Toxicity of CAA was determined by protein content , cell number , LDH release , trypan blue exclusion assay and caspase-3 activity .
B-DiseaseOrPhenotypicFeature O B-ChemicalEntity O O O O O O O O O B-ChemicalEntity O O B-ChemicalEntity I-ChemicalEntity O O O B-GeneOrGeneProduct O O