old_contents: # Plot Empirical Cumulative Distribution Function for Object Size
  
  import os
  import cv2
  import pandas as pd
  from plantcv.plantcv import params
  from plantcv.plantcv._debug import _debug
  from statsmodels.distributions.empirical_distribution import ECDF
  from plotnine import ggplot, aes, geom_point, labels, scale_x_log10
  
  
  def obj_size_ecdf(mask, title=None):
      """ Plot empirical cumulative distribution for object size based on binary
      mask
  
      Inputs:
      mask  = binary mask
      title = a custom title for the plot (default=None)
  
      Returns:
      fig_ecdf = empirical cumulative distribution function plot
  
      :param mask: numpy.ndarray
      :param title: str
      :return fig_ecdf: plotnine.ggplot.ggplot
      """
  
      objects, hierarchy = cv2.findContours(mask, cv2.RETR_TREE, cv2.CHAIN_APPROX_NONE)[-2:]
      areas = [cv2.contourArea(cnt) for cnt in objects]
      # Remove objects with areas < 1px
      areas = [i for i in areas if i >= 1.0]
  
      ecdf = ECDF(areas, side='right')
  
      ecdf_df = pd.DataFrame({'object area': ecdf.x[1:], 'cumulative probability': ecdf.y[1:]})
      # create ecdf plot and apply log-scale for x-axis (areas)
      fig_ecdf = (ggplot(data=ecdf_df, mapping=aes(x='object area', y='cumulative probability'))
                  + geom_point(size=.1)
                  + scale_x_log10())
      if title is not None:
          fig_ecdf = fig_ecdf + labels.ggtitle(title)
  
      # Plot or print the ecdf
      _debug(visual=fig_ecdf,
             filename=os.path.join(params.debug_outdir, str(params.device) + '_area_ecdf.png'))
      return fig_ecdf
  
new_contents: # Plot Empirical Cumulative Distribution Function for Object Size
  
  import os
  import cv2
  import pandas as pd
  from plantcv.plantcv import params
  from plantcv.plantcv._debug import _debug
  from statsmodels.distributions.empirical_distribution import ECDF
  from plotnine import ggplot, aes, geom_point, labels, scale_x_log10
  
  
  def obj_size_ecdf(mask, title=None):
      """ Plot empirical cumulative distribution for object size based on binary
      mask
  
      Inputs:
      mask  = binary mask
      title = a custom title for the plot (default=None)
  
      Returns:
      fig_ecdf = empirical cumulative distribution function plot
  
      :param mask: numpy.ndarray
      :param title: str
      :return fig_ecdf: plotnine.ggplot.ggplot
      """
  
      objects, _ = cv2.findContours(mask, cv2.RETR_TREE, cv2.CHAIN_APPROX_NONE)[-2:]
      areas = [cv2.contourArea(cnt) for cnt in objects]
      # Remove objects with areas < 1px
      areas = [i for i in areas if i >= 1.0]
  
      ecdf = ECDF(areas, side='right')
  
      ecdf_df = pd.DataFrame({'object area': ecdf.x[1:], 'cumulative probability': ecdf.y[1:]})
      # create ecdf plot and apply log-scale for x-axis (areas)
      fig_ecdf = (ggplot(data=ecdf_df, mapping=aes(x='object area', y='cumulative probability'))
                  + geom_point(size=.1)
                  + scale_x_log10())
      if title is not None:
          fig_ecdf = fig_ecdf + labels.ggtitle(title)
  
      # Plot or print the ecdf
      _debug(visual=fig_ecdf,
             filename=os.path.join(params.debug_outdir, str(params.device) + '_area_ecdf.png'))
      return fig_ecdf
  
subject: Remove unused variable