Sanger sequence analysis of PTPN11 coding regions in a total of 17 MC families identified mutations in 10 of them ( 5 frameshift , 2 nonsense , and 3 splice-site mutations ) .
O O O O B-GeneOrGeneProduct O O O O O O O B-DiseaseOrPhenotypicFeature O O O O O O O O O O O O O O O O O O O O