We screened germline mutations in the coding exons and the flanking intron sequences of the GATA4 gene in 486 CHD patients by denaturing high-performance liquid chromatography ( DHPLC ) , and confirmed the mutations by sequencing .
O O O O O O O O O O O O O O O B-GeneOrGeneProduct O O O B-DiseaseOrPhenotypicFeature B-OrganismTaxon O O O O O O O O O O O O O O O O