In hRMVECs transfected with NOX4 siRNA and treated with VEGF or control , 1 ) ROS generation was measured using the 5- ( and-6 ) -chloromethyl-2',7'-dichlorodihydrofluorescein diacetate , acetyl ester fluorescence assay and 2 ) phosphorylated VEGF receptor 2 and STAT3 , and total VEGFR2 and STAT3 were measured in western blot analyses .
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