Markedly reduced or absent expression of Nf1 protein was observed in 6 of the 18 cell lines , and using the protein truncation test and sequencing of cDNA and genomic DNA , NF1 mutations resulting in deletion of exons and/or aberrant splicing of NF1 transcripts were detected in 5 of the 6 cell lines with loss of NF1 expression .
O O O O O O B-GeneOrGeneProduct O O O O O O O O O O O O O O O O O O O O O O O O O B-GeneOrGeneProduct O O O O O O O O O O B-GeneOrGeneProduct O O O O O O O O O O O O O B-GeneOrGeneProduct O O