87-0925574</B></FONT></TD></TR>
  <TR STYLE="vertical-align: bottom">
    <TD STYLE="text-align: center"><FONT STYLE="font-size: 10pt">(State or other jurisdiction of<BR>
incorporation or organization)</FONT></TD>
    <TD STYLE="white-space: nowrap">&nbsp;</TD>
    <TD STYLE="text-align: center"><FONT STYLE="font-size: 10pt">(Primary Standard Industrial<BR>
Classification Code Number)</FONT></TD>
    <TD STYLE="white-space: nowrap">&nbsp;</TD>
    <TD STYLE="text-align: center"><FONT STYLE="font-size: 10pt">(I.R.S. Employer<BR>
Identification No.)</FONT></TD></TR>
  </TABLE>
<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 8pt 0; text-align: center"><B>400 North Aberdeen Street, Suite 900<BR>
Chicago, IL 60642<BR>
Telephone: (855) 226-9991<BR>
</B>(Address, including zip code, and telephone number, including area code, of registrant&rsquo;s principal executive offices)</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-align: center"><B>Cardio Diagnostics Holdings, Inc. 2022 Equity Incentive
Plan</B></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-align: center">(Full Title of the Plan)</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 4pt 0; text-align: center"><B>____________________________</B></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 2pt 0 0; text-align: center"><B>Meeshanthini V. Dogan, Ph.D.<BR>
Chief Executive Officer<BR>
Cardio Diagnostics Holdings, Inc.<BR>
400 North Aberdeen Street, Suite 900<BR>
Chicago, IL 60642<BR>
Telephone: (855) 226-9991<BR>
</B>(Name, address, including zip code, and telephone number, including area code, of agent for service)</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 4pt 0; text-align: center"><B>____________________________</B></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 4pt 0 0; text-align: center"><B>Copies to:</B></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 4pt 0 0; text-align: center"><B>P. Rupert Russell, Esq.<BR>
Shartsis Friese LLP<BR>
One Maritime Plaza, 18th Floor<BR>
San Francisco, CA 94111<BR>
(415) 421-6500</B></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 4pt 0 0; text-align: center"><B>&nbsp;</B></P>

<P STYLE="font: 9pt Times New Roman, Times, Serif; margin: 4pt 0 0; text-indent: 24pt">Indicate by check mark whether the registrant is
a large accelerated filer, an accelerated filer, a non-accelerated&nbsp;filer, smaller reporting company, or an emerging growth company.
See the definitions of &ldquo;large accelerated filer,&rdquo; &ldquo;accelerated filer,&rdquo; &ldquo;smaller reporting company,&rdquo;
and &ldquo;emerging growth company&rdquo; in Rule 12b-2&nbsp;of the Exchange Act.</P>

<TABLE CELLSPACING="0" CELLPADDING="0" STYLE="font: 12pt Times New Roman, Times, Serif; width: 100%; border-collapse: collapse">
  <TR STYLE="vertical-align: bottom">
    <TD STYLE="width: 3%">&nbsp;</TD>
    <TD STYLE="white-space: nowrap; width: 1%">&nbsp;</TD>
    <TD STYLE="width: 20%; text-align: justify"><FONT STYLE="font-size: 9pt">Large accelerated filer</FONT></TD>
    <TD STYLE="white-space: nowrap; width: 1%">&nbsp;</TD>
    <TD STYLE="width: 19%; text-align: justify"><FONT STYLE="font-family: Segoe UI Symbol,sans-serif; font-size: 9pt">&#9744;</FONT></TD>
    <TD STYLE="white-space: nowrap; width: 1%">&nbsp;</TD>
    <TD STYLE="width: 21%"><FONT STYLE="font-size: 9pt">Accelerated&nbsp;filer</FONT></TD>
    <TD STYLE="white-space: nowrap; width: 1%">&nbsp;</TD>
    <TD STYLE="width: 33%; text-align: justify"><FONT STYLE="font-family: Segoe UI Symbol,sans-serif; font-size: 9pt">&#9744;</FONT></TD></TR>
  <TR STYLE="vertical-align: bottom">
    <TD>&nbsp;</TD>
    <TD STYLE="white-space: nowrap">&nbsp;</TD>
    <TD STYLE="text-align: justify"><FONT STYLE="font-size: 9pt">Non- accelerated filer</FONT></TD>
    <TD STYLE="white-space: nowrap">&nbsp;</TD>
    <TD STYLE="text-align: justify"><FONT STYLE="font-family: Segoe UI Symbol,sans-serif; font-size: 9pt">&#9746;</FONT></TD>
    <TD STYLE="white-space: nowrap">&nbsp;</TD>
    <TD><FONT STYLE="font-size: 9pt">Smaller&nbsp;reporting&nbsp;company</FONT></TD>
    <TD STYLE="white-space: nowrap">&nbsp;</TD>
    <TD STYLE="text-align: justify"><FONT STYLE="font-family: Segoe UI Symbol,sans-serif; font-size: 9pt">&#9746;</FONT></TD></TR>
  <TR STYLE="vertical-align: bottom">
    <TD>&nbsp;</TD>
    <TD STYLE="white-space: nowrap">&nbsp;</TD>
    <TD>&nbsp;</TD>
    <TD STYLE="white-space: nowrap">&nbsp;</TD>
    <TD>&nbsp;</TD>
    <TD STYLE="white-space: nowrap">&nbsp;</TD>
    <TD><FONT STYLE="font-size: 9pt">Emerging&nbsp;growth&nbsp;company</FONT></TD>
    <TD STYLE="white-space: nowrap">&nbsp;</TD>
    <TD STYLE="text-align: justify"><FONT STYLE="font-family: Segoe UI Symbol,sans-serif; font-size: 9pt">&#9746;</FONT></TD></TR>
  </TABLE>
<P STYLE="font: 9pt Times New Roman, Times, Serif; margin: 6pt 0 0; text-indent: 24pt">If an emerging growth company, indicate by check
mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting
standards provided pursuant to Section 7(a)(2)(B) of the Securities Act.&nbsp;<FONT STYLE="font-family: Segoe UI Symbol,sans-serif">&#9744;</FONT></P>

<P STYLE="font: 9pt Times New Roman, Times, Serif; margin: 6pt 0 0; text-indent: 24pt">&nbsp;</P>


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<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-align: center"><B>EXPLANATORY NOTE</B></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">This Registration Statement on Form S-8 is being filed by Cardio
Diagnostics Holdings, Inc. (the &ldquo;<B><I>Company</I></B>&rdquo; and the &ldquo;<B><I>Registrant</I></B>&rdquo;) for the purpose of
registering 3,265,516 shares of the Company&rsquo;s common stock, par value of $0.00001 per share, issuable under the Cardio Diagnostics
Holdings, Inc. 2022 Equity Incentive Plan (the &ldquo;<B><I>2022 Plan</I></B>&rdquo;).</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">On October 25, 2022, pursuant to that certain Business Combination
Agreement, dated as of May 27, 2022 as amended by Amendment No.&nbsp;1 to the Agreement, dated September 15, 2022 (the &ldquo;<B><I>Business
Combination Agreement</I></B>&rdquo;), by and among Mana Capital Acquisition Corp., a Delaware corporation (&ldquo;<B><I>Mana</I></B>&rdquo;),
Mana Merger Sub, Inc., a Delaware corporation and wholly-owned subsidiary of Mana (&ldquo;<B><I>Merger Sub</I></B>&rdquo;), Cardio Diagnostics,
Inc., a Delaware corporation (&ldquo;<B><I>Legacy Cardio</I></B>&rdquo;), and <FONT STYLE="font-family: Times New Roman PS Std,serif">Meeshanthini
Dogan, PhD, as the &ldquo;<B><I>Shareholders&rsquo; Representative</I></B>,&rdquo;</FONT> Merger Sub merged with and into Legacy Cardio,
with Legacy Cardio continuing as the surviving corporation, resulting in Legacy Cardio becoming a wholly-owned subsidiary of the Company.
The Registrant&rsquo;s Common Stock commenced trading on the Nasdaq Capital Market under the symbol &ldquo;CDIO&rdquo; on October 26,
2022.</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-align: center"><B>PART I<BR>
INFORMATION REQUIRED IN THE SECTION 10(a) PROSPECTUS</B></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">The documents containing the information specified in Items 1 and
2 of this Part I of Form S-8 (plan information and registration information and employee plan annual information) will be sent or given
to employees as specified by the Securities and Exchange Commission (the &ldquo;<B><I>Commission</I></B>&rdquo;) pursuant to Rule 428(b)(1)
of the Securities Act. Such documents are not required to be and are not filed with the Commission either as part of this registration
statement (this &ldquo;<B><I>Registration Statement</I></B>&rdquo;) or as prospectuses or prospectus supplements pursuant to Rule 424.
These documents and the documents incorporated by reference in this Registration Statement pursuant to Item 3 of Part II hereof, taken
together, constitute a prospectus that meets the requirements of Section 10(a) of the Securities Act. The Company will provide a written
statement to participants advising them of the availability without charge, upon written or oral request, of the documents incorporated
by reference in Item 3 of Part II hereof and including the statement in the preceding sentence. The written statement to all participants
will indicate the availability without charge, upon written or oral request, of other documents required to be delivered pursuant to Rule
428(b) and will include the address and telephone number to which the request is to be directed.</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-align: center"><B>PART II<BR>
INFORMATION REQUIRED IN THE REGISTRATION STATEMENT</B></P>

<TABLE CELLPADDING="0" CELLSPACING="0" WIDTH="100%" STYLE="font: 10pt Times New Roman, Times, Serif; margin-top: 0; margin-bottom: 12pt"><TR STYLE="vertical-align: top">
<TD STYLE="width: 0"></TD><TD STYLE="width: 64.6pt"><B>Item 3.</B></TD><TD><B>Incorporation of Documents by Reference.</B></TD></TR></TABLE>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">The following documents filed by Cardio Diagnostics Holdings, Inc.,
or its predecessor, Mana Capital Acquisition Corp., with the Commission pursuant to the Securities Exchange Act of 1934, as amended, (the
&ldquo;Exchange Act&rdquo;) are incorporated by reference in, and shall be deemed to be a part of, this Registration <FONT STYLE="letter-spacing: -0.1pt">Statement:</FONT></P>

<P STYLE="font: 11pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-indent: 0in"><FONT STYLE="font-size: 9pt; letter-spacing: -0.05pt">(a)</FONT><FONT STYLE="font-family: Times New Roman, Times, Serif; font-size: 7pt; letter-spacing: -0.05pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
</FONT><FONT STYLE="font-size: 10pt">the Registrant&rsquo;s Annual Report on <A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997322000372/mana_10k-123121.htm" STYLE="-sec-extract: exhibit">Form 10-K</A> for the fiscal year ended December 31, 2021, filed
with the Commission on March 31, 2022, which Report contains Mana&rsquo;s audited financial statements for the latest fiscal year for
which such statements have been filed;</FONT></P>

<P STYLE="font: 11pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-indent: 0in"><FONT STYLE="font-size: 9pt; letter-spacing: -0.05pt">(b)</FONT><FONT STYLE="font-family: Times New Roman, Times, Serif; font-size: 7pt; letter-spacing: -0.05pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
</FONT><FONT STYLE="font-size: 10pt">the Registrant&rsquo;s Quarterly Reports on Form 10-Q for the fiscal quarters ended <A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997322000575/mana_10q-033122.htm" STYLE="-sec-extract: exhibit">March 31, 2022</A>,
<A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997322000958/mana_10q-063022.htm" STYLE="-sec-extract: exhibit">June 30, 2022</A> and <A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997322001398/cdio_10q-093022.htm" STYLE="-sec-extract: exhibit">September 30, 2022</A>, filed with the Commission on May 12, 2022, August 10, 2022 and November 7, 2022, respectively;</FONT></P>

<P STYLE="font: 11pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-indent: 0in"><FONT STYLE="font-size: 9pt; letter-spacing: -0.05pt">(c)</FONT><FONT STYLE="font-family: Times New Roman, Times, Serif; font-size: 7pt; letter-spacing: -0.05pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
</FONT><FONT STYLE="font-size: 10pt">the Registrant&rsquo;s Current Reports on Form 8-K filed with the Commission on <A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997322000718/mana_8k-052722.htm" STYLE="-sec-extract: exhibit">May 31, 2022</A>, <A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997322001032/mana_8k-082522.htm" STYLE="-sec-extract: exhibit">August
25, 2022</A>, <A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997322001040/mana_8k-082622.htm" STYLE="-sec-extract: exhibit">August 26, 2022</A>, <A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997322001114/mana_8k-091522.htm" STYLE="-sec-extract: exhibit">September 15, 2022</A>, <A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997322001136/mana_8k-092022.htm" STYLE="-sec-extract: exhibit">September 21, 2022</A>, <A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997322001157/mana_8k-092322.htm" STYLE="-sec-extract: exhibit">September 23, 2022</A>, <A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997322001382/cdio_8k.htm" STYLE="-sec-extract: exhibit">October 31, 2022</A>, <A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997322001417/cdio_8k.htm" STYLE="-sec-extract: exhibit">November 10, 2022</A> and <A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997323000326/cdio_8k.htm" STYLE="-sec-extract: exhibit">March 13,
2023</A> (except in each case, for information furnished and not filed under Item 7.01 of Form 8-K); and</FONT></P>

<P STYLE="font: 11pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-indent: 0in"><FONT STYLE="font-size: 9pt; letter-spacing: -0.05pt"></FONT></P>

<P STYLE="font: 11pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-indent: 0in"><FONT STYLE="font-size: 9pt; letter-spacing: -0.05pt"></FONT></P>

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<P STYLE="font: 11pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-indent: 0in"><FONT STYLE="font-size: 9pt; letter-spacing: -0.05pt">(d)</FONT><FONT STYLE="font-family: Times New Roman, Times, Serif; font-size: 7pt; letter-spacing: -0.05pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
</FONT><FONT STYLE="font-size: 10pt">the description of the Registrant&rsquo;s Common Stock contained in the Company&rsquo;s Registration
Statement on Form 8-A, filed with the Commission on November 22, 2021, and any amendment or report filed for the purpose of further updating
such description.</FONT></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">All other documents subsequently filed by the Registrant pursuant
to Section 13(a), 13(c), 14 and 15(d) of the Exchange Act prior to the filing of a post- effective amendment to this Registration Statement
that indicates that all securities offered have been sold or which deregisters all securities then remaining unsold, shall be deemed to
be incorporated by reference in this Registration Statement and to be a part hereof from the date of filing of such documents, except
that information furnished to the Commission under Item 2.02 or Item 7.01 in the Current Reports on Form 8-K and any exhibit relating
to such information, shall not be deemed to be incorporated by reference in this Registration Statement.</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">Any statement contained in a document incorporated or deemed to
be incorporated by reference herein shall be deemed to be modified or superseded for purposes of this Registration Statement to the extent
that a statement contained herein or in any other subsequently filed document that also is or is deemed to be incorporated by reference
herein modifies or supersedes such statement. Any such statement so modified or superseded shall not be deemed, except as so modified
or superseded, to constitute a part of this Registration Statement.</P>

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<TD STYLE="width: 0"></TD><TD STYLE="width: 64.6pt"><B>Item 4.</B></TD><TD><B>Description of Securities.</B></TD></TR></TABLE>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">Not applicable.</P>

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<TD STYLE="width: 0"></TD><TD STYLE="width: 64.6pt"><B>Item 5.</B></TD><TD><B>Interests of Named Experts and Counsel.</B></TD></TR></TABLE>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">Not applicable.</P>

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<TD STYLE="width: 0"></TD><TD STYLE="width: 64.6pt"><B>Item 6.</B></TD><TD><B>Indemnification of Directors and Officers.</B></TD></TR></TABLE>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">Section 145(a) of the General Corporation Law of the State of Delaware,
as amended (&ldquo;DGCL&rdquo;) provides, in general, that a corporation may indemnify any person who was or is a party to or is threatened
to be made a party to any threatened, pending or completed action, suit or proceeding, whether civil, criminal, administrative or investigative
(other than an action by or in the right of the corporation), because he or she is or was a director, officer, employee or agent of the
corporation, or is or was serving at the request of the corporation as a director, officer, employee or agent of another corporation,
partnership, joint venture, trust or other enterprise, against expenses (including attorneys&rsquo; fees), judgments, fines and amounts
paid in settlement actually and reasonably incurred by the person in connection with such action, suit or proceeding, if he or she acted
in good faith and in a manner he or she reasonably believed to be in or not opposed to the best interests of the corporation and, with
respect to any criminal action or proceeding, had no reasonable cause to believe his or her conduct was unlawful.</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">Section 145(b) of the DGCL provides, in general, that a corporation
may indemnify any person who was or is a party or is threatened to be made a party to any threatened, pending or completed action or suit
by or in the right of the corporation to procure a judgment in its favor because the person is or was a director, officer, employee or
agent of the corporation, or is or was serving at the request of the corporation as a director, officer, employee or agent of another
corporation, partnership, joint venture, trust or other enterprise, against expenses (including attorneys&rsquo; fees) actually and reasonably
incurred by the person in connection with the defense or settlement of such action or suit if he or she acted in good faith and in a manner
he or she reasonably believed to be in or not opposed to the best interests of the corporation, except that no indemnification shall be
made with respect to any claim, issue or matter as to which he or she shall have been adjudged to be liable to the corporation unless
and only to the extent that the Court of Chancery or other adjudicating court determines that, despite the adjudication of liability but
in view of all of the circumstances of the case, he or she is fairly and reasonably entitled to indemnity for such expenses that the Court
of Chancery or other adjudicating court shall deem proper.</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">&nbsp;</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt"></P>

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<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">Section 145(g) of the DGCL provides, in general, that a corporation
may purchase and maintain insurance on behalf of any person who is or was a director, officer, employee or agent of the corporation, or
is or was serving at the request of the corporation as a director, officer, employee or agent of another corporation, partnership, joint
venture, trust or other enterprise against any liability asserted against such person and incurred by such person in any such capacity,
or arising out of his or her status as such, whether or not the corporation would have the power to indemnify the person against such
liability under Section 145 of the DGCL. As permitted by the DGCL, the Registrant&rsquo;s amended and restated certificate of incorporation
limits the liability of its directors to the fullest extent permitted by the DGCL providing that a director will not be personally liable
for monetary damages to the Registrant or its stockholders for breach of fiduciary duty as a director to the extent so permitted. The
amended and restated certificate of incorporation also provides that each current or former director, officer, employee or agent of the
Registrant, or each such person who is or was serving or who had agreed to serve at the request of the Registrant as a director, officer,
employee or agent of another corporation, partnership, joint venture, trust or any other enterprise will be indemnified by the Registrant
to the full extent permitted by law, as the same exists or may in the future be amended. The Registrant&rsquo;s bylaws provide that it
will indemnify, and provide expense advancement to, its directors and officers to the fullest extent permitted by such law, subject to
certain exceptions contained in the Registrant&rsquo;s amended and restated bylaws.</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">Pursuant to Section 102(b)(7) of the DGCL, the Registrant&rsquo;s
Amended and Restated Certificate of Incorporation eliminates the liability of a director to the Registrant or its stockholders for monetary
damages for breach of fiduciary duty as a director to the fullest extent permitted by the DGCL, as may be amended from time to time.</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">The Registrant has approved and intends to enter into indemnification
agreements with each of its directors and executive officers. These agreements provide that the Registrant will indemnify, and provide
expense advancement and reimbursement to, each of its directors and such officers to the fullest extent permitted by law and its amended
and restated certificate of incorporation and its bylaws, subject to certain exceptions contained in those agreements. Any claims for
indemnification by the Registrant&rsquo;s directors and officers may reduce the Registrant&rsquo;s available funds to satisfy successful
third-party claims against it and may reduce the amount of money available to the Registrant. The Registrant also maintains a general
liability insurance policy, which will cover certain liabilities of directors and officers of the Registrant arising out of claims based
on acts or omissions in their capacities as directors or officers.</P>

<TABLE CELLPADDING="0" CELLSPACING="0" WIDTH="100%" STYLE="font: 10pt Times New Roman, Times, Serif; margin-top: 0; margin-bottom: 12pt"><TR STYLE="vertical-align: top">
<TD STYLE="width: 0"></TD><TD STYLE="width: 64.6pt"><B>Item 7.</B></TD><TD><B>Exemption from Registration Claimed.</B></TD></TR></TABLE>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">Not <FONT STYLE="letter-spacing: -0.1pt">applicable.</FONT></P>

<TABLE CELLPADDING="0" CELLSPACING="0" WIDTH="100%" STYLE="font: 10pt Times New Roman, Times, Serif; margin-top: 0; margin-bottom: 12pt"><TR STYLE="vertical-align: top">
<TD STYLE="width: 0"></TD><TD STYLE="width: 64.6pt"><B>Item 8.</B></TD><TD><B>Exhibits.</B></TD></TR></TABLE>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">The following exhibits are filed as part of this Registration <FONT STYLE="letter-spacing: -0.1pt">Statement:</FONT></P>

<TABLE CELLSPACING="0" CELLPADDING="0" STYLE="width: 100%; font: 12pt Times New Roman, Times, Serif; border-collapse: collapse">
  <TR STYLE="vertical-align: top">
    <TD STYLE="width: 10%; border-bottom: Black 1pt solid; font-size: 9pt; text-align: center"><FONT STYLE="font-size: 10pt"><B>Exhibit<BR>
Number</B></FONT></TD>
    <TD STYLE="width: 2%">
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-align: center"><B>&nbsp;</B></P>
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0"><B>&nbsp;</B></P></TD>
    <TD STYLE="width: 88%; border-bottom: Black 1pt solid">
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-align: center"><B>&nbsp;</B></P>
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-align: center"><B>Description of Exhibit</B></P></TD></TR>
  <TR STYLE="vertical-align: top">
    <TD STYLE="font-size: 9pt; text-align: right">&nbsp;</TD>
    <TD STYLE="font-size: 9pt">&nbsp;</TD>
    <TD STYLE="font-size: 9pt">&nbsp;</TD></TR>
  <TR STYLE="vertical-align: top">
    <TD STYLE="font-size: 9pt; text-align: right"><FONT STYLE="font-size: 10pt">3.1&nbsp;</FONT></TD>
    <TD STYLE="font-size: 9pt">&nbsp;</TD>
    <TD STYLE="font-size: 9pt"><FONT STYLE="font-size: 10pt"><A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997322001382/ex3x1.htm" STYLE="-sec-extract: exhibit">Second Amended and Restated Certificate of Incorporation of Cardio Diagnostics Holdings, Inc.</A> (incorporated by reference to Exhibit 3.1 of the Registrant&rsquo;s Current Report on Form 8-K, filed with the Commission on October 31, 2022)</FONT></TD></TR>
  <TR STYLE="vertical-align: top">
    <TD STYLE="font-size: 9pt; text-align: right"><FONT STYLE="font-size: 10pt">3.2&nbsp;</FONT></TD>
    <TD STYLE="font-size: 9pt">&nbsp;</TD>
    <TD STYLE="font-size: 9pt"><FONT STYLE="font-size: 10pt"><A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997321001049/ex3x3.htm" STYLE="-sec-extract: exhibit">Bylaws of Cardio Diagnostics Holdings Inc.</A> (incorporated by reference to Exhibit 3.3 of the Registrant&rsquo;s Registration Statement on Form S-1, filed
with the Commission on October 19, 2021)</FONT></TD></TR>
  <TR STYLE="vertical-align: top">
    <TD STYLE="font-size: 9pt; text-align: right"><FONT STYLE="font-size: 10pt">5.1*</FONT></TD>
    <TD STYLE="font-size: 9pt">&nbsp;</TD>
    <TD STYLE="font-size: 9pt"><FONT STYLE="font-size: 10pt"><A HREF="ex5x1.htm">Opinion of Shartsis Friese LLP</A></FONT></TD></TR>
  <TR STYLE="vertical-align: top">
    <TD STYLE="font-size: 9pt; text-align: right"><FONT STYLE="font-size: 10pt">23.1*</FONT></TD>
    <TD STYLE="font-size: 9pt">&nbsp;</TD>
    <TD STYLE="font-size: 9pt"><FONT STYLE="font-size: 10pt"><A HREF="ex23x1.htm">Consent of MaloneBailey LLP</A></FONT></TD></TR>
  <TR STYLE="vertical-align: top">
    <TD STYLE="font-size: 9pt; text-align: right"><FONT STYLE="font-size: 10pt">23.2*</FONT></TD>
    <TD STYLE="font-size: 9pt">&nbsp;</TD>
    <TD STYLE="font-size: 9pt"><FONT STYLE="font-size: 10pt"><A HREF="ex23x2.htm">Consent of Prager Metis CPA&rsquo;s LLC</A></FONT></TD></TR>
  <TR STYLE="vertical-align: top">
    <TD STYLE="font-size: 9pt; text-align: right"><FONT STYLE="font-size: 10pt">23.3*</FONT></TD>
    <TD STYLE="font-size: 9pt">&nbsp;</TD>
    <TD STYLE="font-size: 9pt"><FONT STYLE="font-size: 10pt"><A HREF="ex5x1.htm">Consent of Shartsis Friese LLP</A> (included in Exhibit 5.1)</FONT></TD></TR>
  <TR STYLE="vertical-align: top">
    <TD STYLE="font-size: 9pt; text-align: right"><FONT STYLE="font-size: 10pt">24.1*</FONT></TD>
    <TD STYLE="font-size: 9pt">&nbsp;</TD>
    <TD STYLE="font-size: 9pt"><FONT STYLE="font-size: 10pt"><A HREF="#a_001">Power of Attorney</A> (included on the signature page of this Registration Statement)</FONT></TD></TR>
  <TR STYLE="vertical-align: top">
    <TD STYLE="font-size: 9pt; text-align: right"><FONT STYLE="font-size: 10pt">99.1&nbsp;</FONT></TD>
    <TD STYLE="font-size: 9pt">&nbsp;</TD>
    <TD STYLE="font-size: 9pt"><FONT STYLE="font-size: 10pt"><A HREF="http://www.sec.gov/Archives/edgar/data/1870144/000107997322001382/ex10x4.htm" STYLE="-sec-extract: exhibit">Cardio Diagnostics Holdings, Inc. 2022 Equity Incentive Plan</A> (incorporated by reference to Exhibit 10.4 of the Registrant&rsquo;s Current Report on Form 8-K, filed with the Commission on October 31, 2022)</FONT></TD></TR>
  <TR STYLE="vertical-align: top">
    <TD STYLE="font-size: 9pt; text-align: right"><FONT STYLE="font-size: 10pt">107*</FONT></TD>
    <TD STYLE="font-size: 9pt">&nbsp;</TD>
    <TD STYLE="font-size: 9pt"><FONT STYLE="font-size: 10pt"><A HREF="ex107.htm">Filing Fee Table</A></FONT></TD></TR>
  <TR STYLE="vertical-align: top">
    <TD STYLE="font-size: 9pt; text-align: right">&nbsp;</TD>
    <TD STYLE="font-size: 9pt">&nbsp;</TD>
    <TD STYLE="font-size: 9pt">&nbsp;</TD></TR>
  </TABLE>
<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 0 0.1in">__________</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 0 0.1in">* Filed <FONT STYLE="letter-spacing: -0.1pt">herewith</FONT></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt 0.1in">&nbsp;</P>

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<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt 0.1in"></P>

<TABLE CELLPADDING="0" CELLSPACING="0" WIDTH="100%" STYLE="font: 10pt Times New Roman, Times, Serif; margin-top: 0; margin-bottom: 12pt"><TR STYLE="vertical-align: top">
<TD STYLE="width: 0"></TD><TD STYLE="width: 64.6pt"><B>Item <FONT STYLE="letter-spacing: -0.25pt">9.</FONT></B></TD><TD><FONT STYLE="letter-spacing: -0.1pt"><B>Undertakings.</B></FONT></TD></TR></TABLE>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">1.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The undersigned Registrant
hereby undertakes:</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-indent: 0.5in">(a)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;To
file, during any period in which offers or sales are being made, a post-effective amendment to the Registration Statement:</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt 1in">(i)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;to include any
prospectus required by Section 10(a)(3) of the Securities Act&#894;</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-indent: 1in">(ii)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;to
reflect in the prospectus any facts or events arising after the effective date of the Registration Statement (or the most recent post-effective
amendment thereof), which individually or in the aggregate, represent a fundamental change in the information set forth in the Registration
Statement. Notwithstanding the foregoing, any increase or decrease in volume of securities offered (if the total dollar value of securities
offered would not exceed that which was registered) and any deviation from the low or high end of the estimated maximum offering range
may be reflected in the form of prospectus filed with the Commission pursuant to Rule 424(b) if, in the aggregate, the changes in volume
and price represent no more than a 20% change in the maximum aggregate offering price set forth in the &ldquo;Calculation of Registration
Fee&rdquo; table in the effective Registration Statement&#894; and</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-indent: 1in">(iii)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;to
include any material information with respect to the plan of distribution not previously disclosed in the Registration Statement or any
material change to such information in the Registration Statement;</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">provided, however, that paragraphs (1)(a)(i) and (1)(a)(ii) do not
apply if the information required to be included in a post-effective amendment by those paragraphs is contained in periodic reports filed
with, or furnished to, the Commission by the Registrant pursuant to Section 13 or Section 15(d) of the Exchange Act that are incorporated
by reference in the Registration Statement.</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-indent: 0.5in">(b)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;That,
for the purpose of determining any liability under the Securities Act, each such post-effective amendment shall be deemed to be a new
registration statement relating to the securities offered therein, and the offering of such securities at that time shall be deemed to
be the initial bona fide offering thereof.</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-indent: 0.5in">(c)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;To
remove from registration by means of a post-effective amendment any of the securities being registered which remain unsold at the termination
of the offering.</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">2.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The undersigned registrant
hereby undertakes that, for purposes of determining any liability under the Securities Act, each filing of the Registrant&rsquo;s annual
report pursuant to Section 13(a) or Section 15(d) of the Exchange Act (and, where applicable, each filing of an employee benefit plan&rsquo;s
annual report pursuant to Section 15(d) of the Exchange Act) that is incorporated by reference in the Registration Statement shall be
deemed to be a new registration statement relating to the securities offered therein, and the offering of such securities at that time
shall be deemed to be the initial bona fide offering thereof.</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">3.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Insofar as indemnification
for liabilities arising under the Securities Act may be permitted to directors, officers and controlling persons of the Registrant pursuant
to the foregoing provisions, or otherwise, the Registrant has been advised that in the opinion of the Commission such indemnification
is against public policy as expressed in the Securities Act and is, therefore, unenforceable. In the event that a claim for indemnification
against such liabilities (other than the payment by the Registrant of expenses incurred or paid by a director, officer or controlling
person of the Registrant in the successful defense of any action, suit or proceeding) is asserted by such director, officer or controlling
person in connection with the securities being registered, the Registrant will, unless in the opinion of its counsel the matter has been
settled by controlling precedent, submit to a court of appropriate jurisdiction the question whether such indemnification by it is against
public policy as expressed in the Securities Act and will be governed by the final adjudication of such issue.</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">&nbsp;</P>


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<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-align: center"><B>SIGNATURES</B></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">Pursuant to the requirements of the Securities Act of 1933, as
amended, the registrant has duly caused this registration statement to be signed on its behalf by the undersigned, thereunto duly authorized,
in the City of Chicago, Illinois, on the 22nd day of March, 2023.<B>&nbsp;</B></P>

<TABLE BORDER="0" CELLSPACING="0" CELLPADDING="0" STYLE="width: 100%; font: 10pt Times New Roman, Times, Serif">
<TR>
    <TD STYLE="vertical-align: top">&nbsp;</TD>
    <TD COLSPAN="2"><B>CARDIO DIAGNOSTICS HOLDINGS, INC.</B></TD>
</TR>
<TR>
    <TD STYLE="vertical-align: top">&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD></TR>
<TR>
    <TD STYLE="vertical-align: top; width: 50%">&nbsp;</TD>
    <TD STYLE="width: 4%">&nbsp;By:</TD>
    <TD STYLE="vertical-align: top; width: 46%">/s/ Meeshanthini V. Dogan</TD>
</TR>
<TR>
    <TD STYLE="vertical-align: top">&nbsp;</TD>
    <TD>&nbsp;</TD>
    <TD STYLE="vertical-align: top; border-top: #000000 1px solid">Meeshanthini V. Dogan<BR><I>Chief Executive Officer</I></TD>
</TR>
</TABLE>

<P STYLE="font: 10pt Times New Roman, Times, Serif; text-align: left; text-indent: 0pt; margin-top: 0pt; margin-bottom: 6pt">&nbsp;</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt"></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt; text-align: center"><A NAME="a_001"></A><B>POWER OF ATTORNEY</B></P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">KNOW ALL MEN BY THESE PRESENTS, that of the undersigned constitutes
and appoints Meeshanthini V. Dogan and Elisa Luqman, and each of them, her true and&nbsp;lawful&nbsp;attorney-in-fact&nbsp;and&nbsp;agent,
with full power of substitution and revocation, for her and in her name, place and stead, in any and all capacities, to execute any or
all amendments including any post-effective amendments and supplements to this Registration Statement, and any additional Registration
Statement filed pursuant to Rule&nbsp;462(b), and to file the same, with all exhibits thereto, and other documents in connection therewith,
with the Securities and Exchange Commission, granting unto&nbsp;said&nbsp;attorney-in-fact&nbsp;and&nbsp;agent full power and authority
to do and perform each and every act and thing requisite and necessary to be done, as fully to all intents and purposes as she might or
could do in person, hereby ratifying and confirming all that&nbsp;said&nbsp;attorney-in-fact&nbsp;and&nbsp;agent, or her substitute or
substitutes, may lawfully do or cause to be done by virtue hereof.</P>

<P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 12pt">Pursuant to the requirements of the Securities Act of 1933, as amended,
this registration statement has been signed below by the following persons in the capacities and on the dates indicated.</P>

<TABLE CELLSPACING="0" CELLPADDING="0" STYLE="width: 100%; border-collapse: collapse; font-family: Times New Roman, Times, Serif">
  <TR>
    <TD STYLE="width: 35%">&nbsp;</TD>
    <TD STYLE="vertical-align: bottom; width: 1%">&nbsp;</TD>
    <TD STYLE="width: 43%">&nbsp;</TD>
    <TD STYLE="vertical-align: bottom; width: 2%">&nbsp;</TD>
    <TD STYLE="width: 19%">&nbsp;</TD></TR>
  <TR STYLE="vertical-align: bottom">
    <TD STYLE="font-size: 12pt; text-align: center"><FONT STYLE="font-size: 10pt"><B><U>Name</U></B></FONT></TD>
    <TD STYLE="font-size: 12pt">&nbsp;</TD>
    <TD STYLE="font-size: 12pt; text-align: center"><FONT STYLE="font-size: 10pt"><B><U>Position</U></B></FONT></TD>
    <TD STYLE="font-size: 12pt">&nbsp;</TD>
    <TD STYLE="font-size: 12pt; text-align: center"><FONT STYLE="font-size: 10pt"><B><U>Date</U></B></FONT></TD></TR>
  <TR>
    <TD>&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD></TR>
  <TR>
    <TD STYLE="vertical-align: top">
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-indent: 0.25in; border-bottom: black 0.75pt solid">/s/ Meeshanthini
    V. Dogan</P>
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 1pt">Meeshanthini V. Dogan</P></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="vertical-align: top">
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-align: center">Chief Executive Officer, Director</P>
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-align: center">(principal executive officer)</P></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="white-space: nowrap; vertical-align: top; font-size: 12pt"><FONT STYLE="font-size: 10pt">March 22, 2023</FONT></TD></TR>
  <TR>
    <TD>&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD></TR>
  <TR>
    <TD STYLE="vertical-align: top">
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-indent: 0.25in; border-bottom: black 0.75pt solid">/s/ Elisa Luqman</P>
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 1pt">Elisa Luqman</P></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="vertical-align: top">
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-align: center">Chief Financial Officer</P>
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-align: center">(principal financial officer and principal accounting
    officer)</P></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="white-space: nowrap; vertical-align: top; font-size: 12pt"><FONT STYLE="font-size: 10pt">March 22, 2023</FONT></TD></TR>
  <TR>
    <TD>&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD></TR>
  <TR>
    <TD STYLE="vertical-align: top">
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-indent: 0.25in; border-bottom: black 0.75pt solid">/s/ Warren Hosseinion</P></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="vertical-align: top; font-size: 12pt; text-align: center"><FONT STYLE="font-size: 10pt">Director, Chair</FONT></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="white-space: nowrap; vertical-align: top; font-size: 12pt"><FONT STYLE="font-size: 10pt">March 22, 2023</FONT></TD></TR>
  <TR>
    <TD STYLE="font-size: 12pt"><FONT STYLE="font-size: 10pt">Warren Hosseinion</FONT></TD>
    <TD COLSPAN="2">&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD></TR>
  <TR>
    <TD STYLE="font-size: 12pt">&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD></TR>
  <TR>
    <TD STYLE="vertical-align: top">
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-indent: 0.25in; border-bottom: black 0.75pt solid">/s/ James Intrater</P>
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 1pt">James Intrater</P></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="vertical-align: top; font-size: 12pt; text-align: center"><FONT STYLE="font-size: 10pt">Director</FONT></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="white-space: nowrap; vertical-align: top; font-size: 12pt"><FONT STYLE="font-size: 10pt">March 22, 2023</FONT></TD></TR>
  <TR>
    <TD>&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD></TR>
  <TR>
    <TD STYLE="vertical-align: top">
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-indent: 0.25in; border-bottom: black 0.75pt solid">/s/ Stanley M.
    Lau</P>
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 1pt">Stanley M. Lau</P></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="vertical-align: top; font-size: 12pt; text-align: center"><FONT STYLE="font-size: 10pt">Director</FONT></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="white-space: nowrap; vertical-align: top; font-size: 12pt"><FONT STYLE="font-size: 10pt">March 22, 2023</FONT></TD></TR>
  <TR>
    <TD>&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD></TR>
  <TR>
    <TD STYLE="vertical-align: top">
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-indent: 0.25in; border-bottom: black 0.75pt solid">/s/ Oded Levy</P>
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0 0 1pt">Oded Levy</P></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="vertical-align: top; font-size: 12pt; text-align: center"><FONT STYLE="font-size: 10pt">Director</FONT></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="white-space: nowrap; vertical-align: top; font-size: 12pt"><FONT STYLE="font-size: 10pt">March 22, 2023</FONT></TD></TR>
  <TR>
    <TD>&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD>
    <TD COLSPAN="2">&nbsp;</TD></TR>
  <TR>
    <TD STYLE="vertical-align: top">
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-indent: 0.25in; border-bottom: black 0.75pt solid">/s/ Robert Philibert</P>
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0">Robert Philibert</P>
    <P STYLE="font: 12pt Times New Roman, Times, Serif; margin: 0 0 1pt">&nbsp;</P></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="vertical-align: top; font-size: 12pt; text-align: center"><FONT STYLE="font-size: 10pt">Director</FONT></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="white-space: nowrap; vertical-align: top; font-size: 12pt"><FONT STYLE="font-size: 10pt">March 22, 2023</FONT></TD></TR>
  <TR>
    <TD STYLE="vertical-align: top">
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0; text-indent: 0.25in; border-bottom: black 0.75pt solid">/s/ Brandon Sim</P></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="vertical-align: top; font-size: 12pt; text-align: center"><FONT STYLE="font-size: 10pt">Director</FONT></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="white-space: nowrap; vertical-align: top; font-size: 12pt"><FONT STYLE="font-size: 10pt">March 22, 2023</FONT></TD></TR>
  <TR>
    <TD STYLE="vertical-align: top">
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0">Brandon Sim</P>
    <P STYLE="font: 10pt Times New Roman, Times, Serif; margin: 0">&nbsp;</P></TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="vertical-align: top; font-size: 12pt; text-align: center">&nbsp;</TD>
    <TD STYLE="vertical-align: bottom; font-size: 12pt">&nbsp;</TD>
    <TD STYLE="white-space: nowrap; vertical-align: top; font-size: 12pt">&nbsp;</TD></TR>
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[{"type": "S-8", "sequence": "1", "filename": "cdio_s8.htm", "description": "FORM S-8", "secsgml_size_bytes": 51369}]
{"company-data": {"conformed-name": "Cardio Diagnostics Holdings, Inc.", "cik": "0001870144", "assigned-sic": "2835", "irs-number": "870925574", "fiscal-year-end": "1231"}, "filing-values": {"form-type": "S-8", "act": "33", "file-number": "333-270752", "film-number": "23753374"}, "business-address": {"street1": "400 N ABERDEEN ST, SUITE 900", "city": "CHICAGO", "state": "IL", "zip": "60642", "phone": "855-226-9991"}, "mail-address": {"street1": "400 N ABERDEEN ST, SUITE 900", "city": "CHICAGO", "state": "IL", "zip": "60642"}, "former-company": {"former-conformed-name": "Mana Capital Acquisition Corp.", "date-changed": "20210629"}}
As filed with the U.S. Securities and Exchange Commission on March 22, 2023.
Registration No. 333-_______
UNITED STATES
SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549
____________________________
FORM S-8
REGISTRATION STATEMENT
UNDER
THE SECURITIES ACT OF 1933
____________________________
CARDIO DIAGNOSTICS
HOLDINGS, INC.
(Exact name of registrant as specified in its charter)
Delaware
6770
87-0925574
(State or other jurisdiction of
incorporation or organization)
(Primary Standard Industrial
Classification Code Number)
(I.R.S. Employer
Identification No.)
400 North Aberdeen Street, Suite 900
Chicago, IL 60642
Telephone: (855) 226-9991
(Address, including zip code, and telephone number, including area code, of registrant's principal executive offices)
Cardio Diagnostics Holdings, Inc. 2022 Equity Incentive
Plan
(Full Title of the Plan)
____________________________
Meeshanthini V. Dogan, Ph.D.
Chief Executive Officer
Cardio Diagnostics Holdings, Inc.
400 North Aberdeen Street, Suite 900
Chicago, IL 60642
Telephone: (855) 226-9991
(Name, address, including zip code, and telephone number, including area code, of agent for service)
____________________________
Copies to:
P. Rupert Russell, Esq.
Shartsis Friese LLP
One Maritime Plaza, 18th Floor
San Francisco, CA 94111
(415) 421-6500
Indicate by check mark whether the registrant is
a large accelerated filer, an accelerated filer, a non-accelerated filer, smaller reporting company, or an emerging growth company.
See the definitions of "large accelerated filer," "accelerated filer," "smaller reporting company,"
and "emerging growth company" in Rule 12b-2 of the Exchange Act.
Large accelerated filer
☐
Accelerated filer
☐
Non- accelerated filer
☒
Smaller reporting company
☒
Emerging growth company
☒
If an emerging growth company, indicate by check
mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting
standards provided pursuant to Section 7(a)(2)(B) of the Securities Act.
☐
EXPLANATORY NOTE
This Registration Statement on Form S-8 is being filed by Cardio
Diagnostics Holdings, Inc. (the "
Company
" and the "
Registrant
") for the purpose of
registering 3,265,516 shares of the Company's common stock, par value of $0.00001 per share, issuable under the Cardio Diagnostics
Holdings, Inc. 2022 Equity Incentive Plan (the "
2022 Plan
").
On October 25, 2022, pursuant to that certain Business Combination
Agreement, dated as of May 27, 2022 as amended by Amendment No. 1 to the Agreement, dated September 15, 2022 (the "
Business
Combination Agreement
"), by and among Mana Capital Acquisition Corp., a Delaware corporation ("
Mana
"),
Mana Merger Sub, Inc., a Delaware corporation and wholly-owned subsidiary of Mana ("
Merger Sub
"), Cardio Diagnostics,
Inc., a Delaware corporation ("
Legacy Cardio
"), and
Meeshanthini
Dogan, PhD, as the "
Shareholders' Representative
,"
Merger Sub merged with and into Legacy Cardio,
with Legacy Cardio continuing as the surviving corporation, resulting in Legacy Cardio becoming a wholly-owned subsidiary of the Company.
The Registrant's Common Stock commenced trading on the Nasdaq Capital Market under the symbol "CDIO" on October 26,
2022.
PART I
INFORMATION REQUIRED IN THE SECTION 10(a) PROSPECTUS
The documents containing the information specified in Items 1 and
2 of this Part I of Form S-8 (plan information and registration information and employee plan annual information) will be sent or given
to employees as specified by the Securities and Exchange Commission (the "
Commission
") pursuant to Rule 428(b)(1)
of the Securities Act. Such documents are not required to be and are not filed with the Commission either as part of this registration
statement (this "
Registration Statement
") or as prospectuses or prospectus supplements pursuant to Rule 424.
These documents and the documents incorporated by reference in this Registration Statement pursuant to Item 3 of Part II hereof, taken
together, constitute a prospectus that meets the requirements of Section 10(a) of the Securities Act. The Company will provide a written
statement to participants advising them of the availability without charge, upon written or oral request, of the documents incorporated
by reference in Item 3 of Part II hereof and including the statement in the preceding sentence. The written statement to all participants
will indicate the availability without charge, upon written or oral request, of other documents required to be delivered pursuant to Rule
428(b) and will include the address and telephone number to which the request is to be directed.
PART II
INFORMATION REQUIRED IN THE REGISTRATION STATEMENT
Item 3.
Incorporation of Documents by Reference.
The following documents filed by Cardio Diagnostics Holdings, Inc.,
or its predecessor, Mana Capital Acquisition Corp., with the Commission pursuant to the Securities Exchange Act of 1934, as amended, (the
"Exchange Act") are incorporated by reference in, and shall be deemed to be a part of, this Registration
Statement:
(a)
the Registrant's Annual Report on
Form 10-K
for the fiscal year ended December 31, 2021, filed
with the Commission on March 31, 2022, which Report contains Mana's audited financial statements for the latest fiscal year for
which such statements have been filed;
(b)
the Registrant's Quarterly Reports on Form 10-Q for the fiscal quarters ended
March 31, 2022
,
June 30, 2022
and
September 30, 2022
, filed with the Commission on May 12, 2022, August 10, 2022 and November 7, 2022, respectively;
(c)
the Registrant's Current Reports on Form 8-K filed with the Commission on
May 31, 2022
,
August
25, 2022
,
August 26, 2022
,
September 15, 2022
,
September 21, 2022
,
September 23, 2022
,
October 31, 2022
,
November 10, 2022
and
March 13,
2023
(except in each case, for information furnished and not filed under Item 7.01 of Form 8-K); and
1
(d)
the description of the Registrant's Common Stock contained in the Company's Registration
Statement on Form 8-A, filed with the Commission on November 22, 2021, and any amendment or report filed for the purpose of further updating
such description.
All other documents subsequently filed by the Registrant pursuant
to Section 13(a), 13(c), 14 and 15(d) of the Exchange Act prior to the filing of a post- effective amendment to this Registration Statement
that indicates that all securities offered have been sold or which deregisters all securities then remaining unsold, shall be deemed to
be incorporated by reference in this Registration Statement and to be a part hereof from the date of filing of such documents, except
that information furnished to the Commission under Item 2.02 or Item 7.01 in the Current Reports on Form 8-K and any exhibit relating
to such information, shall not be deemed to be incorporated by reference in this Registration Statement.
Any statement contained in a document incorporated or deemed to
be incorporated by reference herein shall be deemed to be modified or superseded for purposes of this Registration Statement to the extent
that a statement contained herein or in any other subsequently filed document that also is or is deemed to be incorporated by reference
herein modifies or supersedes such statement. Any such statement so modified or superseded shall not be deemed, except as so modified
or superseded, to constitute a part of this Registration Statement.
Item 4.
Description of Securities.
Not applicable.
Item 5.
Interests of Named Experts and Counsel.
Not applicable.
Item 6.
Indemnification of Directors and Officers.
Section 145(a) of the General Corporation Law of the State of Delaware,
as amended ("DGCL") provides, in general, that a corporation may indemnify any person who was or is a party to or is threatened
to be made a party to any threatened, pending or completed action, suit or proceeding, whether civil, criminal, administrative or investigative
(other than an action by or in the right of the corporation), because he or she is or was a director, officer, employee or agent of the
corporation, or is or was serving at the request of the corporation as a director, officer, employee or agent of another corporation,
partnership, joint venture, trust or other enterprise, against expenses (including attorneys' fees), judgments, fines and amounts
paid in settlement actually and reasonably incurred by the person in connection with such action, suit or proceeding, if he or she acted
in good faith and in a manner he or she reasonably believed to be in or not opposed to the best interests of the corporation and, with
respect to any criminal action or proceeding, had no reasonable cause to believe his or her conduct was unlawful.
Section 145(b) of the DGCL provides, in general, that a corporation
may indemnify any person who was or is a party or is threatened to be made a party to any threatened, pending or completed action or suit
by or in the right of the corporation to procure a judgment in its favor because the person is or was a director, officer, employee or
agent of the corporation, or is or was serving at the request of the corporation as a director, officer, employee or agent of another
corporation, partnership, joint venture, trust or other enterprise, against expenses (including attorneys' fees) actually and reasonably
incurred by the person in connection with the defense or settlement of such action or suit if he or she acted in good faith and in a manner
he or she reasonably believed to be in or not opposed to the best interests of the corporation, except that no indemnification shall be
made with respect to any claim, issue or matter as to which he or she shall have been adjudged to be liable to the corporation unless
and only to the extent that the Court of Chancery or other adjudicating court determines that, despite the adjudication of liability but
in view of all of the circumstances of the case, he or she is fairly and reasonably entitled to indemnity for such expenses that the Court
of Chancery or other adjudicating court shall deem proper.
2
Section 145(g) of the DGCL provides, in general, that a corporation
may purchase and maintain insurance on behalf of any person who is or was a director, officer, employee or agent of the corporation, or
is or was serving at the request of the corporation as a director, officer, employee or agent of another corporation, partnership, joint
venture, trust or other enterprise against any liability asserted against such person and incurred by such person in any such capacity,
or arising out of his or her status as such, whether or not the corporation would have the power to indemnify the person against such
liability under Section 145 of the DGCL. As permitted by the DGCL, the Registrant's amended and restated certificate of incorporation
limits the liability of its directors to the fullest extent permitted by the DGCL providing that a director will not be personally liable
for monetary damages to the Registrant or its stockholders for breach of fiduciary duty as a director to the extent so permitted. The
amended and restated certificate of incorporation also provides that each current or former director, officer, employee or agent of the
Registrant, or each such person who is or was serving or who had agreed to serve at the request of the Registrant as a director, officer,
employee or agent of another corporation, partnership, joint venture, trust or any other enterprise will be indemnified by the Registrant
to the full extent permitted by law, as the same exists or may in the future be amended. The Registrant's bylaws provide that it
will indemnify, and provide expense advancement to, its directors and officers to the fullest extent permitted by such law, subject to
certain exceptions contained in the Registrant's amended and restated bylaws.
Pursuant to Section 102(b)(7) of the DGCL, the Registrant's
Amended and Restated Certificate of Incorporation eliminates the liability of a director to the Registrant or its stockholders for monetary
damages for breach of fiduciary duty as a director to the fullest extent permitted by the DGCL, as may be amended from time to time.
The Registrant has approved and intends to enter into indemnification
agreements with each of its directors and executive officers. These agreements provide that the Registrant will indemnify, and provide
expense advancement and reimbursement to, each of its directors and such officers to the fullest extent permitted by law and its amended
and restated certificate of incorporation and its bylaws, subject to certain exceptions contained in those agreements. Any claims for
indemnification by the Registrant's directors and officers may reduce the Registrant's available funds to satisfy successful
third-party claims against it and may reduce the amount of money available to the Registrant. The Registrant also maintains a general
liability insurance policy, which will cover certain liabilities of directors and officers of the Registrant arising out of claims based
on acts or omissions in their capacities as directors or officers.
Item 7.
Exemption from Registration Claimed.
Not
applicable.
Item 8.
Exhibits.
The following exhibits are filed as part of this Registration
Statement:
Exhibit
Number
Description of Exhibit
3.1
Second Amended and Restated Certificate of Incorporation of Cardio Diagnostics Holdings, Inc.
(incorporated by reference to Exhibit 3.1 of the Registrant's Current Report on Form 8-K, filed with the Commission on October 31, 2022)
3.2
Bylaws of Cardio Diagnostics Holdings Inc.
(incorporated by reference to Exhibit 3.3 of the Registrant's Registration Statement on Form S-1, filed
with the Commission on October 19, 2021)
5.1*
Opinion of Shartsis Friese LLP
23.1*
Consent of MaloneBailey LLP
23.2*
Consent of Prager Metis CPA's LLC
23.3*
Consent of Shartsis Friese LLP
(included in Exhibit 5.1)
24.1*
Power of Attorney
(included on the signature page of this Registration Statement)
99.1
Cardio Diagnostics Holdings, Inc. 2022 Equity Incentive Plan
(incorporated by reference to Exhibit 10.4 of the Registrant's Current Report on Form 8-K, filed with the Commission on October 31, 2022)
107*
Filing Fee Table
__________
* Filed
herewith
3
Item
9.
Undertakings.
1.       The undersigned Registrant
hereby undertakes:
(a)       To
file, during any period in which offers or sales are being made, a post-effective amendment to the Registration Statement:
(i)       to include any
prospectus required by Section 10(a)(3) of the Securities Act;
(ii)       to
reflect in the prospectus any facts or events arising after the effective date of the Registration Statement (or the most recent post-effective
amendment thereof), which individually or in the aggregate, represent a fundamental change in the information set forth in the Registration
Statement. Notwithstanding the foregoing, any increase or decrease in volume of securities offered (if the total dollar value of securities
offered would not exceed that which was registered) and any deviation from the low or high end of the estimated maximum offering range
may be reflected in the form of prospectus filed with the Commission pursuant to Rule 424(b) if, in the aggregate, the changes in volume
and price represent no more than a 20% change in the maximum aggregate offering price set forth in the "Calculation of Registration
Fee" table in the effective Registration Statement; and
(iii)       to
include any material information with respect to the plan of distribution not previously disclosed in the Registration Statement or any
material change to such information in the Registration Statement;
provided, however, that paragraphs (1)(a)(i) and (1)(a)(ii) do not
apply if the information required to be included in a post-effective amendment by those paragraphs is contained in periodic reports filed
with, or furnished to, the Commission by the Registrant pursuant to Section 13 or Section 15(d) of the Exchange Act that are incorporated
by reference in the Registration Statement.
(b)       That,
for the purpose of determining any liability under the Securities Act, each such post-effective amendment shall be deemed to be a new
registration statement relating to the securities offered therein, and the offering of such securities at that time shall be deemed to
be the initial bona fide offering thereof.
(c)       To
remove from registration by means of a post-effective amendment any of the securities being registered which remain unsold at the termination
of the offering.
2.       The undersigned registrant
hereby undertakes that, for purposes of determining any liability under the Securities Act, each filing of the Registrant's annual
report pursuant to Section 13(a) or Section 15(d) of the Exchange Act (and, where applicable, each filing of an employee benefit plan's
annual report pursuant to Section 15(d) of the Exchange Act) that is incorporated by reference in the Registration Statement shall be
deemed to be a new registration statement relating to the securities offered therein, and the offering of such securities at that time
shall be deemed to be the initial bona fide offering thereof.
3.       Insofar as indemnification
for liabilities arising under the Securities Act may be permitted to directors, officers and controlling persons of the Registrant pursuant
to the foregoing provisions, or otherwise, the Registrant has been advised that in the opinion of the Commission such indemnification
is against public policy as expressed in the Securities Act and is, therefore, unenforceable. In the event that a claim for indemnification
against such liabilities (other than the payment by the Registrant of expenses incurred or paid by a director, officer or controlling
person of the Registrant in the successful defense of any action, suit or proceeding) is asserted by such director, officer or controlling
person in connection with the securities being registered, the Registrant will, unless in the opinion of its counsel the matter has been
settled by controlling precedent, submit to a court of appropriate jurisdiction the question whether such indemnification by it is against
public policy as expressed in the Securities Act and will be governed by the final adjudication of such issue.
4
SIGNATURES
Pursuant to the requirements of the Securities Act of 1933, as
amended, the registrant has duly caused this registration statement to be signed on its behalf by the undersigned, thereunto duly authorized,
in the City of Chicago, Illinois, on the 22nd day of March, 2023.
CARDIO DIAGNOSTICS HOLDINGS, INC.
By:
/s/ Meeshanthini V. Dogan
Meeshanthini V. Dogan
Chief Executive Officer
POWER OF ATTORNEY
KNOW ALL MEN BY THESE PRESENTS, that of the undersigned constitutes
and appoints Meeshanthini V. Dogan and Elisa Luqman, and each of them, her true and lawful attorney-in-fact and agent,
with full power of substitution and revocation, for her and in her name, place and stead, in any and all capacities, to execute any or
all amendments including any post-effective amendments and supplements to this Registration Statement, and any additional Registration
Statement filed pursuant to Rule 462(b), and to file the same, with all exhibits thereto, and other documents in connection therewith,
with the Securities and Exchange Commission, granting unto said attorney-in-fact and agent full power and authority
to do and perform each and every act and thing requisite and necessary to be done, as fully to all intents and purposes as she might or
could do in person, hereby ratifying and confirming all that said attorney-in-fact and agent, or her substitute or
substitutes, may lawfully do or cause to be done by virtue hereof.
Pursuant to the requirements of the Securities Act of 1933, as amended,
this registration statement has been signed below by the following persons in the capacities and on the dates indicated.
Name
Position
Date
/s/ Meeshanthini
    V. Dogan
Meeshanthini V. Dogan
Chief Executive Officer, Director
(principal executive officer)
March 22, 2023
/s/ Elisa Luqman
Elisa Luqman
Chief Financial Officer
(principal financial officer and principal accounting
    officer)
March 22, 2023
/s/ Warren Hosseinion
Director, Chair
March 22, 2023
Warren Hosseinion
/s/ James Intrater
James Intrater
Director
March 22, 2023
/s/ Stanley M.
    Lau
Stanley M. Lau
Director
March 22, 2023
/s/ Oded Levy
Oded Levy
Director
March 22, 2023
/s/ Robert Philibert
Robert Philibert
Director
March 22, 2023
/s/ Brandon Sim
Director
March 22, 2023
Brandon Sim
5