Histological analyses of apoptosis , proliferation , angiogenesis and infarct zone assessments were performed using terminal deoxynucleotidyltransferase-mediated dUTP nick-end labelling ( TUNEL ) staining , BrdU immunostaining , a-smooth muscle actin ( a-SMA ) /CD31 immunostaining and Masson 's trichrome staining , respectively .
O O O O O O O O O B-DiseaseOrPhenotypicFeature O O O O O B-GeneOrGeneProduct I-GeneOrGeneProduct B-ChemicalEntity O O O O O O O O O O B-GeneOrGeneProduct I-GeneOrGeneProduct I-GeneOrGeneProduct O B-GeneOrGeneProduct O I-GeneOrGeneProduct O O O O O O O O O