METHODS-RESULTS : Compared with the wild-type hGRalpha , the mutant receptor hGRalphaD401H demonstrated a 2.4-fold increase in its ability to transactivate the glucocorticoid-inducible mouse mammary tumor virus promoter in response to dexamethasone but had similar affinity for the ligand ( dissociation constant = 6.2 +/- 0.6 vs. 6.1 +/- 0.6 nm ) and time to nuclear translocation ( 14.75 +/- 0.25 vs. 14.25 +/- 1.13 min ) .
O O O O O O B-GeneOrGeneProduct O O O O B-GeneOrGeneProduct O O O O O O O O O O B-ChemicalEntity B-OrganismTaxon I-OrganismTaxon I-OrganismTaxon I-OrganismTaxon O O O O B-ChemicalEntity O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O