A new and highly specific antibody ( A82 , Amgen ) was used to evaluate the presence of Epo-R by western blot analysis in addition to Epo-R signaling proteins ( Akt , STAT5 , p70s6k , LYN , and p38MAPK ) , activation of lipolytic pathways ( ATGL , HSL , CGI-58 , G0S2 , Perilipin , Cidea , Cidec , AMPK , and ACC ) , and mitochondrial biogenesis ( VDAC , HSP90 , PDH , and SDHA ) .
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