However , in transient transfection assays , the E333D TRbeta mutant exhibited impaired transcriptional regulation on two distinct positively regulated thyroid response elements ( F2- and DR4-TREs ) as well as on the negatively regulated human TSHalpha promoter .
O O O O O O O O B-SequenceVariant B-GeneOrGeneProduct O O O O O O O O O O O O O O O O O O O O O O O O O B-OrganismTaxon B-GeneOrGeneProduct O O