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Physics
Condensed Matter / Superconductivity
Numerical Values
Low-Gap Hf–HfOx–Hf Josephson Junctions for meV-Scale Particle Detection
https://arxiv.org/abs/2510.25203v1
Oct 29, 2025
Researchers fabricated and characterized Josephson junctions made of hafnium (Hf), a low-Tc superconductor. The JJs employed a Manhattan-style geometry with varying junction overlap areas, with leads of approximately 200 nm. For junction fabrication, the silicon wafer was first spun with MMA e-beam resist at a speed of...
- Current (nA) across the Hf junction at 13 mK under applied voltages ranging from -65 to +65 µV.
Researchers fabricated and characterized Josephson junctions made of hafnium (Hf–HfOx–Hf). The device was mounted in a dilution refrigerator at cryogenic temperatures. The junction was connected in series with a voltage divider circuit to control the voltage sweep, and the resulting current was measured using a DC SQUI...
[5.6-6.8] nA Note: No CI/SE/SD provided -> a fallback of ±10% was applied to the 6.2 nA value provided by the authors.
- In several qubit-derived detector architectures, absorbed energy generates non-equilibrium quasiparticles that tunnel across the JJ, producing measurable changes in the qubit’s parity or resonance frequency. - Using superconductors with lower critical temperature, Tc, and smaller superconducting gap enhances detector...
[{"label":"RBK Item","value":"Using superconductors with lower critical temperature, Tc, and smaller superconducting gap enhances detector responsivity by increasing the quasiparticle population and lifetime per absorbed energy."},{"label":"Title","value":"Relaxation and frequency shifts induced by quasiparticles in su...
Biology
RNA Interference, Cell Signaling, Microgravity Research
Free-Format Question
Targeting miR-337 mitigates disuse-induced bone loss
https://www.nature.com/articles/s41421-025-00822-z
August 26, 2025
Researchers exposed two-month-old male Sprague-Dawley rats to hindlimb unloading (HU). To do this, they applied a strip of medical adhesion tape along the proximal one-third of tails and attached these to metal bars at cage tips. They adjusted the suspension height to prevent the hindlimbs from touching any supporting ...
- Relative miR-337 expression levels (Fold change) in LepR+ cells from hindlimb unloading (HU) and weight-bearing (WB) control rats by quantitative RT-PCR.
Researchers exposed two-month-old male Sprague-Dawley rats to hindlimb unloading (HU) for 7 days. Mesenchymal stem cells (MSCs) were isolated from the femurs and tibiae of HU and weight-bearing (WB) control rats by flushing bone marrow (BM) cells in growth medium and culturing them until confluence. Leptin receptor-pos...
Hindlimb unloading (HU) rats
- MicroRNA-337-3p (miR-337) is a mechanical stress-sensitive regulator that mediates osteogenic differentiation through the direct targeting of insulin receptor substrate 1 (IRS-1). - Leptin receptor (LepR) positive mesenchymal stem cells (MSCs) have shown to be capable of mechanical sensing. - MicroRNAs are small nonc...
[{"label":"RBK Item","value":"MicroRNA-337-3p (miR-337) is a mechanical stress-sensitive regulator that mediates osteogenic differentiation through the direct targeting of insulin receptor substrate 1 (IRS-1)."},{"label":"Title","value":"Overexpression of mechanical sensitive miR-337-3p alleviates ectopic ossification ...
Biology
Cellular Neuroscience
Numerical Values
All-optical voltage interrogation for probing synaptic plasticity in vivo
https://www.nature.com/articles/s41467-025-63867-4
Oct 3, 2025
The authors evaluated the peak response amplitude of a new genetically encoded voltage indicator (GEVI), JEDI-2Psub, with a tryptophan insertion between the GFP subunit and the voltage-sensing domain of JEDI-2P. The GEVI was cloned into a pcDNA3.1/Puro-CAG vector and transfected into HEK293A cells plated on a 30-70 kD ...
- Peak response amplitude to spike waveforms (ΔF/F0) in JEDI-2Psub cells.
A new genetically encoded voltage indicator (GEVI), JEDI-2Psub, was developed with a tryptophan insertion between the GFP subunit and the voltage-sensing domain of JEDI-2P. We perform a two-photon microscopy recording of plated HEK293A cells transfected with JEDI-2Psub under voltage clamp with a glass micropipette with...
ΔF/F0 = [-40.9 - -27.3]% derived from the spike voltage response −34.1%. Reported ± 6.8% mean ± 95% CI.
- Genetically encoded voltage indicators (GEVIs) are suitable for two-photon microscopy and offers the prospect of measuring voltage signals directly in networks of genetically identified neurons in vivo. - Two-photon microscopy in vivo records somatic calcium signals as a proxy for action potentials, and dendritic cal...
[{"label":"RBK Item","value":"- Genetically encoded voltage indicators (GEVIs) are suitable for two-photon microscopy and offers the prospect of measuring voltage signals directly in networks of genetically identified neurons in vivo."},{"label":"Title","value":"Sustained deep-tissue voltage recording using a fast indi...
Biology
Immunology/Lipid nanoparticle vaccines
Numerical Values
Anionic lipids modulate mRNA-lipid nanoparticle immunogenicity and confer protection in a mouse model of multiple sclerosis
https://www.biorxiv.org/content/10.1101/2025.10.17.683125v1
October 17, 2025
mRNA at a concentration of 1mg/mL was supplied by GenScript, linearized, purified, and subjected to in-vitro transcription using MEGAscript T7 transcription kit using co-transcriptional capping with CleanCap. Uridine was fully substituted with N1-methylpseudouridine before mRNAs were purified by cellulose chromatograph...
-%GFP+ cells among T-cells, B-cells, natural killer cells, neutrophils, macrophages, and dendritic cells in the C57BL/6 mouse spleens 18 hours after IV administration of 40 chemically distinct GFP mRNA LNP formulas
10ug of green fluorescent protein (GFP) mRNA encoding lipid nanoparticles (LNPs) formulated with SM-102 + DOPS in a molar ratio of 50:10:20:18:2 ionizable lipid:DSPC:anionic lipd:chloestrol:DMG-PEG2000 is infused into a C57BL/6 mouse, and the spleen was harvested 18 hours later. Cells are isolated from the spleen by sq...
%GFP+ Spleenic DCs = [11.5 - 21.5] % derived from approximately 16.5% of splenic DCs with SM-102 + DOPS in a molar ratio of 50:10:20:18:2 expressing GFP. Note: No CI/SE/SD reported -> fallback ±5 pp applied
- Selective organ targeting (SORT), leverages the incorporation of anionic lipids to redirect mRNA–LNPs from the liver to lymphoid tissues - Selective organ targeting (SORT) enhances mRNA delivery broadly to immune cells—including macrophages, dendritic cells, and lymphocytes—within the spleen and lymph nodes.
[{"label":"RBK Item","value":"- Selective organ targeting (SORT), leverages the incorporation of anionic lipids to redirect mRNA–LNPs from the liver to lymphoid tissues"},{"label":"Title","value":"Selective organ targeting (SORT) nanoparticles for tissue-specific mRNA delivery and CRISPR–Cas gene editing\n"},{"label":"...
Biology
Skeletal Muscle Physiology, Space Medicine
Free-Format Question
Long non-coding RNAs Kcnq1ot1 and Lncpint are involved in skeletal muscle atrophy induced by the space exposome
https://physoc.onlinelibrary.wiley.com/doi/10.1113/JP288987
June 29, 2025
Researchers used a 3D human-derived cellular model (spheroids) of skeletal muscle atrophy to provide in vivo evidence for the involvement of specific lncRNAs in muscle adaptations to the space exposome. For spheroid development, tissue obtained from vastus lateralis muscle biopsies from healthy donors was minced, dige...
- LINC-PINT expression in human-derived muscle spheroids treated with myostatin versus vehicle control (qPCR) - KCNQ1OT1 expression in human-derived muscle spheroids treated with myostatin versus vehicle control (qPCR)
Researchers investigated the expression of long non-coding RNAs (lncRNAs) KCNQ1OT1 and LINC-PINT in a spheroid model of human skeletal muscle atrophy. For spheroid development, vastus lateralis muscle biopsies were obtained from healthy donors. After the spheroids reached maturity following 5 days of growth and 10 days...
KCNQ1OT1 exhibited significantly higher expression in myostatin-treated spheroids compared to vehicle-treated controls, but LINC-PINT expression did not differ between control and myostatin-treated groups.
- Long non-coding RNAs (lncRNAs) are emerging as important actors in skeletal muscle transcriptional control - KCNQ1OT1 (KCNQ1 overlapping transcript 1) is a macro lncRNA involved in epigenetic gene silencing, whose overexpression impedes MyoD1 function and disrupts the myogenic program. - LINC-PINT is a p53-induced l...
[{"label":"RBK Item","value":"Long non-coding RNAs (lncRNAs) are emerging as important actors in skeletal muscle transcriptional control "},{"label":"Title","value":"Functions and Therapeutic Potentials of Long Noncoding RNA in Skeletal Muscle Atrophy and Dystrophy"},{"label":"URL","value":"https://onlinelibrary.wiley....