Molecular dynamic ( MD ) simulations showed that the overall effect of the mutation p.Val204Asp is disruption of hydrogen bonding between Gln223 and Glu441 , leading Ser198 and His438 to move away from each other with subsequent disruption of the catalytic triad functionality regardless of the type of substrate .
O O O O O O O O O O O O O O B-SequenceVariant O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O