Co-transfection experiment of both wild-type and mutant plasmids indicated the dominant-negative mechanism of disease development ; as more of mutant DNA was transfected , VWF secretion was impaired in the media , whereas more of VWF was stored in the cell lysates .
O O O O O O O O O O O O O O O O O O O O O O O O B-GeneOrGeneProduct O O O O O O O O O O B-GeneOrGeneProduct O O O O O O O