DNA was isolated from peripheral leukocytes and the region of interest in the AGT gene bearing a missense mutation methionine to threonine substitution at codon 235 ( M235T ) of exon 2 , was amplified by polymerase chain reaction ( PCR ) .
O O O O O O O O O O O O O B-GeneOrGeneProduct O O O O O B-SequenceVariant I-SequenceVariant I-SequenceVariant I-SequenceVariant I-SequenceVariant I-SequenceVariant I-SequenceVariant O B-SequenceVariant O O O O O O O O O O O O O O O