We expressed both the major ( Lys-type ) and minor type ( Asn-type ) preproET-1 in three different cell lines , and measured the levels of ET-1 and big ET-1 in the culture supernatant .
O O O O O O O O O O O O O O I-GeneOrGeneProduct O O O O O O O O O O O B-GeneOrGeneProduct O O B-GeneOrGeneProduct O O O O O